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Purification and Characterization of $\beta$-Xylosidase from Trichoderma sp. SY  

Kim, Bong-Gyu (Department of Forest and Environmental Science, Bio/Molecular Informatics Center, Konkuk University)
Jung, Bo-Ra (Department of Advanced Fusion Technology, Bio/Molecular Informatics Center, Konkuk University)
Jung, Jae-Gyu (Department of Forest and Environmental Science, Bio/Molecular Informatics Center, Konkuk University)
Hur, Hor-Gil (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology)
Ahn, Joong-Hoon (Department of Forest and Environmental Science, Department of Advanced Fusion Technology, Bio/Molecular Informatics Center, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.3, 2004 , pp. 643-645 More about this Journal
Abstract
A $\beta$-xylosidase was purified from the culture of Trichoderma sp. SY. The ten-day-old culture filtrate was concentrated, followed by ion-exchange chromatography and gel filtration chromatography. As a result, $\beta$-xylosidase was finally purified about 53-fold and appeared as a single band by SDS-PAGE. The optimum pH and temperature were 5.0 and $55^{\circ}C$, respectively, and the molecular weight about 80 kDa. The purified $\beta$-xylosidase was found to be inhibited by various metal ions but not inhibited by xylose.
Keywords
$\beta$-Xylosidase; Trichoderma;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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