• BMB Reports
• /
• v.39 no.1
• /
• pp.76-83
• /
• 2006

Pyridoxine-5-P oxidase catalyses the terminal step in the biosynthesis of pyridoxal-S-P, the biologically active form of vitamin $B_6$ Which acts as an essential cofactor. Here, a human brain pyridoxine-5-P oxidase gene was fused with a gene fragment encoding the HIV-1 Tat protein transduction domain (RKKRRQRRR) in a bacterial expression vector to produce a genetic in-frame Tat-pyridoxine-5-P oxidase fusion protein. Expressed and purified Tat-pyridoxine-5-P oxidase fusion protein transduced efficiently into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced Tat-pyridoxine-5-P oxidase protein showed catalytic activity and was stable for 48 h. Moreover, the formation of pyridoxal-5-P was increased by adding exogenous Tat-pyridoxine-5-P oxidase to media pre-treated with the vitamin $B_6$ precursor pyridoxine. In addition, the intracellular concentration of pyridoxal-S-P was markedly increased when Tat-pyridoxal kinase was transduced together with Tat-pyridoxine-5-P oxidase into cells. These results suggest that the transduction of Tat-pyridoxine-5-P oxidase fusion protein presents a means of regulating the level of pyridoxal-5-P and of replenishing this enzyme in various neurological disorders related to vitamin $B_6$.

• Korean Journal of Veterinary Research
• /
• v.33 no.3
• /
• pp.547-554
• /
• 1993

This study was carried out to investigate the best condition for in vitro and in vivo culture after freezing and thawing of nuclear transplant 2 cell embryos. When nuclear transplant embryos were submitted to electrofusion, the significantly higher fusion rates of 2 cell donor nuclei were achieved at the electric field strength of DC 1.5 kV/cm for 100 and $150{\mu}sec$, DC 2.0 kV/cm for 100 and $150{\mu}sec$ than DC 1.0 kV/cm for 100 and $150{\mu}sec$(p<0.01). The significantly higher fusion rates of 4 cell donor nuclei were achievecl at DC 2.0 kV/cm for 100 and $150{\mu}sec$ than DC 1.0 kV/cm for 100 and $150{\mu}sec$(p<0.01). The fusion rates in 8 cell donor nuclei were 94.2~99.3%. The developmental potency to blastocyst in 2 cell donor nuclei was significantly higher in DC 2.0 kV/cm for $150{\mu}sec$ treated group(p<0.01). The significantly higher developmental potency to blastocyst in 4 cell donor nuclei were achieved at the electric field strength of DC 2.0 kV/cm for $150{\mu}sec$ than DC 1.5 kV/cm for 100 and $150{\mu}sec$, DC 2.0 kV/cm for $100{\mu}sec$ treated group(p<0.01). The develop mental potency to blastocyst in 8 cell donor nuclei was significantly higher in DC 2.0 kV/cm for $100{\mu}sec$ treated group(p<0.01). The developmental potency to blastocyst after nuclear transplantation was significantly higher in 2 cell donor nuclei than in 8 cell donor nuclei(p<0.01). When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant(p<0.01). The production rates of offspring after transfer of nuclear transplant embryos to recipient mouse were no significant difference in 2, 4 and 8 cell donor nuclei.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.11
• /
• pp.1881-1890
• /
• 2016

Manganese superoxide dismutase (MnSOD) is a vital enzyme that protects cells from free radicals through eliminating superoxide radicals ($O^{2-}$). Hirudin, a kind of small active peptide molecule, is one of the strongest anticoagulants that can effectively cure thrombus diseases. In this study, we fused Hirudin to the C terminus of human MnSOD with the GGGGS linker to generate a novel dual-feature fusion protein, denoted as hMnSOD-Hirudin. The hMnSOD-Hirudin gene fragment was cloned into the pET15b (SmaI, CIAP) vector, forming a recombinant pET15b-hMnSOD-Hirudin plasmid, and then was transferred into Escherichia coli strain Rosetta-gami for expression. SDS-PAGE was used to detect the fusion protein, which was expected to be about 30 kDa upon IPTG induction. Furthermore, the hMnSOD-Hirudin protein was heavily detected as a soluble form in the supernatant. The purification rate observed after Ni NTA affinity chromatography was above 95%. The hMnSOD-Hirudin protein yield reached 67.25 mg per liter of bacterial culture. The identity of the purified protein was confirmed by western blotting. The hMnSOD-Hirudin protein activity assay evinced that the antioxidation activity of the hMnSOD-Hirudin protein obtained was $2,444.0{\pm}96.0U/mg$, and the anticoagulant activity of the hMnSOD-Hirudin protein was $599.0{\pm}35.0ATU/mg$. In addition, in vitro bioactivity assay showed that the hMnSOD-Hirudin protein had no or little cytotoxicity in H9c2, HK-2, and H9 (human $CD_4{^+}$, T cell) cell lines. Transwell migration assay and invasion assay showed that the hMnSOD-Hirudin protein could suppress human lung cancer 95-D cell metastasis and invasion in vitro.

• Journal of the Daesoon Academy of Sciences
• /
• v.42
• /
• pp.143-165
• /
• 2022

This study is a novel attempt at a fusion of female-authored Chinese poetry and Daesoon Thought. Notably, this has style of fusion has never been attempted in classical literature studies or in studies on Daesoon Thought. This study will also clarify the the key concept of grievance-resolution (解冤 haewon) in Daesoon Jinrihoe through comparison with classical works. Choi Song-seoldang's poetry that was composed in the early 20th century, contemporaneous the emergence of Daesoon Thought, is analyzed here via the concepts of 'tranquility (平 pyeong)' and 'harmony (和 hwa)' under the framework of grievance-resolution for women. An effort is made to find a point of progression towards familial harmony (家和 gahwa) and Mutual Beneficence (相生 sangsaeng). Resentment (恨 han) from the perspective of a woman was expressed in her works such as Wang So-gun's Resentment (昭君怨 sogunwon), Self-Report (自述 Jasul), and An Original Rhyme of Song Seol-dang (松雪堂原韻 Song Seol-dang Won-un). Works such as Wishes on the First Day of New Year (元朝祝 wonjochuk) and A Spontaneous Poem (偶吟 Ueum) expressed the contents of wishing for familial peace. In the process of trying to resolve the grievances (冤 won) of her family, Song Seol-dang faced limitations, and felt resentment (恨 han) for her inability to become a man. She strived her whole life to embody 'tranquility' and 'harmony' as both are crucial components of achieving familial harmony. This thesis has an important significance in terms of academic expansion via the convergence of literature and ideas. In terms of Daesoon Thought, it is meaningful to examine concepts and literature within a context of fusion because this goes beyond research that focuses only on theory or ideology. It is also meaningful to confirm aspects of Daesoon Thought through the life and culture of the still traditional early modern era and to reveal how it still has the present-day significance that transcends time.

• Korea Science and Art Forum
• /
• v.24
• /
• pp.399-409
• /
• 2016

This study's purpose is to research the correlation between property and the major festivals with media art of exemplary 3 cities among 8 Unesco Media Art Creative Cities. As a result, it is drawn a conclusion that the below strategies are necessary to achieve success for Unesco Media Art Creative City. First, from the Creative City's point of view, on the basis of the maximization of various merits such as history, natural surroundings, and human resources of the city, it is necessary to reconcile a vision of the city and an identity of Media Art Creative City, and to seek for continuous growth of the city by consistency and durability of control tower within the local government. Second, from the local festival's point of view, it is necessary to provide a differentiated service in the existing media art festivals, to attract the fusion of media art and festivals, and the diversification and the popularization of media art programs. And, it needs to lead to involvement of young people with the fusion of on-line and off-line festivals, the fusion of analogue and digital festivals. In the conclusion, for the successful constructing of Media Art Creative City, it is necessary to maximize the merits of the city's own, to enhance the image for Media Art Creative City with the correlation between the merits and media art, and to differentiate the city's own various festivals, ant the same time, to converge them with media art technology. With these strategies, the local festival can make a new leap for global festival when it constitutes a whole with Media Art Creative City.

• Journal of Mushroom
• /
• v.19 no.3
• /
• pp.166-175
• /
• 2021

In this study, monokaryons of "Heukari" (Pleurotus ostreatus) and "Hosan" (Pleurotus pulmonarius) were separated to remove the cell wall, and a cross-species protoplast fusion was developed through chemical treatment with polyethylene glycol. The protoplast-fused PF160306 and PF160313 strains have a culture period of 10 and 2 days shorter than that of the "Heuktari" and "Hosan" cultivars, respectively. Furthermore, the growth of the strains was faster than that of the existing cultivars. The yield was 135.9 g per bottle, which was approximately 8% higher than that of the commercially available "Hosan" cultivar; however, it was not statistically significant. A growth survey was conducted after treatment at five temperatures (15, 18, 21, 23, and 25℃). The growth of the strains accelerated with the increase in temperature. However, at 21℃, the yellow color of pileus was the brightest. Band pattern, assessed using URP Primer 7, was similar to that of the "Hosan" cultivar. The DPPH radical scavenging capacity and polyphenol content were 62.5% and 43.5 mg/mL, respectively, for "Sunjung" and 65.7% and 49.9 mg/mL, respectively, for PF160313. Furthermore, the antihypertensive activities of the "Sunjung" cultivar and PF160313 were similarly high at 74% and 75%, respectively. In conclusion, cross-species hybridization via the protoplast fusion technique can be used for obtaining primary data for mushroom breeding to develop new varieties. In addition, the protoplast fusion technique might aid in expanding the market for yellow mushrooms.

• The Research Journal of the Costume Culture
• /
• v.19 no.6
• /
• pp.1288-1301
• /
• 2011

The purpose of this study was to observe a case study of pop-up stores in an apparel company in Korea in order to evaluate different feel and to determine the purchase behavior of pop-up visitors of three major fashion brands. This research was conducted through secondary data collection and primary data collection. A survey was conducted among NIKE, KUHO, and PUMA pop-up store visitors who were 20 to 40 years old, via questionnaires. Data were collected on-line and off line at the pop-up store. Data were analyzed using SPSS program. Through data analysis, we learned that most of the study participants were captivated by the digital elements in the environment of the NIKE pop-up store. Puma, the second most favorably ranked, was favored for its interior design. Meanwhile, KUHO stimulated its customers with its limited edition fashion products at a good price. Several feels of visitors about the pop-up stores were significantly different among the 3 different brands. There was a significant difference in purchase criteria, such as brand recognition, size, and design among visitors of the three different brands. Visitors who preferred KUHO considered design of products more than NIKE-preferred visitors. In conclusion, the clothing pop-up stores have the possibility of satisfying consumers' desires. Each brand can achieve a good performance in promotion with a differentiated strategy.

• The Research Journal of the Costume Culture
• /
• v.26 no.1
• /
• pp.73-81
• /
• 2018

Developments in the recent media era has made distortion possible in accordance with changes in the fashion industry as new clothing, technologies, and fusions have become available. Nevertheless, previous studies of specific types of clothing, and their classification for the purpose of research are lacking. Thus, this study examines the development of the Pomme de look and the form of modern variant that appears in fashion clothing and new technologies, and largely examines the clothing case to clean it up. The method employed is leading research that examines the transformation about the concept of the Pomme de look with regard to the fashion about the type of look in the 2000s and onwards. This is organized in accordance with the analytical and physical characteristics, and the esthetic aspects of the results are as follows. The conclusions reached as follows are based on this; first, the physical characteristic manner when based on previous looks, the two-dimensional deformation for making clothes, if there was a simple way, now has more dimensions. Clothes are made in a complex manner and variations emerge. Second, esthetic characteristics strive for beauty while also displaying the modified and more complex manners of clothing that are emerging. Third, the fusion clothing with machines effects to physical and psychological changes through the details about how the sensor is worn. The enemy, the appearance of distortion, and clothing are emerging. This is expected to grow further based on the rapid development of new technologies and the transformation of Pomme de look and design will appear in more varied ways to create a convergence.

• The Journal of the Convergence on Culture Technology
• /
• v.4 no.2
• /
• pp.211-216
• /
• 2018

Separation technology combining adsorption and membrane is expected to be applied in many fields such as water treatment. In this fusion technique, a functional carbon membrane having a carbon whisker grown on the surface of the membrane was developed to inhibit membrane fouling, which is a problem in the membrane separation process. In this study, to elucidate the mechanism of manufacturing the functional carbon membrane, the membrane was pretreated with the polymer latex of each mixing ratio and the membrane was formed by the CVD (Chemical Vapor Deposition) method. The membrane was analyzed by scanning electron microscope (SEM), CHN analyzer (Elemental Analyzer), and X-ray diffraction (XRD). As a result, the diameter and density of carbon whiskers were higher in case of polyvinyl di-chloride (PVdC): polyvinyl chloride (PVC) = 4.5: 55. It seems possible to control the diameter and density of the carbon whiskers according to the hydrogen content of the polymer latex.

• The Research Journal of the Costume Culture
• /
• v.24 no.5
• /
• pp.626-641
• /
• 2016

Maximalism in modern fashion, which is contrary to the current fashion market that emphasizes functionality and practicality, is an important notion that has been expressed in a variety of ways through designer's unique sense with a mixture of experimental design. With such creative work that suits a trend in novel fashion design, Rei Kawakubo is a current representative of Comme des Garcons who has not only introduced deconstructivism and Japanese-style avant-garde to the fashion industry for the first time. Our research, thus, analyzed the characteristic of maximalism's expression based on the features represented in her collection. Firstly, Expandability is classified as a method of distorting parts of a body and a method of excessively overinflating the silhouette of clothing. Secondly, decorations are expressed in the other as blending different types of materials together. Thirdly, fantasy evokes a fresh and stimulating surreal sensation that may only be felt in an imagination by assigning supernatural and amusing expression to the garment. The forth, fusion refers to a creation of novelty from combining various types of elements and images. The fifth, experiment is unique trait that develops mysterious images, consisted of various details. The sixth, non-structure is different from an initial form of garment that maintains a bilateral symmetry. The importance of our research lies in proposing a fundamental data for development of creative design as well as in expanding a range of possible expression for maximalism, by analyzing the characteristics of maximalism seen in Rei Kawakubo's collection.