• 제목/요약/키워드: Culture Fusion

검색결과 484건 처리시간 0.026초

Retinoic Acid가 배양게배 근원세포의 융합과 Fibronictin의 발현에 미치는 영향 (Effect of Retinoic Acid on Membrane Fusion and Expression of Fibronectin in Chick Embryonic Myoblasts)

  • 김혜선;정필중;강만식;정진하;하두봉
    • 한국동물학회지
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    • 제38권4호
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    • pp.483-489
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    • 1995
  • 많은 세포와 조직에서 분화 조절물질로 알려져 있는 비타민 A의 대사산물인 rectinoic acid(RA)가 배양 계배 근원세포의 성장과 분화에 미치는 영향을 조사하였다. RA가 처리된 세포는 그 농도가 증가함에 따라 융합 억제 효과도 증가함을 보였고, 배양액으로부터 RA를 제거해 주면 근원세포의 융합이 재개되는 것으로 보아, RA는 근원세포의 융합을 가역적으로 억제함을 알 수 있었다. 그러나 이미 최종 분화 단계에 들어선 세포에서는 융합 억제 효과를 보이지 않아, RA의 효과는 분화 단계에 따라 특이적으로 작용함을 보여준다. 융합 억제 효과에도 불구하고, RA는 근원세포의 융합 전단계인 방추형으로 신장하는 것과 그들이 가상의 축을 따라 배열하는 데는 영향을 주지 않았다. 또한 세포의 증식과 creatine kimase, tropomyosin과 같은 근특이 단백질의 축적에도 큰 영향을 주지 않았다. 한편, 근원세포는 융합하기 전에 필수적으로 fibronectin의 감소를 억제함을 알 수 있었다. 이상의 결과로 RA는 배양 근원세포의 융합만을 특이하게 억제하는 물질이며, 그 작용은 fibronectin의 감소를 방해하는 것과 밀접한 관계가 있는 것으로 사료된다.

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Production of Recombinant Polyhedra Containing Cry1Ac Fusion Protein in Insect Cell Lines

  • Kim, Jae-Su;Choi, Jae-Young;Roh, Jong-Yul;Lee, Han-Young;Jang, Seung-Sik;Je, Yeon-Ho
    • Journal of Microbiology and Biotechnology
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    • 제17권5호
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    • pp.739-744
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    • 2007
  • Insect cell lines and the control of infection for obtaining the maximum amount of polyhedrin-Cry1Ac-polyhedrin fusion protein from Bactrus in monolayer and suspension culture systems were tested. Growth rates of the Trichoplusia ni(High-Five) cell line in both culture systems were better than the other insect cell lines, Spodoptera frugiferda(Sf-9, Sf-21), Trichoplusia ni(Tn5), and Spodoptera exigua(Se301). The expression of the fusion protein in a monolayer culture showed that Se301 cells were 2.3-4.8 times more productive on a per cell basis than the other cell lines. However, in suspension culture, only High-Five cells were productive. High-Five cells infected with Bactrus at a multiplicity of infection(MOI) of 5 and a cell density of $3.0{\times}10^5$ cells per ml were more productive than the other infection condition in a suspension culture suitable for a large-scale production of baculovirus. In conclusion, for the large-scale production of Bactrus in vitro, High-Five cells showing good growth and high productivity are suitable.

Adobe Illustrator 9.0을 이용한 의류용 텍스타일 디자인 개발-전통문양의 캐릭터화 사례를 응용하여- (A Study on Apparel Textile Design Process using Adobe Illustrator 9.0-Applying Pre-Developed Korean Traditional Motifs-)

  • 곽태기;양수영
    • 복식문화연구
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    • 제9권3호
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    • pp.501-510
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    • 2001
  • This study is focused on apparel textile design process applying pre-developed Korean traditional motifs. Since the fusion culture is in trend, Orientalism or Oriental look is the center of attraction in fashion. Under the circumstances, developing the traditional resources as sophisticated Korean-style is the goal of the BK21 team. This study makes a move to achieve the goal. As a method of accomplishing this study, 4 trendy motif samples such as stylized letter, butterfly, peony, and female face are selected after investigating fashion magazines and fashion related websites. As a result, pre-developted Korean traditional motifs are elected and revised using Adobe Illustrator 9.0. The newly revised motifs are applied to the textile pattern repeat form. The completed textile patterns are simulated on the simulated on the shawls to show their usages as one of souvenir items since the year of 2001 is the year of “Visit Korea”.

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퓨전디자인의 속성에 관한 연구 - 문화환경의 변화를 중심으로 - (A Study on the Properties of fusion Design -Especially on the Change of Cultural Environment-)

  • 박규현;김윤경
    • 디자인학연구
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    • 제13권3호
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    • pp.211-220
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    • 2000
  • 문화의 접속이란 특정한 문화가 영향력을 가지고 다른 문화로 이동하는 흐름을 나타내는 것인데 이것을 부정적으로 표현하면 문화의 '침투'이지만 긍정적으로 표현하면 문화의 '융합'혹은 '퓨전(Fusion)'이 된다. 과거에는 민족주의와 국수주의가 사상적으로 이를 불허해왔고, 또 교통과 통신의 불편은 여기에 장애물로 작용했으며, 대부분 토속종교에 영향받는 국가들이었으므로 문화의 융합이란 용이하지 않았다. 그러나 오늘날은 세계화로 인한 국가관의 붕괴, 지역성의 파괴, 무역장벽의 철폐, 통신의 자유, 정보의 네트워크와 실시간 등은 많은 면에서 상호 이질문화를 쉽게 공유할 수 있게 하였다. 바로 여기에 퓨전이 가능하게 되었다. 오늘날 현대소비사회에 있어서의 퓨전현상은 과거보다 더욱 빠르고 광범위하게 진행 되어간다. 그리고 소비의 기호가 세분화되고 다양한 개성을 찾아나가려는 시도가 강하게 나타나는 오늘의 상황에서 볼 때 앞으로 더욱 많은 문화인자들이 퓨전이라는 이름 하에 상호 충돌과 절충을 시도하면서 융합의 고삐를 늦추지 않을 것으로 본다. 본 연구는 현대의 이러한 새로운 대중문화 양식의 한 현상인 "퓨전(fusion)"에서 특히 사회과학적 문화현상을 중심으로 그 특성을 연구하고자 하였다. 그리고 디자인분야 중 문화현상을 가장 잘 표현하고 있는 실내디자인에서의 퓨전현상을 찾아 이를 학문적으로 규명하고, 최근의 주요 동향에 대한 고찰을 바탕으로 향후 퓨전디자인(Fusion Design)의 방향을 전망하도록하였다.

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Comparison of various image fusion methods for impervious surface classification from VNREDSat-1

  • Luu, Hung V.;Pham, Manh V.;Man, Chuc D.;Bui, Hung Q.;Nguyen, Thanh T.N.
    • International Journal of Advanced Culture Technology
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    • 제4권2호
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    • pp.1-6
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    • 2016
  • Impervious surfaces are important indicators for urban development monitoring. Accurate mapping of urban impervious surfaces with observational satellites, such as VNREDSat-1, remains challenging due to the spectral diversity not captured by an individual PAN image. In this article, five multi-resolution image fusion techniques were compared for the task of classifting urban impervious surfaces. The result shows that for VNREDSat-1 dataset, UNB and Wavelet tranformation methods are the best techniques in reserving spatial and spectral information of original MS image, respectively. However, the UNB technique gives the best results when it comes to impervious surface classification, especially in the case of shadow areas included in non-impervious surface group.

Expression of an Angiogenin Binding Peptide and Its Anti-Angiogenic Activity

  • Choi, Suk-Jung;Ahn, Mi-Won;Yoon, Kyoung-Bum;Park, Jong-Won
    • BMB Reports
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    • 제31권5호
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    • pp.427-431
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    • 1998
  • In the previous report (Choi et al., 1997), the angiogenin binding peptides identified from a phage-peptide library were analyzed by using the fusion proteins composed of the Escherichia coli maltose binding protein and its corresponding peptides. However, it was difficult to obtain a sufficient amount of the fusion proteins required for further analysis because of the low expression level. We now report a high level expression of the fusion protein and analysis of its anti-angiogenin activity. The use of strong T7 promoter and removal of signal sequence allowed about a 20-fold increase in the expression efficiency of the fusion protein. We were able to obtain about 10 mg of purified fusion protein from one liter of culture. The purified fusion protein showed angiogenin-specific affinity and inhibited the binding of biotinylated actin to human angiogenin at $IC_{50}$ of 0.6 mM. Its anti-angiogenin activity was also revealed by the chorioallantoic membrane assay.

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웨스턴 스타일 패션에 나타난 하이브리드 경향 (A Study on Hybrid Reflected on Western Style Fashion)

  • 한수연;양숙희
    • 복식문화연구
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    • 제17권4호
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    • pp.679-690
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    • 2009
  • The purpose of this study is to comprehend western style in fashion and to contemplate the hybrid tendency reflected on western style, thereby to analyze the aesthetic characteristics of the hybrid tendency of western style. For such purposes, this study first examines western style historically, in order to analyze hybrid tendency reflected on western style, and conduct a case study by analyzing photographic materials of pr t- -porter collection since 1980s. The result of this study is as follows: the hybrid tendency reflected on western style are modification and fusion of regional culture, adaptation and composition of subcultures, and appropriation of sexual minority culture. (1) Modification and fusion of regional culture is expressed in ethnic items and patterns of embroidery, mixing Mexican and Spanish, American Indian and American cultural references. (2) Adaptation and composition of subcultures emphasize traditional or vintage western style by mixing characteristics of western and other subcultures. (3) Appropriation of sexual minority culture is expressed in rhinestone chaps and fetish corset, glitter rodeo suit, reminiscent of drag or queer cultural references. The result of this study will provide basis which can be utilized in the development and educational background of fashion design.

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핵이식 기법을 이용한 한우 쌍태생산에 관한 연구 I. Ovum pick-up(OPU), 전기적 세포융합 및 체외배양 기법을 이용한 복제수정란 생산 (Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos I. Embryo cloning using ovum pick-up(OPU), electric cell fusion and in vitro culture system)

  • 황우석;신태영;노상호;이병천
    • 대한수의학회지
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    • 제38권1호
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    • pp.209-216
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    • 1998
  • The purpose of present study is to improve the efficiency of fusion and the developmental rates of nuclear transplanted embryos to produce genetically identical twins from Korean native cattle. The diameter of aspirated follicles had no significant effect on the recovery rates of oocytes collected by ovum pick-up technique. The fusion rates of nuclear transplanted embryos were significantly higher in 50 and $100{\mu}s$ DC duration groups(73.3 and 72.0% ; respectively) than that in $30{\mu}s$ group(55.6% ; p<0.05). The cleavage rates of nuclear transplanted embryos appeared to be significantly higher in donor nuclei derived from in vivo (65.0%) than in those from in vitro (50.5% ; p<0.01), but the developmental rates to morulae and blastocysts were not significantly different between them(13.7 vs 10.9%, respectively).

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Culture of Karyoplast and Cytoplast Complexes in High Osmolarity after Fusion Improve In Vitro Development of Porcine Nuclear Transfer Embryos

  • Im, Gi-Sun;Hwang, In-Sun;Kim, Dong-Hoon;Yang, Byoung-Chul;Kim, Se-Woong;Park, Hyo-Suk;Seo, Jin-Sung;Yang, Bo-Suk;Chang, Won-Kyong
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.291-291
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    • 2004
  • Micromanipulation and fusion are essential to generate nuclear transfer embryos. In this process cytoplasmic damage is unavoidable. This study investigated the hypothesis that higher osmolarity than normal culture medium could help oocytes recover from cytoplasmic damage from micromanipulation and electric pulse. Oocytes derived from a local slaughter house were matured for 42 ∼ 44 h and enucleated. (omitted)

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유연근무가 조직과 직무성과에 미치는 긍정적인 영향에 대한 연구 (M사 시차출퇴근 제도 도입 사례 중심으로) (Research on the positive impact of flexible work on organization and job performance (The case of introduction of M company time difference commuting system))

  • 이희;이만수
    • 대한안전경영과학회지
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    • 제19권4호
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    • pp.253-260
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    • 2017
  • Due to dramatic changes in the trend of corporate management in economics, labor and government, companies are being asked to adapt creative and innovative organizational culture in order to keep sustainability. Under the circumstances, flexible working hour becomes one of very effective method for organization culture improvement. Recently, M company has introduced the new policy of working hours that employees can choose a couple of different time options depending on their personal situations. And it turns out to be very effective to work & life balance, increasing organizational vitality, improving efficiency of business and productivity, recruitment of core human resources, and prevention of deviation; both team and personal performance levels has significantly improved. Therefore, it is highly recommended to adapt flexible working policies for companies seeking for a long term, sustainable corporate vision.