Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.291-291
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- 2004
Culture of Karyoplast and Cytoplast Complexes in High Osmolarity after Fusion Improve In Vitro Development of Porcine Nuclear Transfer Embryos
- Im, Gi-Sun (National Livestock Research Institute, RDA) ;
- Hwang, In-Sun (National Livestock Research Institute, RD) ;
- Kim, Dong-Hoon (National Livestock Research Institute, RD) ;
- Yang, Byoung-Chul (National Livestock Research Institute, RD) ;
- Kim, Se-Woong (National Livestock Research Institute, RD) ;
- Park, Hyo-Suk (National Livestock Research Institute, RD) ;
- Seo, Jin-Sung (National Livestock Research Institute, RD) ;
- Yang, Bo-Suk (National Livestock Research Institute, RD) ;
- Chang, Won-Kyong (National Livestock Research Institute, RDA)
- Published : 2004.06.01
Abstract
Micromanipulation and fusion are essential to generate nuclear transfer embryos. In this process cytoplasmic damage is unavoidable. This study investigated the hypothesis that higher osmolarity than normal culture medium could help oocytes recover from cytoplasmic damage from micromanipulation and electric pulse. Oocytes derived from a local slaughter house were matured for 42 ∼ 44 h and enucleated. (omitted)