Journal of Microbiology and Biotechnology

  • 제17권5호
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  • Pages.739-744
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  • 2007
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Production of Recombinant Polyhedra Containing Cry1Ac Fusion Protein in Insect Cell Lines

  • Kim, Jae-Su (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Choi, Jae-Young (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Roh, Jong-Yul (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Lee, Han-Young (Agricultural Technology Research Institute, Dongbu Hannong Chemical Co. Ltd.) ;
  • Jang, Seung-Sik (Agricultural Technology Research Institute, Dongbu Hannong Chemical Co. Ltd.) ;
  • Je, Yeon-Ho (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University)
  • 발행 : 2007.05.31
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초록

Insect cell lines and the control of infection for obtaining the maximum amount of polyhedrin-Cry1Ac-polyhedrin fusion protein from Bactrus in monolayer and suspension culture systems were tested. Growth rates of the Trichoplusia ni(High-Five) cell line in both culture systems were better than the other insect cell lines, Spodoptera frugiferda(Sf-9, Sf-21), Trichoplusia ni(Tn5), and Spodoptera exigua(Se301). The expression of the fusion protein in a monolayer culture showed that Se301 cells were 2.3-4.8 times more productive on a per cell basis than the other cell lines. However, in suspension culture, only High-Five cells were productive. High-Five cells infected with Bactrus at a multiplicity of infection(MOI) of 5 and a cell density of $3.0{\times}10^5$ cells per ml were more productive than the other infection condition in a suspension culture suitable for a large-scale production of baculovirus. In conclusion, for the large-scale production of Bactrus in vitro, High-Five cells showing good growth and high productivity are suitable.

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