• KSBB Journal
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• v.21 no.6 s.101
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• pp.422-427
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• 2006

The culture media from Fomitopsis pinicola were extracted by methanol and examined growth inhibition against Helicobacter pylori. The culture media from 8 days fermentation of F. pinicola showed maximum inhibition activity on H. pylori in 0.25 mg as MIC value. The highest activity against H. pylori by MHCS agar diffusion medium by Fp-P1 in 22.7 mm ID among 3 peaks from methanol fraction of 8 days culture media of Fomitopsis pinicola which purified by ion-exchange chromatography. The Fp-P1 from DEAE-Sephadex A-25 have been analysed by TLC as Fp-T1, Fp-T2 and Fp-T3 by ninhydrin staining. Fp-T3 (Rf value : 0.67) was higher activity against H. pylori in 14.4 mm ID. Fp-T3 was identified as single band by HPLC and TLC. It was assumed to aminosugar by BioLC analysis and TLC staining.

• Food Science and Biotechnology
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• v.17 no.1
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• pp.191-194
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• 2008

The antagonistic activity of probiotic strains (Bifidobacterium animalis BB-12, Bifidobacterium bifidum A, Bifidobacterium longum B6, Lactobacillus acidophilus ADH, Lactobacillus paracasei ATCC 25598, and Lactobacillus rhamnosus GG) against nalidixic acid resistant ($NA^R$) Escherichia coli O157:H7 MF1847, E. coli O157:H7 H2439, E. coli O157:H7 ATCC 43894, and E. coli O157:H7 C7927 was investigated using the agar-overlay, well diffusion, and broth culture tests. L. paracasei ATCC 25598 was the most effective probiotic strain in terms of in vitro antagonistic activity against $NA^R$ E. coli O157:H7, followed by L. rhamnosus GG, B. longum B6, and L. acidophilus ADH. The use of selected probiotic strains could be an effective pre-harvest intervention strategy to reduce the risk of $NA^R$ E. coli O157:H7 by maintaining a balanced microflora in animals and might provide many potential benefits in lieu of using antimicrobials.

• Journal of the Korea Society of Computer and Information
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• v.21 no.5
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• pp.135-139
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• 2016

Scrophularia ningpoensis hemsl has been traditionally used in China and Vietnam for treatment of bacteria, atopy, pimple, tonsillitis, angina and encephalitis for a long time. The main objectives of this study were to evaluate the antibacterial activity of the Scrophularia ningpoensis hemsl extract on biofilm formation of Klebsiella pneumoniae. Antibacterial activity was conducted using disc diffusion assay and minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined using the broth micro dilution method in accordance to Clinical and Laboratory Standards Institute guidelines(CLSI). Furthermore, cytotoxicity on L929 were assessed using animal cell culture for the proliferation test(MTT cell assay) and the biofilm forming capacity of the K. pneumoniae were determined using the colony forming unit (CFU) assay. The extract exhibited considerable antibacterial activity. K. pneumoniae was susceptible to the extract with the MIC and MBC of 0.1875 and $1.5mg/m{\ell}$ respectively. Cytoxicity test in L929 showed no sign of toxicity at the concentration of $0.75mg/m{\ell}$ and at the same concentration the extract caused inhibition of bacterial biofilm formation. The extract of Scrophularia ningpoensis hemsl possesses an in vitro antibacterial antibiofilm activities against K. pneumoniae, with no sign of cytoxicity on L929.

• KIEAE Journal
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• v.8 no.6
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• pp.71-78
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• 2008

Necessity of paradigm change has been awared in urban redevelopment especially, in housing regeneration from uniform scratch without serious consideration about resident's lives to holistic approach based on local culture and residents' participation. The purpose of this research was to identify community places recognized and attached by residents of a housing improvement district which was scheduled to be changed for improvement of the area, in near future. This research itself was considered as an resident participation technique. Group interview at a field site was carried as the main research methodology. As results, valuable places cherished by residents were identified. Most of them could be explained in relation to religious leadership, traditional cultural heritage and democratic movement. In regeneration process those places which have a strong tie with residents needs to be deliberately considered as a target to be preserved rather than demolished. The places also play a role in diffusing a livable village making movement. The result is expected to be used to make a cognitive and emotional community map of residents which is meaningfully differentiated from just a physical and geographical map. The places, place recognition activities and diffusion movement for common appreciation can be a basic foundation for residents participation in improving their housing neighborhood environment, and in creating value added strategies for the area.

• Journal of the Korean Solar Energy Society
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• v.26 no.3
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• pp.89-97
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• 2006

Our Housing culture continuously changes for the correspondence to social development and changes of economy, and be developing. A massive apartment complex continuously increased, and According to this in our country heating energy consumption of a residential building is continuously increasing at country me whom was limited in order to raise efficient residential land use and diffusion ratio of house. If confirm an element to be able to reduce use of a heating energy, and there is saving possibility to parts of energy saving, this study will present the standard amount used with bases to the gas amount used for heating and Field Test data about room temperature. Execute simulation with building balance (public area, a plain), the gas amount useds such as incense of a building, indoor setting temperature ($^{\circ}C$), a position of an apartment house etc. to affect the energy amount used of a valuation object building of a variable through Field Test and research on the actual condition. Calculate the standard amount used of city gas for winter season heating of a comparative analysis apartment house to data value getting the above results through Field Test and simulations with bases.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.5
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• pp.432-443
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• 2005

Microarray technology has contributed Significantly to the understanding of bacterial genetics and transcriptional regulation. One neglected aspect of this technology has been optimization of microarray-generated signals and quality of generated information. Full genome microarrays were developed for Clostridium acetobutylicum through spotting of PCR products that were designed with minimal homology with all other genes within the genome. Using statistical analyses it is demonstrated that Signal quality is significantly improved by increasing the hybridization volume. possibly increasing the effective number of transcripts available to bind to a given spot, while changes in labeled probe amounts were found to be less sensitive to improving signal quality. In addition to Q-RT-PCR, array validation was tested by examining the transcriptional program of a mutant (M5) strain lacking the pSOL1 178-gene megaplasmid relative to the wildtype (WT) strain. Under optimal conditions, it is demonstrated that the fraction of false positive genes is 1% when considering differentially expressed genes and 7% when considering all genes with signal above background. To enhance genomic-scale understanding of organismal physiology, using data from these microarrays we estimated that $40{\sim}55%$ of the C. acetobutylicum genome is expressed at any time during batch culture, similar to estimates made for Bacillus subtilis.

• Journal of the Korean Society of Costume
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• v.50
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• pp.183-196
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• 2000

Andy Warhol is one of the great artists in the modern history of art who compares favorably with Picasso. His activities had his object on pervasion of a pop art and profit of the art industry. Many previous studies on his activities have attracted and held the attention of his works of art after 1960s. However in spite of his working as publisher of a fashion magazine fashion illustrator fashion model displayer and etc. in 1950s. there are few studies on his activities of 1950s. Hence this study investigates his works of art in 1950s. and intensively analyzes his effect on modern fashion. In 1950s, Andy Warhol had already been distinguished in works of art from other commercial designers through the illustration for advertising design window display of stores and usage of Hollywood stars as anessential element in his works for whom he had sighed when he was young His works were also characterized by a rejection of they originality of commercial arts. He had an great effect on youth's fashion in 1950s and has activities in 1950s had influence on the diffusion of the public punk fashion in 1970s and the street fashion in 1980s.

• Journal of Technology Innovation
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• v.12 no.1
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• pp.161-188
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• 2004

The objective of this research is to evaluate the Creative Research Initiative Program (CRI), a national R＆D program funded by the Ministry of Science ＆ Technology in Korea. The evaluation of CRI covers the following research questions; 1) Have it set a unique position and characteristic distinguished from other government-funded R＆D programs\ulcorner 2) Are the achievements of the program relevant to its goal\ulcorner 3) What is its performances and how much is it achieved its goal\ulcorner The results are the followings; 1) CRI is perceived as a pure basic research, distinguished from other national basic research programs, such as the Coal Oriented Basic Research Program and the SRC and ERC. 2) CRI is a well-adapted R＆D program in confront of the environmental changes and R＆D needs, as well as follows the planned R＆D areas. 3) CRI have performed well in the raising-up world-class research leaders and the nation-wide diffusion of creative R＆D culture, while it got few performances in the overcoming the limitation of the existing technologies and the independent development of original key technologies for future industries. However, the duration of the program, 5 year, is too short to expect concrete outcome, such as creating original technologies. Many of the outcomes of CRI gets a lot of attention from top class scientists in the world, it is expected to generate various R＆D performances in the future.

• Journal of Sport and Applied Science
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• v.4 no.2
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• pp.11-17
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• 2020

Purpose: Sports is an activity created by human beings and is related with natural and human environments, and it is a physical activity which inherently interacts with geographical properties. With the above views, it is clear that sports reflect human culture and physical environment, which are closely related to places or spaces in terms of origin, propagation and diffusion, or geography in a broader sense. This study explored the relationships between sporting activities and geography with an environmental view. Research design, data, and methodology: The study employed qualitative methodology to review the association between sport and geographical perspective. The study reviewed the broad documents including journals, periodicals, reports and so forth. Results: First, since its inception in the 1960s, research on sports geography has been steadily studied in many international geography and non-geographic research journals. Second, it reflects the distinct characteristics of the relationship between sports and geography (relationship with physical geography and humanities). Third, as a spatial approach to sports, it is the place of sports, globalization of sports, and the tourist attraction of sports. Conclusion: the findings of this study provides insights for sport geography and could be applied to the current sports industry. Further implications were discussed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1228-1236
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• 1997

Lactobacillus strains were isolated from volunteer's feces, including from newly-born infants and adults in their 20's, by using differential MRS-BPB plates. Total 56 presumptive Lactobacillus strains were isolated and the bacteriocin productions by the isolates were examined by agar diffusion method. Six bacteriocin-producing strains were confirmed. Among them, two isolates, HU-1 and H22-3, showed the most outstanding antimicrobial activities, which were not affected by pH adjustments or catalase treatments of culture. HU-1 was originated from a two-years old boy and H22-3 was originated from a newly-born infant. HU-1 and H22-3 had the same morphology(short rod) when examined by scanning electron microscope, and the same biochemical traits including growth temperature range, salt tolerance and sugar-fermenting abilities. But the growth-inhibition spectrum and plasmid profiles of HU-1 and H22-3 were different. Both strains inhibited the growth of various Gram (+) microorganisms including Listeria monocytogenes. Micrococcus luteus, and Staphylococcus aureus in addition to many species of lactic acid bacteria, indicating the production of broad-spectrum bacteriocins. Bacteriocins produced by HU-1 and H22-3 were stable up to 90℃, 15 min heat treatments. Their activities were not affected by pepsin or trypsin treatments but destroyed by proteinaseK or pronase treatments.