• 제목/요약/키워드: Culture Complex

검색결과 959건 처리시간 0.024초

양어수경 복합시설에 수로형 사육시설의 적용 (Application of Channel Type Aquaculture System to the Complex Facilities with Tilapia Rearing and Hydroponics)

  • 이병일;이준구;홍상근;홍석우;배용수;김기덕
    • 생물환경조절학회지
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    • 제8권3호
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    • pp.209-215
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    • 1999
  • 수로형의 양어시설에서 틸라피아 사육시 성장도 및 사육수의 수질을 파악하여 수경재배시설에 수로형 사육시설의 병용 가능성을 조사하고, 펜타이트 파이프를 이용한 간이 수로형사육시설을 개발하고자 시험을 수행하였다. 폭 2m, 높이 1m,길이 19m의 수로형 사육시설 위에 수경 베드를 설치하고 틸라피아를 양식하였다. 수질은 사육하는데 이상 없이 유지되었고, 수로형 시설에서의 틸라피아의 성장은 원형사육시설에서의 그 것과 대등하였다. 따라서 수경재배시설에 수용할 수 있는 양어시설은 수로형이 적합하며 사육환경이 정상적이므로 수로형 사육시설의 도입이 가능하다고 판단되었다. 또한 양어수경 복합영농어시설은 높이 1 m, 폭 1.4 m, 길이 20 m의 크기로, 기둥은 25mm의 펜타이트 파이프와 사육조는 5m의 PP판넬을 사용하도록 하였으며, biofilter로는 모래를 10cm 정도 채워 작물을 재배하도록 하는 간이수로형 양어수경 복합시설의 구조를 고안하였다.

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배양중 흰쥐 간세포의 새포소기관 표지효소의 변천 (Transition of Marker Enzymes of Rat Hepatocyte Organelles in Culture)

  • 송인환;김주영;성인기;이융창
    • Journal of Yeungnam Medical Science
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    • 제6권2호
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    • pp.133-140
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    • 1989
  • 초기배양과정에서 일어나는 간세포의 회복, 성장, 활동상황등을 추적하기 위하여 세포의 생존과 기능에 꼭 필요하고 서로 깊은 연관관계를 가진 골지체, 사립체, 생체막의 표지효소를 일령별로 추적 하였다. 골지체의 표지효소인 thiamine pyrophosphatase는 배양 4일경부터 장기 배양시의 강도에 접근하였으며 사립체의 표지효소인 succinate dehydrogenase는 시일이 지나며 강도가 감소하여 4일부터는 일정한 강도를 유지하였다. 형질막의 표지효소인 alkaline phosphatase은 2일째부터 반응강도가 강해져 5일째 이후로는 아주 강한반응을 보였다. 이같은 결과를 종합해볼때 1차 배양에 있어 간세포는 세포 분리후 4일경이 지나면서 안정상태에 접어들고 제 기능을 수행하나 배양 상태에서는 분비세포로서의 고유기능은 감소되는 것으로 사료된다.

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국내 고층 주상복합 건물의 구조적 특성에 관한 조사 연구 (An Investigative Study on the Structural Characteristics of High-Rise Complexes in Korea)

  • 강석원;이성호
    • 한국건설관리학회논문집
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    • 제4권4호
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    • pp.137-144
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    • 2003
  • Recently, the interest in a complex building increases rapidly in Korea. High-rise complex is not simply the combination of a residential apartment and a business office as previous but a building with the object of the convenience of the residents familiar with city-life style through adapting the high class life style and new residential culture to the business space, and the efficiency in using the limited building site in the town. This study presents an exploratory analysis focusing the structural system with the help of the extensive survey of the construction site in Korea, and aims a guideline for the structural design and construction of high rise complex. Through the survey, change and development in the design and the construction can be seen as the height and size of the complex grow. It is almost indispensable to design a structural system against lateral forces like earthquake or wind, which is usually measured by story drift ratio or story displacement. Improvement of the structural materials and their usages is also included for the efficiency of the structural system. Useful slab-beam system contributing to the decrease of the story height is still a concern.

개성공단 분쟁해결 제도 정비방안 - 남북상사중재제도를 중심으로 (A Study on the Readjustment Plans for Solution of Conflict in Gaeseong Complex - Centering around the Inter-Korean Commercial Arbitration System)

  • 황보현
    • 한국중재학회지:중재연구
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    • 제29권4호
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    • pp.3-31
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    • 2019
  • In order to achieve full-scale economic cooperation between North Korea and South Korea, the Gaesong Industrial Complex should reopen first. In this case, the Inter-Korean commercial arbitration system should be clearly established to effectively resolve the disputes arising in the special economic zones of the Gaesong Industrial Complex. Even though the Inter-Korean Investment Security Agreement, the Agreement on the Resolution of Commercial Disputes between North Korea and South Korea, the Agreement on the Formation and Operation of the Inter-Korean Commercial Arbitration Committee, and the Agreement on the Formation and Operation of the Inter-Korean Commercial Arbitration Committee in the Gaesong Industrial District are in place, specific arbitration procedure is not concretely agreed upon and realized between the two Koreas. Therefore, the realization of commercial arbitration between them led by the Ministry of Unification or the government should be accomplished. In addition, it is necessary to consider the administrative trial or administrative litigation system in order to deal with administrative disputes that are not subject to commercial arbitration. Lastly, discussions on legal integration between the two Koreas should continue, focusing on the special economic zone of the Gaesong Industrial Complex, in order to prevent integration from being hindered by a different culture for a long time.

혼합미생물배양체를 이용한 폐활성슬러지 가용화 산물로부터 polyhydroxyalkanoate 생합성 (Biosynthesis of polyhydroxyalkanoate by mixed microbial cultures from hydrolysate of waste activated sludge)

  • 박태준;유영재;정동훈;이선희;이영하
    • 미생물학회지
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    • 제53권3호
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    • pp.200-207
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    • 2017
  • SA-TSA 복합구를 이용해 폐활성슬러지로부터 VFAs를 생산하고, 이를 발효 기질로 이용해 PHA를 생산하기 위한 실험을 수행하였다. 30%의 SA-TSA 복합구를 처리하였을 때 폐활성슬러지의 가용화 효율이 가장 효과적으로 일어나서 약 3,900 mg/L의 SCOD값을 얻었으며, acetic acid, propionic acid, iso-butyric acid 및 butyric acid를 주성분으로 하는 2,961 mg/L 농도의 VFAs를 얻었다. VFAs를 외부탄소원으로 이용하는 SBR 공정을 통해 PHA를 생합성하는 미생물을 농화배양하는 실험을 수행하고 PCR-DGGE로 분석한 결과, 우점 미생물로 Vibrio spp.와 Corynebacterium glutamicum이 확인되었다. 폐활성슬러지로부터 얻은 VFAs를 탄소원으로 사용하여 농화된 혼합미생물배양체를 회분배양한 결과, 건체량의 25.9%에 달하는 PHB를 얻었다. 본 연구결과는 SA-TSA 복합구를 이용하여 폐활성슬러지로부터 VFAs를 얻음으로써 폐슬러지 감량화 효과를 얻고, 또 혼합미생물배양체를 이용하여 VFAs를 바이오폴리머로 전환함으로써 경제성을 확보할 수 있는 새로운 생물공정의 가능성을 보여준다.

Effect of media compositions on carbohydrolase complex production in Lentinus edodes

  • 박점석;최민구;지영민;최정우;홍억기
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XII)
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    • pp.209-212
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    • 2003
  • 본 연구는 고수율의 아라비녹실란을 얻기위해 필수요소인 Lentinus edodes의 액체배양을 통해 생산되어지는 탄수화물 복합효소의 생산수율을 증대시키는데 그 목적이 있다. 그러기 위해 탄수화물 복합효소의 생산에 배지성분이 미치는 영향을 검토 하였다.

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Catechin Degradation by Several Fungal Strains Isolated from Mexican Desert

  • Aguilar Cristobal Noe;Mario Cruz;Raul Rodriguez;Gerardo Gutierrez-Sanchez;Ascencion Ramirez-Coronel;Christopher Augur
    • Journal of Microbiology and Biotechnology
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    • 제14권2호
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    • pp.426-429
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    • 2004
  • Eleven fungal strains previously isolated from the Mexican desert were evaluated for their capacity to use catechin as carbon source in submerged cultures. At 2 g/l of catechin, all strains grew better than the control strains, Aspergillus niger Aa-20. Aspergillus niger PSH and Penicillium commune EH2 degraded 79.33% and 76.35% with degradation rates of 0.0065 and 0.0074 g/l/h, respectively, when an initial catechin concentration of 3 g/l was used. Obtained results demonstrated the potential biotechnological capacity of these fungal strains to use condensed tannins as carbon source.

Comparisons of Physical Properties of Bacterial Celluloses Produced in Different Culture Conditions Using Saccharified Food Wastes

  • Moon Seung-Hyeon;Park Ji-Min;Chun Hwa-Youn;Kim Seong-Jun
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제11권1호
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    • pp.26-31
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    • 2006
  • The saccharogenic liquid (SFW) obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes was carried out by the cultivation supernatant of Trichoderma harziaum FJ1 culture. Acetobacter xylinum KJ1 was employed for the BC production culture. The physical properties, such as polymerization, crystallinity, Young's modulus, and tensile strength, of BCs produced by three culture methods: the static cultures using HS (Hestrin-Schramm) as a reference medium (A) or the SFW medium (B), the shaking culture (C) or the air circulation culture (D) using the SFW medium, were investigated. The degrees of polymerization of BCs produced under the different culture conditions (A-D) showed 11000, 9500, 8500, and 9200, respectively. Young's modulus was 4.15, 5.0, 4.0, and 4.6 GPa, respectively. Tensile strength was 124, 200, 80, and 184 MPa, respectively. All of the BC had a form of cellulose I representing pure cellulose. In the case of the shaking culture, the degree of crystallinity was 51.2%, the lowest degree. Under the other culturing conditions, the trend should remain in the range of 89.7-84%. Overall, the physical properties of BC produced from SFW were similar to those of BC from HS medium, a commercial complex medium, and BC production by the air circulation culture mode brought more favorable results in terms of the physical properties and its ease of scale-up. Therefore, it is expected that a new BC production method, like air circulation culture using SFW, would contribute greatly to BC-related manufacturing.

In Vitro Antagonistic Activity Evaluation of Lactic Acid Bacteria (LAB) Combined with Cellulase Enzyme Against Campylobacter jejuni Growth in Co-Culture

  • Dubois-Dauphin, Robin;Sabrina, Vandeplas;Isabelle, Didderen;Christopher, Marcq;Andre, Thewis;Philippe, Thonart
    • Journal of Microbiology and Biotechnology
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    • 제21권1호
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    • pp.62-70
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    • 2011
  • The antibacterial effects of nine lactic acid bacteria (LAB) against Campylobacter jejuni were investigated by using agar gel diffusion and co-culture assays. Some differences were recorded between the inhibition effects measured with these two methods. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, exhibited a clear anti- Campylobacter activity in co-culture assay with dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the supplementation of such medium with a cellulase A complex (Beldem S.A.) enhanced the antimicrobial effect of both LAB strains. The co-culture medium acidification and the C. jejuni were positively correlated with the cellulase A concentration. The antibacterial effect was characterized by the lactic acid production from the homofermentative E. faecium THT and the lactic and acetic acids production from the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties of LAB strains and enzyme combination could be used in strategies aiming at the reduction of Campylobacter prevalence in the poultry production chain and consequently the risk of human infection.

2002 한.일 국민교류년 기념' 한국과 일본의 신세대의 복식업화에 대한 연구 - Street Fashion을 중심으로 - (A Study on the Clothing Culture for New Generation of Korea and Japan)

  • 백샘이;간호섭
    • 복식
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    • 제53권3호
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    • pp.31-49
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    • 2003
  • The Purpose of this research is to examine and contrast the cultural characteristics of the street fashion in new generations of Korea and Japan. We also intended to help clothing companies understand the current trend in this new generation. As the method for street fashion analysis. various literature, internet information and new media materials were utilized. Key observations obtained are as following First, they pursue a complex image. New generations seem to admix images from different areas to create a synthetic image, rather than adopting a single image; Second, practicality is an important factor. They Pursue a sense of their own unique trend: Third, distinction. The difference in society values between Koreans and Japanese is incorporated. The Korean new generation considers the traditional moral principles as important social values. In contrast the Japanese new generation is greatly influenced by the open sexuality culture ; Fourth. public ownership and sharing of the culture. There is a gradual deviation from a dominating unidirectional influence from one country on another. Now the two countries are influencing each other ; Fifth, sense. There are numerous fashion items and generalized total coordination. New generations express more active and creative fashion sense in those fashion items.