• 한국지역지리학회지
• /
• 제3권2호
• /
• pp.37-87
• /
• 1997

만경강유역을 하나의 연장선상에 놓고 연구한 결과, 그 공간상에서 역사와 더불어 형성발달해온 시공연속체를 확인할 수 있었다. 만경강상류에서 하류 하구연안에 이르는 면장공간상에서의 개간과정은 여말에서 부터 시작되어 오늘에 이른 것으로 볼 수 있다. [기원지-지향지] 지향가설에서 본 개간과정에서 개간의 기원지는 만경강상류 산간계곡의 지류곡지 개간을 효시로 하여 기원지가 이루어지고, 조선조 중기까지는 수방대책의 발달과 더불어 하천 중류까지 진출하고, 하천 본류에 대한 하류지역의 계간은 하천의 규모와 유수량의 증가로 인한 하안의 홍수와 범람을 극복할 수 있는 인공제방을 축조할 수 있는 기술수준에 이른 1920년대에 들어서야 본격화되고, 그후 연이어 하구연안의 간석지 개간도 시행되어 개간의 개척첨단이 이들 지향지인 해안간석지일대에 형성되는 것을 볼 수 있다. 시간의 흐름과 더불어 각 시기마다 공간의 변화도 수반되어 시공연속체가 발달하는 것을 볼 수 있다. 취락의 경우 개간과정에 따라 산간계곡 산록일대에서는 주변입지적 집촌, 하천중류와 하류에서는 중앙입지적 집촌, 하천하구 간석지에서는 중앙입지적 열촌형태가 우세하게 나타났다.

• Journal of Plant Biotechnology
• /
• 제2권1호
• /
• pp.43-49
• /
• 2000

An efficient and reliable Agrobacterium transformation procedure based on TDZ (thidiazuron)-induced organogenesis was established and applied to six Brazilian eggp1ant varieties. Optimum transgenic plants recovery was achieved upon the study of the following parameters affecting transformation efficiency, using F-100 variety as a model: i) explant source; ii) pre-culture period; iii) physical state of the pre-culture medium and iv) coculture conditions. The highest frequency of kanamycin-resistant calli derived from leaf explants (5%) was obtained without a pre-culture period and co-cultivation for 24 h in liquid medium followed by five days on solid RM (regeneration medium). For cotyledon explants, best results were achieved upon a pre-culture of 24 h in liquid RM and a co-cultivation period of 24 h in liquid RM followed by three days in solid RM, resulting in a transformation Sequency of 22.7%. Kanamycin-resistant organogenic calli were also obtained from cultivars Emb, Preta Comprida, Round nose Shaded, Campineira and Florida Market. The expression pattern of an epidermis-specific promoter was studied using transformants expressing a chimaeric construct comprised by the promoter Atgrp-5 transcriptionally fused to the coding region of the gus gene. The expression pattern was similar to that previously observed in tobacco and Arabidopsis thaliana, with preferential expression at the epidermis and the stem phloem. These results support the idea that the Atgrp-5 promoter can be used to drive defense genes in these tissues, which are sites of pathogen interaction and spread, in programs for the genetic improvement of eggplant.

• Journal of Microbiology and Biotechnology
• /
• 제18권4호
• /
• pp.711-717
• /
• 2008

To speed up the conversion of rice straw into feeds in a low-temperature region, a start culture used for ensiling rice straw at low temperature was selected by continuous enrichment cultivation. During the selection, the microbial source for enrichment was rice straw and soil from two places in Northeast China. Lab-scale rice straw fermentation at $10^{\circ}C$ verified, compared with the commercial inoculant, that the selected start culture lowered the pH of the fermented rice straw more rapidly and produced more lactic acid. The results from denatured gradient gel eletrophoresis showed that the selected start culture could colonize into the rice straw fermentation system. To analyze the composition of the culture, a 16S rRNA gene clone library was constructed. Sequencing results showed that the culture mainly consisted of two bacterial species. One (A) belonged to Lactobacillus and another (B) belonged to Leuconostoc. To make clear the roles of composition microbes in the fermented system, quantitative PCR was used. For species A, the DNA mass increased continuously until sixteen days of the fermentation, which occupied 65%. For species B, the DNA mass amounted to 5.5% at six days of the fermentation, which was the maximum relative value during the fermentation. To the authors' best knowledge, this is the first report on ensiling rice straw with a selected starter at low temperature and investigation of the fermented characteristics.

• 원예과학기술지
• /
• 제19권4호
• /
• pp.471-475
• /
• 2001

난지형 마늘 재배에 있어서 시비노력 절감과 품질향상을 위한 완효성 비료의 시용효과를 구명하기 위해 실시한 실험의 결과를 요약하면 다음과 같다. 먼저 생육은 관행시비구에 비하여 완효성 비료시비구가 양호하여 초장이 4-6cm 더 길었고 엽초경이 2mm 이상 더 두꺼웠으며 엽수도 많은 경향이었다. 엽록소 함량은 월동기를 지나면서 점차 감소하다가 3월 하순 이후에 증가하였으며 생육후기인 5월에 다시 감소하는 경향을 보였으며 비종별로는 관행시비구가 추비의 영향으로 엽록소 함량이 완효성 비료 시험구보다 높은 경향을 보였다. 완효성 비료 중 CDU100처리구는 엽장이 길고 엽폭이 넓었으며 식물체 전체 중량이 무거웠다. 특히, 대구율이 관행(35.0%)보다 10.9% 높았고, 수량도 관행(1,270kg/10a)보다 5% 증수되었다. 전질소 및 피루브산 함량은 질소 무비구 및 속효성 비료 시비구에 비하여 완효성 비료 시험구에서 높은 경향을 보였다. 또한 완효성 비료 시용은 10a당 3.5시간의 시비노력이 소요되어 관행인 속효성 비료의 시비시간 4.6시간보다 24%의 시비노력 절감을 가져왔다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권10호
• /
• pp.1390-1394
• /
• 2004

We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. Transformation efficiency was influenced by the A. tumefaciens strain, addition of the phenolic compound acetosyringone and duration of vacuum treatment. Of the three A. tumefaciens strains tested, EHA101/pIG121Hm was found to be most effective followed by GV3101/pIG121Hm and LBA4404/pIG121Hm for transient GUS expression after 3 days co-cultivation. Inclusion of 100 $\mu$M acetosyringone in both the inoculation and co-cultivation media lead to an improvement in transient GUS expression observed in targeted calli. Vacuum treatment during infection of calli with A. tumefaciens strains increased transformation efficiency. The highest stable transformation efficiency of transgenic plants was obtained when mature seed-derived calli infected with A. tumefaciens EHA101/pIG121Hm in the presence of 100 $\mu$M acetosyringone and vacuum treatment for 30 min. Southern blot analysis indicated integration of the transgene into the genome of tall fescue. The transformation system developed in this study would be useful for Agrobacterium-mediated genetic transformation of tall fescue plants with genes of agronomic importance.

• 인삼문화
• /
• 제2권
• /
• pp.9-16
• /
• 2020

우리나라 한방 의서 중 인삼의 첫 기록은 고려 시대 임시 수도인 강화 도읍기 때 강화도에서 출간된 향약구급방에 나타나 있다. 강화지역에서 인삼재배의 시작은 1100년경부터 시작되었을 것으로 사료 된다. 본격적인 인삼재배 시작은 1920년경 개성인삼조합의 특별구역으로 지정되면서부터였고, 1950년 한국전쟁 시작 때까지 계속되었다. 1953년 휴전 이후부터 강화지역에서 인삼재배가 다시 시작되었다. 1967년에 강화삼업조합이 창립되었으며, 이때 재배 면적이 약 200ha(60만평)이었고, 1974년에 약 900ha(270만평)으로 증가 되었다. 따라서 강화는 1970년대 중반기에 우리나라 전국 시, 군 가운데 인삼재배 면적과 생산량이 가장 높은 지역이였다. 강화지역에서 생산되는 인삼은 주로 6년근이고, 홍삼원료로 사용되어 국내 인삼생산지로 더욱 유명하게 되었고, 그 명성이 현재도 계속되고 있다.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.131.2-132
• /
• 2003

White rot on garlic caused by Sclerotium cepivorum firstly occurred at Goheoung, Jeonnam in 1998. Thereafter, the disease rapidly spread throughout the country except Gangwon and became a major limiting factor for the cultivation of various Allium species such as garlic, onion, and welsh onion. The disease that has not been reported on a wild garlic(Allium monanthum) previously occurred severely at Seosan, Choongnam in 2003. Among cultivation areas in the region, 10.7％ were infected by the disease and the ratio of diseased plant reached up to 55.0％ in some heavily infected fields. Two species of Sclerotium were consistently isolated from infected samples and identified as S. cepivorum or another Sclerotium sp. Averaged size of sclerotium of the former was 455.0x562.2 urn, while the later was 374.4${\times}$347.2$\mu\textrm{m}$. Patogenicity to Allium species and mycological characteristics such as sclerotium size, growth temperature, and microconidia of the fungi were similar to those reported on other Allium species previously. Consequently, the wild garlic is a newly reported host of the two pathogenic fungi in Korea.

• 생물환경조절학회지
• /
• 제25권2호
• /
• pp.95-99
• /
• 2016

PO 필름 공급이 경북 성주지역 참외 산업에 미치는 영향을 분석하기 위하여 참외 재배면적, 재배 농가수, 수량 및 소득 등을 분석한 결과, PE 필름은 2010년에 비해 2014년도에는 전국, 경북 성주 지역에서 각각 91.5%, 94.9%, 95.2%로 감소한 반면, PO 필름 보급은 각각 342.5%, 500%, 825% 이었다. 2010년에 비해 2015년 재배면적과 농가수는 각각 8.8%, 11.9% 감소하였다. 수량과 생산량은 각각 38.2%, 25.9% 증가하였다. 조수입은 2010년 3,051 억원에서 2015년 4,020 억원으로 1.3배 증가하였고 소득도 2010년 1,936 억원에서 2015년 4,020 억원으로 2.7배 증가하였다. 이상의 결과를 종합 하면 PO 필름으로 교체되면서, 참외 생산성은 증대되고, 작업 노력 및 유해물질 배출은 감소될 것으로 보여진다.

• 한국토양비료학회지
• /
• 제47권6호
• /
• pp.500-505
• /
• 2014

In recent years, organic ginseng cultivation has increased because customers prefer organic ginseng products due to the morphological quality as well as the safety such as the residuals of chemically-synthesized pesticides. Therefore, some of paddy and upland fields were converted into organic ginseng fields. Soil chemical properties, soil microflora, and soil-inhabiting animals were investigated in paddy-converted and upland organic ginseng fields in Sangju city, Korea. There was few difference in the soil chemical properties, and the soil nutrient concentrations, such as nitrate-N, Av. $P_2O_5$ between the two field types, and exchangeable cations such as K and Ca were within the ranges which are recommended by the standard ginseng-farming manual. Changes in microflora were also assessed by analyzing phospholipid fatty acid composition. Overall, indicators of microbial groups were greater in the upland field than in the paddy-converted soil, but they were not significantly different. In addition, there was no significant change in the abundance of nematodes, collembolans, and mites between the two field types probably because of the high variation within the field types. In this study, it was suggested that soil chemical and biological properties for organic ginseng cultivation were greatly influenced by the variation of topography and soil management practices rather than field types. Further study may be needed to investigate the influence of these factors on soil chemical and biological properties in organic ginseng soils.

• Journal of Microbiology and Biotechnology
• /
• 제17권1호
• /
• pp.81-88
• /
• 2007

The sprC gene encodes Streptomyces griseus protease C (SGPC), a bacterial chymotrypsin-like serine protease. Because the published data on sprC was not complete, we cloned and analyzed a new DNA fragment spanning downstream to upstream of the sprC gene from S. griseus IFO13350. The cloned 2.3-kb DNA fragment was placed on a high-copy number plasmid and introduced into Streptomyces lividans TK24. Chymotrypsin activity of the transformant was 8.5 times higher than that of the control after 3 days of cultivation and stably maintained until 9 days of cultivation, which dearly indicated that the cloned 2.3-kb fragment contained the entire sprC gene with its own promoter. When the same construct was introduced in the S. griseus IFO13350 (wild strain) and its two mutant strains in the A-factor regulatory cascade, ${\Delta}adpA$ and HO1, the chymotrypsin activity increased fivefold only in the ${\Delta}adpA$ strain. Transcriptional analysis based on RT-PCR revealed that the sprC gene is normally transcribed in both strains; however, earlier transcription was observed in the wild strain compared with the ${\Delta}adpA$ strain. A gel mobility shift assay showed that the AdpA protein did not bind to the promoter region of sprC. All these data clearly indicate that the expression of sprC is not dependent on the AdpA protein, but is distinctly regulated from other chymotrypsin genes composing an AdpA regulon. Earlier morphological differentiation was observed in S. lividans TK24, and S. griseus IFO13350 and HO1, transformed with the expression vector. The transformant of S. griseus ${\Delta}adpA$ formed markedly larger colonies. Antisense repression of sprC resulted in severe decrease of chymotrypsin activity, down to one-third of the control, and delayed morphological differentiation. All these data suggest that SGPC is related to normal morphogenesis in S. griseus.