• Journal of Microbiology
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• v.44 no.2
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• pp.155-161
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• 2006

Comparative analysis of microbial communities in a sequencing batch reactor which performed enhanced biological phosphorus removal (EBPR) was carried out using a cultivation-based technique and 16S rRNA gene clone libraries. A standard PCR protocol and a modified PCR protocol with low PCR cycle was applied to the two clone libraries of the 16S rRNA gene sequences obtained from EBPR sludge, respectively, and the resulting 424 clones were analyzed using restriction fragment length polymorphisms (RFLPs) on 16S rRNA gene inserts. Comparison of two clone libraries showed that the modified PCR protocol decreased the incidence of distinct fragment patterns from about 63 % (137 of 217) in the standard PCR method to about 34 % (70 of 207) under the modified protocol, suggesting that just a low level of PCR cycling (5 cycles after 15 cycles) can significantly reduce the formation of chimeric DNA in the final PCR products. Phylogenetic analysis of 81 groups with distinct RFLP patterns that were obtained using the modified PCR method revealed that the clones were affiliated with at least 11 phyla or classes of the domain Bacteria. However, the analyses of 327 colonies, which were grouped into just 41 distinct types by RFLP analysis, showed that they could be classified into five major bacterial lineages: ${\alpha},\;{\beta},\;{\gamma}-$ Proteobacteria, Actinobacteria, and the phylum Bacteroidetes, which indicated that the microbial community yielded from the cultivation-based method was still much simpler than that yielded from the PCR-based molecular method. In this study, the discrepancy observed between the communities obtained from PCR-based and cultivation-based methods seems to result from low culturabilities of bacteria or PCR bias even though modified culture and PCR methods were used. Therefore, continuous development of PCR protocol and cultivation techniques is needed to reduce this discrepancy.

• The Korean Journal of Mycology
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• v.47 no.3
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• pp.233-240
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• 2019

This study was performed to develop a useful method for substrate pH adjustment in bottle cultivation of Grifola frondosa. Four types of organic acid solution-citric acid, malic acid, tartaric acid, and succinic acid-were added at 0.5% to 3.0% (w/v) into the substrate. The pH after the organic acid treatments ranged from 3.8 to 4.8 for the mycelial growth of G. frondosa after sterilization. Supplementation with succinic acid at 0.5% resulted in a shorter cultivation period and higher consistent yields compared to other organic acid treatments. These results indicate that the technique we developed is beneficial for higher production of G. frondosa using bottle cultivation.

• Journal of Mushroom
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• v.12 no.4
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• pp.263-269
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• 2014

Although sawdust cultivation of shiitake (Lentinula edodes) is becoming more common, it is insufficiently competitive in spring and autumn, the best time to breed shiitake. Thus, it is urgently needed to develop a technique for all year round cultivation of shiitake using mushroom growing beds. In the present study, the temperature changes according to the location of shiitake cultivation facilities were investigated. We confirmed that a refrigerator, an air conditioner, triple membranes, shiitake cultivation beds, fog nozzles which were installed in the shiitake cultivation facilities play an important role in keeping the low temperature. Bag cultivation of shiitake was tested in temperature variation from $14^{\circ}C$ to $29^{\circ}C$ with a $3^{\circ}C$ interval to know its cultivating temperature range in hot summer season. In summary, the sawdust cultivation of shiitake is possible when the temperature difference between top and bottom is maintained below $1^{\circ}C$. And the temperature of the shiitake cultivation facilities should be maintained below $23^{\circ}C$ in the induction period for fruitbody formation.

• Korean Journal of Remote Sensing
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• v.37 no.5_1
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• pp.1199-1206
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• 2021

Accordingly, attention is also being paid to the agricultural use of remote sensing technique that non-destructively and continuously detects the growth and physiological status of crops. However, when remote sensing techniques are used for crop monitoring, it is possible to continuously monitor the abnormality of crops in real time. For this, standard growth information of crops is required and relative growth considering the cultivation environment must be identified. With the relationship between GDD (Growing Degree Days), which is the cumulative temperature related to crop growth obtained from ideal cultivation management, and the vegetation index as standard growth information, compared with the vegetation index observed with the spectralreflectance sensor(SRS_NDVI & SRS_PRI) in each rice paddy treated with standard cultivation management and non-fertilized, it was quantitatively identified as a time series. In the future, it is necessary to accumulate a database targeting various climatic conditions and varieties in the standard cultivation management area to establish a more reliable standard growth information.

• Journal of Mushroom
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• v.5 no.2
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• pp.51-58
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• 2007

These experiment was conducted to find the superior strain selection, cultivation technique and optimum environmental condition of nameko mushroom culture using plastic container. The results was following as Mycelium of Pholiota nameko grown well at MCM and Hamada media, and its media acidity was pH 6~7. The optimum temperature condition for growing mycelium was $25^{\circ}C$. Under $15^{\circ}C$ and above $30^{\circ}C$ of temperature condition, mycelium growing speed was delayed remarkably. Among the 29 strains of nameko mushroom, the most productive strains was JNM19007, JNM19026, JNM19027 and JNM19028. The optimum media composition rate for produce fruitbody was pine sawdust 80% + wheat bran 20%. In this condition, the average fruitbody amount was 188g per 1,100cc container. The optimum post-culturing period was 50 days and mushroom sprout appeared 7 days after old mycelia removed. The suitable temperature was $12^{\circ}C$ for induce sprout, growing period was $16^{\circ}C$ and the optimum relative humidity was 95% in all culturing periods.

• Research in Plant Disease
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• v.21 no.3
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• pp.243-249
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• 2015

Soybean frogeye leaf spot caused by the fungus Cercospora sojina Hara, has known to lead a severe reduction of crop yield. Since frogeye leaf spot on soybean has recently become a serious problem in Korea, the susceptibility of recent recommended cultivars against C. sojina had been tested. To standardize the disease severity of soybean, the optimum sporulation condition of C. sojina and the disease index were established in this study. Sporulation was maximized on the 10% V8 juice agar with 12 h light and 12 h dark at $25^{\circ}C$. Spore suspension ($10^5spores/ml$) was sprayed on the leaves of soybean (V6 stage), and the disease responses to each isolate were evaluated on 28 days after inoculation. As a result, Daepung, Shinpaldal2ho, Yeonpung and Cheonga showed the resistance reaction to 8, 7, 6, 6 isolates of C. sojina, respectively, whereas Cheongja, Hwangkeum, Taekwang, Daewon, Cheonsang and Sinhwa showed the susceptible reaction to 8 isolates of C. sojina. Breeding the resistant soybean cultivars against C. sojina requires a uniform resistance for screening technique. The disease index of frogeye leaf spot on soybean developed in this study can be effectively used for the accurate field assay to select the frogeye leaf spot resistant soybean.

• Korean Journal of Organic Agriculture
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• v.24 no.3
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• pp.385-412
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• 2016

This paper analyzes economic efficiency of strawberries and tomatoes farms using the data envelopment analysis (DEA). We examine how production characteristics and farm size affect overall efficiency (OE), allocative efficiency (AE), and technological efficiency (TE). We also survey sample farm households from the Farm Income Investigation database of Rural Development Administration to investigate management and cultivation techniques and to analyze economic efficiency by technique. Implications for improving efficiency are suggested in the conclusion.

• The Korean Journal of Community Living Science
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• v.18 no.2
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• pp.247-263
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• 2007

This study was complied to analyze the professional agricultural techniques level and to suggest a desirable direction for the professional education system for women farmer. The research was conducted by using in-field surveys and interviews. Data was gathered by questionnaire from 147 women farmer in five specialized crop regions: strawberry, cucumber, rose, apple, and oyster mushroom. The results obtained are as follows. First, the technique levels in computer usage and electronic commerce, machinery usage, pesticide and fertilizer utilization, facility automation, and eco-agricultural cultivation were low. Second, the demand for professional education, evaluated through technique level by standard management diagnosis, was recognized. Areas of concern included: cucumber (temperature control, carbonic acid gas control, grading, funds management), strawberry (light control, soil temperature control, irrigation watering, shipping), rose (temperature control, light control, funds management, cooperated management), oyster mushroom (growth cabinet sterilizer), and apple (flower bud pinching, defloration, fruit thinning, funds management). Based on the results of this study, the following are suggestions for the planning of a professional education system for women farmer. First, it needs to address formal education in marketing, machinery usage, facilities automation, and techniques in pesticide and fertilizer utilization. Second, it needs to be a multi- level program with appropriate terminology at every level which is suitable to each age and ability of women farmer. Third, it needs a more comprehensive manual developed by need analysis of women farmer and a larger lecturer pool for professional education.

• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.96-102
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• 1998

Primary hepatocytes of small animals such as rat and rabbit were often used for the study of extracorporeal liver support systems. Freshly isolated rat hepatocytes form spheroids within tow days when cultivated as suspension in spinner vessels. These spheroids showed enhanced liver specific functions and more differentiated morphology compared to hepatocytes cultured as monolayers However, shear stress caused by continuous agitation deteriorated spheroids gradually. In this work we immobilized spheroids to prolong liver specific activities. First, hepatocyte spheroids were suspended in collagen solution containing calcium chloride and then dropped into alginate solution. A thin layer of calcium alginate was formed around the droplet and then was removed after the inner collagen was gelled by treatment of sodium citrate buffer. Spheroids embedded in collagen-gel bead maintained liver specific functions such as albumin secretion rate longer than hepatocyte spheroids exposed to shear stress. Therefore, we suggest that this immobilization technique may offer an effective long-term hepatocyte cultivation and facilitase the development of a bioartificial liver support device.

• Smart Media Journal
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• v.6 no.4
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• pp.58-64
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• 2017

Modern society is characterized by rapid increase in world population, aging of the rural population, decrease of cultivation area due to industrialization. The food problem is becoming an important issue with the farmers and becomes rural. Recently, the researches about the field of the smart farm are actively carried out to increase the profit of the rural area. The existing smart farm researches mainly monitor the cultivation environment of the crops in the greenhouse, another way like in the case of poor quality t is being studied that the system to control cultivation environmental factors is automatically activated to keep the cultivation environment of crops in optimum conditions. The researches focus on the crops cultivated indoors, and there are not many studies applied to the cultivation environment of crops grown outside. In this paper, we propose a method to improve the harvestability of poor areas by monitoring the areas with bad harvests by using big data analysis, by precisely predicting the harvest timing of fruit trees growing in orchards. Factors besides for harvesting include fruit color information and fruit weight information We suggest that a harvest correlation factor data collected in real time. It is analyzed using the Apache Spark engine. The Apache Spark engine has excellent performance in real-time data analysis as well as high capacity batch data analysis. User device receiving service supports PC user and smartphone users. A sensing data receiving device purpose Arduino, because it requires only simple processing to receive a sensed data and transmit it to the server. It regulates a harvest time of fruit which produces a good quality fruit, it is needful to determine a poor harvest area or concentrate a bad area. In this paper, we also present an architectural model to determine the bad areas of fruit harvest using strong data analysis.