Analysis of Microbial Communities Using Culture-dependent and Culture-independent Approaches in an Anaerobic/Aerobic SBR Reactor
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Lu Shipeng
(Division of Environmental Biotechnology & PMBBRC)
Park Min-Jeong (Division of Environmental Biotechnology & PMBBRC) Ro Hyeon-Su (Division of Environmental Biotechnology & PMBBRC, Department of Microbiology & Research Institute of Life Science, Gyeongsang National University) Lee Dae-Sung (School of Environmental Science & Engineering, POSTECH) Park Woo-Jun (Division of Environmental Biotechnology & PMBBRC, Division of Environmental Science and Ecological Engineering, Korea University) Jeon Che-Ok (Division of Environmental Biotechnology & PMBBRC) |
1 | Lim, Y.W., S.A. Lee, S.B. Kim, H.Y. Yong, S.H. Yeon, Y.K. Park, D.W. Jeong, J.S. Park. 2005. Diversity of denitrifying bacteria isolated from Daejeon sewage treatment plant. J. Microbiol. 15, 383-390 과학기술학회마을 |
2 | Davis, K.E., S.J. Joseph, and P.H. Janssen. 2005. Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl. Environ. Microbiol. 71, 826-834 DOI ScienceOn |
3 | Acinas, S.G., V. Klepac-Ceraj, D.E. Hunt, C. Pharino, I. Ceraj, D.L. Distel, and M.F. Polz. 2004. Fine-scale phylogenetic architecture of a complex bacterial community. Nature 430, 551-554 DOI ScienceOn |
4 | Jeon, C.O., D.S. Lee, and J.M. Park. 2001. Enhanced biological phosphorus removal in an anaerobic/aerobic sequencing batch reactor: Characteristics of carbon metabolism. Water Environ. Res. 73, 301-306 DOI ScienceOn |
5 | Janssen, P.H., P.S. Yates, B.E. Grinton, P.M. Taylor, and M. Sait. 2002. Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl. Environ. Microbiol. 68, 2391-2396 DOI |
6 | Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680 DOI |
7 | Kampfer, P., R. Erhart, C. Beimfohr, J. Bohringer, M. Wagner, and R. Amann. 1996. Characterization of bacterial communities from activated sludge: culture-dependent numerical identification verse in situ identification using group- and genus-specific rRNA-targeted oligonucleotide probes. Microb. Ecol. 32, 101-121 |
8 | Kong, Y., J.L. Nielsen, and P.H.Nielsen. 2004. Microautoradiographic study of Rhodocyclus-related polyphosphate-accumulating bacteria in full-scale enhanced biological phosphorus removal plants. Appl. Environ. Microbiol. 70, 5383-5390 DOI ScienceOn |
9 | Amann, R.I., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, 143-169 |
10 | Ellis, R.J., P. Morgan, A.J. Weightman, and J.C. Fry. 2003. Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil. Appl. Environ. Microbiol. 69, 1662-1669 DOI |
11 | Eschenhagen, M., M. Schuppler, and I. Roske. 2003. Molecular characterization of the microbial community structure in two activated sludge systems for the advanced treatment of domestic effluents. Water Res. 37, 3224-3232 DOI ScienceOn |
12 | Klepac-Ceraj, V., M. Bahr, B.C. Crump, A.P. Teske, J.E. Hobbie, and M.F. Polz. 2004. High overall diversity and dominance of microdiverse relationships in salt marsh sulphate-reducing bacteria. Environ. Microbiol. 6, 686-698 DOI ScienceOn |
13 | Mino, T., V. Arun, Y.T. Suzuki, and T. Matsuo. 1987. Effect of phosphorus accumulation on acetate metabolism in the biological phosphorus removal process, p. 27-38. In R. Ramadori (ed.). Biological Phosphate Removal from Wastewaters. Pergamon Press, Oxford, United Kingdom |
14 | Wang, G.C. and Y. Wang. 1997. Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes. Appl. Environ. Microbiol. 63, 4645-4650 |
15 | Felsenstein, J. 2002. PHYLIP (phylogeny inference package), version 3.6a, Seattle: Department of Genetics, University of Washington, Seattle, WA, USA |
16 | Jeon, C.O., W. Park, P. Padmanabhan, C. DeRito, J.R. Snape, and E.L. Madsen. 2003a. Discovery of a bacterium, with distinctive dioxygenase, that is responsible for in situ biodegradation in contaminated sediment. Proc. Natl. Acad. Sci. USA 100, 13591-13596 |
17 | Seviour, R.J., T. Mino, and M. Onuki. 2003. The microbiology of biological phosphorus removal in activated sludge systems. FEMS Microbiol. Rev. 27, 99-127 DOI ScienceOn |
18 | Zhou, J., M.A. Bruns, and J.M. Tiedje. 1996. DNA recovery from soils of diverse composition. Appl. Environ. Microbiol. 62, 316-322 |
19 | Jeon, C.O., D.S. Lee, and J.M. Park. 2003b. Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Water Res. 37, 2195-2205 DOI ScienceOn |
20 | Thompson, J.R., L.A. Marcelino, and M.F. Polz. 2002. Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'. Nucleic Acids Res. 30, 2083-2088 DOI ScienceOn |
21 | Jeon, C.O. and J.M. Park. 2000. Enhanced biological phosphorus removal in a sequencing batch reactor supplied with glucose as a sole carbon source. Water Res. 34, 3470-3480 |
22 | Briones, A. and L. Raskin. 2003. Diversity and dynamics of microbial communities in engineered environments and their implications for process stability. Curr. Opin. Biotechnol. 14, 270-276 DOI ScienceOn |
23 | Qiu, X., L. Wu, H. Huang, P.E. McDonel, A.V. Palumbo, J.M. Tiedje, and J. Zhou. 2001. Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning. Appl. Environ. Microbiol. 67, 880-887 DOI ScienceOn |
24 | Kim, J.B., M.S. Moon, D.H. Lee, S.T. Lee, M. Bazzicalupo, and C.K. Kim. 2004. Comparative analysis of cyanobacterial communities from polluted reservoirs in Korea. J. Microbiol. 14, 181-187 |
25 | Smolders, G.J.F., J. van der Meij, M.C.M. van Loosdrecht, and J.J. Heijnen. 1994. Model of the anaerobic metabolism of the biological phosphorus removal process: stoichiometry and pH influences. Biotechnol. Bioeng. 43, 461-470 DOI ScienceOn |
26 | Lane, D.J. 1991. 16S/23S rRNA Sequencing. In E. Stackebrandt and M. Goodfellow (ed.), Nucleic Acid Techniques in Bacterial Systematics. p. 115-147. John Wiley & Sons, New York |
27 | American public health association. Standard methods for the examination of water and wastewater, 19th ed. Washington, DC: American Public Health Association, 1995 |
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