• Proceedings of the KSRS Conference
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• 2002.10a
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• pp.757-765
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• 2002

This paper analyzes three theories for land degradation assessment and internationl/domestic methods for land degradation monitoring and assessment. Under the guidance of absolute degradation thought, this paper proposes the technological framework for monitoring and appraising cultivated land degradation based on the 3S technique. We can apply 3S technique and analyze the nature, the environmental, the social, and the economic elements which influence the land utilization and degradation synthetically, to set up the indicator system of the cultivated land degradation monitoring and assessment based on 3S technique; to propose the degradation information extraction methods based on 3S technique; to create the quantitative assessment model and method for land degradation; to analyze the ecological environment response of land use and degradation quantitatively; and to propose the measure, policy and suggestion for solving the land degradation problem from the point of view of land utilization.

• Journal of Ginseng Research
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• v.37 no.4
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• pp.491-495
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• 2013

Wood-cultivated ginseng, including roots in its dried form, is produced in forest land without using artificial facilities such as light barriers. To identify suitable sites for the propagation of wood-cultivated ginseng, factor combination technique (FCT) and linear combination technique (LCT) were used with geographic information system and the results were superimposed onto an actual wood-cultivated ginseng plantation. The LCT more extensively searched for suitable sites of cultivation than that by the FCT; further, the LCT probed wide areas considering the predominance of precipitous mountains in Korea. In addition, the LCT showed the much higher degree of overlap with the actual cultivation sites; therefore, the LCT more comprehensively reflects the cultivator's intention for site selection. On the other hand, the inclusion of additional factors for the selection of suitable cultivation sites and experts' opinions may enhance the effectiveness and accuracy of the LCT for site application.

• Journal of Plant Biology
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• v.39 no.3
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• pp.203-207
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• 1996

On the basis of karyotypic analysis performed by conventional staining and Giemas C-banding technique, cytological relationship was inferred for 21 lines of Paeonia lactiflora Pal. cultivated in Korea. It was very difficult to infer their organized karyotypic classification system using the composition of somatic chromosomes involving sat-chromosomes, relative length of chromosomes, arm ratio and karyotypic formulae by conventional staining. From the distribution and number of Giemsa C-bands on the chromosomes b and c, 21 lines can be subclassified into 5 groups. It seems that the karyotypic polymorphism is observed in 21 lines of cultivated P. lactiflora because peony mainly propagates by outbreeding.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.265-271
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• 2004

Genetic variation is the basis of crop improvement. Limited genetic diversity in a crop species may restrict the amount of genetic improvement that can be achieved through plant breeding. Soybean is one of the world's most important crops. A potential source of genetic variability for the cultivated soybean is the wild species G. soja Sieb. ＆amp; Zucc. Amplified fragment length polymorphism (AFLP) analysis is a PCR-based technique, which can detect a 10-fold greater nubmer of loci than other DNA marker analysis. Twenty cultivated soybeans and two-hundred wild soybeans were used to determine genetic vatiations by AFLPs and evaluate the usefulness of AFLPs as DNA markers. Six-hundred and ten fragments were detected with an average of 56 AFLP fragments produced per primer in a total of 11 AFLP primer pairs. The number of polymorphic loci detected per primer ranged from 7 to 20 and the polymorphism was greater in wild than in cultivated soybean. F$_2$ segregation analysis of four AFLP fragments in combination of Hwaeomputkong ${\times}$ PI 417479 indicated that they segregate as stable Mendelian loci with 3 : 1. This results strongly suggest that the AFLP analysis is a good technique for the detection of genetic polymorphism in a wide plant species.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.4
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• pp.239-246
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• 2003

We examined the relationships among community composition, microbial population, and microbial biomass to determine whether different land use leads to differences in microbial community composition. And also the relationships between soil characteristics and microbial community composition were investigated. There was no difference in pH between uncultivated and cultivated soils, but electrical conductivity, and contents of organic matter, available P and exchangeable cations were greater in the cultivated soil compared to the uncultivated soil. A linear correlation ($r^2=0.557$, n=18, p<0.01) was found between biomass-C estimated with fumigation extraction technique and total amount of fatty acids. An increase of fatty acid methyl esters (FAMEs) for bacteria, actinomycetes, fungi and protozoa was observed in cultivated soil.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.2
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• pp.95-102
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• 2023

This study was to investigate the cultivation technique previously conducted cultivation research for the stable production of alfalfa and to present further research. The data used in the study were 270 alfalfa cultivation experimental data from 1983 to 2008, indicating the cultivation region, field type, variety, sowing, cutting frequency, fertilization, and dry matter yield (DMY). The average DMY of alfalfa in the Republic of Korea was 12,536 kg/ha, which differed greatly depending on the cultivated region. Most of the field type was cultivated in upland. In order to increase alfalfa production, it is necessary to use reclaimed and unused land, and research on the soil amendment matter to improve the soil condition is needed. Alfalfa varieties cultivated an amount of 53, but collected data no studies considered fall dormancy, the criteria for selecting alfalfa varieties, so further research is required. The fertilizer did not consider each component at various levels, and research is needed as the demand for fertilizer. In particular, research on potassium is needed considering the increase in alfalfa production. The alfalfa cutting frequency differed in the estimated pasture production period depending on the region, and the DMY tended to increase with increasing cutting frequency. This suggests that the alfalfa DMY can be increased according to the cutting frequency in the Republic of Korea, so research is needed to present the appropriate cutting frequency.

• Fisheries and Aquatic Sciences
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• v.25 no.10
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• pp.525-536
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• 2022

Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scale-up production because it showed the highest specific growth rate (0.20 h^-1) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 10¹⁰ colony-forming units mL^-1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h^-1. Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4℃ was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH₄⁺-N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH₄⁺-N, making seed culture easier and suitable for public use.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.757-763
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• 2009

ISSR PCR technique was applied to investigate genetic relationship among 5 Mountain cultivated ginseng populations (Jinan, Hongcheon, Punggi, Andong and Yeongju) and cDNA libraries of wild ginseng roots were constructed and analyzed functional genes related to morphogenesis via EST. Twenty four ISSR markers tested produced 127 polymorphic loci from 5 regional Mountain cultivated ginseng. Among the regional samples, Yeongju was made 18 polymorphic loci that were the highest level of variations among the cultivated regions. The range of similarity coefficient was 0.46~0.58 and the regional samples of Punggi and Hongcheon, Jinan and Andong were classified to similar groups respectively, whereas Yeongju was shown to be separate group with high level of genetic variation in UPGMA cluster analysis. As a result, there was no relationship according to geographical distance and genetic similarity. Eleven cDNA clones were consisted of 9 known genes and 2 unknown genes analyzed by BLAST program of NCBI. To recognize expression pattern of Homeodomain transcription factor related genes, Northern Blot analysis was performed for wild ginseng's leaf and root. As a result, the gene was only expressed by Mountain wild ginseng root.

• Journal of Ginseng Research
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• v.40 no.2
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• pp.176-184
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• 2016

Background: Wild ginseng, Panax ginseng Meyer, is an endangered species of medicinal plants. In the present study, we analyzed variations within the ribosomal DNA (rDNA) cluster to gain insight into the genetic diversity of the Oriental ginseng, P. ginseng, at artificial plant cultivation. Methods: The roots of wild P. ginseng plants were sampled from a nonprotected natural population of the Russian Far East. The slides were prepared from leaf tissues using the squash technique for cytogenetic analysis. The 18S rDNA sequences were cloned and sequenced. The distribution of nucleotide diversity, recombination events, and interspecific phylogenies for the total 18S rDNA sequence data set was also examined. Results: In mesophyll cells, mononucleolar nuclei were estimated to be dominant (75.7%), while the remaining nuclei contained two to four nucleoli. Among the analyzed 18S rDNA clones, 20% were identical to the 18S rDNA sequence of P. ginseng from Japan, and other clones differed in one to six substitutions. The nucleotide polymorphism was more expressed at the positions 440-640 bp, and distributed in variable regions, expansion segments, and conservative elements of core structure. The phylogenetic analysis confirmed conspecificity of ginseng plants cultivated in different regions, with two fixed mutations between P. ginseng and other species. Conclusion: This study identified the evidences of the intragenomic nucleotide polymorphism in the 18S rDNA sequences of P. ginseng. These data suggest that, in cultivated plants, the observed genome instability may influence the synthesis of biologically active compounds, which are widely used in traditional medicine.

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.73-78
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• 2021

We report a technique for obtaining seeds of 'Cheongnarae' (a variety of Hosta minor cultivated at the Korea National Arboretum in 2019) by artificial crossing of H. minor, collected from Jeju Island, as the female parent and H. minor 'Krossa Regal', cultivated outside Korea, as the male parent. Among the individuals obtained after sowing, those that showed different morphological characteristics, such as leaf shape and color, were selected. Among these individuals, those with thick leaves and light-colored flowers were further selected. Vegetative propagation and cultivation steps were repeated for evaluation of the characteristics. 'Cheongnarae' has relatively thicker leaves and a darker color (RHS 137A) than the control variety 'Black Hills' and has wings at the point where the petiole meets the leaf blade. The shape of the leaf blade edge is clearly sinuous, and the flower color is close to white (White N155B). 'Cheongnarae' cultivated in this way can be used for pot plant or gardening.