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Genetic Analysis of 5 Mountain Cultivated Ginseng and Wild Ginseng in Korea  

Ahn, Ji-Young (Department of Environmental Science and Ecological Engineering, Dongguk University)
Kang, Sang-Gu (School of Biotechnology, Yeungnam University)
Kang, Ho-Duck (Department of Environmental Science and Ecological Engineering, Dongguk University)
Publication Information
Journal of Korean Society of Forest Science / v.98, no.6, 2009 , pp. 757-763 More about this Journal
Abstract
ISSR PCR technique was applied to investigate genetic relationship among 5 Mountain cultivated ginseng populations (Jinan, Hongcheon, Punggi, Andong and Yeongju) and cDNA libraries of wild ginseng roots were constructed and analyzed functional genes related to morphogenesis via EST. Twenty four ISSR markers tested produced 127 polymorphic loci from 5 regional Mountain cultivated ginseng. Among the regional samples, Yeongju was made 18 polymorphic loci that were the highest level of variations among the cultivated regions. The range of similarity coefficient was 0.46~0.58 and the regional samples of Punggi and Hongcheon, Jinan and Andong were classified to similar groups respectively, whereas Yeongju was shown to be separate group with high level of genetic variation in UPGMA cluster analysis. As a result, there was no relationship according to geographical distance and genetic similarity. Eleven cDNA clones were consisted of 9 known genes and 2 unknown genes analyzed by BLAST program of NCBI. To recognize expression pattern of Homeodomain transcription factor related genes, Northern Blot analysis was performed for wild ginseng's leaf and root. As a result, the gene was only expressed by Mountain wild ginseng root.
Keywords
EST; genetic analysis; ISSR PCR; wild ginseng; mountain cultivated ginseng;
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