• Fisheries and Aquatic Sciences
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• 제10권3호
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• pp.113-118
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• 2007

Bromophenols are a group of compounds found only in marine organisms. They accumulate and give a sea-like aroma to marine animals. Cultivated fishes generally contain low concentrations of bromophenols compared to wild fishes. Feeding cultivated fishes with bromophenol-containing seaweed could increase their bromophenol content and thus improve their flavor quality. We evaluated the effect of an experimental feed on the bromophenol content of green grouper, Epinephelus coioides, during an 8-week feeding period. Green grouper individuals were divided into two groups and fed with conventional feed or experimental feed containing dried seaweed. Fish were collected biweekly for 8 weeks for proximate analyses and bromophenol content evaluations. Bromophenols were extracted, identified, and quantified by gas chromatography-mass spectrometry. Both moisture and lipid contents were generally higher in the controls; however, total weight and protein content were higher in the experimental group. Only 2,4-dibromophenol and 2,4,6-tribromophenol were detected in the samples. Throughout the 8 weeks, 2,4,6-tribromophenol concentrations were higher in the experimental group (9.20-32.3 ng/g dry wt) than in the control group (7.33-18.79 ng/g dry wt), but no significant difference in 2,4-dibromophenol concentration was detected between the two groups. The total bromophenol content reached a maximum at week 4 for the experimental feed and week 6 for the control. In short, experimental feed that incorporated bromophenol-containing seaweed increased the total bromophenol content in the green grouper.

• Journal of Plant Biotechnology
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• 제37권3호
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• pp.269-274
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• 2010

As the capture fishing industry has declined, the aquaculture industry has become an important source of seafood. With this tendency all fish farming will be performed by large-scale farms where the fish are cultivated in much high density and as a result the incidence of infectious diseases increases. Therefore, vaccination has become an increasingly important part of aquaculture as a cost effective method of controlling various diseases. The early fish vaccines were the formalin inactivated bacteria or virus cultures, which were administered by either immersion or injection. Recombinant DNA biotechnology allowed us to develop orally administrated DNA and recombinant vaccines. In terms of the manufacturing process and cost, Lemna and Spirodela is the most efficient and reliable plant expression system for the production of edible vaccine.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.569-581
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• 2023

The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.223-227
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• 2021

재배종 고구마 [Ipomoea batatas L. (Lam)]의 정확한 기원종은 아직 밝혀지지 않았으며 다양한 가설들이 보고되었다. 재배종과 동일한 batatas 그룹에 속한 야생종을 대상으로 RFLP 패턴, microsatellite 마커, SNP 마커, FISH 기법, 반수체에 대한 게놈분석 등의 비교 연구에 의하면 I. trifida, I. leucantha, I. littoralis, I. tabascana, I. tenuissima, I. tiliacea, I. triloba 등이 가능성이 있는 것으로 제안되었다. 그러나, 최근의 진보된 유전체분석기술과 자연상태에서 수평전이현상에 의하여 재배종 고구마 및 다양한 야생종들의 염색체 내 삽입된 T-DNA의 유전자 구조, 삽입위치, 유전자 재배열 등의 특성을 조사한 연구 결과들을 종합하여 보면 기존에 제안된 이들보다 오래된 조상종이 존재할 것이라는 새로운 가설이 제시되었다.

• 동아시아식생활학회지
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• 제15권5
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• pp.493-503
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• 2005

This survey was performed to investigate the distribution of specialities which cultivated importantly in Jeollabukdo area in order to understand food culture of this area. Foods in speciality were divided into 4 groups, agricultural, aquatic, animal and the others products. Total number of 93 species of specialities, including 40 species of agricultural products, 29 species of aquatic products, 8 species of animal products and 16 species of the miscellaneous products, were identified from Jeollabukdo area. Especially, there were various fruits and vegetables among agricultural products and fishes among aquatic products in this region. Rice, pepper, pear, Mandarin fish, beef, pine mushroom, lentinus edodes and honey were determined as specialities in Jeollabukdo area. Various aquatic products were specialities in Gochang, Gunsan, Buan and Gimje region. Fruits, mushrooms, wild edible greens and animal products were specialities in Muju, Jangsu, Jeongeup and Jinan. Sweet fish, cat fish and freshwater crab were caught off in Sumjin river region only such as Namwon, Sunchang and Imsil, Recently, pumpkin(Cucurbita maxima) and paprika were determined as specialities in Jeollabukdo. In case of aquatic products, mushrooms and wild edible greens, their wild type products decreased, while cultivated type increased Commercialization by brand naming of agricultural and animal products were rapidly increasing. The kinds of specialities in Jeollabukdo area were changing by the influence of geographical, religious and social factors.

• 한국수산과학회지
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• 제29권3호
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• pp.279-286
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• 1996

틸라피아를 해수에 순치하여 근육의 물성 및 식감을 개선하기 위하여 해수에 순치한 틸라피아를 $0^{\circ}C,\;10^{\circ}C$ 및 $20^{\circ}C$에 저장하면서 사후경직 중에 나타나는 근육의 물리적, 화학적 변화를 조사하였다. 사후경직은 $10^{\circ}C$와 $20^{\circ}C$ 에서보다 $0^{\circ}C$에서 빠르게 진행되었으며, 담수 사육한 것보다 해수 순치한 것이 경직개시 시간이 빨랐다. 사후경직 중 파괴강도와 사후 경직도와의 상관 관계는 없었으며, 파괴강도는 사후 12시간만에 최대로 되었고, 그 이후로는 육의 연화가 일어남으로 인하여 급격하게 저하된 반면에, 사후 경직도는 계속해서 증가하여 18시간 후에 최대치를 나타내었다. ATP 분해속도는 담수 사육어보다 해수 순치어에서 빨랐으며, 저장온도에 따른 분해속도는 $10^{\circ}C$와 $20^{\circ}C$에 비하여 $0^{\circ}C$에 저장한 시료에서 빠르게 나타났다. IMP 및 젖산은 저장온도가 낮을수록 축적속도가 빨랐고, 담수 사육어보다 해수 순치어에서 빠르게 나타났다. 이상의 결과로부터 담수어인 틸라피아를 해수에 순치시킴으로써 사후초기에 경직을 유도하고 근육의 물성을 변화시켜 식감을 증진시킬 수 있을 것으로 생각된다.

• 한국가금학회지
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• 제22권3호
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• pp.155-160
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• 1995

To evaluate the nutritive values of outdoor mass cultivated Spirulina platensis both chemical analysis and bioassay were carried out using adult cockerels. Blue-green algae, Spirulina platensis contained about 71g /l00g DM of crude protein with balanced amino acid profiles although methionine is liable to he limiting to animals. Compared to fish meal, calcium content and calcium : phosphorus ratio of the Spirulina were not suitable in terms of animal requirements. Reasonable amount of y-linolenic acid(C18: 3 $\omega$6) in Spirulina platensis draws a clinical attention due to its historically recognized pharmacotheraputic functions. Metabolizable energy contents of Spirulina were 3.67 and 3.11 mcal /kg DM for TMEn and AMFn, respectively, which therefore, can he a reliable energy source for poultry. True amino acid availabilities of essential amino acids of Spirulina platensis were higher than 90% for poultry, which is better than comparative ingredient like fish meal. Overall data from both chemical analysis and bioassay demonstrated that the Spirulina platensis could he a favorable protein feedstuffs for poultry.

• 한국식생활문화학회지
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• 제4권2호
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• pp.191-197
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• 1989

Before Jin (秦) period, Oriental (Eastern) culture was established in Korea different from China. Bulgogi (babecued beef, 貊炙) and legume fermented soy were transmitted into China. Afterward, alcohol drink, rice cake and cookie, shic-hae (lactic acid fermented fish products), Kimchi (fermented vegetable) were introduced and modified for Korea. Buddhism was transmitted to Korea through China, but selective animal was used as food. Later period of Koryo Dynasty, meat-eating become common due to mongorian influence and distilled spirits was introduced by mongorian. During Chosun Dynasty, table setting of spoon and chopstick was established, due to Confucian influence, dog eating, raw fish and raw meat eating became popular and nutrition for elderly was developed, whereas tea culture declined. In recent period (under the Japanese rule) Chinese introduced chinese noodle, chinese cuisine, chinese pancake and sun-dried salt. many chinese cultivated chinese vegetables.

• 한국식품과학회지
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• 제28권6호
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• pp.1089-1094
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• 1996

검은툭눈붕어를 이용하여 tyrosinase 저해제가 어체의 melanogenesis에 미치는 영향을 평가하였다. 저해제로는 glutathione, cysteine, benzoic acid를 각각 5, 5, 1 mM씩울 혼합하여 첨가한 식품첨가물 처리구, 표고버섯을 주기질로 하여 Aspergillus oryzae를 발효시켜 얻은 microbial inhibitor 처리구, 설록차, 무, 팽이버섯, red chicory 등 4종의 식물체 추출액 혼합물 처리구로 나누어 효능을 평가하였다. 검은툭눈붕어를 0.9% NaCl 용액에서 일주일간 양식하면서 stress를 가하여 비늘의 melanin 농도를 높이고 다시 민물에 담고 각 처리구의 저해제를 첨가한 용액에서 6일간 양식한 후 가슴 부위, 측선 부위 및 등지느러미의 색을 색도계로 측정하고 현미경으로 melanin을 관찰하였을 때 식품 첨가물 처리구와 microbial inhibitor 처리구의 색은 대조구보다 명도와 yellowness가 높아진 것으로 나타나 melanogenesis 억제 효과가 뚜렷하였다. 한편, 농산물 처리구의 경우에도 미약하지만 효과가 인정되었다. 저해제를 처리한 검은툭눈붕어와 대조구 붕어의 측선 부위 비늘과 등지느러미를 설취하여 현미경으로 관찰한 결과 저해제 처리구에서는 단위 면적당 melanophore의 수가 감소하고 하나의 melanophore의 크기도 작아진 것으로 나타났다.

• 미생물학회지
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• 제40권2호
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• pp.133-138
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• 2004

본 연구는 2002년 6월부터 2003년 11월까지 복수증을 나타내는 양식넙치의 세균상과 virus를 동정한 것이다. 생화학적 성상을 기초로 한 표현형으로, 병어체의 복수, 간, 신장, 내장으로부터 Vibrio, Edwardsiella, 및 stonella 등 3개 속이 동정되었다. Vibrio속은 5종으로 우점이었고 V. harveyi (32균주), V. parahaemolyticus (23균주), V. alginolyticus (10균주), V. carcariae (2균주), V. metschnikovii (5균주)였다. 그밖에 Photobacterium damselae (6균주)와 장내세균인 Edwardsiella tarda (15균주)가 동정되었다. 감염어의 내부 장기로부터 해산어 병원성 바이러스로 알려진 marine birnavirus (MABV)가 분리되었다.