• Proceedings of the Plant Resources Society of Korea Conference
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• 1993.08a
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• pp.14-20
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• 1993

We in anticancer drug development from natural resources have conceived and used a wide variety of experimental screening systems to support our efforts during the past 20 tears. Screens have been devided to address targets at the molecular, biochemical and cellular levels, both in vivo and in vitro. Screens have been essential for the experimental evaluation of the products from natural sources. In this congress, antitumor screening methods for deveol[ment of new drugs from natural sources and evaluation of their crude drugs are discussed.

• YAKHAK HOEJI
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• v.19 no.2
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• pp.65-78
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• 1975

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.86-86
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• 1990

• Korean Journal of Oriental Medicine
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• v.2 no.1
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• pp.506-513
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• 1996

The odor theory(氣味論) has been defined as the Nature(氣) and the Taste(味) of traditional herbal medicine to find the logic in treatment of various diseases by them. There is a strong possibility these Natures of the drug can be categorized according to yin(陰) and yang(陽). To understand the Hot and the Cold Natures of traditional herbal drugs in scientific approaches, changes in skin, rectal temperature of rats have been measured at 0, 30, 60, 90 min after a 5, 10, 20g/Kg oral administration each of Coptidis Rizoma(Ranunculaceae, Coptis chinesis, $F_{RANCH}$), Aconiti Lateralis Preparata Radix(Ranunculaceae, Aconitum carmichaeli $D_{EBX}$). From this study, we obtained as follows. 1. We can categorize the four Natures of drug according to yin(陰) - Cool and Cold Natures and yang(陽) - Warm and Hot Natures. 2. The relation between the four Natures of drug and the changes of body temperature can be studied as the considertion of the dosage and the preparation of crude medicines. 3. Herbs containing toxicant should be studied carefully without the side effects.

• YAKHAK HOEJI
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• v.42 no.2
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• pp.214-219
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• 1998

Tissue distributions and association of cyclooxygenase-2 (COX-2) with inflammatory have led us to search for COX-2 selective inhibitors from natural products. Conceptually, COX- 2 selective inhibitors should be expected to retain anti-inflammatory efficacy by inhibition of PGs production while reducing or eliminating the gastric, renal and hemostatic side effects commonly associated with NSAIDs use. Thus, a logical approach to the treatment of inflammatory diseases should involve the inhibitors of COX-2. To develop new COX-2 inhibitors from natural products, two hundred crude drugs were screened by inhibiting PGD2 generation in bone marrow derived mast cells (BMMC). Among them, 6 methanol extracts of crude drugs such as, Bletillae rhizoma, Aconiti kgreani rhizoma, Belamcandae rhizoma, Nelumbinis semen, Gleniae radix, Aurantii immatri pericarpium inhibited more than 85% of BMMC COX-2 activity at a concentration 2.5${\mu}$g/ml.

• Korean Journal of Pharmacognosy
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• v.16 no.4
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• pp.214-220
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• 1985

The preservatives in 32 commercial liquid preparations of crude drugs have been analyzed by using high performance liquid chromatography. Benzoic acid (BA) and p-hydroxybenzoates were separated with mobile phase of $MeOH-H_2O$ (70:30) (pH 7.0) at a flow rate of 0.9ml/min. BA, sorbic acid and dehydroacetic acid were separated with mobile phase of $MeOH-H_2O$ (40:60) (pH 4.5) at a same flow rate. In addition, the pH values of the preparations were investigated. It was found that BA was used in most of the preparations.

• Korean Journal of Pharmacognosy
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• v.13 no.2
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• pp.79-86
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• 1982

In order to investigate pharmacological actions of combined preparations of crude drugs, 'Jakyakgamcho-Tang' were studied. The 'Jakyakgamcho-Tang' using abdominal pain and convulsion of pediatric diseases are composed of Glycyrrhizae Radix and Paeoniae Radix alba. This study was undertaken to investigate the effect of 'Jakyakgamcho-Tang'(S-I) and parched 'Jakyakgamcho-Tang'(S-II) on anticonvulsive, analgesic, antipyretic and sedative actions. The results of this study were summarized as follows; Both 'Jakyakgamcho-Tang' and parched 'Jakyakgamcho-Tang' showed anticonvulsive effects against convulsions induced by strychnine, picrotoxine and caffeine. 'Jakyakgamcho-Tang' and parched 'Jakyakgamcho-Tang' also showed analgesic and antipyretic effects. Parched 'Jakyakgamcho-Tang' only prolonged the duration of hypnosis induced by sodium pentobarbital.

• Korean Journal of Pharmacognosy
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• v.3 no.4
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• pp.205-209
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• 1972

Anti-inflammatory activity of crude drugs was evaluated by the albumin stabilizing activity test, according to the screening method of Mizushima et al., upon the randomly selected samples of 63 genus, 106 families, 123 species of plant. Almost every plant belonging to the families Araliaceae, Umbelliferae and Liliaceae showed strong stabilizing activity on the heat denaturation of bovine serum albumin, suggesting the presence of anti-inflammatory components in the plants.

• Korean Journal of Pharmacognosy
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• v.31 no.1
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• pp.16-22
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• 2000

These studies were designed to determine the potential cytotoxic activity of MeOH extracts of 65 crude drugs against leukemia L1210 and $P388D_1$, cell line in vitro, of which 25 samples were selected. The n-BuOH extracts of 25 samples were measured using the same method and nine were selected. These samples were measured for the potential antitumor activity against $P388D_1$, for life span in vivo, and against sarcoma 180 for tumor weight. On the basis of results, Notoginseng Radix, Anemarrhenae Rhizoma, Albizziae Cortex, Portulacae Herba, Bupleuri Radix were evaluated the effective plant on the antitumor activity. In addition, the mixture of Notoginseng Radix and Albizziae Cortex was evaluated to enhance the antitumor activity in vivo.

• Korean Journal of Pharmacognosy
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• v.19 no.4
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• pp.270-277
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• 1988

Gamiinjinoryung-San is composed of eight crude drugs including Artemisiae capillaris Herba which is widely used for the treatment of acute jaundice, acute and chronic hepatitis at the Oriental hospital of Kyung-Hee Medical Center. This study was conducted to investigate the effects of water extract of Gamiinjinoryung-San on the liver function. The results obtained were as follows; the extract markedly reduced LDH and ALP activities, but slightly decreased GOT and GPT activities elevated with D-galactosamine in rat serum. The liver protective activities were shown in $CCl_4-intoxicated\;rats$. The extract prevented the prolongation of sleeping time in $CCl_4-intoxicated\;rats$. The bile flow and the biliary bile acid secretion were significantly increased in normal rats.