• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.2
• /
• pp.169-178
• /
• 2010

The present study was conducted with four ruminally fistulated cattle to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility and TDN values of various silages (whole crop rye, RS; whole crop barley, BS; Italian ryegrass, IRGS and sudangrass, SGS) which were produced in Korea. The pH of rumen fluid were not greatly affected by silage at most times after feeding but SGS decreased (p<0.049) the ruminal ammonia-N concentration as 7.28 mg/100 ml at 3h after feeding compared to other silages. An acetate proportion from SGS feeding was higher at 1h (p<0.018), 3h (p<0.004) and 6h (p<0.019) after feeding than those from other silages. The propionate ($C_2$) proportion, however, was greater (p<0.001~p<0.042) for the RS and BS than the corresponding values of other silages up to 6h after feeding while the lowest $C_2$ proportion was observed from SGS. The butyrate proportion was lowest (p<0.007~p<0.027) in the cattle fed BS at 1~6h after feeding among silages. An ED of dry matter (DM) in the rumen was highest (P<0.048) for RS as 59.64%, and was reduced in the order of BS (56.12%), IRGS (55.64%) and SGS (54.02%). Similar tendency was observed in the EDs of crude protein (p<0.014) and organic matter (OM, p<0.039). The whole tract digestibility of DM (p<0.032), neutral detergent fiber (NDF, p<0.034) and OM (p<0.041) of SGS was greatly reduced. The TDN value based on whole tract digestibility of silages was highest for RS as 61.1%, and TDN values of BS, IRGS and SGS were 57.1%, 57.9% and 50.7%, respectively. Based on the results obtained from the present study, components in various whole crop silages produced in Korea might affect the ruminal VFA proportion, and especially, NDF content could greatly influence on the TDN value of silage calculated based on the NDF content.

• Korean Journal of Organic Agriculture
• /
• v.23 no.2
• /
• pp.359-372
• /
• 2015

This study was to assess the effects of sowing mixed legume forage and applying fermented cattle manure on the productivity and feed value of whole crop wheat (WCW) as a representative of winter crops and also to estimate feeding capacity of Korean native cattle (Hanwoo) per unit area by defining optimal application levels of cattle manure for more fertile soil and manure recycling for the purpose of reduced environmental pollution and efficient production of organic forage contributable to production of organic animal products. Sowing mixed legume forage significantly (p<0.05) increased yields of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) for WCW compared to WCW alone sowing, but there were no differences between mixed sowing treatments. The yields of DM, CP and TDN were significantly (p<0.05) higher in 100~150 kg N/ha. The CP and acid detergent fiber (ADF) contents were highest in mixed sowing with hairy vetch, followed by sowing mixed forage pea and WCW alone (p<0.05). There were no differences in CP among cattle manure application levels. As nitrogen application level increased, ADF content increased (p<0.05), but TDN content decreased (p<0.05). Feeding capacity of organic Hanwoo (head/ha) weighing 450 kg of body weight with 400 g of daily gain significantly (p<0.05) increased due to mixed sowing with legume forage, and with increasing application levels of fermented cattle manure, feeding capacity significantly (p<0.05) increased. The results indicated that mixed-sowing of WCW and legume forage not only reduced nitrogen application level of fermented cattle manure by over 50 kg per ha but also increased their feed value and productivity, and feeding capacity. This would be contributable to diversity of forage resources as well as production of organic animal product by creating low cost and high quality of forage.

• The Korean Journal of Mycology
• /
• v.30 no.2
• /
• pp.136-141
• /
• 2002

A hot water extract was prepared from the artificially grown Gastrodia elata to investigate its chemical composition and various in vitro biological activities as an effort to develop G. elata as health/functional food materials. The contents of crude protein, ash, fat, fiber, moisture and total sugar were 5.4, 2.6, 3.6, 3.3, 8.1 and 77% (w/w), respectively. The extract of G. elata had greater amount of potassium (1,150 mg/100 g) than phosphorus (300 mg/100 g). Dose-dependence against human carcinoma (Hep3B, MCF-7, A549 and AGS) were observed from 0.2 mg/ml to 1.0 mg/ml. Especially, the treatment of 1.0 mg/ml extracts showed the highest cytotoxicity with 83% against gastric carcinoma (AGS). The extracts showed weak antimicrobial activities against Bacillus subtilis and Pseudomonas aeruginosa, but practically no antimicrobial activity against the other microorganisms tested. The effect of ${\alpha}$-glucosidase inhibition was 64% at the concentration of 1.0 mg/ml. The inhibitory effect of angiotensin converting enzyme (ACE) of the extract in the range of $0.2{\sim}1.0mg/ml$ showed $63{\sim}89%$, and the highest ACE inhibition was 89% at the concentration of 1.0 mg/ml of extracts. The highest activity of glutathion S-transferase (GST) was 221 % at the concentration of 1.0 mg/ml of the G. elata extracts. These results suggest that G. elata may be used as health/functional food materials.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.1
• /
• pp.92-98
• /
• 2010

Physicochemical properties, free sugar and volatile flavor compounds of citrons harvested in different cultivation areas, such as Geoje, Goseong, Goheung, and Namhae, were analyzed and compared. Total weight of citron, ranging from 107.97 g to 154.86 g, was significantly different according to producing area. Weight ratio of flesh to whole citron was higher than one of peel in citron produced from Geoje, Goheung-improved, or Namhae-native. Citron size was the highest in Namhae-native citron, but the color of peel was significantly the higher in Goheung-native and its improved citron. In the peel of citron, hardness of citron produced from Geoje was the strongest (2337.13 $cm/kg^2$) whereas citron produced from Goseong (1592.38 $cm/kg^2$) showed the softest hardness. Moisture content was 85.35~87.81 g/100 g and ash was contained below 0.8 g/100 g in flesh of all samples. Although crude fiber content in Geoje ctiron was significantly the lowest in the peel, peel was 1.88~2.60 times higher than flesh. The order of sugar content seemed to be fructose> glucose> sucrose in all citrons, and content of fructose or glucose was higher in peel than flesh. Content of total sugar was 8.44~12.19 g/100 g in citron, and contents of improved citrons from Goheung and Namhae were significantly higher than those of native citrons. In addition, major volatile compounds were dl-limonene (59.52~74.30%) and $\gamma$-terpinene (5.60~7.88%) among 15 kinds of volatile compounds identified from citron peel. These results suggested that physicochemical properties and volatile flavor compounds of citrons showed some differences with regard to producing areas.

• Korean Journal of Food Science and Technology
• /
• v.38 no.4
• /
• pp.475-482
• /
• 2006

The quality characteristics and functional properties of fermented soybean by lactic acid bacteria (FSB) were investigated and compared with those of soybean (control) and commercial cheonggukjang Powder (CGP). The crude protein, lipid, and fiber contents of FSB were similar to those in CGP. The vitamin $B_2$ content in FSB (1.4 mg%) was similar to the control (1.3 mg%) whereas it was remarkably low in the CGP (0.2 mg%). The bright yellow color of FSB determined by Hunter's colormeter was quite different from the dark reddish brown color of the CGP. The pH of FSB was the lowest and thee amino-nitrogen was 517.2 mg%, which was higher than that in CGP (468.1 mg%). Glutamic acid, aspartic acid and leucine were the most abundant amino acids. In particular, the increase in the glutamic acid level was noticeable in FSB. The fatty acid compositions of FSB and CGP were similar to the control. However, the sucrose and fructose levels were lower after fermentation but the glucose level was higher. The results of isoflavone analysis by HPLC showed that the levels of daidzein and genistein in FSB were as much as 48.33 and 52.82 mg%, respectively, which is higher than that found in CGP. The DPPH free radical scavenging effects of FSB and CGP were 1.8 times higher an those of the control. The fibrinolytic activity determined by the diameter of the lysed area on the fibrin plate was the most effective in FSB. In conclusion, it is believed that FSB has a similar or higher quality than CGP. Therefore, FSB is expected to be good functional food material.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.35 no.1
• /
• pp.50-56
• /
• 2015

Italian ryegrass is generally cultivated by a winter forage in a paddy field, the harvest season overlaps with rice transplantion in some cases. In addition, farmers can miss the optimal seedtime for Italian ryegrass because of the rainy season. Therefore, this research was conducted between October 2012 and June 2013 in Kwangjin, Korea to examine the effect of spring-seeding on the yields and quality of Italian ryegrass for the determination of an alternative if the optimal seedtime is missed. This trial consists of four different seeding dates (15 February, 25 February, 5 March, and 15 March) and three varieties (Kowinmaster, Kowinearly, and Green farm). The Italian ryegrass varieties "Kowinmaster," "Kowinearly," and "Green farm" were sown on February 15. The heading date of each variety showed from May 12 to May 9 and interval time of five days but the Treatment of March 15 planting date showed three days interval. The Kowinmaster variety, planted on February 15, was 93.0 cm taller on March 15 with a height of 105.7 cm. The Kowinearly and Green farm varieties planted on February 15 were of a similar height on February 25 and received a poor lodging score when planted quickly. The dry matter (DM) yield of the Kowinearly planted on February 15 was high but that of Green farm was low at 6,609 kg/ha and a difference was not shown between the varieties. The DM yield was different for those varieties planted on February 15 and March 15 (P<0.05). The crude protein content (CPC) of those varieties planted on February 15 showed an average of 8.8%, while those that were planted on March 15 showed an average that was about 2.3% higher at 11.1%; furthermore, the latter also showed lower acid detergent fiber (ADF) and total digestible nutrient (TDN) contents, by 2.1% and 1.5%, respectively. The relative feed value was about 5.9% higher, depending on the seeding date, as well as being slower and showing less difference between the varieties. According to this study, Italian ryegrass planted in a paddy field of the southern region of Korea on February 25 will produce a high-quantity yield with nutritive value.

• Applied Biological Chemistry
• /
• v.13 no.1
• /
• pp.1-27
• /
• 1970

The process of the forced aging of flue-cured tobacco leaves were studied extensively from various scientific points of view. The Flue-cured tobacco leaves were inoculated and fermented with nicotine resistant Hansenula yeast, or the leaves were subjected under simple forced aging. The above two processes of forced aging were studied from the summarized points of microbiology, physics, chemistry, and biochemistry, and the resulted products ware compared in their physical, chemical and biochemical quality determining factors with that of raw material tobacco leaves (dried-tobacco leaves) and 2 years aged high quality tobacco leaves. The summary results were as follows. 1) The Korean flue-cured tobacco leaves, were forcedly aged under the basic optimum aging condition, temperature $40^{\circ}C$, moisture contents 18%, relative humidity 74%. It was found that this aging condition was the best in bringing the quality of forcedly aged tobacco leaves to the utmost state. 2) Under this optimum temperature and moisture condition of forced aging in about 20 days the forcedly aged tobacco leaves both with yeast inoculation and without yeast inoculation showed the equivalent tobacco qualities comparable with that of more than 2 years aged tobacco leaves. 3) The forcedly aged tobacco leaves both with and without yeast inoculation under $40^{\circ}C$ temperature and $74^{\circ}C$ relative humidity achieved the necessary quality determining physical and chemical changes in about 20 days. 4) The microbial changes during the forced aging were as follows. The population of yeasts and bacteria increased until to 15 days of aging, then decreased thereafter. Whereas the molds grew continously until the end of fermentation. 5) The tobacco quality determing physico-chemico-properties of yeast inoculated aged and simple forcedly aged tobacco leaves, progressed as the follows in time. As the forced aging progresses, swelling and combustibility properties were improved. The pH, total reducing materials, total sugars, alkaloids contents decreased. The contents of organic and ether extractable materials increased. The total nitrogen, protein, crude fiber, ash contents showed no changes. The color properties, excitation purity, luminance, main wave length, showed equivalent changes comparable with that of 2 years aged tobacco leaves. 6) The changes in chemical components in yeast treated and simple forcedly aged tobacco leaves during $15{\sim}20{\;}days$ of forced aging were as follows. The following chemical components decreased as the aging. Sugars-sucrose. rhamnose, glucose. Pigments-chlorophyll, carotenes, xanthophyll and violax anthine. Polyphenols-rutin, chlorogenic and, coffeic acid. Organic acids-iso-butylic, crotonic, caprylic, galacturonic, tartaric, succinic, citric acid. Alkaloids-nicotine, nornicotine. The following components increased as the forced aging progressed. Sugars-frutose, maltose, raffinose. Amino acids-proline, cystine. Organic acids-formic, acetic, propionic, n-butyric, iso-valeric, n-valeric, malic, oxalic, malonic, ${\alpha}-ketoglutaric$, fumaric, glutaric acid. 7) During the forced aging of tobacco Leaves the oxygen-uptake decreased gradually. The enzyme activities of polyphenol oxidase, ${\beta}-amylase$ ${\alpha}-amylase$ decreased gradually. The activities of the enzymes, catalase, and invertase increased once then decreased at the later stage.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.1 no.1
• /
• pp.18-28
• /
• 1978

To study about the effect of harvested stage and physical treatment such as wilting or chopping of plant material on the quality of silage, Italian ryegrass(Lolium multiflorum) harvested at pre-headed or heading stage and Seombadi(Dystaenia takesimana Nakai) harvested at pre-flowering stage, were used for the purpose. The materials were treated in four different ways which were a) non-treated, b) one day-wilted, c) chopped with 1 to 2cm length and d) wilted and chopped, and those were ensiled in plastic containers and stored at room temperature for three months. After three months of storage, it was investigated and obtained the following results. 1) Having 6.31 of NFE/CP ratio and low content of crude fiber, Seombadi contained more suitable constituents for silage than Italian ryegrass. 2) Under the non-treated or chopped condition, the loss of NFE was higher, and lower with wilted material. 3) Wilting or chopping improved DM digestibility. 4) Compared with Italian ryegrass harvested at pre-headed stage, the storage amount per unit volume of heading stage-Italian ryegrass and Seombadi were higher 8% and 69% respectively, and wilting and/or chopping increased the storage amount 41 to 134%. 5) The majority of weight loss during storage was observed at 1st week after ensiling, it continued slightly until 4th week. The highest loss in 8th week were 3.76% of nontreated material. 6) One day wilting increased DM content of silage 23 to 131%. Wilting and chopping increased pH and lactic acid improved the quality considerably. 7) The correlation between $NH_3$ and butyric acid, and between lactic acid and DM were r=0.782**, r=0.634** respectively. The regression equation were y=12.853X+4.908 (X=butyric acid), y=0.016X+1.309(X=DM content), respectively. 8. The above results indicate that it is necessary to wilt or chop material to make good quality silage from Italian ryegrass, and such treatment can improve the quality of silage with Seombadi also.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.40 no.1
• /
• pp.57-65
• /
• 2020

This study was conducted to investigate the effect of sowing and harvesting dates on agronomic characteristics and feed values of rye and triticale at Sanchoeng, South Korea. The experimental design consisted of the different sowing and harvesting dates as follows; rye (Secale cereale L., cv. Gogu) of sowing (October 15, 25, and November 5) in 2015 and harvesting (April 20, May 1 and May 11) in 2016, and triticale (X Triticosecale, cv. Joseong) of sowing (October 15, 25, and November 5) in 2015 and harvesting (May 18, 28, and June 7) in 2016. In rye, fresh and dry matter (DM) yields increased (p<0.05) with the delayed-harvesting date. Crude protein (CP) content and relative feed value (RFV) decreased (p<0.05) with the delayed-harvesting date, but neutral detergent fiber (NDF) content increased (p<0.05). In triticale, fresh and dry matter (DM) yields increased (p<0.05) with the delayed-harvesting date. The CP content decreased (p<0.05) with the delayed-harvesting date, but NDF content and RFV increased (p<0.05). This study concluded that rye sown in the middle of October then harvested in early May, and the triticale sown at the end of October then harvested at the end May are recommended to increase dry matter yield and feed value.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.25 no.3
• /
• pp.151-158
• /
• 2005

This experiment was conducted to evaluate forage production and quality, and soil improvement of three legumes at Cheonan Yonam College. The three legumes used in the experiment were crimson clover (Trifolium incarnatum L.) red clover(Trifolium pratense L.) and chinese milk vetch(Astragalus sinicus L.). Flowering stage was observed on the 20th of April for chinese milk vetch and on the 30th of April for crimson clover, but the red clover did not produce flower until harvest time. The dry matter(DM) content of crimson clover was the highest among the three legumes. In terms of DM yield, crimson clover has the highest yield, the highest yield in crude protein(CP) and total digestible nutrients(TDN) was red clover. In terms of forage quality, the highest CP content was red clover, while the chinese milk vetch was the lowest for both neutral detergent fiber(NDF) and acid detergent fiberr(ADF). Red clove. and chinese milk vetch were highest fur TDN content, and the chinese milk vetch has the highest for relative feed value(RFV). Assessment of the chemical properties of soil after harvest showed that the nitrogen content of soil increased in all legumes. The organic matter(OM) content of soil in both crimson and red clover increased, while the chinese milk vetch decreased. The available $P_2O_5$ decreased in all three legumes, but the biggest decrease was in the chinese milk vetch. The exchangeable potassium in the soil for both crimson and red clover increased, but in chinese milk vetch it decreased. Based on the results of this study, the chinese milk vetch was superior in terms of forage quality, but crimson and red clover are excellent in forage yield and in maintaining soil quality in upland.