• Research in Plant Disease
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• v.29 no.4
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• pp.363-376
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• 2023

Alternaria black spots or blights in cruciferous crops have been devastating diseases worldwide and led to economic losses in broccoli, Chinese cabbage, kale, radish, rapeseed, etc. These diseases are caused by different Alternaria spp., including A. brassicae, A. brassicicola and A. raphani transmitted from infected seeds or insect vectors. Efforts to excavate disease resistance traits of cruciferous crops against Alternaria black spots or blights have been demonstrated. Genetic resource of disease resistance was investigated in the wild relatives of cruciferous crops, and different cultivars were screened under different inoculation conditions. Development of the disease-resistant lines against Alternaria black spots or blights was also tried via genetic transformation of the cruciferous crops using diverse plant defence-associated genes. Plant immunity activated by pre-treatment with chemicals, i. e. β-amino-n-butyric acid and melatonin, was suggested for reducing Alternaria black spots or blights in cruciferous crops. The disease resistance traits have also been evaluated in model plant Arabidopsis originating from different habitats. Various plant immunity-related mutants showing different disease responses from wild-type Arabidopsis provided valuable information for managing Alternaria black spots or blights in cruciferous crops. In particular, redox regulation and antioxidant responses altered in the Alternaria-infected mutants were discussed in this review.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.655-666
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• 2022

We assessed the persistence of eight major cruciferous crops-leaf mustard, oilseed rape, cabbage, broccoli, cauliflower, Chinese cabbage, turnip, and radish-growing in the field. In the first part of our experiment, we tested the viability of seeds that had been buried at two different soil depths for up to 16 months. We then broadcast seeds over the soil surface and left them undisturbed to investigate the survivorship of the resultant plants over two years. Seed viability was significantly affected by plant taxa and burial depth, but not substantially affected by the duration of burial. Although seeds of leaf mustard had the greatest viability among all crops examined here, the viability rates were significantly lower at 2 cm depth than at 15 cm. Seeds of leaf mustard, oilseed rape, broccoli, turnip, and Chinese cabbage remained viable throughout the 16-month period. A study of plant demography revealed that only leaf mustard and oilseed rape succeeded in producing seeds and overwintering in the undisturbed field. However, neither of those species competed well with other plants long-term and their overall growth and survival rates declined during the evaluation period. In addition, insect herbivory severely decreased the growth of all of these crops. Our results suggest that populations of leaf mustard and oilseed rape do not tend to persist in the field for more than a few years without disturbance and external seed inputs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.78-78
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• 2020

Cruciferous plants such as broccoli and radish contain glucosinolate, which is a bioactive precursor that is most often used in Korean foods and is unique as a food ingredient. In addition, it contains various other phytochemicals and is promising as a health-oriented food material. In particular, Sulforaphane is a hydrolyzate of the glucosinolate, which has a more beneficial effect on the human body. Glucosinolate may be hydrolyzed by enzymes called myrosinase, which is voluntarily possessed by cruciferous plants. However, the ESP(Epithiospecifier protein) in broccoli sprouts could acts competitively with myrosinase, and convert to the less bioactive sulforaphane nitrile form. Therefore, we improved the yielding of sulforaphane in broccoli sprouts with a new method. We induce inactivation of the ESP protein by heat treatment. At this time, a myrosinase was introduced from the radish sprout because myrosinase in broccoli sprouts is also denatured by heat treatment. According to the results, we have confirmed by GC / MS that formation of sulforaphane increases more than 7 fold using set heating and mixing conditions.

• Research in Plant Disease
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• v.7 no.2
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• pp.93-101
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• 2001

Occurrence of Fusarium wilt was surveyed in fields of cruciferous vegetable crops in Korea from 1996 to 1998. The disease severely occurred up to 40% in fields of Chinese cabbage and radish but slightly in Fields of cabbage. A total of 123 isolates was obtained from roots of the diseased plants and identified as Fusarium oxysporum based on the morphological and cultural characteristics. Pathogenicity of nine isolates selected from the isolates was tested by artificial inoculation to the hosts. All the isolates had similar virulence on Chinese cabbage and cabbage, although there were some differences in virulence on cultivars tested among the isolates. The isolates from radish were more virulent to radish than those from Chinese cabbage and cabbage. All isolates from the crucifers were not virulent to eight species of vegetable crops except the crucifers. The results of pathogenicity tests showed that the pathotype of Chinese cabbage-infecting isolates was identical to that of cabbage-infecting isolates, but somewhat different from that of radish-infecting isolates.

• The Plant Pathology Journal
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• v.21 no.4
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• pp.369-376
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• 2005

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.69-74
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• 2003

Cruciferous crops grown in greenhouses and fields in Korea were surveyed from 1995 to 2000. Sclerotinia rot most severely occurred up to 30％ in cabbage. Incidence of the disease was as high as 20% at its maximum in Chinese cabbage and rape and 10％ in radish, but as low as less than 1 or 2％ in broccoli and kale. Symptoms of Sclerotinia rot commonly developed on loaves and stems of the crucifers, but rarely on rachises of broccoli. A total of 112 isolates of sclerotinia species was obtained from the diseased crucifers. Out of the isolates, 103 isolates were identified as S. sclerotiorum, and the rest as 5. minor based on their morphological and cultural characteristics. S. sclerotiorum was isolated from all the crucifers, while S. minor was isolated from Chinese cabbage, broccoli, and kale. Six isolates of S. sclerotiorum and three isolates of S. minor were tested for their pathogenicity to the crucifers by artificial inoculation. All the isolates of the two Sclerotinia spp. induced rot symptoms on the plants of the crucifers tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility of the crucifers to the isolates of S. sclerotiorum. However, in case of S. minor, radish was relatively less susceptible to the pathogen.

• Research in Plant Disease
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• v.26 no.4
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• pp.256-263
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• 2020

Incidence of Fusarium wilt was surveyed in fields of summer radish in Gangwon province in Korea in 2018 and 2019. The disease started in early July and spread rapidly in hot summer of late July and August and in severe case, reached up to 80% in a field in Gangneung area. Symptoms in the seedling stage include poor growth and browning of internal tissue of root. During mid-growth, the leaves of diseased plant turned yellow over time, the surface of the roots changed from white to blackish, and the vascular tissues turned brown. A total of 23 isolates was obtained from the diseased plants and identified as Fusarium oxysporum f. sp. raphani by elongation factor-1α and intergenic spacer sequence analysis. Pathogenicity of the isolates was tested by artificial inoculation to the radish and other plants. All the isolates tested were pathogenic to radish plant, although there were differences in virulence on radish 11 cultivars. However, the isolates were not virulent to other plants except some cruciferous vegetables including Brussels sprouts, rocket, stock, and turnip. The results of pathogenicity test showed that it is necessary to rotate with crops other than cruciferous vegetables in order to prevent Fusarium wilt from radish fields.

• Journal of Environmental Science International
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• v.16 no.9
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• pp.1027-1037
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• 2007

The purpose of this study was to investigate the flora of Mt. Bongrae in Busan from February 2004 to May 2005. The flora in the surveyed area were identified 291 taxa that belonged to 82 families, 201 genera, 251 species, 35 varieties, and 5 forms. Among them, evergreen broad trees were identified 21 taxa, naturalized plants 30 taxa, especial plants decided by Ministry of Environment 24 taxa. By the vegetation of each slope, in northeast slope, it was dominated such as Pinus thunbergii community, Chamaecyparis obtusa community, Amorhpa fruticosa community, Trachelospermum asiaticum var. intermedium-Hedera rhombea community, north-northeast slope, it was distributed both Pinus thunbergii community and board-leaved tree community. In the southwest slope of coast site, it was presented plantation plants, according to altitude distributed such as natural vegetation, evergreen broad trees, warm-temperate plants. In southeast slope, it was to represent secondary vegetation such as Alnus firma community as a forest fire. Underside was mainly to search herbs such as naturalized plants and cruciferous family as a vegetable garden. In northwest slope, it was searched trees such as Pinus thunbergii community, Lindera obtusiloba, Styrax japonca, Prunus sargentii, Aralia elata, Zanthoxylum piperitum, Akebia quinata and herb layer such as Osmunda japonica, Allium thunbergii, Liriope platyphylla, Corydalis ochotensis, Scilla scilloides, Viola orientalis. Around the top, it was distributed such as Rhododendron mucronulatum-Rhododendron schlippenbachii community, Carpinus coreana, Viburnum erosum, Sorbus alnifolia.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.625-629
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• 1998

Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-poly-merase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 nm in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV--specfic oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var. hebecarpa, Rorippa cantoniensis and Thlaspi arvense.

• Journal of Life Science
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• v.32 no.4
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• pp.298-302
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• 2022

Sulforaphane is a sulfur-containing substance found in large amounts in cruciferous plants and has been reported in several studies to have anticancer effects. Kale is a representative cruciferous plant known as a superfood and is widely used as an ingredient in various dishes. In this study, in order to investigate a cultivation method for increasing kale's content of sulforaphane, kale was treated with geraniol or methyl jasmonate and water stressed during cultivation using a aeroponic culture system in a fully enclosed plant factory. Geraniol or methyl jasmonate were sprayed on the kale's leaf surface once a day for 2 days, and water deprivation stress was conducted for 3 days after 7 days from first treatment day. No difference in growth between control, geraniol, methyl jasmonate treated groups were observed during cultivation. The study results showed that the kale sulforaphane content increased by 60% in the group treated with geraniol compared to the control group and that the group treated with water deprivation stress in addition to geraniol showed a significant increase of 414%. These results show that kale with an increased content of sulforaphane can be grown and that geraniol can be a good research material for increasing the content of functional substances in plants.