Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Status of Fusarium Wilt Incidence on Summer Radish and Etiological Characteristics of the Causal Fungus in Korea

고랭지 여름 무에서 시들음병 발생 현황과 병원균의 병원학적 특성 연구

  • Hong, Sung Kee (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ko, Hyoungrai (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Youngkee (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Jeomsoon (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration)
  • 홍성기 (국립농업과학원 작물보호과) ;
  • 고형래 (국립농업과학원 작물보호과) ;
  • 최효원 (국립농업과학원 작물보호과) ;
  • 이영기 (국립농업과학원 작물보호과) ;
  • 김점순 (국립농업과학원 유해생물팀)
  • Received : 2020.10.04
  • Accepted : 2020.12.09
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Incidence of Fusarium wilt was surveyed in fields of summer radish in Gangwon province in Korea in 2018 and 2019. The disease started in early July and spread rapidly in hot summer of late July and August and in severe case, reached up to 80% in a field in Gangneung area. Symptoms in the seedling stage include poor growth and browning of internal tissue of root. During mid-growth, the leaves of diseased plant turned yellow over time, the surface of the roots changed from white to blackish, and the vascular tissues turned brown. A total of 23 isolates was obtained from the diseased plants and identified as Fusarium oxysporum f. sp. raphani by elongation factor-1α and intergenic spacer sequence analysis. Pathogenicity of the isolates was tested by artificial inoculation to the radish and other plants. All the isolates tested were pathogenic to radish plant, although there were differences in virulence on radish 11 cultivars. However, the isolates were not virulent to other plants except some cruciferous vegetables including Brussels sprouts, rocket, stock, and turnip. The results of pathogenicity test showed that it is necessary to rotate with crops other than cruciferous vegetables in order to prevent Fusarium wilt from radish fields.

2018년과 2019년에 강원도 여름 재배 무 포장에서 시들음병 발생이 조사되었다. 시들음병은 7월 상순 발생했고, 7월 하순과 8월 뜨거운 여름에 급속히 확산되었으며, 심한 경우, 일부 포장에서는 80%까지 발생하였다. 유묘기에 병든 무는 생육이 불량하고 뿌리 내부조직이 갈변되었다. 생육중기 동안 시간이 지나면서 병든 잎은 황화되고 뿌리표면은 흰색에서 흑색으로 변색되고, 도관조직은 갈변하였다. 병든 식물체로부터 23개 Fusarium균이 분리되었고, EF-1α와 IGS 염기서열 분석에 의해 Fusarium oxysporum f. sp. raphani로 동정되었다. 무와 다른 작물에 대한 인공접종에 의해 병원성이 검정되었다. 11개 품종 간에 차이는 있었으나 모든 균주들은 무에서 병원성이었다. 그러나 로켓, 방울양배추, 스토크, 순무를 포함하는 배추과 작물을 제외하고는 병원성이 없었다. 병원성 검정결과는 무 포장에서 시들음병을 방제하기 위해서는 배추과 채소 이외의 작물로 돌려짓기하는 것이 필요하다는 것을 나타낸다.

Keywords

References

  1. Baik, S.-Y., Jang, K. S., Choi, Y. H., Kim, J.-C. and Choi, G. J. 2011. Resistance degree of radish cultivars to Fusarium oxysporum f. sp. raphani according to several conditions. Korean J. Hortic. Sci. Technol. 29: 48-52.
  2. Bosland, P. W. and Williams, P. H. 1987. An evaluation of Fusarium oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility, and geographic origin. Can. J. Bot. 65: 2067-2073. https://doi.org/10.1139/b87-282
  3. Chi, M.-H., Park, S.-Y. and Lee, Y.-H. 2009. A quick and safe method for fungal DNA extraction. Plant Pathol. J. 25: 108-111. https://doi.org/10.5423/PPJ.2009.25.1.108
  4. Enya, J., Togawa, M., Takeuchi, T., Yoshida, S., Tsushima, S., Arie, T. et al. 2008. Biological and phylogenetic characterization of Fusarium oxysporum complex, which causes yellows on Brassica spp., and proposal of F. oxysporum f. sp. rapae, a novel forma specialis pathogenic on B. rapa in Japan. Phytopathology 98: 475-483. https://doi.org/10.1094/PHYTO-98-4-0475
  5. Garibaldi, A., Gilardi, G. and Gullino, M. L. 2006. Evidence for an expanded host range of Fusarium oxysporum f. sp. raphani. Phytoparasitica 34: 115-121. https://doi.org/10.1007/BF02981311
  6. Kim, J.-S., Lee, Y. K., Hong, S. K. and Choi, H.-W. 2019. First report of Verticillium dahliae causing Verticillium wilt on potato in Korea. Plant Dis. 103: 2470.
  7. Korean Society of Plant Pathology. 2009. List of Plant Diseases in Korea. 5th ed. Korean Society of Plant Pathology, Suwon, Korea. 853 pp. (In Korean)
  8. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35: 1547-1549. https://doi.org/10.1093/molbev/msy096
  9. Lee, J. H., Jang, K. S., Choi, Y. H., Kim, H. and Choi, G. J. 2016. Development of an efficient mass-screening method for testing the resistance of radish to Fusarium wilt. Res. Plant Dis. 22: 152-157. (In Korean) https://doi.org/10.5423/RPD.2016.22.3.152
  10. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IW, USA. 388 pp.
  11. Mbofung, G. Y., Hong, S. G. and Pryor, B. M. 2007. Phylogeny of Fusarium oxysporum f. sp. lactucae inferred from mitochondrial small unit, elongation factor-1α and nuclear ribosomal intergenic spacer sequence data. Phtopathology 97: 87-98. https://doi.org/10.1094/PHYTO-97-0087
  12. Moon, Y. G., Kim, W. G., Cho, W. D. and Sung, J. M. 2001. Occurrence of Fusarium wilt on cruciferous vegetable crops and pathogenic differentiation of the causal fungus. Res. Plant Dis. 7: 93-101. (In Korean)
  13. Nelson, P. E., Toussoun, T. A. and Marasas, W. F. O. 1983. Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park, PA, USA. 193 pp.
  14. O'Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Natl. Acad. Sci. U. S. A. 95: 2044-2049. https://doi.org/10.1073/pnas.95.5.2044
  15. Rural Development Administration. 2017. Handbook to Agricultural Technology, 126: Radish. Rural Development Administration, Jeonju, Korea. (In Korean)
  16. Srinivasan, K., Gilardi, G., Spadaro, D., Gullino, M. L. and Garibaldi, A. 2010. Molecular characterization through IGS sequencing of formae speciales of Fusarium oxysporum pathogenic on lamb's lettuce. Phytopathol. Mediterr. 49: 309-320.
  17. Yan, W., Li, B., Chai, A. Shi, Y., Shi, J. and Zhang, X. 2019. First report of Verticillium wilt on cultivated radish caused by Verticillium dahliae Kleb. in China. J. Plant Pathol. 101: 777. https://doi.org/10.1007/s42161-019-00243-4