• Title/Summary/Keyword: Crossing Culture

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Introduction of a speedy growing trait into Pleurotus eryngii by backcrossing (큰느타리(새송이)버섯에서 역교배를 통한 속성형질의 도입)

  • Im, Chak-Han;Kim, Min-Keun;Je, Hee Jung;Kim, Kyung-Hee;Ryu, Jae-San
    • Journal of Mushroom
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    • v.10 no.2
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    • pp.49-56
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    • 2012
  • Two strains which have good traits in quality and speedy growing were selected based on previous report(Ryu, 2007) to breed a new strain carrying the traits in it. KNR2322 carrying high quality and KNR2503 carrying a speedy growing trait were cultivated to harvest monokaryons. The monokaryotic mycelia were characterized in growth rate, color, and stability. Selfing of monokaryons from KNR2322 and crossing between KNR2322- and KNR2503-derived monokaryons were performed. Two parental lines, $KNR2322-4{\times}37-6{\times}12(B)$ showing thremmatological values in weight and quality, 68.5g and 6.5 and $KNR2322-30{\times}KNR2503-60(A)$ was harvested in 13.5 days after scraping old medium were selected. Crossing between A and B resulted in two high quality and speedy growing strain, A8B8 and A8B10. Their days for harvest after scraping, weight, and quality were 14.0 days, 88.8g and 92.7g, 7.0 and 7.1. Originality tests were conducted with genomic DNAs of A8B8 and Keuneutari 3ho(commercial strain) by DNA fingerprinting a confrontation culture. The results showed polymorphism and a inhibition line between them. It suggested that the new strain have originality from the commercial strain. A8B8 have been registered in Korea Seed and Variety Service with as "Saesongi 1ho".

Breeding of Doritaenopsis 'Hwasu 5205' with Vivid Red and Large Flowers (선명한 적색 대륜계 호접란 '화수 5205' 육성)

  • Lim, Ki-Byung;Kim, Hong-Yul;Park, No-Eun;Son, Beung-Gu;Yun, Suk-Young
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.941-946
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    • 2015
  • A new Doritaenopsis cultivar 'Hwasu 5205' was bred by Kyungpook National University, Korea, which produces young plants through tissue culture techniques. The new cultivar 'Hwasu 5205', showing the phenotype of vivid red and large flower type characteristics, was derived from crossing between Phalaenopsis Happy Valentine and Doritaenopsis Happy Rose. An elite individual, number '02-05-205' later named as 'Hwasu 5205', was selected among about 300 individual progenies after more than 2 years of intensive selection covering vegetative and flowering distinctiveness. In year 2004-2005, 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'Hwasu 5205' produces vivid red (RHS #PN78B) flowers of i ncurved type with large size, of 9.2 and 12.0 cm in flower height and width, respectively. Leaves of 'Hwasu 5205' grow horizontally and are about 24.3cm in length and 8.5cm in width, respectively. This cultivar possesses no genetic variation. It can be propagated rapidly in vitro and is easy to grow due to its vigorous growth habit. 'Hwasu 5205' was registered (Reg. #: 2915) to Korea Seed & Variety Service (KSVS) on 1st December, 2009 and the PBR(plant breeder's right)is currently controlled by Sangmiwon Orchid Company, Korea.

Induction of A Chromosome-doubled Persimmon (Diospyros kaki Thunb.) by in vitro Colchicine Treatment (기내 콜히친 처리에 의한 염색체 배가 감 식물체 유기)

  • Ma, Kyeong-Bok;Cho, Kwang-Sik;Jung, Hae-Won;Seo, Ho-Jin;Kang, Sam-Seok
    • Korean Journal of Plant Resources
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    • v.31 no.5
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    • pp.515-521
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    • 2018
  • This was carried out to develop a chromosome-doubled (12x) persimmon that will be used as a crossing parent to select seedless persimmon cultivars with the change of the consumption trend recently. To obtain a chromosome-doubled (12x) persimmon, colchicine was applied at the meristem of seedlings in vitro derived from cross among hexaploid persimmon (Diopyros kaki Thunb.). These were treated with 0.03%, 0.05% and 0.1% colchicine respectively for doubling chromosome, and it was most effective at the concentration of 0.05% colchicine. After colchicine treatment, we conducted tests to elucidate conditions for inducing shoot and root development. As the result, the shoots grew best when cultivated at 1/2MS media plus 10 and $30{\mu}M$ zeatin respectively, and the roots grew best when cultivated at 1/2MS media after dipping for 5 seconds at 10 mM NAA+5% DMSO. We also compared seedlings that have chromosome (6x) do not doubled and crossing parents (6x) and chromosome-doubled seedlings (12x). As the result, these chromosome-doubled seedlings (12x) showed lower stomatal density and larger stomatal size.

Stain improvement in the white button mushroom 'Seolgang' and its varietal characteristics in Agaricus bisporus

  • Lee, Byung-Joo;Lee, Mi-Ae;Kim, Yong-Gyun;Lee, Kwang-Won;Lim, Yong-Pyo;Lee, Byung-Eui;Song, Ho-Yeon
    • Journal of Mushroom
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    • v.10 no.4
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    • pp.151-159
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    • 2012
  • The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, 'Seolgang' was developed by crossing two monokaryons 'CM020913-27' and 'SSU423-31'. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of 'Seolgang' on CDA was better at $20^{\circ}C$ and $25^{\circ}C$ when it was compared with that of '505 Ho'. The mature cap shape of new strain 'Seolgang' is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain '505 Ho', the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of '505 Ho'. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of '505 Ho'. Genetic analysis of the new strain 'Seolgang' showed different profiles compared to '505 Ho', CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

Activation plans of the Cultural Exchanges between Korea' and the Czech Republic's Animation (한국과 체코의 애니메이션 교류를 통한 문화교류 활성화 연구)

  • Park, Ki Bok
    • Cartoon and Animation Studies
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    • s.36
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    • pp.619-641
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    • 2014
  • Animation is an important tool to establish the identity of mutually related countries, as cultural contents with the economic value. That is, it is necessary that the Korean animation should extend the previous boundary of exchange, for the status of representative cultural industry in the 21st century global market. Especially, to overcome the regionally prejudiced and unbalanced cultural exchange, the possibility of extension with Czech animation, which has not had an active exchange with Korea, will be considered and the efficient method will be proposed. The Czech Republic is geopolitically located in the center of Europe and its infrastructure like industrial construction, investment environment, human resources is outstanding among Visegrad Group. The Czech Republic has a remarkable cultural border-crossing phenomenon, and as a complex country of cultural and religious characteristics of eastern and western European, it is a powerful nation leading Central Europe. Therefore, the animation exchange of Czech and Korea is valuable in culture and economy of both nations. Through such an animation exchange of both nations, not only Korean animation secures the diversity of exchange but also insignificant cultural exchange after formal establishment of diplomatic relations in 1990, will be revitalized and international firm relationship of both nations will be established. Therefore, as an activation plan or cultural exchange of Korea and the Czech Republic, concrete plan like Stop Motion Animation Workshop is proposed.

Immature Ovule Culture of Lilium Oriental Interspecific crossings (오리엔탈 종간잡종 나리 미숙배주 배양)

  • Choi, Eun Gyung;Park, Byung Mo
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.221-225
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    • 2009
  • Interspecific crossing was conducted to obtain hybrid lilly with better quality of adapting to the unfavorable environment using oriental Lilium spp. as a female parent. Average interspecific crossing rate between oriental and asiatic lines by cut-style pollination was as low as 29%. Although the average germination rate of zygotic hybrid embryo between oriental 'Rodolfa' ${\times}$ asiatic 'Toronto' was increased from 76.6% to 78.3%. on 114 or 1/6 strength MS medium compared to the control, the rate of zygotic embryos formation between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' was significantly enhanced up about 86~90% on 1/2 strength MS medium. Meanwhile, germination rate of interspecific hybrid embryo between oriental 'Snow Cristal' ${\times}$ asiatic 'Royal Trinity' showed up 100%. Germination rate of interspecific hybrids slightly increased by addition of $0.5-1.0mg{\cdot}L^{-1}$ GA. Germination rate of zygotic embryos between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' and oriental 'Belcanto' ${\times}$ L. callosum was highest on the MS medium containing $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ kinetin, as 66.6% and 45.2% respectively.

A New Early Flowering, Spray Chrysanthemum Cultivar for Cut Flower, "Pinky" with Single Type and Pink Petals (조기개화성의 절화용 핑크색 흩꽃 스프레이국화 "핑키" 육성)

  • Hwang, Ju-Chean;Chin, Young-Don;Chung, Yong-Mo;Kim, Su-Gyeong
    • Korean Journal of Breeding Science
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    • v.40 no.2
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    • pp.196-200
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    • 2008
  • A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Pinky' was bred from a cross between 'Rima' and 'Yen'. Progenies were investigated from 2004 to 2006 under shading culture in spring and retarding culture in autumn and the promising line was selected based on the performance of year-round production, white rust resistance and vase life. Pinky flowered around 20 October, and year-round production was possible by shading and lighting. Its flower was 3.1 cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. Days to flower from short-day start for 'Pinky' was about 47 in spring. 'Pinky' showed a vase life of 20.9 days in autumn. This cultivar was commercially registered in 2007. 'Pinky' would be suitable for culture under the greenhouse and rain shelter condition in Korea.

A Study on the Color comparison of Korean Saek-dong and Italian Futurist Fashion (한국의 색동과 이탈리아 미래주의 복식의 색채 비교 연구)

  • 이금희
    • Journal of the Korean Home Economics Association
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    • v.41 no.8
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    • pp.33-53
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    • 2003
  • A Study on the color comparison of Korean Saek-dong and Italian futurist fashion It is generally recognized that the color scheme and its characteristics as a product of living culture are strongly reflected in clothing. This study concentrates on the color comparison of Korean Saek-dong which has been used in traditional Korean clothing and Italian futurist fashion which showed dynamic characteristics and brilliant colors. The purpose of this study is to investigate the external format, emotions, functions and meanings of the colors in Korean Saek-dong and Futurist fashion, and to find similarities and differences between them. The results of the study are as follows. The similarities between them are harmony of vivid colors like a rainbow, no-use of black color and expression of rhythm through repeated geometrical shapes. They have been used as festive costumes and have implied meaning of happiness and pleasure. The differences, in the external formats, are as follows. Korean Saek-dong has striped patterns including the color white, and has regular distances among the stripes. But, Futurist fashion includes luminous or fluorescent colors and metallic colors. In addition, it has repeated motifs of geometrical shapes and geometrically divided areas. While Saek-dong expresses Sangsaeng through the conceptual use of color, Futurist fashion shows simultaneity, speed and dynamics through spiritual functions of crossing and inter-penetration. In emotional aspect, Saek-dong expresses pleasure of children's mind and Futurist fashion expresses pleasure of city. In functional aspect, Saek-dons expresses a concept of ceremonial beauty, which is spiritual and symbolizes good auspices, holiness and sorcery. So it is used for ceremonial costume. But, Futurist fashion reflects the harmony of colors created from modem and urban images and shows the willingness and emotion of solving futuristic avant-garde, modernity, dynamics, transformation and bellicosity. So it is used for daywear. While Saek-dong represents succession of tradition, Futurist fashion represents resistance of tradition in cultural aspects.

The Present and the Future Issues in Korean Feminist Theatre (한국의 페미니즘 연극, 그 현황과 과제)

  • 최영주
    • Lingua Humanitatis
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    • v.6
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    • pp.359-380
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    • 2004
  • Considering Korean feminist theatre is not successful at all these days, it is needed to find the reasons of its crisis. As two Korean feminist theatre Scholars argued, the crisis owed to the structural problem from the start. That is the Korean feminist theatre started and developed depending on the imported popular feminist plays without social and cultural self-consciousness. Once the imported feminist theatres were flourished, some theatre companies pursued the commercial success blurring the feminist issues. It was resulted into the intentional ignorance in and out of the theatre society. While, some feminist plays were too inclined to the agit-prop without artistic sophistication. This essay tries to examine how the feminist theatres have been developing and what kinds of feminist performances have been made until now. And it intends to emphasize that the play text should be based on the Korean women's past, present, and future reality. Besides, they have to delve into the problem by which the Korean women were trapped in historical, social, and cultural environment. To make the women's matter the social issue at present and fur the future, the Korean feminist theatre should re-find its place as the socio-cultural forum. First, Korean theatre should cooperate with the other women's group crossing the different disciplines of the society, the culture, the politics etc . Secondly, we need to observe and watch where and how the distortion happens in women's matter, and react to correct it. Thirdly, we need to discover, to support, and to protect the women centered perspective of some playwrights as well as the performers. Co-writing or co-performing is also very positive to diversify the women's subjects. Lastly, to protect the feminist theatre against the consumerism, they need to have the financial support from the government or some civil society.

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Physio-chemical impacts on efficient germination in Platycodon grandiflorum for. duplex pollen culture

  • Kwon, Soo Jeong;Hwang, Ha Nule;Moon, Young Ja;Cho, Gab Yeon;Woo, Sun Hee;Boo, Hee Ock;Koo, Jin-Woog;Kim, Hag Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.170-170
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    • 2017
  • The highly valued ornamental plant, Platycodon grandiflorum for. duplex was generated by petaloidy of a calyx of Platycodon grandiflorum. The present investigation was executed to explore the several factors having effects on the germination of pollens with a view to acquire the underlying data for the artificial crossing to cultivate the species of Platycodon grandiflorum for. duplex. Both low and high temperature impaired the germination of Platycodon grandiflorum for. duplex pollens. The good germination rate was observed at the temperature of $25^{\circ}C$. The types and concentrations of carbon sources induced the differences in germination rate. The germination rate increased with the increasing concentration of sucrose and glucose except for fructose. Sucrose and glucose showed the highest results at the concentration of 20%. While fructose demonstrated the similar tendency to sucrose and glucose, it reduced the germination rate at the concentration of 20%. The highest germination rate was observed at the concentration of 15%. The appropriate carbon course for germination of pollens of Platycodon grandiflorum for. duplex was glucose of which germination rate was twice as high as that of sucrose and fructose. The germination rate was reduced substantially when the pH was close to alkali, and the potential germination rate was obtained at pH 6. Boric acid enhanced the germination rate at a lower concentration than the higher concentration.

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