• Asian-Australasian Journal of Animal Sciences
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• v.24 no.1
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• pp.82-87
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• 2011

A study was conducted to determine the relationships between triacylglycerol (TAG) of plasma, very low density lipoprotein (VLDL) and fat deposition in two different breeds of chickens. The VLDL apolipoproteins of both breeds were also characterised. The breeds used were crossbred village chicken (AK) (Sasso crossed) and commercial broiler (CB) (Avian). They were housed in six pens with 30 female and 30 male birds of each breed per pen. Three male and three female birds from each pen were slaughtered and the blood was collected. The VLDL was isolated and sub-fractionated using Fast Protein Liquid Chromatography (FPLC). VLDL TAG of CB was significantly lower than AK. The particle size was negatively correlated with VLDL TAG and positively correlated with abdominal fat. Sub-fraction 2 contained more apo E that will enhance the lipolysis process of the VLDL TAG than subfraction 1. CB had a higher proportion of sub-fraction 2 than AK. The results showed that the proportion of sub-fraction 2 was negatively correlated with VLDL TAG concentration and positively correlated with abdominal fat.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.991-997
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• 2019

This study was conducted to investigate the laying period performance of two crossbred Korean native chicken (KNC) grand parent stock (GPS) layer during 20 to 40 weeks. A total of 351 20-week-old KNC GPS were allocated in a completely randomized design to give 4 birds per each cage. The chickens were fed commercial diets [i.e., week 20 - 32, crude protein (CP) 18.0% and metabolizable energy (ME) 2,850 kcal/kg; week 32 - 40, CP 17.0% and ME 2,800 kcal/kg)] and fresh water during the whole experiment period ad libitum basis. Body weight, age of sexual maturity, egg weight, hen-day egg production (HDP) and feed conversion ratio (FCR) were measured during the experiment period (week 20 - 40). The results show no significant difference in the Body weight, age of sexual maturity, egg weight, and hen-day egg production (HDP) except for egg weight. 1B had a higher egg weight on week 36 only. We expect that the reason why most factors showed no significant difference is the lack of nutrients caused by limited feeding which follows the standard feeding system for the grand-parent stock. These results provide basic information for laying data in 2-way crossbred Korean native chickens.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.1033-1040
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• 2022

The current experiment was conducted to evaluate the effect of diallel crossbreeding on the body weight and laying performance of Korean native chicken from hatch to week 40. A total of 1,000 one-day-old chicks were allotted to 10 cages per crossbreeding treatment with 10 birds per cage on a random basis and then raised until 16 weeks and subsequently moved to layer battery cages and raised until 40 weeks. Ten crossbred treatments (YC, YD, YF, YK, CD, CF, CK, DF, DK, FK) that were obtained from the diallel crossbred of five pure lines were used in the current experiment. The body weight and mortality were measured biweekly from hatching to week 20 and every four weeks from week 20 to week 40. The number of eggs was measured daily. YC and YD crossbreeds showed a higher body weight (p < 0.05) and FK crossbreed showed a lower body weight (p < 0.05) during the whole xperimental period. The week 20 weight range was 1,501 to 1,729 g and the week 40 weight range was from 1,829 to 2,179 g. Earlier onset of egg-laying was noted in the YC and YD groups whereas late onset was observed for the DF and DK groups. YK reached its peak earliest at week 25 with 89.15% while the DK crossbreed attained its peak at week 36 with 89.69%. The YC and YD crossbreeds showed the improved body and egg-laying performance. Conclusively, there are variations in the body weight and laying performance of Korean native chickens with diallel crossing.

• Korean Journal of Poultry Science
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• v.39 no.4
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• pp.311-315
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• 2012

The increasing demand for Korean native chicken meat indicates that the discovery of haplotypes is very important from both economic and conservation points of view. In this study, mtDNA D-loop sequences from two crossbred Korean native chicken populations of 138 individuals were investigated. Twenty six nucleotide substitutions were identified from sequence analysis and were classified into 12 haplotypes. The haplotype H_8 represents 73.47% of Woorimatdag (chicken population) sequences, which were identified in all five Woorimatdag chicken populations investigated. The H_7 haplotype (Dhap1) for D population covers 45% sequences, which indicate maternal inheritance from black Korean native chicken. On the other hand, Chap3 and Chap4 for C population are specific haplotypes, as H_5 and H_2, respectively. Based on the network profiles, six SNPs (C199T, A239G, G242A, A291G, T330C and C391A) of the D-loop region are effective markers for discrimination between Woorimatdag and Hanhyup chicken populations. Also, the phylogenetic analyses of Woorimatdag and Hanhyup chicken populations were used to identify the genetic relationships among the haplotypes. The results presented here can be used for developing molecular markers to discriminate between two commercial Korean native chickens.

• Korean Journal of Poultry Science
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• v.38 no.4
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• pp.293-304
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• 2011

The current work was carried out to investigate the effect of crossbred Korean native chickens (KNC) on performance and meat quality. A total of 720 chicks (1d of age) was used in this work and were divided into groups by crossbreds (A, B, C and D) and sex (male and female). Crossbreds were A) (KNC egg-meat type C strains ${\times}$ KNC meat type S strains) ${\times}$ Ross broiler, B) (KNC egg-meat type C strains ${\times}$ KNC meat type H strains) ${\times}$ KNC meat type S strains, C) (KNC native R strains${\times}$KNC meat type S strains)${\times}$KNC meat type H strains and D(White Semibroiler Chickens). Experimental diets consisted of 3 phases such as starter (0~5 weeks; CP 20.0%, ME 3,050 kcal/kg), earlier (5~8 weeks; CP 18.0%, ME 3,100 kcal/kg) and finisher (8~12 weeks; CP 16.0%, ME 3,150 kcal/kg). They were fed the broiler diets for 12 weeks at the flat house and thirty six chickens were slaughtered at week 5 and 10. There was no significant difference on the fertility of crossbred KNCs, and the hatchability of B crossbred was low compared to other crossbreds. On body weight (BW), D crossbred resulted in a higher BW after 5 weeks (P<0.05). Body weight gain (BWG) and feed intake (FI) of A crossbred were also significant higher compared to the other crossbreds for all periods. On carcass ratio (CR), A crossbred showed higher CR at 5 weeks, there was no significant among crossbreds at 10 weeks. The partial meat ratio(ala, breast, neck, leg) of C crossbred was the lowest among other crossbreds (P<0.05), but the back meat ratio was not difference among treatments at 5 and 10 weeks. pH of meats have no difference among crossbreds at 5 week, and A crossbred was high compared to other crossbreds at 10 week (P<0.05). The moisture content of D crossbred meat and the protein content of B crossbred meat were high compared to other crossbreds at 5 week. Chemical compositions of meats have no difference among crossbreds at 10 week. Lightness and redness have not significance among crossbreds at 5 week, and redness of A crossbred was the highest among all crossbreds (P<0.05). Shear force and cooking loss of A crossbred were high at 5 week, and cooking loss of A crossbred was low compared with other crossbreds at 10 week. These results suggested the basic data that needed to develope the new strains.

• Korean Journal of Poultry Science
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• v.49 no.3
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• pp.157-165
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• 2022

Chickens are a species of vertebrate with varying colors. Various colors of chickens must be classified to find color-related genes. In the past, color scoring was performed based on human visual observation. Therefore, chicken colors have not been measured with precise standards. In order to solve this problem, a computer vision approach was used in this study. Image quantization based on k-means clustering for all pixels of RGB values can objectively distinguish inherited colors that are expressed in various ways. This study was also conducted to determine whether plumage color differences exist in the reciprocal cross lines between two breeds: black Yeonsan Ogye (YO) and White Leghorn (WL). Line B is a crossbred line between YO males and WL females while Line L is a reciprocal crossbred line between WL males and YO females. One male and ten females were selected for each F₁ line, and full-sib mating was conducted to generate 883 F₂ birds. The results indicate that the distribution of light and dark colors of k-means clustering converged to 7:3. Additionally, the color of Line B was lighter than that of Line L (P<0.01). This study suggests that the genes underlying plumage colors can be identified using quantification values from the computer vision approach described in this study.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.735-747
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• 2023

The composition of fatty acids determines the flavor and quality of meat. Flavor compounds are generated during the cooking process by the decomposition of volatile fatty acids via lipid oxidation. A number of research on candidate genes related to fatty acid content in livestock species have been published. The majority of these studies focused on pigs and cattle; the association between fatty acid composition and meat quality in chickens has rarely been reported. Therefore, this study investigated candidate genes associated with fatty acid composition in chickens. A genome-wide association study (GWAS) was performed on 767 individuals from an F2 crossbred population of Yeonsan Ogye and White Leghorn chickens. The Illumina chicken 60K significant single-nucleotide polymorphism (SNP) genotype data and 30 fatty acids (%) in the breast meat of animals slaughtered at 10 weeks of age were analyzed. SNPs were shown to be significant in 15 traits: C10:0, C14:0, C18:0, C18:1n-7, C18:1n-9, C18:2n-6, C20:0, C20:2, C20:3n-6, C20:4n-6, C20:5n-3, C24:0, C24:1n-9, monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). These SNPs were mostly located on chromosome 10 and around the following genes: ACSS3, BTG1, MCEE, PPARGC1A, ACSL4, ELOVL4, CYB5R4, ME1, and TRPM1. Both oleic acid and arachidonic acid contained the candidate genes: MCEE and TRPM1. These two fatty acids are antagonistic to each other and have been identified as traits that contribute to the production of volatile fatty acids. The results of this study improve our understanding of the genetic mechanisms through which fatty acids in chicken affect the meat flavor.

• Korean Journal of Agricultural Science
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• v.45 no.4
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• pp.733-739
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• 2018

This study was conducted to investigate the growth performance between six different Korean crossbred native chickens. A total of 312 one-day-old male chickens from the same paternal line with six different maternal lines were allocated into six groups (1A, 2A, 3A, 4A, 5A, and 6A) to give four replicate cages (13 birds per cage). Corn soybean meal based diets were provided as a starter (0 - 5 weeks: 20% crude protein [CP] and 3,050 kcal/kg metabolizable energy [ME]), grower (5 - 8 weeks: 18% CP, 3 and 100 kcal/kg ME) and finisher (8 - 12 weeks: 17% CP and 3,150 kcal/kg ME) diet. Chickens were able to access their respective feed and water ad libitum. In week 2, 2A had a lower body weight (p < 0.05) than that of the other groups. In contrast, in weeks 4 and 8, 2A had a higher body weight (p < 0.05). Similarly, in week 6, 2A had a higher body weight (p = 0.76) than that of the others. However, no difference (p > 0.05) was observed in the body weight after week 10. Meanwhile, 2A had a lower feed conversion ratio (FCR) than that of the other groups (p < 0.05) and a higher average daily feed intake (ADFI; p < 0.05) compared to the other groups in week 6. In conclusion, crossbred 2A had a lower growth performance in week 2; however, in weeks 4 and 6, 2A had a higher growth performance than that of the other groups. Based on this conclusion, we can use those results as a basis for further study.

• Korean Journal of Poultry Science
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• v.38 no.4
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• pp.331-338
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• 2011

The study was conducted to evaluate the production and the performance test of egg-type commercial Korean Native Chickens. A total of 360 female layers were produced from CY${\times}$C (A), CL${\times}$C (B), CG${\times}$C (C) and CW${\times}$C (D) crossbreds kept at National Institute of Animal Science, Korea, and breed during 20~64 weeks to investigate the productivity and the performance. Layers were divided into 4 crossbreds (6 replications/crossbred, 15 heads/replication). Age and egg weight at first egg was no significantly different among crossbreds. Body weight (BW) at first egg of D crossbred was significantly lower compared to that of other crossbreds (P<0.05). Weekly BW of B crossbred was the highest at 20, 30 and 40 weeks of age, but BW of D crossbred was the lowest among crossbreds. Feed intake (FI) of B crossbred was significantly higher compared to the other crossbreds at 20~24, 32~44 and 52~60 weeks of age. FI of D crossbred was significantly lower compared to other crossbreds after the age of 44 weeks (P<0.05). Average egg weight of B crossbred was significantly higher than the other crossbreds at the age of 24~60 weeks (P<0.05). Egg production ratio (%) of C crossbred was significantly higher compared to the other crossbreds for whole test periods (P<0.05). Hen-housed egg production number at 64 weeks of age for A, B, C and D crossbreds resulted in 241.4, 235.6, 232.3 and 227.0, respectively. Feed conversion ratio of A and C crossbreds was lower compared to the other crossbreds at the age of 20~24 weeks and C crossbred was the lowest among the crossbreds at the age of 60~64 weeks. These results provide the basic data which will be helpful to develope the new strains of commercial Korean Native Chickens.

• Korean Journal of Poultry Science
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• v.37 no.4
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• pp.399-404
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• 2010

This work was conducted to evaluate the hatching and growing performance of Korean native chicken (KNC) 3 strains crossbred. A total of 360 female pullets were produced from $\times$ (A), CL $\times$ C (B), CG $\times$ C (C) and CW $\times$ C (D) crossbred kept in National Institute of Animal Science, Korea. Hatchability of A, B, C and D were 69.8, 70.7, 75.5 and 77.4%, respectively. Livability were not significantly different by 20 weeks among 4 strains. Body weight B was high compared to other strains at 20 weeks. The feed conversion ratio of 4 strains was no significantly different among strains at 20 weeks. These results suggested the basic data that needed to develope the new strains.