• Molecules and Cells
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• 제39권12호
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• pp.862-868
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• 2016

Goats (Capra hircus) are one of the oldest species of domesticated animals. Native Korean goats are a particularly interesting group, as they are indigenous to the area and were raised in the Korean peninsula almost 2,000 years ago. Although they have a small body size and produce low volumes of milk and meat, they are quite resistant to lumbar paralysis. Our study aimed to reveal the distinct genetic features and patterns of selection in native Korean goats by comparing the genomes of native Korean goat and crossbred goat populations. We sequenced the whole genome of 15 native Korean goats and 11 crossbred goats using next-generation sequencing (Illumina platform) to compare the genomes of the two populations. We found decreased nucleotide diversity in the native Korean goats compared to the crossbred goats. Genetic structural analysis demonstrated that the native Korean goat and cross-bred goat populations shared a common ancestry, but were clearly distinct. Finally, to reveal the native Korean goat's selective sweep region, selective sweep signals were identified in the native Korean goat genome using cross-population extended haplotype homozygosity (XP-EHH) and a cross-population composite likelihood ratio test (XP-CLR). As a result, we were able to identify candidate genes for recent selection, such as the CCR3 gene, which is related to lumbar paralysis resistance. Combined with future studies and recent goat genome information, this study will contribute to a thorough understanding of the native Korean goat genome.

• Archives of Plastic Surgery
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• 제35권1호
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• pp.7-12
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• 2008

Purpose: Since the report of artificial dermis manufacturing method using collagen by Yannas in 1980, collagen has been effectively used as dermal substitute with its merits such as, lower antigeneicity, controllable biodegradation rate, and minimal inflammatory cytotoxic properties in the dermal tissue engineering field. However, weak mechanical durability was the main drawback of collagen dermal substitute. To improve its stability, mechanical or chemical cross-linking was used. Despite of such process, its clinical use was restricted due to weak durability. To improve the durability of collagen matrix, we designed elastin-incorporated collagen matrix and compared its durability with conventional collagen matrix. Methods: 15mm diameter with 4mm thick collagen dermal matrix was made according to Yannas protocol by mixing 0.5% bovine collagen and chondroitin-6-sulfate followed by degassing, freeze drying, dehydrodermal cross-linking and chemical cross-linking procedure. In elastin incorporated collagen matrix, same procedure was performed by mixing elastin to previous collagen matrix in 4:1 ratio(collagen 80% elastin 20%). In comparison of the two dermal matrix in vitro tests, matrix contracture rate, strain, tensile strength, was measured and stiffness was calculated from comparative analysis. Results: In terms of matrix contracture, the elastin-incorperated added collagen dermis matrix showed 1.2 times more contraction compared to conventional collagen matrix. However, tensile strength showed 1.6 times and stiffness showed 1.6 times increase in elastin-incorporated matrix. Conclusion: Elastin incorperated collagen matrix manufactured by our team showed increased durability due to improvement in tensile strength and stiffness compared to previous collagen matrix($Integra^{(R)}$).

• 한국전자거래학회지
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• 제17권2호
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• pp.115-127
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• 2012

본 연구에서는 국가 간 이동통신서비스의 확산과정에서 국가 간 영향에 의해 한 국가에서의 확산성과가 다른 국가의 확산에 주는 영향을 분석하였다. 그동안 대부분의 이동통신서비스 확산에 대한 국가 간 비교연구들은 주로 국가별로 추정된 확산과정에 대해 사후적인 비교 분석이 일반적인 것에 반하여, 본 연구에서는 최근의 이동통신서비스의 확산이 국경을 넘어 영향을 미치고 있는 현실을 보다 적절히 설명할 수 있다. 본 연구에서는 이를 위하여 다국가 확산모형을 이용하여 한국과 중국, 미국, 일본, 홍콩 등 5개국에서의 이동통신서비스의 확산 과정을 분석하였다. 분석 결과 중국의 이동통신서비스의 확산은 한국, 미국, 일본, 홍콩 등 주변 국가들에서의 이동통신서비스의 확산에 유의한 영향을 받은 것으로 밝혀졌다.

• Journal of Radiation Protection and Research
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• 제41권4호
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• pp.328-332
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• 2016

Background: Boron Neutron Capture Therapy (BNCT) is a new radiation therapy. In BNCT, there exists some very critical problems that should be solved. One of the severest problems is that the treatment effect cannot be known during BNCT in real time. We are now developing a SPECT (single photon emission computed tomography) system (BNCT-SPECT), with a cadmium telluride (CdTe) semiconductor detector. BNCT-SPECT can obtain the BNCT treatment effect by measuring 478 keV gamma-rays emitted from the excited state of $^7Li$ nucleus created by $^{10}B(n,{\alpha})$ $^7Li$ reaction. In the previous studies, we investigated the feasibility of the BNCT-SPECT system. As a result, the S/N ratio did not meet the criterion of S/N > 1 because deterioration of the S/N ratio occurred caused by the influence of Compton scattering especially due to capture gamma-rays of hydrogen. Materials and Methods: We thus produced an arrayed detector with two CdTe crystals to test cross talk phenomenon and to examine an anti-coincidence detection possibility. For more precise analysis for the anti-coincidence detection, we designed and made a collimator having a similar performance to the real BNCT-SPECT. Results and Discussion: We carried out experiments with the collimator to examine the effect of cross talk of scattering gamma-rays between CdTe elements more practically. As a result of measurement the coincidence events were successfully extracted. Conclusion: We are now planning to carry out evaluation of coincidence rate from the measurement and comparison of it with the numerical calculations.

• 전기전자학회논문지
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• 제16권4호
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• pp.328-334
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• 2012

본 논문에서는 레이더 단면적(RCS, Radar Cross Section)을 감소시키기 위한 전자파 구조로서 십자형 다이폴 슬롯을 원소로 가지며 다른 배열형태 구조를 갖는 구형 주파수 선택적 표면(FSS, Frequency Selective Surface) 구조의 전파특성을 해석하였다. 유한크기와 곡면형상을 갖는 구형 주파수 선택적 표면 구조의 주파수 특성을 해석하기 위해 RWG 함수를 적용한 3차원 모멘트법을 사용하였으며 반복법 중 하나인 BiCGSTab(Biconjugate Gradient Stabilized) 알고리즘을 적용하여 해석시간의 효율성을 개선하였다. 1m 직경을 갖는 완전도체(PEC, Perfect electric conductor)의 구에 대한 이론적 해석결과인 Mie의 RCS 특성과 비교 검증하여 제안한 해석 알고리즘의 유효함을 입증하였다. 구형 주파수 선택적 표면 구조에서 슬롯 성분의 배열 방법에 따른 RCS 변화를 관찰하여 비교하였으며, 곡면형상의 주파수 선택구조 설계시 배열의 방법도 RCS 특성에 중요한 변수가 될 수 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1317-1325
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• 2008

Viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. One potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. In order to increase the viral safety of human antihemophilic factor IX, particularly in regard to non-enveloped viruses, a virus removal process using a polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. The most critical factor affecting the filtration efficiency was operating pH and the optimum pH was 6 or 7. Flow rate increased with increasing operating pressure and temperature. Recovery yield in the optimized production-scale process was 96%. No substantial changes were observed in the physical and biochemical characteristics of the filtered factor IX in comparison with those before filtration. A 47-mm disk membrane filter was used to simulate the process performance of the production-scale cartridges and to test if it could remove several experimental model viruses for human pathogenic viruses, including human hepatitis A virus (HAV), porcine parvovirus (PPV), murine encephalomyocarditis virus (EMCV), human immunodeficiency virus type 1 (HIV), bovine viral diarrhea virus (BVDV), and bovine herpes virus (BHV). Non-enveloped viruses (HAV, PPV, and EMCV) as well as enveloped viruses (HIV, BVDV, and BHV) were completely removed during filtration. The log reduction factors achieved were $\geq$6.12 for HAV, $\geq$4.28 for PPV, $\geq$5.33 for EMCV, $\geq$5.51 for HIV, $\geq$5.17 for BVDV, and $\geq$5.75 for BHV. These results indicate that the virus filtration process successfully improved the viral safety of factor IX.

• Annals of Laboratory Medicine
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• 제38권6호
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• pp.530-537
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• 2018

Background: Measurement of insulin and C-peptide concentrations is important for deciding whether insulin treatment is required in diabetic patients. We aimed to investigate the analytical performance of insulin and C-peptide assays using the Lumipulse G1200 system (Fujirebio Inc., Tokyo, Japan). Methods: We examined the precision, linearity, and cross-reactivity of insulin and C-peptide using five insulin analogues and purified proinsulin. A method comparison was conducted between the Lumipulse G1200 and Roche E170 (Roche Diagnostics, Mannheim, Germany) systems in 200 diabetic patients on insulin treatment. Reference intervals for insulin and C-peptide concentrations were determined in 279 healthy individuals. Results: For insulin and C-peptide assays, within-laboratory precision (% CV) was 3.78-4.14 and 2.89-3.35%, respectively. The linearity of the insulin assay in the range of 0-2,778 pmol/L was $R^2=0.9997$, and that of the C-peptide assay in the range of 0-10 nmol/L was $R^2=0.9996$. The correlation coefficient (r) between the Roche E170 and Lumipulse G1200 results was 0.943 (P <0.001) for insulin and 0.996 (P <0.001) for C-peptide. The mean differences in insulin and C-peptide between Lumipulse G1200 and the Roche E170 were 19.4 pmol/L and 0.2 nmol/L, respectively. None of the insulin analogues or proinsulin showed significant cross-reactivity with the Lumipulse G1200. Reference intervals of insulin and C-peptide were 7.64-70.14 pmol/L and 0.17-0.85 nmol/L, respectively. Conclusions: Insulin and C-peptide tests on the Lumipulse G1200 show adequate analytical performance and are expected to be acceptable for use in clinical areas.

• 대한원격탐사학회지
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• 제26권2호
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• pp.209-220
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• 2010

해양현상을 이해하기 위한 관측분야의 노력 중에서 해류 정보의 생산은 가장 어려운 작업 중의 하나이다. 이를 극복하기 위한 대안으로서 연속 화상 자료로부터 해류벡터를 추정하려는 많은 연구들이 진행 되고 있다. 본 연구에서는 한반도 주변의 SeaWiFS (Sea-viewing Wide Field-of-view Sensor) chlorophyll-a 해색 자료와 AVHRR/SST 를 이용하여 연속 화상 사이의 유사한 형태를 추적하는 최대상 관계수법을 사용한 표층 유속 벡터의 추정을 시도하였다. 한국의 남해역에서 적용한 유속 벡터 결과는 해면 고도계를 이용한 지형류, ADCP 관측 결과와 비교하여 유속은 약 15% 정도 작고, 유향은 약 $36^{\circ}$의 차이로 근접하여 기존 연구 결과에 비해 양호하게 나타났다. 이는 향후 GOCI 자료의 응용적 측면에서 매우 고무적이다.

• 대한원격탐사학회지
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• 제16권1호
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• pp.55-64
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• 2000

열대 강우 관측 위성(TRMM: Tropical Rainfall Measuring Mission)에서 관측된 자료가 한반도에서 발생한 호우 특성을 얼마나 잘 나타내 주는지를 알아보기 위하여 1998년과 1999년 여름철 호우 사례에 대해 분석하였다. 본 연구에서는 먼저 집중 호우를 동반한 비 구름대의 높이, 강수형태, 비 구름의 수직 분포 및 수평 단면도 상의 강우 분포를 조사하였으며, TRMM/PR 관측 강우 강도와 지상에서 관측된 자동기상관측 장비(AWS: Automatic Weather System)에서 관측된 값을 비교하였다. GMS-5 IR1과 AWS 지상관측 자료와 비교해 볼 때, TRMM/PR 관측자료는 기존의 자료로는 알 수 없는 강수 형태, 비 구름대의 높이 등에 대한 정보를 쉽게 제공해 주었으며 특히 비 구름대 내부에서의 강우강도 분포와 연직 발달 정도를 잘 묘사했다. 또한 위도-경도별 강우강도의 단면분포에서는 지형 효과에 의한 강우 특성도 알 수 있었다. TRMM/PR 관측 강우강도 값은 AWS 관측치에 비해 6 mm/hr 이하에서 더 많이 관측되었으며, 전체적으로는 지상 관측에 비해 적게 관측되는 경향을 보였다.

• The Korean Journal of Pain
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• 제34권4호
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• pp.501-508
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• 2021

Background: Yellow flags are psychosocial factors shown to be indicative of long-term chronicity and disability. The purpose of the study was to evaluate the psychometric properties of the Turkish Yellow Flag Questionnaire (YFQ) in patients with chronic musculoskeletal pain (CMP). Methods: The cross-cultural adaptation was conducted with translation and back-translation of the original version. Reliability (internal consistency and test-retest) was examined for 231 patients with CMP. Construct validity was assessed by correlating the YFQ with the Hospital Anxiety and Depression Scale (HADS), Orebro Musculoskeletal Pain Questionnaire (OMPQ), and Tampa Kinesiophobia Scale (TKS). Factorial validity was examined with both exploratory and confirmatory factorial analysis. Results: The YFQ showed excellent test/retest reliability with an Intraclass correlation coefficient of 0.82. The internal consistency was moderate (Cronbach's alpha of 0.797). As a result of the exploratory factor analysis, there were 7 domains compatible with the original version. As a result of confirmatory factor analysis, the seven-factor structure of YFQ was confirmed. There was a statistically significant correlation between YFQ-total score and OMPQ (r = 0.57, P < 0.001), HADS-anxiety (r = 0.32, P < 0.001), HADS-depression (r = 0.44, P < 0.001), and TKS (r = 0.37, P < 0.001). Conclusions: This study's results provide considerable evidence that the Turkish version of the YFQ has appropriate psychometric properties, including test-retest reliability, internal consistency, construct validity and factorial validity. It can be used for evaluating psychosocial impact in patients with CMP.