• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.189-195
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• 2012

In plants, the fifth step of the plastidial 2-Cmethyl-D-erythritol 4-phosphate (MEP) pathway is catalyzed by 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECP; EC: 4. 6. 1. 12), an enzyme proposed to play a key role in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 823 bp Brassica rapa MECP (Brmecp) cDNA encoding a deduced polypeptide of 230 amino acid residues. Transcription levels of Brmecp were two-fold higher in petal compared to leaves. In addition, Brmecp expression in cabbage seedlings treated with ABA, $H_2O_2$ and drought was higher than control seedlings. These results were consistent with changes in chlorophyll contents in transgenic Arabidopsis. Thus, the Brmecp may contribute to the production of primary (chlorophylls and carotenoids) isoprenoid end-products in chloroplasts.

• 한국초지조사료학회지
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• 제14권4호
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• pp.316-323
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• 1994

In order to reduce the problems from the presence of rye crop residues in maize establishment and yield, and also to improve the growth, productivity and nutritive value of rye(Seca1e cereal L.). an experiment was canied out to determine the variety effect of rye on the forage production system and the eft'ect of seeding and harvesting dates on the production and quality of rye. 'This experiment was conducted at the forage testing field of S.N.U., Suweon, from September 1986 to May 1989. Heading date of an early maturing rye variety, Wintermore. was earlier 10 days than that of a late maturing rye variety, Kodiak. A 15-days delay in the seeding dates of early and late maturing varieties of rye tended to delay the heading dates of the rye varieties for 3 and 4 days, respectively. Dry matter and in vitro digestible dry matter yields were markedly increased with earlier seeding date. Before 20 April, the DM and IVDDM yeilds of an early maturing rye variety, Winterrnore. were higher than those of a late maturing rye variety, Kodiak. However, no such a trend was found between the early and late maturing varieties of rye after 27 April. Less than 35% of ADF was recorded until the harvesting dates of 13 and 27 p r i l for early and late maturing rye varieties, respectively, but less than 46% of NDF was maintained until the harvesting drtte of 13 April for botg varieties. Based on the results obtained from this experiment. it may be concluded that the most desirable forage production from corn-rye double cropping system is to advance the seeding time of rye toward the first 10 days of September as well as harvesting time toward the first heading stage with an early maturing rye variety.

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.947-953
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• 2011

A fungal strain, Aspergillus terreus strain GA2, isolated from an agricultural field cultivating sweet sorghum, produced feruloyl esterase using maize bran. In order to obtain maximum yields of feruloyl esterase, the solid state fermentation (SSF) conditions for enzyme production were standardized. Effective feruloyl esterase production was observed with maize bran as substrate followed by wheat bran, coconut husk, and rice husk among the tested agro-waste crop residues. Optimum particle size of 0.71-0.3 mm and moisture content of 80% favored enzyme production. Moreover, optimum feruloyl esterase production was observed at pH 6.0 and a temperature of $30^{\circ}C$. Supplementation of potato starch (0.6%) as the carbon source and casein (1%) as the nitrogen source favored enzyme production. Furthermore, the culture produced the enzyme after 7 days of incubation when the C:N ratio was 5. Optimization of the SSF conditions revealed that maximum enzyme activity (1,162 U/gds) was observed after 7 days in a production medium of 80% moisture content and pH 6.0 containing 16 g maize bran [25% (w/v)] of particle size of 0.71-0.3 mm, 0.6% potato starch, 3.0% casein, and 64 ml of formulated basal salt solution. Overall, the enzyme production was enhanced by 3.2-fold as compared with un-optimized conditions.

• 농약과학회지
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• 제9권4호
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• pp.359-364
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• 2005

박과채소류 중 살포농약의 과실 중 잔류소실 양상을 규명하여 잔류성 평가 시 작물계통 및 재배양식별로 group화 함으로서 잔류성적을 상호적용 할 수 있는 가능성을 평가하였다. 작물의 종류별로 농약살포직후에 과실에 부착되는 양은 차이가 있으나, 농약살포 후 경과일수별 잔류농약의 분해.소실양상은 유사함을 알 수 있었다. 박과채소류 중 작물생육특성이 유사한 품종(계통)간에는 농약의 작물잔류시험성적을 상호적용 하여 잔류허용기준 설정에 활용 할 수 있을 것으로 사료되었다.

• 한국토양비료학회지
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• 제44권3호
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• pp.473-478
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• 2011

A wide diversity of liquid fertilizers and composts produced from the livestock manure in Korea is commonly applied to agricultural lands as an alternative of chemical fertilizers. However, their effects on the crop production and environmental impacts are still vague. The current study was conducted to understand the effects of the pig manure-based liquid fertilizer on the growth of Zea mays L. and soil properties. Four different liquid fertilizers were treated to each cultivated upland plot located in Gyeong-gi province, Korea while no fertilizer (control A) and a chemical fertilizer (control B) were treated to separate plots for comparison. The liquid fertilizer treatment did not make a significant difference in the fresh weight of Zea mays L. compared to the controls. This is probably due to the nutrient residues carried over from the last year fertilization. Electric conductivity (EC) and organic matter contents in soils were increased right after the liquid fertilizer treatments compared to the controls. However, soil pH was maintained as the same as the level of control A. A long-term effect of the continuous treatment of the manure based liquid fertilizer will be carried out in the successive study.

• 한국육종학회지
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• 제43권5호
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• pp.479-489
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• 2011

Gluten is the main functional component of wheat, and is the main source of the viscoelastic properties in a dough. One of the gluten group is glutenin, which is composed of high molecular weight (HMW) and low molecular weight (LMW) subunits. The HMW glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the grain. They are encoded by the Glu-1 loci located on the long arms of homeologous group one chromosomes, with each locus comprising two genes encoding x- and y-type subunits. The presence of certain HMW subunits is positively correlated with good bread-making quality. The highly conserved N- and C- terminal contaning cystein residues which form interand intra-chain disulphide bonds. This inter chain disulphide bonds stabilize the glutenin polymers. In contrast, the repetitive domains that comprise the central part of the HMW-GS are responsible for the elastic properties due to extensive arrays of interchain hydrogen bonds. In this review, we discuss HMW-GS, HMW-GS structure and gluten elasticity, relationship between HMW-GS and bread wheat quality and genetic engineering of the HMW-GS.

• 한국환경농학회지
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• 제40권3호
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• pp.161-168
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• 2021

BACKGROUND: Biochar has ability to reduce N loss, increase crop yield, and sequestrate carbon in the soil However, there is still limited study concerning the interactive effects of various biochars on NH₃ loss and plant growth. This study, therefore, was conducted to investigate the NH₄⁺ adsorption characteristics of biochar derived from rice and maize residues. METHODS AND RESULTS: By-products were pyrolyzed under oxygen-limited conditions at 300-700℃ for 1 hour and used for experiment of NH₄⁺ adsorption in aqueous solution. The adsorption characteristics of biochar were studied using Langmuir isotherm. Biochar yield and hydrogen content decreased with increasing pyrolysis temperatures, whereas pH, EC, and total carbon content increased. The biochar pyrolyzed at lower temperatures was more efficient at NH₄⁺ adsorption than those produced at higher temperatures. In addition, the R_L values, indicating equilibrium coefficient were between 0 and 1, confirming that the result was suitable for Langmuir isotherm. CONCLUSION: The maize stalk biochar pyrolyzed at 300℃ was the most efficient to adsorb NH₄⁺ from the aqueous solution. Furthermore, the adsorption results of this experiment were lower than those of other prior studies, which were ascribed to different experimental conditions such as ingredients, and pyrolysis conditions.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.401-406
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• 2020

본 연구에서는 경작지 포장 조사를 통한 인삼근의 endosulfan 흡수이행성(PUF)을 산출하고, 토양 유기물 함량과 endosulfan에 대한 인삼근의 PUF에 관한 상관성을 분석하였다. Endosulfan 흡수이행 시험포장의 총 endosulfan 잔류량은 0.013-0.136 mg kg-1이었다. Endosulfan에 대한 인삼근의 PUF는 0.243-1.708로 인삼근의 연령이 증가할수록 비대생장에 의한 PUF값 감소가 확인되었다. 또한, 토양 유기물 함량과 endosulfan에 대한 인삼근의 PUF값은 5% 유의수준에서 음의 상관관계가 있음을 확인하였다(R2=0.6102). 따라서, endosulfan 오염 우려지역에서의 인삼경작을 위해서는 가급적 높은 토양유기물 함량을 유지하는 것이 endosulfan의 흡수이행을 억제하는데 도움을 줄 수 있을 것이다.

• 한국환경농학회지
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• 제42권3호
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• pp.193-202
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• 2023

Residue monitoring of propiconazole (PCZ) in cabbage, shallot, and spinach was conducted under multi-trial greenhouse conditions. This study aimed to understand the fate of the applied fungicide in these vegetables. Furthermore, the associated health risk of PCZ in leafy vegetables was assessed through dietary risk assessment. Commercially available PCZ (22% suspension concentrate) was administered thrice according to the OECD fungicide application interval guideline. The plant samples were extracted using a slightly modified QuEChERS technique and analyzed using gas chromatography-tandem mass spectrometry. The average PCZ recovery was between 84.5% and 117.6%, with a <5% coefficient of variance. The dissipation of PCZ residue in cabbage, shallot, and spinach after 14 days was 96%, 90%, and 99%, respectively, with half-lives of <5 days. Meanwhile, dietary risk assessments of PCZ residues in the studied vegetables using the risk quotient (RQ) were significant < 100 (RQ < 100). Thus, the population groups considered in this study were not at substantial risk from consuming leafy vegetables sprayed with PCZ following critical, good agricultural practices.