• BMB Reports
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• v.40 no.5
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• pp.773-782
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• 2007

Different isolates of the soil bacterium Bacillus thuringiensis produce multiple crystal (Cry) proteins toxic to a variety of insects, nematodes and protozoans. These insecticidal Cry toxins are known to be active against specific insect orders, being harmless to mammals, birds, amphibians, and reptiles. Due to these characteristics, genes encoding several Cry toxins have been engineered in order to be expressed by a variety of crop plants to control insectpests. The cotton boll weevil, Anthonomus grandis, and the fall armyworm, Spodoptera frugiperda, are the major economically devastating pests of cotton crop in Brazil, causing severe losses, mainly due to their endophytic habit, which results in damages to the cotton boll and floral bud structures. A cry1Ia-type gene, designated cry1Ia12, was isolated and cloned from the Bt S811 strain. Nucleotide sequencing of the cry1Ia12 gene revealed an open reading frame of 2160 bp, encoding a protein of 719 amino acid residues in length, with a predicted molecular mass of 81 kDa. The amino acid sequence of Cry1Ia12 is 99% identical to the known Cry1Ia proteins and differs from them only in one or two amino acid residues positioned along the three domains involved in the insecticidal activity of the toxin. The recombinant Cry1Ia12 protein, corresponding to the cry1Ia12 gene expressed in Escherichia coli cells, showed moderate toxicity towards first instar larvae of both cotton boll weevil and fall armyworm. The highest concentration of the recombinant Cry1Ia12 tested to achieve the maximum toxicities against cotton boll weevil larvae and fall armyworm larvae were 230 ${\mu}g/mL$ and 5 ${\mu}g/mL$, respectively. The herein demonstrated insecticidal activity of the recombinant Cry1Ia12 toxin against cotton boll weevil and fall armyworm larvae opens promising perspectives for the genetic engineering of cotton crop resistant to both these devastating pests in Brazil.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.2
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• pp.296-300
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• 2005

Human-induced soil salinity is becoming a major threat to agriculture across the world. This salinisation occurs in both irrigated and rain-fed agricultural zones with the highest proportions in the arid and semi-arid environments. Livestock can play an important role in the management and rehabilitation of this land. There are a range of plants that grow in saline soils and these have been used as animal feed. In many situations, animal production has been poor as a result of low edible biomass production, low nutritive value, depressed appetite, or a reduction in efficiency of energy use. Feeding systems are proposed that maximise the feeding value of plants growing on saline land and integrate their use with other feed resources available within mixed livestock and crop farming systems. Salt-tolerant pastures, particularly the chenopod shrubs, have moderate digestible energy and high crude protein. For this reason they represent a good supplement for poor quality pastures and crop residues. The use of salt-tolerant pasture systems not only provides feed for livestock but also may act as a bio-drain to lower saline water tables and improve the soil for growth of alternative less salt tolerant plants. In the longer term there are opportunities to identify and select more appropriate plants and animals for saline agriculture.

• Korean Journal of Organic Agriculture
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• v.14 no.3
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• pp.305-313
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• 2006

This research carried out to investigate the effects of application of hairy vetch instead of duck-releasing in a large scale of duck-releasing cultivation. The results are as followed; Cultivation of hairy vetch, a green manure crop, for the winter season seemed to be a possible natural fertilizer to supply crop mineral nutrients. Application of hairy vetch residues improved physical properties of soil and was possible to assure better productivity of agricultural products. Introduction of hairy vetch into a large scale of duck-releasing cultivation reduced the pollution of agricultural water.

• Korean Journal of Medicinal Crop Science
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• v.14 no.5
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• pp.289-292
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• 2006

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of $H_2O$ and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and $8.49\;{\mu}g/mg$, respectively), their fermented wine (0.45 and $1.33\;{\mu}g/mg$, respectively) and wine residues (no detection).

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.86-86
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• 2017

Prolamins are the main seed storage proteins in cereals. Gluten proteins seem to be prolamins because their primary structure have the meaningful quantity of proline and glutamine amino acid residues. Gluten proteins are found in crops such as wheat (Triticum aestivum), barley (Hordeum vulgare), and rye (Secale cereale) which are major food crops in the Triticeae tribe. Glutenin and gliadin, hordein, and secalin are typical gluten proteins found in wheat, barley, and rye, respectively. Gluten affect grain quality so that many researches, such as isolation or characterization of their genes, have been carried out. To improve the quality of grains in the Triticeae tribe, it is necessary to understand the relationship within their gluten proteins and their evolutionary changes. The sequences of nucleotides and amino acids of gluten protein including glutenins, gliadins, hordeins, and secalins were retrieved from NCBI (https://www.ncbi.nlm.nih.gov/) and Uniprot (http://www.uniprot.org/). The sequence analysis and the phylogenetic analysis of gluten proteins were performed with various website tools. The results demonstrated that gluten proteins were grouped with their homology and were mostly corresponded with the previous reports. However, some genes were moved, duplicated, or disappeared as evolutionary process. The obtained data will encourage the breeding programs of wheat, barley, rye, and other crops in the Triticeae tribe.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.1
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• pp.101-106
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• 2006

Common thistle contains water-soluble substances that are phytotoxic to neighboring plant species. A series of aqueous extracts from leaves, stems, roots and flowers of common thistle (Cirsium pendulum Fisch.) were assayed against alfalfa (Medicago sativa) seedlings to determine their allelopathy, and the results showed highest inhibition in the extracts from flowers and leaves, and followed by stems, and roots. The extracts at 40 g dry tissue $L^{-1}(g\;L^{-1})$ applied on filter paper in a Petri-dish significantly inhibited root growth of test plant by 87%. Methanol extracts at 100 g $L^{-1}$ from leaves inhibited root growth of alfalfa and barnyardgrass (Echinochloa crus-galli) by 89 and 98%, respectively. Hexane and ethylacetate fractions of common thistle reduced alfalfa root growth more than did butanol and water fractions. Incorporation into soil with the leaf residues at $100g\;kg^{-1}$ inhibited shoot fresh weights of barnyardgrass and eclipta (Eclipta prostrate) by 88 and 58%, respectively, showing higher sensitivity in grass species. These results suggest that common thistle plants had allelopathic potential for eco-friendly vegetation management, and that especially their activities were differently exhibited depending on plant part.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.3
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• pp.188-192
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• 1990

Sixteen amino acids in the hydrolysates of fulvic acid fraction from 7 plant materials were determined. Analyzed amino acids were aspartic acid, glutamie acid, arginine, histidine, lysine, glycine, alanine, valine, leucine, isoleusine, phenylalanine, tyrosine, serine, threonine, proline, and methionine. Four crop residues, wild grass cuttings and forest tree litters were put under investigation. 1. The content of amino acids in fulvic acid fractions extracted after 90 days of compositing ranged from 0.15% to 0.53% by dry weight. The highest value was found in the fulvic acids of wild grass cuttings and the lowest in those of wheat straw, being equivalent to 1/5-1/31 of those found in humic acids. 2. The group of neutral amino acids shared the largest portion followed by acidic and basic amino acids. 3. Arginine was not detected in fulvic acid fractions from well decomposed residues. 4. Aromatic amino acids, phenylalanine and tyrosine, were virtually absent in fulvic acid fractions. 5. Glycine, glutamic acid and aspartic acid were the 3 major amino acids contained in fulvic acids of well decomposed residues. With glutamic acid and aspartic acid excluded, the decreasing order of concentration of amino acids was roughly in parallel with the increasing order of molecular weight.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.357-362
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• 2008

In order to survey the residual characteristics of pesticides and assess their safeties in the agricultural products from markets, the agricultural products were purchased from the wholesale and traditional markets in Cheongju and Jeonju and analyzed the pesticide residues in them. No pesticide residues were found in samples from Cheongju, whereas, in case of samples collected from Jeonju, 3 pesticides including chlorothalonil were detected from 5 samples such as eggplant from wholesale market and 3 pesticides including azoxystrobin were found in tomato and grape from traditional market. Pesticide residues were detected from 10.9% of the total samples and detection levels were less than their maximum residue levels. Their estimated daily intakes ranged from 0.00102 to 0.03616% of their acceptable daily intakes, representing residue levels of the pesticides detected were evaluated to be safe.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.401-405
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• 2012

This study was carried out to investigate characteristics of the pesticide residues in environment friendly cereal grains and root vegetables. The samples, 747 the environment friendly agricultural products were collected from supermarkets and retail stores in eight major cities in May and August 2012. Residues of 245 pesticides in the samples collected were analyzed by multiresidue methods using GC and HPLC. No pesticide residues were detected in all the samples, representing the environment friendly cereal grains and root vegetables were produced according to the guideline for prohibition of use of pesticides during crop cultivation.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.1
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• pp.16-22
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• 1997

Determination of N mineralization and nitrification potentials of selected Piedmont soils of North Carolina requires a better understanding of the influences of incorporated plant residues. The net N mineralization and nitrification were significantly influenced by the soil types and by plant species. The net N mineralization and nitrification of soils mixed with plant residues were consistently increased with successional incubation periods. The net mineralization and nitrification ranged from $9.77{\mu}g/g$ to $143.80{\mu}g/g$, and from $5.31{\mu}g/g$ to $145.66{\mu}g/g$ during the incubation periods, respectively. The net N mineralization was more influenced by NO3-N than by NH4-N. Overall, the greatest proportions of net N mineralization and nitrification occurred in Chewacla and Wehadkee and lowest in Enon and Mecklenburg. For the plant residues, the net N mineralization and nitrification were observed to be lowest in corn and highest in soybean. In a low-input agricultural systems, soybean may be planted as cover crop which may improve the nitrogen status of selected Piedmont soils of North Carolina.