• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.5
• /
• pp.726-731
• /
• 2003

The aim of this research was to identify candidate probiotic lactic bacteria among indigenous dadih lactic isolates. Dadih is an Indonesian traditional fermented milk of West Sumatra which is fermented naturally. Viability of the strain is critical in determining the capacity of lactic bacteria to induce immune stimulation as well as to colonize in the intestinal tract. Therefore, LAB are proposed to exert health promoting or probiotic effects in human, such as inhibition of pathogenic microflora, antimutagenic, and the reduction of cholesterol levels. This manuscript reports in vitro probiotic properties of indigenous dadih lactic bacteria, especially some important colonization factors in GI tract, such as lysozyme, acid and bile tolerance. Bile Salt Hydrolase (BSH) activity, spectrum of bacteriocin, and antimutagenic activity of bacterial cells were also assessed. Twenty dadih lactic isolates were screened further for their tolerance to low pH, at pH 2 and 3 as well as their bile tolerance. There were ten isolates classified as acid and bile acid tolerant, and further screened for lysozyme tolerance, BSH activity. The spectrum of bacteriocin activity of isolates was assayed using cell-free neutralized supernatants by agar spot test against variety of pathogens. Lc. lactis subsp. lactis IS-10285, IS-7386, IS-16183, IS-11857 and IS-29862, L. brevis IS-27560, IS-26958 and IS-23427, Leu.mesen.mesenteroides IS-27526, and L. casei IS-7257 each has good survival rate at low pH values and in the presence of lysozyme, and short lag time in the presence of 0.3 % oxgall. Lc. lactis subsp. lactis IS-11857 and IS-29862 each has high BHS activity, Lc. lactis subsp. lactis IS-10285 and IS-16183 each had a positive spectrum of bacteriocin activity against E. coli 3301 and Lysteria monocytogenes ATCC 19112, while L. brevis IS-26958 has high BHS activity as well as positive spectrum of bacteriocin against E. coli 3301, Lysteria monocytogenes ATCC 19112, and S. aureus IFO 3060. All of the ten dadih lactic strains performed in vitro acid and bile tolerance, indicating a possibility to reach the intestine alive, and display probiotic activities.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.35 no.7
• /
• pp.601-611
• /
• 2007

Most of mechanically jointed aircraft structures are always encountered the fretting damages on the contact surfaces between two jointed structural members or at the edges of fastener holes. The partial slip and contact stresses associated with fretting contact can lead to severe reduction in service lifetime of aircraft structures. Thus a critical need exists for predicting fretting crack initiation in mechanically jointed aircraft structures, which requires characterizing both the near-surface mechanics and intimate relationship with fretting parameters. In this point of view, a series of fretting fatigue specimen tests for 2024-T351 Al-alloy, have been conducted to validate a mechanics-based model for predicting fretting fatigue life. And included in this investigaion were elasto-plastic contact stress analyses using commercial FEA code to quantify the stress and strain fields in subsurface to evaluate the fretting fatigue crack initiation.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.7
• /
• pp.1108-1113
• /
• 2015

Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using a recombinant Escherichia coli strain overexpressing the E. coli MG1655 cadA gene, and various parameters were investigated for the whole-cell bioconversion of lysine to cadaverine. A high concentration of lysine resulted in the synthesis of pyridoxal-5'-phosphate (PLP) and it was found to be a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, consumption of 91% lysine and conversion of about 80% lysine to cadaverine were successfully achieved.

• Journal of the Korean Institute of Rural Architecture
• /
• v.14 no.3
• /
• pp.1-8
• /
• 2012

Due to difficulty of marriage with women in Korea, unmarried men in countryside tend to have an international marriage. A ratio of international marriage was 3.7% in 2004, but increased by over 10% in 2004 due to the change of population composition in countryside. Without social or national preparation on a multicultural family and an increase of elder who lives alone made problems such as changes of population composition in countryside, a high rate of divorce due to differences on culture and society. These problems are becoming critical problems in countryside, not only for a family. This Study has a purpose as below, Firstly, this study will examine living place of elder who live alone and multicultural families. Secondly, characteristics of using each space will be analyzed and then surveyed efficiency and satisfaction. The result of survey will be provided to space planning for improvement of living standard. The results of this study can be summarized as follows: The characteristics of the surveyed households, the elderly ratio of 70 to 80 units high, and when the elderly become less than 10 years, 51.6%, 77.4% of women were overwhelmingly high. The characteristics of multicultural families in the in the middle or early 2000s when immigration is concentrated and national origin in Vietnam was 66.7 %. Housing characteristics of households with elderly housing type, the ratio improved to 61.3% is Majority. Type strain and 29% in traditional houses, traditional housing type is 9.7%. And house of multicultural families improve housing type is 87.5% Majority. The main space of the housing for the elderly, most are usually satisfied. But generally low level of self-determined housing survey confers real. These results have lived a long time because of space adaptation and can be viewed as attachment. For the elderly passive use of space and simplify the Act and heating cooling at the expense of the use of housing space due to the reduction, but multicultural families is the space required for the growth of their children because there are more modifications required.

• Proceedings of the KWS Conference
• /
• 2002.10a
• /
• pp.661-667
• /
• 2002

Welding-induced buckling distortion is one of the most problematic concerns in both design and fabrication of welded thin-plate structures. This paper deals with experimental and numerical results of the welding-induced longitudinal and/or buckling distortion occurring in welding of 6mm-thick AH36 high strength steel plates. Effects of the heat input and the plate size on the distortion were experimentally evaluated for square plates. Bead-on-plate welding was performed with the submerged arc welding process along the middle line of plate specimens. Experimental results showed that the longitudinal distortion made a single curvature in the plate, and the distortion magnitude along the weld centerline was proportional to the heat input and the plate size. The experimental results were used to examine the validity of the numerical simulation procedure for welding-induced distortion where the longitudinal distortion mode and magnitude were numerically quantified. Three-dimensional, large deformation, welding simulations were performed for selected weld models. Numerical results of the distortion mode and magnitude were in a good agreement with experimental ones. Depending on the presence of halting the distortion growth during the cooling cycle of welding, the condition discriminating buckling distortion from longitudinal distortion was established. Eigenvalue analyses were performed to check the buckling instability of tested plates with different sizes subjected to different heat inputs. The perturbation load pattern for the analysis was extracted from longitudinal inherent strain distributions. Critical buckling curve from the eigenvalue analyses revealed that the buckling instability is manifested when plate size or heat input increases.

• Proceedings of the KWS Conference
• /
• 2002.10a
• /
• pp.583-588
• /
• 2002

The purpose of the study is to evaluate fracture toughness on the Laser and the electron beam welded joints of high tensile steels (HT500, HT550, HT650) by using 3-point bend CTOD and Charpy impact test. WM (weld metal) CTOD tests have been carried out using two kinds of CTOD specimen, the Laser beam welding (108mm length, and 24mm width, and 12mm thickness) and the electron beam welding (l71mm length, and 38mm width, and 19mm thickness). WM Charpy impact specimen is a standard V-notch type, and the temperature of the experiment is changed from -45 to 20 degree of centigrade. FE-analysis is also performed in order to investigate the effect of stress-strain fields on fracture characteristics. Results of the standard V-notch Charpy test are influenced by strength mis-match effect and the absorbed energy vE depends on crack path, and The transition temperature of Laser beam welded joints is more higher than that of electron beam welded joints. Results of the 3-point bend test give low critical CTOD and the crack path is in the weld metal of al specimens. These results indicate fracture toughness characteristics of the welded joints and transition temperature of HT500 are similar both a Laser beam welded joint and an electron beam welded joint. But the fracture toughness and the transition temperature of the electron beam welded joints of HT550 and HT650 are higher than those o the Laser beam welded joints.

• Journal of the Korean Geotechnical Society
• /
• v.19 no.1
• /
• pp.21-29
• /
• 2003

In this study, practical guidelines to check the possibility of some lateral movement of piled abutment were investigated. In these tests, both the depth of soft clay and the rate of embankment construction are chosen to examine the effect on lateral soil movements. The depth of soft clay layer varies from 5.2 m to 11.6 m, and the rate of embankment construction has two types : staged construction(1m/30days, 1m/15days) and instant construction. Various measuring instruments such as LVDTs, strain gauges, pressure cells, and pore pressure transducers are installed in designed positions in ordo. to clarify the soil - pile interaction and the short and long term behavior f3. piled bridge abutments adjacent to surcharge loads. The validity of the proposed guidelines by centrifuge test was compared with the observed performance by lateral movement index, F(Japan Highway Public Corporation) and modified I index(Korea Highway Corporation). Based on the results obtained, the critical values off and modified I, as a practical guidelines, are proposed as 0.03 and 2.0, respectively.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.26 no.12
• /
• pp.2581-2589
• /
• 2002

In this research, fretting tests were conducted in air to investigate the wear characteristics of fuel cladding materials with the fretting parameters such as normal load, slip amplitude, frequency and the number of cycles. A high frequency fretting wear tester was designed for this experiment by KAERI. After the experiments, the wear volume and the shape of wear contour were measured by the surface roughness tester. Tribologically transformed structures(TTS) were analysed by means of optical and scanning electron microscopes to identify the main wear mechanisms. The results of this study showed that the wear volume were increased with increasing slip amplitude, and the shape of wear contour was transformed V-type to W-type. Also, it was found that the critical slip amplitude was 168${\mu}{\textrm}{m}$. These phenomena mean that wear mechanism transformed partial slip to gross slip to accelerate wear volume. The wear depth increased with an increase of friction coefficient due to increase of normal load and frequency. The fretting wear mechanisms were believed that, after adhesion and surface plastic deformation occurred by relative sliding motion on the contact between two specimens, TTS creation was induced by surface strain hardening and wear debris were detached from the contact surface which were produced by the micro crack propagation and creation.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.16 no.5
• /
• pp.497-506
• /
• 2014

In this study, analysis results described in the companion paper was used to determine shapes of room-and-pillar underground structure. To select optimized shapes, structural stability, space applicability and vehicle applicability were considered. In the structural stability step, ratio between strength and stress of the pillar and the critical strain at the roof span were adopted. The space applicability was used to retain the sufficient space of underground structure as its purpose is for human activity. The vehicle applicability was used to consider a radius for rotation of construction equipments in the room-and-pillar underground structure. From the given procedure in this study, proper shapes of rock pillar and room can be selected to design supports at the pillar and roof.

• Nuclear Engineering and Technology
• /
• v.52 no.2
• /
• pp.337-343
• /
• 2020

Oxide dispersed strengthened (ODS) steel is an important candidate for Gen-IV reactors. Oxide embedded in Fe can help to trap irradiation defects and enhances the strength of steel. It was observed in this study that the size of oxide has a profound impact on the depinning mechanism. For smaller sizes, the oxide acts as a void; thus, letting the dislocation bypass without any shear. On the other hand, oxides larger than 2 nm generate new dislocation segments around themselves. The depinning is similar to that of Orowan mechanism and the strengthening effect is likely to be greater for larger oxides. It was found that higher shear deformation rates produce more fine-tuned stress-strain curve. Both molecular dynamics (MD) simulations and BKS (Bacon-Knocks-Scattergood) model display similar characteristics whereby establishing an inverse relation between the depinning stress and the obstacle distance. It was found that (110)_oxide || (111)_Fe (oriented oxide) also had similar characteristics as that of (100)_oxide || (111)_Fe but resulted in an increased depinning stress thereby providing greater resistance to dislocation bypass. Our simulation results concluded that critical depinning stress depends significantly on the size and orientation of the oxide.