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http://dx.doi.org/10.4014/jmb.1412.12052

Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration  

Kim, Hyun Joong (Department of Biological Engineering, College of Engineering, Konkuk University)
Kim, Yong Hyun (Department of Biological Engineering, College of Engineering, Konkuk University)
Shin, Ji-Hyun (Department of Biological and Chemical Engineering, Hongik University)
Bhatia, Shashi Kant (Department of Biological Engineering, College of Engineering, Konkuk University)
Sathiyanarayanan, Ganesan (Department of Biological Engineering, College of Engineering, Konkuk University)
Seo, Hyung-Min (Department of Biological Engineering, College of Engineering, Konkuk University)
Choi, Kwon Young (Department of Environmental Engineering, Ajou University)
Yang, Yung-Hun (Department of Biological Engineering, College of Engineering, Konkuk University)
Park, Kyungmoon (Department of Biological and Chemical Engineering, Hongik University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.7, 2015 , pp. 1108-1113 More about this Journal
Abstract
Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using a recombinant Escherichia coli strain overexpressing the E. coli MG1655 cadA gene, and various parameters were investigated for the whole-cell bioconversion of lysine to cadaverine. A high concentration of lysine resulted in the synthesis of pyridoxal-5'-phosphate (PLP) and it was found to be a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, consumption of 91% lysine and conversion of about 80% lysine to cadaverine were successfully achieved.
Keywords
Biotransformation; whole-cell biocatalyst; lysine decarboxylase; cadaverine; highconcentration reaction; Escherichia coli;
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