• 한국환경농학회지
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• 제37권2호
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• pp.79-86
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• 2018

BACKGROUND: Chinese cabbage biosynthesizes various phytochemicals including carotenoids and glucosinolates. Environmental stress has a major effect on the growth and yields of vegetables, and can significantly affect nutritionally important phytochemicals. Phytochemicals of plants are influenced by light, temperature, carbon dioxide, and growing conditions. The aim of this study was to investigate the effect of various light sources on carotenoid and glucosinolate contents in Chinese cabbage. METHODS AND RESULTS: [Experiment I] Set the control (field control, FC) on the ground. Using acrylic sunlight, experiments were set up transparency box (field transparency, FT), red box (field red, FR) and blue box (field blue, FB). [Experiment II] Set the control (chamber control, CC) in the greenhouse. Using plant growth chamber with artificial light, experiments were set up LED red (chamber red, CR), LED blue (chamber blue, CB), LED mixed red+blue (chamber red+blue, CRB) and fluorescent (chamber fluorescent, CF). After plant growth, Chinese cabbage was harvested at 110 days after sowing (DAS). The status of plants growth (leaf length, width, fresh weight etc.) was immediately investigated. Carotenoid and GSL contents were analyzed by HPLC. [Experiment I] Results documented that the ranges of total carotenoid contents were 25.39 ~ 58.80 mg/kg dry wt for lutein, 0.84~ 4.22 mg/kg dry wt for zeaxanthin, and 3.85~18.71 mg/kg dry wt for ${\beta}$-carotene. Lutein was the highest for the content and the largest for the variation as well. [Experiment II] Results documented that the ranges of total carotenoid contents were 24.66~137.96 for lutein, 2.51~20.65 for zeaxanthin, and 8.40~49.80 mg/kg dry wt for ${\beta}$-carotene. The total carotenoid contents of CR (156.62) and CB (115.90) were 1.6~2.3 times larger than the other treatments, and ${\beta}$-carotene content was about twice as high as that of the other treatments on the CR (38.74 mg/kg dry wt.). [Experiment I] Total GSL content was the highest in FT (19.76) that was higher 1.7 times than the lowest treatment ($11.39{\mu}mol/g\;dry\;wt$.). [Experiment II] The total content of GSL was highest in CRB (4.19) and lowest in CF ($2.88{\mu}mol/g\;dry\;wt$.). In the CRB, total GSL contents ($4.19{\mu}mol/g\;dry\;wt$.) was the highest. CONCLUSION: Total and individual carotenoid and GSL contents in Chinese cabbage show significant differences under different light sources. Red and blue lights contribute to significant carotenoids expression and antioxidant activity for nutrition and health benefits. These results concluded that the introduction of varying lights affected the synthesis of important nutrient compounds in Chinese cabbage. It is predicted that the application of good light source enhances the accumulation of functional compounds.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.544-546
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• 2000

Grb2 is an important adaptor protein in the mitogenic Ras signaling pathway of receptor tyrosine kinases, and contains one SH2 domain and two SH3 domains. The SH2 domain binds to specific phosphotyrosine motifs on receptors or adaptor proteins such as Shc. The SH2 domain antagonists may lead to blocking of the oncogenic Ras signals and to developing new antitumor agents. In the course of screening SH2 antagonists from natural sources, cslerotiorin (1) and isochromophilone IV (2) were isolated from a strain, Penicillium multicolor F1753, and their structures were established by NMR spectral data. The metabolites significantly inhibited the binding between the Grb2-SH2 domain and phosphopeptide derived from the Shc protein, with $IC_{50}$ values of $22{\;}\mu\textrm{M}{\;}and{\;}48{\;}\mu\textrm{M}$ for (1) and (2), respectively. The compounds are the first nonpeptidic inhibitors of the SH2 domain from a natural source.

• 한국생산제조학회지
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• 제20권6호
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• pp.830-836
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• 2011

Most of pitch/yaw reducers consist of several planetary geartrains. Planetary geartrains make gearboxes to be small and light, low noise and good efficiency. Most important thing in the planetary geartrain is load distribution on the gear tooth flank. In this study, the effect of output shaft bearings on the load distribution of gear tooth flank has been investigated. The commercial software was employed to compare the load distribution of two models depending on the bearing type. The spherical roller bearing(SRB) and the cylindrical roller bearing(CRB) were used as output shaft bearings in the $1^{st}$ model, and two taper roller bearings(TRB) were used in the $2^{nd}$ model. As a result, it was found that the $2^{nd}$ model. showed better performances on the load distribution of gear tooth flank, this results stated that the output shaft bearing system could be important consideration when designing reducers for wind turbine systems.

• 한국전자파학회논문지
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• 제30권2호
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• pp.169-172
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• 2019

FMCW 배열 안테나는 저비용 고해상도라는 장점 때문에 많은 분야에 널리 사용되고 있다. FMCW 배열을 이용하여 표적의 거리와 각도를 추정하기 위해서, 우선 deramped 수신신호로부터 거리-각도 행렬을 구성하고, 그 다음에 거리-각도 행렬에 2D-FFT와 같은 2차원 주파수 추정기법을 적용한다. 하지만, 이러한 주파수 추정기법은 bias 오차를 발생시키게 된다. 그 이유는 거리-각도 행렬의 두 개의 주파수가 서로 독립적이지 않기 때문이다. 따라서 FMCW 배열 안테나를 이용하여 표적의 거리-각도 동시추정을 위한 최대우도 기반 알고리즘을 제안하고, 거리와 각도 추정에 있어서 Cramer-Rao bound에 도달함을 보인다.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1245-1252
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• 2022

Induced pluripotent stem cells (iPSCs) can be generated from somatic cells using Oct4, Sox2, Klf4, and c-Myc (OSKM). Small molecules can enhance reprogramming. Licochalcone D (LCD), a flavonoid compound present mainly in the roots of Glycyrrhiza inflata, acts on known signaling pathways involved in transcriptional activity and signal transduction, including the PGC1-α and MAPK families. In this study, we demonstrated that LCD improved reprogramming efficiency. LCD-treated iPSCs (LCD-iPSCs) expressed pluripotency-related genes Oct4, Sox2, Nanog, and Prdm14. Moreover, LCD-iPSCs differentiated into all three germ layers in vitro and formed chimeras. The mesenchymal-to-epithelial transition (MET) is critical for somatic cell reprogramming. We found that the expression levels of mesenchymal genes (Snail2 and Twist) decreased and those of epithelial genes (DSP, Cldn3, Crb3, and Ocln) dramatically increased in OR-MEF (OG2^+/+/ROSA26^+/+) cells treated with LCD for 3 days, indicating that MET effectively occurred in LCD-treated OR-MEF cells. Thus, LCD enhanced the generation of iPSCs from somatic cells by promoting MET at the early stages of reprogramming.

• 한국ITS학회 논문지
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• 제12권4호
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• pp.33-43
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• 2013

최근 꾸준히 증가하고 있는 자동차 수요에 부응할 만한 도로의 건설 등 시설공급능력은 이미 한계점에 이르렀으며, 일반 시민들이 피부로 느끼는 가장 심각한 문제 중 하나로 도로교통 문제가 대두되고 있다. 이에 본 연구에서는 교통체계관리기법(Transportation System Management) 중 대중교통시설의 효율성을 높일 수 있는 방안인 중앙버스전용차로제를 대전광역시의 주요 간선도로인 계룡로(월평3~서대전4, 6.3km) 및 대덕대로(대덕대교4~계룡4, 2.6km)에 적용하여 미시적 시뮬레이션(VISSIM)을 통해 차로제별 효과분석을 실시하였다. 더불어 시뮬레이션 분석결과를 자가용 이용자에게 제시하여 대중교통수단인 버스로의 통행수단선택 전환에 영향을 주는 요인을 분석하였다. 시뮬레이션 분석결과에서는 가로변 버스전용차로제에서 중앙버스전용차로제로 전환하면서 일반차량들이 이용할 수 있는 차로가 감소하게 되어 일반차량의 통행에는 부정적인 영향이 나타났지만 버스의 통행에는 매우 긍정적인 영향이 나타났다. 이항로짓모형의 분석결과에서는 소득이 낮고 통행거리가 짧으며, 목적지의 주차가 용이하지 않을수록, 또한 중앙버스전용차로의 통행 경험이 있을수록 자가용 이용자가 버스로의 통행수단선택 전환에 긍정적인 영향을 미치는 것으로 나타났다.