• Title/Summary/Keyword: Crb2

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Movement range and behavior characteristics of Pagrus major by acoustic telemetry in Byeonsan Peninsular, Korea (음향 텔레메트리에 의한 변산반도에서의 참돔(Pagrus major)의 이동 범위 및 행동 특성)

  • HEO, Gyeom;HEO, Min-A;KANG, Kyoungmi;HWANG, Doo-Jin;SHIN, Hyeon-Ok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.57 no.1
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    • pp.34-44
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    • 2021
  • In order to collect basic information of response behavior of red seabream (Pagrus major) during pilling, works for constructing wind power station in Byeonsan Peninsular, Korea were investigated. Four cultured red seabream CRB1 to CRB4 [total length (TL): 27.1 ± 1.0 cm; body weight: 359 ± 30 g] were tagged with an acoustic tag and used in experiment. CRB1 and CRB2 to CBR4 were released on the sea surface at same time around the constructing site of the wind power plant on September 22, 2017 and July 18, 2018, respectively. The tracking of the CRB1 to CRB2 and CRB3 to CRB4 were conducted for two hours, approximately, using VR100 receiver including a directional hydrophone and VR2W receivers array consisted of 19 presence/absence receivers (VR2W receivers), respectively. The underwater noise level before (no pile driving works) and during pile driving works was measured 116.0-118.0 dB (re 1��Pa) and a maximum of 160 dB (re 1��Pa), respectively. CRB1 moved about 6.0 km with average swimming speed of 80.2 ± 20.5 cm/s for 2.1 hours without pile driving work. The average water depth of the sea bed on the route of CRB1 was 9.1 ± 0.4 m. CRB2 moved about 7.3 km with the average swimming speed of 96.8 ± 27.1 cm/s for 2.1 hours with pile driving work. The water depth of the sea bed on the route of CRB2 was 11.9 ± 0.6 m. At results of the Rayleigh's z-test two fishes CRB1 and CRB2 showed significant directionality in the movement (p < 0.01). Movement mean angles of CRB1 and CRB2 were 92.7 and 251.8°, respectively. CRB2, CRB3 and CRB4 exhibited the escaping behavioral response from the noise of source during the pile driving work. The swimming speed of the CRB2 exposed on the heavy underwater noise stimuli due to the pile driving work was 1.21 times faster than that of the CRB1 exposed on the ambient underwater noise in the study site.

Comparison of in Vivo Antibacterial Activities and Pharmacokinetics of New Carbapenem Derivatives, CRB 529 and CRB 550, in Mice and Rats (신규 Carbapenem 유도체 CRB 529 및 CRB 550의 생체내 항균효과와 약물동태의 비교)

  • 김준겸;민관기;이주몽;이홍우;김정우
    • YAKHAK HOEJI
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    • v.39 no.4
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    • pp.360-366
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    • 1995
  • 1-$\beta$-Methyl carbapenem-2-substituted pyrroudine derivatives. CRB 529 and CRB 550, were synthesized as investigational carbapenem derivatives. It has been reported that the in vitro antibacterial activities of the compounds against G(+) and G(-) bacteria were almost the same or more effective than those of imipenem (IPM) and meropenem (MEPM), and also showed better in vivo efficacy than MEPM and inlipeneni/cilastatin (IPM/CS) against representative G(-) organisms, P. aeruginosa and MRSA organisms, S. aureus. The antibacterial activities, pharmacokinetics and protective efficacy of IPM/CS and CRB 529 and CRB 550 wereconducted after subcutaneous or intravenous administration to mice and rats. The pharmacokinetic parameters of CRB 529 and CRB 550 in mice were as follows: the observed maximal serum concentrations (C$_{max}$) following I.V. administration were 87.5 and 101 $\mu\textrm{g}$/ml for CRB 529 and CRB 550, respectively, and 63.6 $\mu\textrm{g}$/ml for IPM/CS. The half-lives (t$_{1/2}$) were 14.0 and 12.0 n-dn for CRB 529 and CRB 550, respectively, and 14.8 min for IPM/CS. In rats, $C_{max}$ after I.V. administration were 74.0 and 91.8 $\mu\textrm{g}$/ml for CRB 529 and CRB 550, respectively, and 41.2 $\mu\textrm{g}$/ml for IPM/CS. The tissue levels of CRB 529 and CRB 550 and IPM/CS after I.V. administration at a dose of 20 mg/kg decreased by the following order: lung, heart, kindney, liver and spleen for CRB 529, lddney, liver. lung, heart and spleen for CRB 550 and kidney, lung, liver, heart, spleen and brain for IPM/CS. In systemic infection, CRB 529 and CRB 550 showed excellent efficacies against P. aeruginosa and S. aureus (MRSA) at a dose of 5 mg/kg. The PD$_{50s}$ were 0.80, 0.36 mg/kg for CRB 529 and CRB 550, respectively, and 3.22 mg/kg for IPM/CS against P. aeruginosa. The corresponding values against S. aureus (MRSA) were 76.0, 55.3 mg/kg for CRB 529 and CRB 550, respectively, and 146 mg/kg for IPM/CS. In local infection, the antibacterial activities of CRB 529 and CRB 550 were more effective than those of IPM/CS against intrarenal infection with E. coli and P. aeruginosa and also showed as effective as IPM/CS against respiratory tract infection with E. coli and P. aeruginosa at a dose of 5 mg/kg.

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Effect of Additive on the Chemical Composition of Tra Catfish (Pangasius hypophthalmus) By-product Silages and Their Nutritive Value for Pigs

  • Thuy, Nguyen Thi;Lindberg, Jan Erik;Ogle, Brian
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.6
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    • pp.762-771
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    • 2010
  • Two experiments were conducted to determine i) the fermentation characteristics of catfish by-product (C) ensiled with rice bran (RB) or sugarcane molasses (M) in different ratios, and ii) the digestibility of the silages in growing pigs. In the ensiling experiment, there were three ratios of C, ensiled with RB or M, of 8:2, 7:3 and 6:4 (wet basis for C and air-dry basis for RB and M). The six treatments were CRB8:2, CRB7:3, CRB6:4, CM8:2, CM7:3 and CM6:4, with 3 replications per treatment and seven sampling times. The pH of CRB7:3 and CRB6:4 decreased (p<0.05) from the first week and stayed stable until 8 weeks of ensiling, but the pH did not decrease in CRB8:2. The pH of CM8:2, CM7:3 and CM6:4 decreased rapidly from the first week until week 8. Dry matter (DM) and crude protein (CP) contents were slightly lower when catfish by-product was ensiled with RB than with M. Ammonia content in all treatments increased (p<0.05) during ensiling. Lactic acid content in silages with molasses increased from the first week, with the highest value at week 4. However, the lactic acid content in CRB8:2 did not increase with time and had the lowest value of all treatments. Acetic acid proportions of total volatile fatty acids were low at day 0, with the highest value at day 7, decreasing slowly until 8 weeks. The butyric acid proportion was highest at day 0 and decreased up to week 8. The proportion of propionic acid increased during ensiling. The digestibility experiment had a 4${\times}$4 Latin-square design and included 4 castrated crossbreed (Yorkshire${\times}$Landrace) male pigs fed four diets. The basal diet (BD) included rice bran, broken rice and maize meal, and the other three diets included fish meal (FMD) or catfish by-product ensiled with rice bran (CRBD) or sugarcane molasses (CMD). The silages chosen were those which gave the best results in the ensiling experiment for each additive. The coefficient of total tract apparent digestibility (CTTAD) of DM and organic matter (OM) was not significantly different among treatments. The CTTAD of CP was not different among the silage diets, but was lower in BD (p<0.01). The CTTAD of ether extract (EE) was higher in the catfish by-product silage diets than in FMD and BD. There was no significant difference in the digestibility of DM, OM, CP and EE between the fish meal and the by-product silages. In conclusion, catfish by-product can be successfully preserved by ensiling. Moreover, the total tract apparent digestibility of OM, CP and EE in ensiled catfish by-product was comparable with that of fish meal.

Effect of Chitosan on Shelf Life of Cooked Rice Contaminated Artificially with Bacillus sp (취반시 키토산 첨가가 Bacillus sp.에 오염된 쌀밥의 품질에 미치는 영향)

  • Park, La-Young;Lee, Shin-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.12
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    • pp.1589-1595
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    • 2007
  • This study was carried out to examine the effect of chitosan on the quality of cooked rice. Sensory quality of cooked rice added with 0.1% and 0.5% chitosan was investigated. Taste, flavor and overall acceptability of cooked rice with 0.1% chitosan solution was similar to cooked rice without chitosan. Quality characteristics of cooked rice artificially inoculated with Bacillus spore (CRB), such as number of viable cell, pH, hardness, cohesiveness and color were investigated and compared with CRB added with 0.1% chitosan (CRCB) during storage at $20^{\circ}C\;and\;30^{\circ}C$ for 3 days. The viable cell number of 0.1% chitosan contained in cooked rice inoculated with Bacillus (CRCB) was lower about $1{\sim}1.5$ log at $20^{\circ}C,\;0.5{\sim}1$ log at $30^{\circ}C$ than that of cooked rice inoculated with Bacillus (CRB). The pH of CRCB did not change during storage at $20^{\circ}C$ for 2 days, but decreased in CRCB and CRB during storage at $30^{\circ}C$. The difference of hardness was not noticeable between CRB and CRCB during storage. Cohesiveness and lightness (L value) of CRCB were higher than those of CRB during storage.

Microarchitectural Defense and Recovery Against Buffer Overflow Attacks (버퍼 오버플로우 공격에 대한 마이크로구조적 방어 및 복구 기법)

  • Choi, Lynn;Shin, Yong;Lee, Sang-Hoon
    • Journal of KIISE:Computer Systems and Theory
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    • v.33 no.3
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    • pp.178-192
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    • 2006
  • The buffer overflow attack is the single most dominant and lethal form of security exploits as evidenced by recent worm outbreaks such as Code Red and SQL Stammer. In this paper, we propose microarchitectural techniques that can detect and recover from such malicious code attacks. The idea is that the buffer overflow attacks usually exhibit abnormal behaviors in the system. This kind of unusual signs can be easily detected by checking the safety of memory references at runtime, avoiding the potential data or control corruptions made by such attacks. Both the hardware cost and the performance penalty of enforcing the safety guards are negligible. In addition, we propose a more aggressive technique called corruption recovery buffer (CRB), which can further increase the level of security. Combined with the safety guards, the CRB can be used to save suspicious writes made by an attack and can restore the original architecture state before the attack. By performing detailed execution-driven simulations on the programs selected from SPEC CPU2000 benchmark, we evaluate the effectiveness of the proposed microarchitectural techniques. Experimental data shows that enforcing a single safety guard can reduce the number of system failures substantially by protecting the stack against return address corruptions made by the attacks. Furthermore, a small 1KB CRB can nullify additional data corruptions made by stack smashing attacks with only less than 2% performance penalty.

Multi-Antenna Noncoherent ML Synchronization for UWB-IR Faded Channels

  • Baccarelli Enzo;Biagi Mauro;Pelizzoni Cristian;Cordeschi Nicola
    • Journal of Communications and Networks
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    • v.8 no.2
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    • pp.194-204
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    • 2006
  • This contribution focuses on the maximum likelihood (ML) noncoherent synchronization of multi-antenna transceivers working in faded environments and employing ultra-wideband impulse radio (UWB-IR) transmit technology. In particular, the Cramer-Rao bound (CRB) is derived for the general case of multiple input multiple output (MIMO) UWB-IR systems and used to compare the ultimate performance of three basic transmit schemes, thereinafter referred to as single input multiple output (SIMO), MIMO equal signaling (MIMO-ES), and MIMO orthogonal signaling (MIMO-OS) ones. Thus, the noncoherent ML synchronizer is developed for the better performing transmit scheme (i.e., the SIMO one) and its performance is evaluated under both signal acquisition and tracking operating conditions. The performance gain in the synchronization of UWB- IR signals arising by the utilization of the multi-antenna technology is also evaluated.

Effect of Feeding Cyanidin 3-glucoside (C3G) High Black Rice Bran on Nutrient Digestibility, Blood Measurements, Growth Performance and Pork Quality of Pigs

  • Kil, D.Y.;Ryu, S.N.;Piao, L.G.;Kong, C.S.;Han, S.J.;Kim, Y.Y
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.12
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    • pp.1790-1798
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    • 2006
  • Two experiments were conducted to investigate the effect of feeding cyanidin 3-glucoside (C3G) high black rice bran on nutrient digestibility, blood measurements, growth performance and pork quality of pigs. In Exp. I, a total of fifteen pigs (19.91${\pm}$1.80 kg, average initial body weight) were used in assay of nutrient digestibility and blood measurements. All pigs were allotted to 5 treatments with 3 replicates according to a completely randomized design (CRD) in an individual metabolic crate. Treatments included 1) CON: basal diet, 2) BRB-2: basal+brown rice bran 2%, 3) BRB-4: basal+brown rice bran 4%, 4) CRB-2: basal+C3G high black rice bran 2% and 5) CRB-4: basal+C3G high black rice bran 4%. The digestibility of dry matter (DM), crude protein (CP), crude fat (CF), crude ash (CA) and crude fiber (CF) was not affected by dietary treatments. Serum triglyceride (TG) and high density lipoprotein (HDL) cholesterol concentrations were not affected by addition of C3G high black rice bran. However, at the end of experiment, pigs fed rice bran showed decreased tendency in total cholesterol concentration. Especially pigs fed C3G high black rice bran showed significantly lower total cholesterol concentration compared to pigs fed brown rice bran (p<0.03). There was numerically lower total cholesterol concentration with increasing levels of black rice bran in the diet. In terms of serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT), there were no significant differences among treatments, even though pigs fed CRB-4 showed the lowest GOT concentration compared to other pigs. In Exp. II, sixteen finishing pigs (average initial body weight 89.96${\pm}$0.35 kg) were divided into 4 treatments to investigate the effect of feeding C3G high black rice bran on growth performance and pork quality. There were no significant differences in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) among the treatments. Pigs fed C3G high black rice bran showed numerical decrease in ADG and increase in FCR while not effecting feed intake. There was no significant difference in live weight, carcass weight, carcass rate, backfat thickness and carcass grade. However, pigs fed C3G high black rice bran tended to show lower backfat thickness than pigs fed basal diet. Pigs fed C3G high black rice bran showed a tendency of decreased TBA value than pigs fed basal diet, although there was no overall significant difference among treatments. In conclusion, nutrient digestibility, blood measurements, growth performance and pork quality were not significantly affected by feeding C3G high black rice bran to pigs. However, C3G high black rice bran might have an effect on lowering serum total cholesterol and decrease the TBA value in pork compared to control group and these effects might be due to high concentration of antioxidative compounds in C3G high black rice bran.

Synthesis of L-threo-2,3-Dihydroxyphenylserine (L-threo-DOPS) by Thermostable L-Threonine Aldolase Expressed in Corynebacterium glutamicum R (Corynebacterium glutamicum에서 발현된 L-Threonine Aldolase를 이용한 파킨슨병 치료제 L-threo-2,3-Dihydroxyphenylserine (L-threo-DOPS)의 합성)

  • Baik, Sang-Ho
    • Microbiology and Biotechnology Letters
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    • v.36 no.2
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    • pp.128-134
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    • 2008
  • In order to examine efficient L-threo-2,3-Dihydroxyphenylserine (L-threo-DOPS) synthesis process using whole cell biocatalyst, a thermostable L-threonine aldolase (L-TA), which cloned from Streptomyces coelicolor A3(2) and improved for stability, was expressed in a Corynebacterium glutamicum R strain. The constructed Corynebacterium expression vector, pCG-H44(1) successfully expressed L-TA in C. glutamicum R strain, but showed very low expression level. In order to improve the expression level, the expression vector named pCG-H44(2) was reconstructed by eliminating 1 nucleotide between SD sequence and start codon of L-TA. The pCG-H44(2) vector plasmid was able to overexpress L-TA approximately 3.2 times higher than pCG-H44(1) in C. glutamicum R strain (CGH-2). When the whole cell of CGH-2 was examined in a repeated batch system, L-threo-DOPS was successfully synthesized with a yield of 4.0 mg/ml and maintain synthesis rate constantly after 30 repeated batch reactions for 130 h.

Centromere Repeat DNA Originated from Brassica rapa is Detected in the Centromere Region of Raphanus sativus Chromosomes

  • Hwang, Yoon-Jung;Yu, Hee-Ju;Mun, Jeong-Hwan;Bok, Kwang;Park, Beom-Seok;Lim, Ki-Byung
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.751-756
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    • 2012
  • Fluorescence in situ hybridization (FISH) is a powerful tool for the detection of DNA sequences in the specific region of the chromosomes. As well as for the integrated physical mapping, FISH karyotype analysis has to be preceded. Karyotype of Raphanus sativus 'Wonkyo 10039' was analyzed by a dual-color FISH technique; using various repetitive DNA probes, including 5S rDNA, 45S rDNA, and centromere retrotransposon. The length of the somatic metaphase chromosome ranged from 1.35 to $2.06{\mu}m$ with a total length of $15.29{\mu}m$. The chromosome complements comprised of eight pairs of metacentrics and one pair of submetacentric. Bleached DAPI Band analysis revealed a heterochromatin region, covering 28.6% to 50.4% each chromosomes. 5S and 45S rDNA sequences were located on two and three pairs of chromosomes, respectively. The centromere retrotransposon of Brassica (CRB) is a major component in Brassica related species that has been maintained as a common centromere component. CRB signals were detected on the centromere and pericentromeric region of R. sativus 'Wonkyo 10039' and three basic Brassica species (B. rapa, B. nigra, and B. oleracea). These results will provide a valuable background for physical mapping and elucidation of the evolutionary relationship among the Brassica related species.

Axial compressive behavior of concrete-encased CFST stub columns with open composite stirrups

  • Ke, Xiaojun;Ding, Wen;Liao, Dingguo
    • Advances in concrete construction
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    • v.12 no.5
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    • pp.399-409
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    • 2021
  • The existing method to improve the coordination performance of the inner and outer parts of concrete-encased concrete-filled steel tube (CFST) composite columns by increasing the volume-stirrup ratio causes difficulties in construction due to over-dense stirrups. Thus, this paper proposes an open polygonal composite stirrup with high strength and high ductility CRB600H reinforced rebar, and seventeen specimens were constructed, and their axial compressive performance was tested. The main parameters considered were the volume-stirrup ratio, the steel tube size, the stirrup type and the stirrup strength. The test results indicated: For the specimens restrained by open octagonal composite stirrups, compared with the specimen of 0.5% volume-stirrup ratio, the compressive bearing capacity increased by 14.6%, 15.7% and 21.5% for volume-stirrup ratio of 0.73%, 1.07% and 1.61%, respectively. For the specimens restrained by open composite rectangle stirrups, compared with the specimen of 0.79% volume-stirrup ratio, the compressive bearing capacity increased by 7.5%, 6.1%, and -1.4% for volume-stirrup ratio of 1.12%, 1.58% and 2.24%, respectively. The restraint ability and the bearing capacity of the octagonal composite stirrup are better than other stirrup types. The specimens equipped with open polygonal composite stirrup not only had a higher ductility than those with the traditional closed-loop stirrup, but they also had a higher axial bearing capacity than those with an HPB300 strength grades stirrup. Therefore, the open composite stirrup can be used in practical engineering. A new calculation method was proposed based on the stress-strain models for confined concrete under different restrain conditions, and the predicted value was close to the experimental value.