Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Centromere Repeat DNA Originated from Brassica rapa is Detected in the Centromere Region of Raphanus sativus Chromosomes

  • Hwang, Yoon-Jung (Department of Horticultural Science, Kyungpook National University) ;
  • Yu, Hee-Ju (Department of Life Sciences, The Catholic University of Korea) ;
  • Mun, Jeong-Hwan (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Bok, Kwang (Department of Horticultural Science, Kyungpook National University) ;
  • Park, Beom-Seok (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lim, Ki-Byung (Department of Horticultural Science, Kyungpook National University)
  • Received : 2012.08.29
  • Accepted : 2012.09.19
  • Published : 2012.12.31
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Abstract

Fluorescence in situ hybridization (FISH) is a powerful tool for the detection of DNA sequences in the specific region of the chromosomes. As well as for the integrated physical mapping, FISH karyotype analysis has to be preceded. Karyotype of Raphanus sativus 'Wonkyo 10039' was analyzed by a dual-color FISH technique; using various repetitive DNA probes, including 5S rDNA, 45S rDNA, and centromere retrotransposon. The length of the somatic metaphase chromosome ranged from 1.35 to $2.06{\mu}m$ with a total length of $15.29{\mu}m$. The chromosome complements comprised of eight pairs of metacentrics and one pair of submetacentric. Bleached DAPI Band analysis revealed a heterochromatin region, covering 28.6% to 50.4% each chromosomes. 5S and 45S rDNA sequences were located on two and three pairs of chromosomes, respectively. The centromere retrotransposon of Brassica (CRB) is a major component in Brassica related species that has been maintained as a common centromere component. CRB signals were detected on the centromere and pericentromeric region of R. sativus 'Wonkyo 10039' and three basic Brassica species (B. rapa, B. nigra, and B. oleracea). These results will provide a valuable background for physical mapping and elucidation of the evolutionary relationship among the Brassica related species.

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References

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