• 한국전산구조공학회논문집
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• 제28권6호
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• pp.625-633
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• 2015

본 연구에서는 강풍 위험과 강풍 취약도의 합성곱을 통하여 강풍 위험도를 평가할 수 있는 확률적 체계를 수립하였으며, 수치적으로 개발한 모형으로 아파트 창호 시스템의 강풍 위험도를 평가하였다. 강풍 위험 모형은 1951년부터 2013년까지에 한반도에 영향을 준 태풍의 기후학적 자료를 몬테카를로 모사기법에 적용하여 개발되었다. 또한 몬테카를로 모사기법으로 창호 시스템의 저항성능과 풍하중의 확률 분포를 비교하여 강풍에 대한 4가지 피해단계의 구조적 파괴확률을 평가할 수 있는 취약도 모형이 개발되었다. 개발된 몬테카를로 모사기법으로 평가한 강풍 위험과 강풍 취약도는 각각 웨이블 분포와 로그정규분포로 곡선맞춤 되었으며, 합성곱을 통한 강풍 위험도 평가에 사용되었다. 본 연구에서 개발한 확률적 위험도 평가체계를 통하여 평가지역, 지표조도, 지형, 지붕 경사각, 건물 높이 등이 아파트 창호 시스템의 강풍 위험도에 미치는 영향성을 정량적으로 평가할 수 있었다. 향후 본 연구를 통하여 개발된 강풍 위험도 평가 모델은 평가지역의 존재하는 건축물에 대한 데이터베이스와 결합하여 손실추정 및 피해 저감대책 수립 등의 분야에서 활용이 가능할 것으로 판단된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.93-93
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• 2016

Large scale integrated circuits (LSIs) has been improved by the shrinkage of the circuit dimensions. The smaller chip sizes and increase in circuit density require the miniaturization of the line-width and space between metal interconnections. Therefore, an extreme precise control of the critical dimension and pattern profile is necessary to fabricate next generation nano-electronics devices. The pattern profile control of plasma etching with an accuracy of sub-nanometer must be achieved. To realize the etching process which achieves the problem, understanding of the etching mechanism and precise control of the process based on the real-time monitoring of internal plasma parameters such as etching species density, surface temperature of substrate, etc. are very important. For instance, it is known that the etched profiles of organic low dielectric (low-k) films are sensitive to the substrate temperature and density ratio of H and N atoms in the H2/N2 plasma [1]. In this study, we introduced a feedback control of actual substrate temperature and radical density ratio monitored in real time. And then the dependence of etch rates and profiles of organic films have been evaluated based on the substrate temperatures. In this study, organic low-k films were etched by a dual frequency capacitively coupled plasma employing the mixture of H2/N2 gases. A 100-MHz power was supplied to an upper electrode for plasma generation. The Si substrate was electrostatically chucked to a lower electrode biased by supplying a 2-MHz power. To investigate the effects of H and N radical on the etching profile of organic low-k films, absolute H and N atom densities were measured by vacuum ultraviolet absorption spectroscopy [2]. Moreover, using the optical fiber-type low-coherence interferometer [3], substrate temperature has been measured in real time during etching process. From the measurement results, the temperature raised rapidly just after plasma ignition and was gradually saturated. The temporal change of substrate temperature is a crucial issue to control of surface reactions of reactive species. Therefore, by the intervals of on-off of the plasma discharge, the substrate temperature was maintained within ${\pm}1.5^{\circ}C$ from the set value. As a result, the temperatures were kept within $3^{\circ}C$ during the etching process. Then, we etched organic films with line-and-space pattern using this system. The cross-sections of the organic films etched for 50 s with the substrate temperatures at $20^{\circ}C$ and $100^{\circ}C$ were observed by SEM. From the results, they were different in the sidewall profile. It suggests that the reactions on the sidewalls changed according to the substrate temperature. The precise substrate temperature control method with real-time temperature monitoring and intermittent plasma generation was suggested to contribute on realization of fine pattern etching.

• 한국지진공학회논문집
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• 제14권3호
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• pp.11-22
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• 2010

이 논문에서는 댐-호소계의 선형 및 비선형 지진응답 해석을 시간영역에서 엄밀히 수행할 수 있는 해석법을 제시하였다. 댐-호소계는 (1) 선형 또는 비선형으로 거동하는 댐체와 (2) 깊이가 균일하다고 가정한 호소 원역 및 (3) 댐체와 호소 원역 사이 불규칙한 형상의 근역의 세가지 부구조물로 구성된 연계 시스템으로 정식화되었다. 댐체는 선형 또는 비선형 유한 요소로 모델링되고, 호소 원역은 무한 영역으로의 에너지 방사를 엄밀하게 표현할 수 있도록 주파수영역에서 개발된 변위기반 전달경계를 시간영역에서의 포갬적분으로 변환하여 시간증분법과 결합이 용이하게 하였다. 호소 근역을 댐체와 호소 원역이라는 두 개의 부구조물 사이에 저장된 압축성 유체로 모델링하였다. 이 논문에서는 세 개의 부구조물로 구성되는 댐-호소계에 대해 비선형 시간영역 해석을 용이하게 하는 시간증분법을 유도하여 제시하였고 개발된 해석법을 다양한 형상의 댐-호소계의 지진응답 해석에 적용하여 그 정확성을 검증하였다. 이에 추가하여 제시한 기법을 콘크리트 댐의 비선형 지진응답 해석에 적용하여 손상 정도와 부위를 해석 결과로서 보여주었으며 동시에 제시한 기법이 내진성능 평가 등 실무에 바로 활용될 수 있음을 입증하였다.

• Clinical and Experimental Reproductive Medicine
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• 제28권3호
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• pp.183-190
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• 2001

Objective: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. Methods : Mouse ovaries were fixed and cut into serial sections (7 im thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell $II^{TM}$ system. The frozen sections were fixed in 70% ethanol and stained with hematoxylin and eosin, while the paraffin sections were stained with Multiple stain. Sections were dehydrated in graded alcohols followed by xylene and air-dried for 20 min prior to LCM. All reactions were performed in ribonuclease free solutions to prevent RNA degradation. After LCM, total RNA extraction from the captured oocytes was performed using the guanidinium isothiocyanate (GITC) solution, and subsequently evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) for glyceraldehyde-3-phosphate-dehydrogenase (GAPDH). Results: With the frozen sections, detection of the GAPDH mRNA expression in the number of captured 25 oocytes were not repeatable, but the expression was always detectable from 50 oocytes. With 25 oocytes, at least 27 PCR cycles were required, whereas with 50 oocytes, 21 cycles were enough to detect GA PDH expression. Amount of the primary cDNA required for RT-PCR was reduced down to at least 0.25 $\grave{i}$ l with 50 oocytes, thus the resting 19.75 il cDNA can be used for the testing other interested gene expression. Tissue-to-slide, tissue-to-tissue forces were very high in the paraffin sections, thus the greater number of cell procurement was required than the frozen sections. Conclusion: We have described a method for analyzing gene expression at the RNA level with the homogeneously microdissected cells from the small amount of tissues with complexity. We found that LCM coupled with RT-PCR could detect housekeeping gene expression in 50 oocytes captured. This technique can be easily applied for the study of gene expression with the small amount of tissues.

• 비파괴검사학회지
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• 제24권5호
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• pp.508-517
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• 2004

근접장의 특성과 근접장 마이크로파 현미경의 배경이론을 설명하였고 유전체 공진기 제작에 앞서 HFSS (high frequency structure simulator)를 이용한 모의 시뮬레이션을 기술하였다. 이것을 바탕으로 원통형 유전체 공진기를 제작하여 금속탐침과 결합한 근접장 마이크로파 현미경(near field scanning microwave microscope : NSMM)을 구성하였다. 제작한 유전체 공진기의 특성은 HFSS를 이용하여 모의 실험한 결과와 비교하였다 Tip의 기하학적 모양에 따른 공간분해능과 감도(sensitivity)를 연구하였고 contrast가 가장 좋은 hybrid tip을 개발하였다. 전도도가 서로 다른 금속시료에 따른 NSMM의 반사계수의 변화를 측정하였고 실험결과와 이론적 시료의 임피던스를 비교하였다. 마지막으로 유전체 공진기를 이용한 NSMM으로 공간 분해능이 $1{\mu}m$의 Cr과 NiFe 패턴의 이미지를 비접촉, 비파괴방법으로 얻었다.

• 한국전자파학회논문지
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• 제14권1호
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• pp.81-88
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• 2003

운전자의 편의를 위한 자동 주행 차량은 전방 차량의 방향을 인식하며 차량간의 거리를 유지하기 위한 시스템이 요구된다. 본 논문은 24 GHz대역에서 움직이는 차량의 방향 감지를 위한 마이크로스트립 배열 안테나를 제안하였다. 넓은 빔 폭을 가지는 8${\times}$2송신 배열 안테나와 좁은 빔 폭을 가지는 8${\times}$4의 수신 center배열 안테나, 8${\times}$8의 수신 left, right 배열 안테나를 각각 설계 제작하였다. 측정 결과 송신 안테나의 경우 50$^{\circ}$의 방위각 빔폭과 16.7 dBi의 이득을 가지며, 수신안테나의 경우 center, left, right 배열 안테나 각각 20$^{\circ}$, 13$^{\circ}$, 13$^{\circ}$의 방위각 빔 폭과 20 dBi 이상의 이득을 가진다. 또한 left, right 배열 안테나의 경우 $\pm$18$^{\circ}$의 지향각이 요구되는데 이를 잘 만족함을 알 수 있다. 측정된 방사 패턴은 시뮬레이션 결과와 잘 일치하였으며 따라서 설계된 안테나는 전방 차량이 scan 각도 이내에 들어오면 3개의 방향을 감지하는데 적합함을 알 수 있다.

• 생명과학회지
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• 제27권1호
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• pp.44-49
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• 2017

본 연구에서는 일반적으로 분리 및 분석에 가장 빈번히 사용되고 있는 C18 column과 UV 검출기가 장착된 액체크로마토그래피(HPLC)와 항산화 활성 측정에 사용되는 1, 1-diphenyl-2-picryl hydrazyl (DPPH) 라디칼 소거능 측정 방법을 결합한 HPLC-DPPH 동시 측정법의 최적화와 유용성 확인을 약용식물의 추출물을 대상으로 실시하였다. 최종적으로 적용된 HPLC-DPPH 동시 측정법의 유용성은 갈근, 건강, 유근피, 모과, 황기 등 5종 약용식물의 열수추출물과 대조군으로서 ascorbic acid의 라디칼 소거능을 측정하여 확인하였다. HPLC-DPPH 동시 측정에 앞서 추출액 중 고형분 함량을 refractometer를 사용하여 측정함으로써 추출 수율에 따른 활성 차이를 보정할 수 있도록 하였다. 갈근, 모과, 유근피 열수추출물의 라디칼 소거능이 대조군으로 사용된 ascorbic acid와 비교하여 7.77%, 4.71%, 4.19%로서 다른 열수추출물보다 상대적으로 높은 것으로 확인되었다. 이와 같은 측정법은 실제 활성 성분의 분리 및 분석에 있어서 불필요한 시간 및 시약의 낭비를 줄일 수 있는 유용한 수단이 될 수 있을 것으로 판단된다.

• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.31-44
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• 2008

The forced swim test (FST) is the most widely used model for assessing potential antidepressant activity. Although it has been shown that lateral septum is involved with the FST-related behavior, it is not clear whether antidepressant treatments could alter the FST-induced gene expression profile in the lateral septum. In the present study, the gene expression profiles in response to FST and reboxetine pretreatment were observed in the lateral septum of rats. Reboxetine is known as a most selective serotonin norepinephrine reuptake inhibitor. In addition, we compared the changes in gene expression profile between reboxetine response and nonresponse groups, which were determined by counting FST-related behavior. After FST, lateral septum from controls and reboxetine pretreated group were dissected and gene expression profiles were assessed using an Affymetrix microarray system containing 15,923 genes. Various genes with different functions were changed in reboxetine response group compared with reboxetine nonresponse group, In particular, pleiotrophin, orexin receptor 2, serotonin 2A receptor, neuropeptide Y5 receptor and thyroid hormone receptor $\beta$ were decreased in reboxetine response group, but Lim motif-containing protein kinase 1 (Limk1) and histone deacetylase 1 (HDAC1) were increased. Although further studies are required for direct roles of these genes in reboxetine response, the microarray may provide tools to find out potential target genes and signaling pathways in antidepressant response.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.100-101
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• 2004

Many researches in Korea have been performed to understand the pollution of stream waters by acid mine drainage. However, few studies have been conducted regarding the effect of particulate and colloidal fractions on the transport of trace metals. To estimate harmful effects of trace metals, it is important to evaluate the particulate and colloidal metals as well as dissolved metals, because particulate and colloidal fractions of trace metals play an important role in transport of trace metals and may adversely affect habitats and organisms in riverine system. Colloids are solids with effective diameters in size range from 0.001 $\mu$m to 1 $\mu$m. According to Jone et al. (1974), metals in surface water, like Al, Fe, and Mn, require filtration with pore-size membranes smaller than 0.45 $\mu$m to define dissolved concentrations. The main objective of this study is to understand the effects of particulate, colloidal, and truly dissolved fractions on the transport and fate of trace metals in acid mine drainage. This study was conducted for the Onjeong creek in the Uljin mine area. Sampling was carried out in 13 sites, spatially covering the area from mine dumps to the downstream Onjeong reservoir. To examine the metal partitioning between particulate, colloidal, and truly dissolved fraction, we used successive filtration techniques consisting of conventional method (using 0.45 $\mu$m membranes) and tangential-flow ultrafiltration (using 0.001 $\mu$mm membranes). Ultrafiltration may seperate much smaller particles from aqueous phase (Josephson, 1984; Hernandez and Stallard, 1988). The analysis of metals were performed by inductively coupled plasma - atomic emission spectrometer (ICP-AES: model Perkin Elmer OPTIMA3000XL). Anions such as SO$_4$, Cl and NO$_3$ were measured with ion chromatograph (IC: model Dionex 120). Sample analysis is still in progress. The preliminary data show that the studied creek is severely polluted by Al, Fe, Mn, Pb and Zn. Toward upstream sites with relatively lower pH, less than 50% of Al and Fe occur in the sorbed form on particles or colloids, whereas more than 80% of Al and Fe occur in the sorbed form in downstream sites or tributaries with relatively higher pH. Less than 30% of Zn is present in particle or colloidal forms in the whole range of creek. Truly dissolved fraction of trace metals is negatively correlated with pH. The Kd values for Al, Fe and Zn consistently increase with increasing pH and decrease with increasing particle concentration.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2006년도 학술발표회 논문집
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• pp.2-11
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• 2006

In the processes of hydrological cycle, when precipitation reaches the ground surface, water may become surface runoff or infiltrate into soil and then possibly further percolate into groundwater aquifer. A part of the water is returned to the atmosphere through evaporation and transpiration. Soil moisture dynamics driven climate fluctuations plays a key role in the simulation of water transfer among ground surface, unsaturated zone and aquifer. In this study, a one-layer canopy and a four-layer soil representation is used for a coupled soil-vegetation modeling scheme. A non-zero hydraulic diffusivity between the deepest soil layer modeled and groundwater table is used to couple the numerical equations of soil moisture and groundwater dynamics. Simulation of runoff generation is based on the mechanism of both infiltration excess overland flow and saturation overland flow nested in a numerical model of soil moisture dynamics. Thus, a comprehensive hydrological model integrating canopy, soil zone and aquifer has been developed to evaluate water resources in the plain region of Huaihe River basin in East China and simulate water transfer among precipitation, surface water, soil moisture and groundwater. The newly developed model is capable of calculating hydrological components of surface runoff, evapotranpiration from soil and aquifer, and groundwater recharge from precipitation and discharge into rivers. Regional parameterization is made by using two approaches. One is to determine most parameters representing specific physical values on the basis of characterization of soil properties in unsaturated zone and aquifer, and vegetations. The other is to calibrate the remaining few parameters on the basis of comparison between measured and simulated streamflow and groundwater tables. The integrated modeling system was successfully used in the Linhuanji catchment of Huaihe plain region. Study results demonstrate that (1) on the average 14.2% of precipitation becomes surface runoff and baseflow during a ten-year period from 1986 to 1995 and this figure fluctuates between only 3.0% in drought years of 1986, 1988, 1993 and 1994 to 24.0% in wet year of 1991; (2) groundwater directly deriving from precipitation recharge is about 15.0% t of the precipitation amount, and (3) about half of the groundwater recharge flows into rivers and loses through evaporation.