Clinical and Experimental Reproductive Medicine
- Volume 28 Issue 3
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- Pages.183-190
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- 2001
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Analysis of the Gene Expression by Laser Captured Microdissection (I): Minimum Conditions Required for the RNA Extraction from Oocytes and Amplification for RT-PCR
Laser Captured Microdissection을 이용한 유전자 발현에 대한 연구 (I): RT-PCR을 위한 난자의 RNA 추출 및 증폭을 위한 최소한도의 확립
- Park, Chang-Eun (Infertility Medical Center, CHA General Hospital) ;
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Ko, Jung-Jae
(Infertility Medical Center, CHA General Hospital) ;
- Cha, Kwang-Yul (Infertility Medical Center, CHA General Hospital) ;
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Lee, Kyung-Ah
(Infertility Medical Center, CHA General Hospital)
- Published : 2001.09.30
Abstract
Objective: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. Methods : Mouse ovaries were fixed and cut into serial sections (7 im thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell