• Journal of Embryo Transfer
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• v.10 no.1
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• pp.65-72
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• 1995

The purposes of this study were to produce cloned rabbit embryos and offsprings by nuclear transplantation(NT) using in vitro matured oocytes as nuclear recipient cytoplasm and to determine the effect of frozen nuclei donor embryos on the production efficiency of cloned embryos. The 8cell embryos were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline containing 10% fetal calf serum(FCS) at 40 hours after hGG injection. A portion of collected embryos were preserved at 4$^{\circ}C$ for 24 hours and a portion of them were frozen by vitrification method. The embryos used for donor nuclei were synchronized in the phase of Gi /S transition. The in vitro matured oocytes were used as recipient cytoplasm following removing the nucleus and the first polar body. The synchronized blastomeres from fresh, cooled or frozen embryos were injected into the enucleated oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 $\mu$sec at 1.0 W /cm in 0.28 M mannitol solution. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$incubator. Following in vitro culture of the NT embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The nuclear transplant embryos developed in vitro to 2- to 4-cell stage were transferred into the oviducts of synchronized recipient does. The results obtained were summarized as follows: 1. The fusion rates of the blastomeres from fresh, cooled and frozen embryos with the in vitro matured and enucleated oocytes were 100, 95.8 and 64, 3%, respectively. 2. Development in vitro to blastocyst was significantly(p<0.05) different between the cloned embryos with the blastomeres from fresh, cooled or frozen embryos as 39.0, 20. 9 and 15.7%, respectively. 3. The mean numbers of cell cycle per day during in vitro culture of cloned embryos blastomeres from fresh, cooled or frozen embryos was 1.31, 1.29 and 1.16, respectively. 4. A total of 77 nuclear transplant embryos were transferred into 6 recipient does, of which two offsprings were produced from a foster mother 31 days after embryo transfer.

• Research in Plant Disease
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• v.12 no.3
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• pp.240-248
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• 2006

Control of white rot which is one of the most serious apple diseases in Korea has mainly relied on periodical spray of protective fungicides. As the main inoculum source of the disease is pycnidiospores produced in the warts formed on affected stems of apple tree, it can be conceivable that inhibition of spore production might be an effective means for controlling the disease. Inhibitory efficacy of eight selected fungicides against sporulation of the fungus was assessed by counting the number of spores produced at detached warts treated with the fungicides of recommended dilution. They showed diverse effect on sporulation. Carbendazim and azoxystrobin suppressed sporulation almost completely, the former irreversively. Thiram and folpet promoted sporulation as producing much more number of spores than untreated control. Others showed almost no effect on sporulation. Effects of suppression and promotion in the sporulation shown by the fungicides on the control of white rot were examined by incidences of disease and infection at the plots adopted the spray programs of which the fungicide at late May was substituted by carbendazim, azoxystrobin, folpet and thiram, respectively. Disease incidence and infection frequency at the plots sprayed former two chemicals which suppressed sporulation were much lower than those of the plots adopted latter two chemicals and untreated plot at which the fungicide spray was skipped at that time. These facts were reconfirmed in the experiments conducted with carbendazim and thiram, in which 100 fruits were bagged just prior to each spray from late May to late July for elucidating the effect of the two fungicides on the action of subsequent ones. Disease incidence and infection frequency on the fruit bagged just prior to each spray were gradually increased as the seasons going on. The increase rate at the carbendazim plot was much lower than that of thiram. Especially, the fruit infected till late July at the carbendazim plot were almost completely cured by the three fungicides, iminoctadine-triacetate, tebuconazole and samzinwang, a combined formular of iminoctadine-triacetate and difenoconazole, sprayed at late July and hence. In thiram plot, infected fruit were also cured by the 3 fungicides but not remarkable. From these results, it can be concluded that control efficiency of white rot can be greatly enhanced by selecting the fungicide capable of suppress the sporulation of white rot fungus at the season when the mass dispersal of spores is not initiated.

• Journal of Korean Society of Transportation
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• v.29 no.2
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• pp.111-121
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• 2011

This paper discusses a method for optimizing the semi-actuated traffic signal control system by adjusting the initial interval according to the number of vehicles waiting for the green light in the actuated phase. We also present a Left-Turn actuated traffic signal control strategy that examines the vehicular noise in the detection area and determines the phase extension and the gap-out. In order to detect the vehicles in real-time, an image detector's Video Image Tracking technology was adopted. A 'Zone in Zone'method was implemented, and the image detection area is segmented into three zones: 1) Zone1 for verifying a vehicles obligatory presence, 2) Zone2 for counting the standby vehicles, and 3) Zone3 for examining the number of vehicles that have passed. The on-site assessment of the Left Turn Actuated Control is carried out using CORSIM, and the results show that the Control Delay decreased by 23.10%, 15.06%, and 4.34% compared to the delays resulted from pre-timed control, semi-actuated control-1 and semi-actuated control-2 traffic signal control systems respectively. The Queue Time also decreased by 36.24%, 20.10% and the Total Time by 14.36%, 7.02% for the same scenario. Which clearly demonstrates the operational efficiency. A sensitivity analysis reveals that the improvement from the propose traffic control strategy tends to increase as the through traffic volume reaches a saturated condition and the left-turn traffic volume decreases.

• Journal of Conservation Science
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• v.24
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• pp.75-83
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• 2008

In order to assess antifungal activity of a wooden storage box, which was made of Paulownia tomentosa and used for keeping ancient documents, antifungal activity of volatile organic compounds emitted from the box was investigated along with qualitative analysis on major substances of the compounds. After collecting floating microorganisms inside air tester, the fungal activity was assessed by counting the number of colonies growing on TSA media. Compared to the control which collected 85 colonies from outdoor, 72 colonies were observed showing reduction rate of 14.82%. Through GC/MS and TDS system analysis, limonene was detected from the volatile organic compounds as characteristic features. When the fungal activity was assessed through fumigation by adding natural biocide BI and BII containing eugenol and anethole as major substances, both biocides showed a strong fungal activity with respectively 92.6%(inside the box) and 99.9%(outdoor) of reduction rate. Although these results didn't clarify antifungal activity of the volatile organic compounds emitted from the Paulownia-wood storage box and their functional components, it was at least confirmed that there is application possibility of natural biocide to use for preservation of ancient documents with increased efficiency in controlling pests of wooden storage boxes.

• Nuclear Engineering and Technology
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• v.4 no.4
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• pp.340-344
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• 1972

Isomeric ratios were measured for the capture of thermal neutron by $^{79}$ Br, $^{80}$ Se, $^{103}$ Rh, $^{115}$ In and $^{133}$ Cs as well as those of epi-cadmium neutron by $^{79}$ Br, $^{80}$ Se and $^{l33}$Cs. The measurements were performed by analysing decay curves obtained by ${\gamma}$-ray spectrometry after irradiation. The counting efficiency curve was determined by using the calibrated standard sources with overall uncertainties of about 1%. Isomeric ratios, given in $\sigma$ high spin/($\sigma$ high spin + $\sigma$ low spin), of $^{80, 80m}$Br, $^{81,81m}$Se, $^{014, 104m}$Rh, $^{116,116m}$In and $^{134, 134m}$Cs produced by thermal neutron activation were found to be 0.21$\pm$0.01, 0.14$\pm$0.02, 0.12$\pm$0.02, 0.69$\pm$0.07 and 0.058$\pm$0.004, respectively, Those values of $^{80, 80m}$Br, $^{81,81m}$Se, and $^{134, 134m}$Cs Produced by epi-cadmium neutron were found to be 0.19$\pm$0.02, 0.29$\pm$0.02 and 0.074$\pm$0.011, respectively. The experimental values obtained were compared with the theoretical values deduced from the statistical model. There were the general agreements between the theory and the experiment.t.

• Journal of Radiation Protection and Research
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• v.41 no.4
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• pp.395-401
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• 2016

Background: Liquid scintillation counters (LSCs) are commonly used as an analytical method for detecting $^{222}Rn$ in groundwater because they involve a simple sample pretreatment and allow high throughout with an autosampler. The Quantulus 1220 is the best-selling LSC in Korea, but its production was stopped. Recently, a new type of LSC, the 300SL, was introduced. In this study, the 300SL was compared with the Quantulus 1220 in order to evaluate the ability of each apparatus to detect $^{222}Rn$ in groundwater. Materials and Methods: The Quantulus 1220 and 300SL were used to detect the presence of $^{222}Rn$. Radon gas was extracted from a groundwater sample using a water-immiscible cocktail in a LSC vial. The optimal analytical conditions for each LSC were determined using a $^{222}Rn$ calibration source prepared with a $^{226}Ra$ source. Results and Discussion: The optimal pulse shape analysis level for alpha and beta separation was 80 for the Quantulus 1220, and the corresponding pulse length index was 12 in the 300SL. The counting efficiency of the Quantulus 1220 for alpha emissions was similar to that of the 300SL, but the background count rate of the Quantulus 1220 was 10 times lower than that of the 300SL. The minimum detectable activity of the Quantulus 1220 was $0.08Bq{\cdot}L^{-1}$, while that of the 300SL was $0.20Bq{\cdot}L^{-1}$. The analytical results regarding $^{222}Rn$ in groundwater were less than 10% different between these LSCs. Conclusion: The 300SL is an LSC that is comparable to the Quantulus 1220 for detecting $^{222}Rn$ in groundwater. Both LSCs can be applied to determine the levels of $^{222}Rn$ in groundwater under the management of the Ministry of Environment.

• Journal of Life Science
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• v.31 no.8
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• pp.755-760
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• 2021

Swine diarrhea is a livestock disease that causes huge economic losses to pig farms. In general, diarrhea occurs because of the proliferation of pathogenic Escherichia coli (E. coli). The toxins produced by the proliferated E. coli cause edema in pigs. Although the proliferation of these coliforms can be prevented by using a vaccine, the vaccines containing chemically produced dead bacteria are not very effective, making it difficult to control the proliferation of E. coli. Therefore, there is a need to develop new, more effective vaccines. In this study, we prepared killed F4+ and F18ab+ E. coli, which induce diarrhea and edema in pigs, using the extracts of immune-induced silkworms containing antimicrobial peptides and examined their availability as a killed-bacteria vaccine. First, the antimicrobial activity analysis of the prepared immune-induced silkworm extract was conducted using the radial diffusion assay. The results showed high activity against both F4+ and F18ab+ E. coli. The production efficiency of E. coli dead cells was determined using the colony-counting method. The concentration of the E. coli dead cells was the highest (50 mg/ml) when treated at 4℃. In addition, the analysis of the prepared dead cells using a transmission electron microscope confirmed that E. coli leaked out of the cytoplasm and the cell membrane remained intact. Therefore, F4+ and F18ab+ E. coli produced using immune-induced silkworms extract are considered to be highly available as bacterial ghost vaccines that can help prevent swine diarrhea and the resulting edema.

• KIPS Transactions on Computer and Communication Systems
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• v.12 no.8
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• pp.253-262
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• 2023

E-voting is a concept that includes actions such as kiosk voting at a designated place and internet voting at an unspecified place, and has emerged to alleviate the problem of consuming a lot of resources and costs when conducting offline voting. Using E-voting has many advantages over existing voting systems, such as increased efficiency in voting and ballot counting, reduced costs, increased voting rate, and reduced errors. However, centralized E-voting has not received attention in public elections and voting on corporate agendas because the results of voting cannot be trusted due to concerns about data forgery and modulation and hacking by others. In order to solve this problem, recently, by designing an E-voting system using blockchain, research has been actively conducted to supplement concepts lacking in existing E-voting, such as increasing the reliability of voting information and securing transparency. In this paper, we proposed an electronic voting system that introduced hybrid blockchain that uses public and private blockchains in convergence. A hybrid blockchain can solve the problem of slow transaction processing speed, expensive fee by using a private blockchain, and can supplement for the lack of transparency and data integrity of transactions through a public blockchain. In addition, the proposed system is implemented as BaaS to ensure the ease of type conversion and scalability of blockchain and to provide powerful computing power. BaaS is an abbreviation of Blockchain as a Service, which is one of the cloud computing technologies and means a service that provides a blockchain platform ans software through the internet. In this paper, in order to evaluate the feasibility, the proposed system and domestic and foreign electronic voting-related studies are compared and analyzed in terms of blockchain type, anonymity, verification process, smart contract, performance, and scalability.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.8 no.2
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• pp.53-61
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• 2022

This study aimed to increase the targeting ability against PSMA in cell therapy using metabolic glycoengineering and biorthogonal chemistry and to visualize cell trafficking using PET imaging. Cellular membranes of THP-1 cells were decorated with azide(-N₃) using Ac₄ManNAz by metabolic glycoengineering. Engineered THP-1 cells were conjugated with DBCO-bearing fluorophore (ADIBO-Cy5.5) for 1 h at different concentrations and analyzed by confocal fluorescence microscopy and flow cytometry. For PSAM ligand conjugation to THP-1 cells, Ac₄ManNAz treated THP-1 cells were incubated with DBCO-PSMA ligand (ADIBO-GUL) at a final concentration with 100 µM for 1 h. To evaluate the effect on cell recognition, PSMA ligand conjugated THP-1 cells(as effectors) were co-cultured with PSMA positive 22RV1 (as target cells) at 3 : 1 a effector-to-target cell (E/T) ratio. The interaction between THP-1 and 22RV1 was monitored by confocal fluorescence microscopy. For preparing the radiolabeled THP-1, the cells were treated at the activity of ~ 740 kBq of [⁸⁹Zr]Zr(oxinate)₄/5 × 10⁶ cells. Radiolabeled cells were analyzed for determination of cell-associated radioactivity by gamma counting and viability using MTS assay. In the cytotoxicity assay, THP-1 cells did not have any cytotoxicity even when the Ac₄ManNAz concentration was 100 µM. In confocal microscopy and flow cytometry, THP-1 cells were efficiently labeled ADIBO-Cy5.5 in a dose-dependent manner, and the dose of 100 µM was the optimal concentration for the following experiments. The clusters of PSMA ligand-conjugated THP-1 cells and 22RV1 cells were identified, indicating cell-cell recognition over the cell surface between two types of cells. Cell radiolabeling efficiency was 54.5 ± 17.8%. THP-1 labeled with 0.09 ± 0.03 Bq/cell showed no significant cytotoxicity compared to unlabeled THP-1 up to 7 days. We successfully demonstrated that Ac₄ManNAz treated cells were efficiently conjugated with ADIBO-GUL for preparing the PSMA-targeting cells, and [⁸⁹Zr]Zr(oxinate)₄ could be used to label cells without toxicity. It suggested that PSMA-ligand conjugated cell therapy could be improved cell targeting and be monitored by PET imaging.

• Journal of Appropriate Technology
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• v.5 no.2
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• pp.70-81
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• 2019

The garment industry is one of the most labor-intensive manufacturing industries, with its sewing process relying almost entirely on manual labor. Its costs highly depend on the efficiency of this production line and thus is crucial to determine the production rate in real-time for line balancing. However, current production tracking methods are costly and make it difficult for many Small and Medium-sized Enterprises (SMEs) to implement them. As a result, their reliance on manual counting of finished products is both time consuming and prone to error, leading to high manufacturing costs and inefficiencies. In this paper, a production tracking system that uses the sewing machines' energy consumption data to track and count the total number of sewing tasks completed through Convolutional Neural Network (CNN) classifiers is proposed. This system was tested on two target sewing tasks, with a resulting maximum classification accuracy of 98.6%; all sewing tasks were detected. In the developing countries, the garment sewing industry is a very important industry, but the use of a lot of capital is very limited, such as applying expensive high technology to solve the above problem. Applied with the appropriate technology, this system is expected to be of great help to the garment industry in developing countries.