• Archives of Pharmacal Research
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• 제23권5호
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• pp.459-460
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• 2000

Separation of the alkaloids from the aerial part of Corydalis ochotensis afforded a new spirobenzylisoquinoline alkaloid, 8-O-acetylcorysolidine along with two known spirobenzylisoquinoline alkaloids, isoochotensine and corysolidine.

• 대한한방부인과학회지
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• 제19권2호
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• pp.214-225
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• 2006

Purpose : This study was aimed to investigate the inhibitory effect of Corydalis Tuber on the proliferation of human uterine leiomyoma cell and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of suvival cells treated with indicated concentration of Corydalis Tuber and investigated cell viability by MTS assay. Furthermore, flow cytometric analyis were used to dissect between necrosis and apoptosis related with cell cycle and then we observed the differential gene expression by western blot analysis. Results : 1) The inhibitory effect on the proliferation of uterine leiomyoma cell treated with Corydalis Tuber was increased in a concentration and time proportional. 2) The result of flow cytometry analysis, subG1 phase arrest related cell apoptosis was not investigated in uterine leiomyoma cell treated Corydalis Tuber but showed G2/M phase prolongation. 3) The gene expression of p27, p21 related cell cycle was increased according to increasing concentration, but p53 was not exchanged. 4) The dephosphorylation of pRb gene were increased dependent on treatment concentration and pro-caspase 3, CDK4 were not exchanged. Conclusion : This study showed that Corydalis Tuber have the inhibitory effect on the proliferation of human uterine leiomyoma cell but the effect was thoughted no relationship with apoptosis. The inhibitory effect was suggested that dephosphorylation of pRb gene induced with increasing p21, p27 prolonged cell division in G2/M phase.

• Archives of Pharmacal Research
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• 제23권6호
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• pp.589-591
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• 2000

Six benzo[c]phenanthridine alkaloids, corynoline (1), acetylcorynoline (2), corynoloxine (3), luguine (4), 6-oxocorynoline (5), and 12-hydroxycorynoloxine (6) were isolated from the aerial parts of Corydalis incisa, and 6 was isolated for the first time from nature. The structure was elucidated by NMR techniques.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.817-819
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• 2002

In the course of screening Korean natural products for acetylcholinesterase (AChE) inhibitory activity, it was found that a methanolic extract of the aerial parts of Corydalis incisa (Papaveraceae) showed significant inhibitory effects on AChE. Corynoline isolated from this plant inhibited AChE activity in a dose-dependent manner, and the $IC_{50}$ value of corynoline was $30.6{\;}{\mu}M$. The AChE inhibitory activity of corynoline was reversible and noncompetitive.

• 약학회지
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• 제45권5호
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• pp.442-447
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• 2001

Aerial parts of Corydalis ochotensis Turcz. (Papaveraceae) has been used as a folk medicine in China for its antipyretic, analgesic and diuretic properties, which is widely distributed in Korea. Phytochemical study of the aerial parts of C. ochotensis led to the isolation of three isoquinoline alkaloids, (+)adlumidine, govadine and (-)severtzine, together with quercetin, rutin and tridecanoic acid. These compounds were established by conventional methods of analysis and identified by $^{1}$H,$^{13}$ C-NMR, and MS data.

• 한국균학회지
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• 제46권4호
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• pp.505-510
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• 2018

Peronospora is the largest genus of the order Peronosporales (Oomycota) and contains more than 550 accepted species, which causes downy mildew on many economically important crops. During a survey of downy mildew flora in Korea, a previously unreported species of Peronospora has been found on Corydalis ambigua. Based on molecular phylogenetic and morphological analyses, the causal agent was identified as Peronospora bulbocapni. This is the first report of Peronospora bulbocapni occurring on Corydalis ambigua in Korea.

• 생약학회지
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• 제30권1호
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• pp.54-58
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• 1999

A method using coloric and spectrophotometric detection have been developed for the identification of the tertiary or quaternary alkaloids contained in Corydalis tuber and its preparations. The principle is based on the formation or decomposition of complex compounds. The complex compound of the tertiary and quaternary alkaloids have been formed by adding tetrathiocyanatocobaltate [II] ion to the test soln. Diverse crude drugs were screened using this method and the results indicated that isoquinoline, aconitine-type alkaloids in crude drugs can be readily detected. The method is simple, convenient, reproducible and applicable to the verification of the crude drug Corydalis tuber and its preparations.

• 생약학회지
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• 제30권3호
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• pp.332-334
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• 1999

Corydalis Tuber has been used in traditional medicine for an analgesic, antispasmodic and gastric ulcers. For the quality control on this drug, isolation and quantitative determination of berberine and coptisine from Corydalis ternata Nakai (Papaveraceae) has been conducted by using HPLC method. Berberine and coptisine in quarternary alkaloidal fraction from the crude drug were separated on silicagel column using a $CHCl_3:MeOH\;(85:15)$ and $CHCl_3:MeOH:H_2O\;(70:30:4)$ as an eluent, and the average contents were about 0.93 and 0.36%.

• 한국의류산업학회지
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• 제19권5호
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• pp.620-625
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• 2017

In this study, the dyeing conditions(temperature, time, concentration) are changed from various conditions on wool fabrics by using corydalis tuber extract in order to develop new natural dyes. The purpose of this study is to improve the dyeability, color fastness, and functionality and to derive optimal dyeing conditions by comparing and analyzing the changes of K/S values and surface color by dyeing pH changes, mordant method, and mordant type. As a result of the experiment, the optimum dyeing condition of the wool fabrics is shown dyeing temperature:$80^{\circ}C$, dyeing time:90min, dyeing concentration:100%. The dyeability by pH variants of corydalis tuber extract indicates that K/S values is higher alkaline than acidic. The mordant method of corydalis tuber extract showed pre-mordant has high K/S values. In terms of color fastness, marked improvement has not been shown despite of mordant treatment on wool fabrics. In particular, color change of color fastness to washing, color fastness to light indicates the low fastness. In addition, the functionality such as antibacterial activities and deodorization can be given at dyeing with corydalis tuber extract thus it is expected to be applied to underwear or apparel products for the elderly and infirm and children with weak skin that required high functionality.

• 식물분류학회지
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• 제47권4호
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• pp.308-315
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• 2017

경상북도 봉화에서 현호색속의 신종인 봉화현호색(Corydalis bonghwaensis M. Kim & H. Jo)을 새로이 발견하여 기재하였다. 봉화현호색은 꽃이 흰색이고, 소화경에 털이 없으며, 괴경의 내부는 흰색이고, 주두돌기가 다각형이라는 점에서 남도현호색과 흰현호색과 유사하다. 그러나 봉화현호색은 꽃색이 연노랑에서 흰색으로 변하고, 내화피 정단은 약하게 돌출하며, 잎 열편은 가는 선형이고 2열 종자를 가진 방추형 삭과이다. 이에 반하여 남도현호색은 내화피 정단이 오목하게 함몰하고 잎 열편은 다양하며 2열 종자를 가진 방추형 삭과인 반면에, 흰현호색은 내화피는 오목하게 함몰하고 잎 열편은 타원형이며 1열 종자를 가진 선형 삭과이다. 또한 난장이현호색은 꽃색이 하늘빛 보라색이고 소엽은 타원형이며 꽃이 1-5개 달리고 아래 외화피가 반구형인 점에서 봉화현호색과 뚜렷이 구별된다.