• Korean Journal of Poultry Science
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• v.33 no.3
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• pp.195-202
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• 2006

Two experiments were conducted to investigate the effect of dietary selenium sources on performance and selenium retention in broiler chickens and laying hens. In experiment 1, the effects of dietary selenium sources and levels on the weight gain, feed intake, feed conversion, and selenium retention of meat in broiler chickens were investigated. for each growth phase, the basal diet was supplemented with 0 (control), 0.12 and 0.24 ppm Se from sodium selenite (SS) and 0.12, 0.24 and 0.60 ppm Se from selenium yeast(SY). Weight gain was significantly increased(P<0.05) in supplemental 0.24 and 0.60 ppm SY compared to the 0.24 ppm SS by diet during day 1 to 35, but feed intake and feed conversion were not affected by the source or the level of Se. Selenium concentrations of breast and leg muscle were significantly increased(P<0.05) in supplemental SS and SY compared to the control, and linearly increased(P<0.05) as dietary. Se level increased by SY, but there was no difference in supplemental 0.12 ppm SS compared to 0.24 ppm SS. In experiment 2, 12-week-experiment using Hy-Line laying hens(31 wk of age) was conducted to compare the effects of selenium sources and levels on egg production, egg weight, daily egg mass, feed intake, feed conversion, egg quality, and selenium retention of egg in laying hens. A corn-soybean meal basal diet was supplemented with 0 (control), 0.06 and 0.12 ppm Se from sodium selenite (SS) and 0.06, 0.12 and 0.30 ppm Se from selenium yeast(SY). Feed conversion was significantly improved(P<0.05) in supplemental 0.06 ppm SS compared to the control, but egg production, egg weight, daily egg mass, and feed intake were not affected by source and level of Se. Haugh unit was not affected by source or level of Se. Yolk color was significantly(P<0.05) higher in supplemental 0.3 ppm SY compared to the control and other supplement in week 12. Eggshell breaking strength was significantly(P<0.05) higher in supplemental 0.06 ppm SY(P<0.05). Thickness of eggshell was not affected by source or level of Se. Se concentrations of egg was significantly improved(P<0.05) in supplemental SS and SY compared to the control, and was significantly increased(P<0.05) as dietary Se level increased by SS and SY, especially SY more effective compared to the SS.

• Journal of the Korean Wood Science and Technology
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• v.42 no.4
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• pp.428-438
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• 2014

Functional effects of cauliflower mushroom (Sparassis latifolia) have been magnified by various media and internal and external research papers, recently. So, optimum condition of wood chip particle size and cultivation method of high ${\beta}$-glucan content for bulk cultivation generalization of cauliflower mushroom farms researched. As a result, T7 (1~2 mm 25%, 2~4 mm 50%, over 4 mm 25%) media as mixed media of certain ratio of particle size, showed excellent growth at $11.5{\pm}1.0$ cm / 44 days. Also, production of fruit body found higher than control and marketable pileus part took 85% ratio. The ${\beta}$-glucan content at media composition condition showed 1.4~2.4 times higher content in stipe part than pileus part. Also, PCF300 medium found 59.5% highest ${\beta}$-glucan content in stipe part. While ${\beta}$-glucan content showed 33.0% low content in pileus part. Therefore it needed additional study that ${\beta}$-glucan content improved in pileus part. In conclusion, production of high ${\beta}$-glucan content cauliflower mushroom was possible by T7 condition (wood chip particle size: 1~2 mm 25%, 2~4 mm 50% and over 4 mm 25%, composition: corn powder, flour and 300 ppm yeast).

• Korean Journal of Poultry Science
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• v.33 no.4
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• pp.255-262
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• 2006

Two experiments were conducted to investigate the effect of dietary organic selenium levels on performance and selenium retention in broiler chickens and laying hens. In experiment 1, the effects of dietary organic selenium levels on the weight gain, feed intake, feed conversion, and selenium retention of meat and liver in broiler chickens were investigated. For each growth phase, the basal diet was supplemented with 0 (control), 0.60, 1.20, 1.80 and 2.40 ppm Se from selenium yeast(SY). Weight gain, feed intake, and feed conversion were not affected by the selenium addition in diets. Breast muscle Se levels were linearly increased (P<0.05) as dietary Se level increased by SY. Selenium concentration of liver tissue was increased (P<0.05) in supplemental SY compared to the control, and was increased (P<0.05) in supplemental 1.20, 1.80 and 2.40 ppm SY compared to the 0.60 ppm SY. In Experiment 2, 12-week-experiment using Hy-Line laying hens (68 wk of age) was conducted to examine the effects of dietary organic selenium on egg Production, egg weight, daily egg mass, feed intake, feed conversion, egg quality, and selenium concentration of eggs. A corn-soybean meal basal diet was supplemented with 0 (control), 0.30, 0.60, 0.90 and 1.20 ppm Se from selenium yeast (SY). Egg Production was significantly improved(P<0.05) in supplemental 0.30 and 0.90 ppm SY compared to the control and 0.60 ppm SY during week 1 to 12, but daily egg mass, feed intake, and feed conversion showed no difference in supplemental SY and control. Haugh unit, yolk color and eggshell breaking strength showed no difference in supplemental SY and control. Eggshell thickess was significantly (P<0.05) higher in supplemental 0.60 and 1.20 ppm SY compared to the 0.90 ppm SY in week 9. Egg Se levels were linearly increased (P<0.05) as dietary Se level increased by SY.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.157-163
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• 2007

To examine the effects of dietary organic selenium and vitamin E supplementation on egg production, egg weight, daily egg mass, feed intake, feed conversion, egg quality, lipid-soluble antioxidative capacity (ACL) in egg yolk, and selenium retention of egg in laying hens, Hy-Line laying hens of 77 wk old were replaced in the individual cage for 12 week. A corn-soybean meal based diet was supplemented with 0 (control), vitamin E 100 IU/kg and the combination of 0.9 ppm Se from selenium yeast (SY) and vitamin E 50, 100 and 150 IU/kg. Egg production and daily egg mass were significantly increased (P<0.05) in supplemental vitamin E 100 IU, and 0.9 ppm SY + vitamin E 50 IU than control for the whole experimental period. However, feed intake and feed conversion were not affected by supplemental vitamin E and SY. Haugh unit was significantly (P<0.05) higher in supplemental vitamin E 100 IU and 0.9 ppm SY + vitamin E 50 IU than control, 0.9 ppm SY + vitamin E 100 and 0.9 ppm SY + 150 IU in week 5. Haugh unit of the eggs during storage decreased significantly(P<0.05) from day 1 to day 11. However, Haugh units of the eggs from 0.9 ppm SY + vitamin E 150 IU treated groups did not decreased significantly until day 5. Yolk color was significantly (P<0.05) higher in 0.9 ppm SY + vitamin E 50 and 0.9 ppm SY + 100 IU than other treatment groups in week 1. Selenium concentrations of egg were significantly increased (P<0.05) in 0.9 ppm SY + vitamin E than control and vitamin E 100 IU groups. Lipid-soluble antioxidative capacity (ACL) in egg yolk was significantly (P<0.05) higher in supplemental combination of 0.9 ppm SY + vitamin E 150 IU when compared to those of other treatment groups.

• Korean Journal of Organic Agriculture
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• v.19 no.2
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• pp.185-198
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• 2011

This study was carried out to select a proper forage crop, and to estimate the proper level of application of cattle manure and carrying capacity of organic livestock per unit area. Corns and forage sorghum hybrids were cultivated with different types of livestock manures and different amount of them to produce organic forage. For both corns and forage sorghum hybrids, no fertilizer plots had significantly (p<0.05) lower annual dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) yields than those of other plots, whereas the N-P-K (nitrogen-phosphorous-kalium) plots ranked the highest yields, followed by 150% cattle manure plots and 100% cattle manure plots. DM, CP and TDN yields of in cattle manure plots were significantly (p<0.05) higher than those of no fertilizer and P-K (phosphorous-kalium) plots. The yields of in cattle slurry plots tended to be a little higher than those of in composted cattle manure plots. Assuming that corn and forage sorghum hybrids produced from this trial were fed at 70% level to 450kg of Hanwoo heifer for 400g of average daily gain, the carrying capacity (head/year/ha) of livestock ranked the highest in 150% cattle slurry plots (mean 6.0 heads), followed by 100% cattle slurry plots (mean 5.3 heads), 150% composted cattle manure plots (mean 4.7 heads), 100% composted cattle manure plots (mean 4.4 heads), and no fertilizer plots (mean 2.8 heads) in corns (or the cultivation of corns). Meanwhile, in the case of forage sorghum hybrids, 150% cattle slurry plots (mean 6.4 heads) ranked the highest carrying capacity, followed by 150% composted cattle manure plots (mean 4.8 heads), 100% cattle slurry plots (mean 4.4 heads), 100% composted cattle manure plots (mean 4.1 heads), and no fertilizer plots (mean 2.8 heads). The results indicated that the application of livestock manure to cultivated soil could enhance not only DM and TDN yields, but also the carrying capacity of organic livestock as compared with the effect of chemical fertilizers. In conclusion, the production of organic forage with reutilized livestock manure will facilitate the reduction of environmental pollution and the production of environmentally friendly agricultural products by resource circulating system.

• Journal of Animal Environmental Science
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• v.17 no.sup
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• pp.7-20
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• 2011

This study was conducted to analyze the mixing ratio of raw feed materials for the methane mitigation and also to identify the minimum rearing density for improving the productivity of beef calves as eco-friendly fodder. Raw materials used in this study for the formulation of feed for methane reduction were crushed corn and alfalfa along with other 21 species. In addition, to investigate the appropriate rearing density, 12 Hanwoo calves with average weight of 150 kg was selected and experiment was conducted for four months. Methane gas emission (Bo) is about 3-4 times less in TMR 4 compared to TMR 1, 2 and 3. Feed price calculated for TMR 4 ration was also affordable. In addition, all TMRs showed a normal ruminal pH. Disappearance rate was observed to be lower in TMR 4 as compared to TMR 1, 2 and 3, but methane production decreased by 24 to 37%. The result showed improved total body weight, average daily gain and feed conversion ratio in rearing low-density ($18m^2/head$), and general treatment ($9m^2/head$) compared to overcrowding treatment ($6m^2/head$). In addition, blood components (total protein, glucose, AST, ALT and GGT factors) involved in health and disease treatments and health-related nutrition metabolism are lower in the low-density and general treatment compared to the high density treatment. Postural development (development of body size) i.e., weight, height and width significantly increased in the low and general density treatment compared to high density treatment. Especially excellent improvement was observed in low-density treatment than the general treatment. Moisture content, colonic bacteria and coccidium are higher in low and high density treatments than in the general treatment. The adequacy for beef rearing density is considered to be more desirable in an area more than $6m^2/head$. In conclusion, present study suggests that possibility of methane reduction through adjusting mixed feed ration. Also, rearing density is also an important factor in the growth and development of beef calves.

• Korean Journal of Poultry Science
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• v.18 no.1
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• pp.33-42
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• 1991

In order to study effects of the 9round charcoal and organic acid on the egg production, White Leghorn strain(Hyline) layer of 113 week-old were fed control diet during a week of previous feeding and subsquent experimental diets during 12 weeks of experimental feeding . The experimental diets were the basal (control) diet composed of mainly corn-soybean meal, diet (CPB) substituted 0.5％ of ground charcoal with the defatted rice bran of the control, diet (PWV) added 0.1 mM(based on the acetic acid) of wood vinegar in the CPD and diet(PFA) added 0.1 mM (based on the acetic acid) of fermented acetic acid in the CPD. During experimental feeding period, birds (ed control, CPD and PWV diets showed similar feed intake which was shown a decreasing tendency in birds fed PFA diet as the passage of experimental feeding period. Birds fed experimental diets containing ground charcoal were tended to increase egg weight (g/egg) , while which of birds fed CPD was higher(P＜0.05) than that fed control diet. Chicks fed the control, CPD and PWV diets produced 75.9, 75.0 and 75. 9％ of egg, respectively, which were significantly(P＜0.05) higher than those of birds fed PFA. Daily egg mass(g/bird) were reached to 48.6, 49.9 and 49.5g i,1 layer fed the control, CPD and PWV diets, respectively, showing an increasing tendency in layer fed CPD and PWV compared with those of birds fed control, which were significantly (P＜0.05) higher than those of birds fed PFA . Although the effect of experimental diets on the feed efficiency (egg/feed) was not found, which was shown an increasing tendency in layer fed CPD compared with those in control diet. Compared with the incidence of cracked or shelless eggs in birds fed control diet, which were lowered to 37％ in birds fed CPD diet and 70％ in PWV-fed birds and increased to two times in birds fed PFA . The contents of cholesterol in egg was not affected by experimental diets, while birds fed CPD diet increased significantly(P＜0.05) the daily biosynthesis of egg protein and showed a trend to increase the thickness of eggshell and eggshell contents per eggs compared with that of control diet-fed birds. The results indicated that the ground charcoal powder improved the performance of layer.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.607-614
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• 2005

To determine the optimum fermentation condition, the 50:50 feed mixture of both crushed corn and wet brewer’s grain were anaerobically fermented at various conditions. Three supplementation levels(0, 2%, and 5%) of molasses, five supplementation levels(0, 0.1%, 0.5%, 1.0%, and 2.0%) of yeast and five different incubation temperatures(4$^{\circ}C$, 10$^{\circ}C$, 30$^{\circ}C$, 40$^{\circ}C$ and 50$^{\circ}C$) were tested to determine the optimum fermentation conditions. During fermentation, alcohol concentration, live yeast cell number (LYCN) and pH values of the mixture were analyzed. Alcohol concentrations of the fermented feed mixtures were proportionally increased with increasing level of molasses supplementation. After 24hr fermentation, alcohol concentrations at 0.1%, 0.5%, 1.0% and 2.0% of yeast supplementation was 1.9%, 2.4%, 3.1% and 3.1%, respectively. These results indicate a proportional increase of alcohol concentration with the increasing supplementation of yeast (P<0.05) up to 1%, thereafter showing plateau at 2% supplementation. LYCN was relatively high with more than 0.5% yeast supplementation, whereas the value was significantly low(P<0.05) at 0.1% yeast supplementation. Alcohol production was significantly higher at 30$^{\circ}C$ and 40$^{\circ}C$ than other incubation temperatures (P<0.05), with the highest concentration at 30$^{\circ}C$. pH was rapidly decreased until 24 hours of incubation with steeper decrease at lower temperatures(P<0.05). Based on there results, optimum fermentation conditions for the alcoholic-fermented feed production could be achieved with 5% supplementation of molasses, 1% supplementation of yeast, and 24hr long incubation at 30$^{\circ}C$.

• Journal of Animal Science and Technology
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• v.52 no.5
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• pp.389-398
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• 2010

The objective of this study was to investigate the effects of three strains of Bacillus subtilis (B. subtilis) supplemented to diets on egg production, egg quality, egg yolk cholesterol levels, the profile of cecal microflora, and tibia characteristics in laying hens. One hundred sixty 76-week-old Hy-Line Brown layers were randomly divided into 4 groups with 4 replicates per group (10 birds per replicate). Birds in the control group were fed a corn-soybean meal based diet. The remaining three treated groups were fed the control diet containing either 0.05% B. subtilis Ch3 (T1), 0.05% B. subtilis Ch3 + B. subtilis W1 (T2) or 0.05% B. subtilis commercial product (T3) for 6 weeks, respectively. There were no differences in feed intake, egg weight, egg production and egg mass among the groups. The dietary supplementation of B. subtilis improved eggshell strength and Haugh units compared to those of control (P<0.05). The activities of GOT and GPT in serum were not also affected by the dietary treatments. The population of total microbes and lactic acid bacteria in cecum were significantly increased by the dietary B. subtilis (P<0.05), but not the coliforms. The cholesterol concentration in egg yolk and serum in the treated groups were significantly decreased compared to those of control (P<0.05). Also, The levels of phospholipids in serum were significantly decreased compared to those of control (P<0.05). The supplementation of three strains of B. subtilis to diets significantly increased the contents of tibia ash compared to that of control (P<0.05). Thus, this study showed significant improvements in egg quality, such as eggshell strength and Haugh unit, by dietary B. subtilis strains. The B. subtilis strains added to the diets modulated the profiles of cecal microflora, reflecting beneficial effects in laying hens.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.303-313
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• 2008

This research is aimed to investigate the cultivation method of oyster mushroom[Pleurotus ostreatus (Jacq. ex Fr.) Kummer] at the non-sterilized medium with $Ca(OH)_2$ treatment. Therefore, experiments were carried out to develop non-sterilization method of medium by addition of $Ca(OH)_2$ for omission of heat sterilization progress of medium. General components, minerals and amino acid in Jiri wild type No. 1 (Pleurotus ostreatus) and production cost were analyzed. For the purpose of omission of heat sterilization progress, treatment ratio of $Ca(OH)_2$ (purity 95%) was 5%(w/w) of dry medium. Initial pH of this medium was 11 and then the pH was changed by 9 after the uniform mixing of the medium for half an hour. The various germs occurred 50% and 100% at pH 8 and pH 7 of the non-sterilized medium, respectively. Production of oyster mushroom increased by $2,030\;ton\;ha^{-1}$ when the main raw material used corn pith instead of waste cotton. The time required of mycelium culture was 30 days when hypha was cultured at the non-sterilized medium, and pinhead occurred when 2 or 3 days was passed after the time required of mycelium culture. Occurrence of pinhead was most rapid at the condition of $22{\sim}26^{\circ}C$, 65% humidity and pH $6.5{\sim}7.0$ and required of $22{\sim}28$ days at $70{\sim}80\;mm$ thickness of non-sterilized medium. Ca content in 1st harvest oyster mushroom was higher than that in 2nd harvest one, and its difference was $30.3\;mg\;kg^{-1}$. Amino acid content by stipe thickness of oyster mushroom was ranged from 411.2 to $343.9\;mg\;kg^{-1}$ both in a pileus and a stipe of 1st harvest mushroom, and from 402.4 to $498.2\;mg\;kg^{-1}$ and from 442.6 to $470.4\;mg\;kg^{-1}$ in those of 2nd harvest one, respectively. The results of the present study suggest that the non-sterilization medium by addition of $Ca(OH)_2$ is usable with the cultivation of oyster mushroom.