• Journal of Periodontal and Implant Science
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• 제50권6호
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• pp.418-434
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• 2020

Purpose: The purpose of the present study was to evaluate the effect of silica-calcium phosphate composite (SCPC) granules on bone regeneration in extraction sockets. Methods: Ten patients were selected for a split-model study. In each patient, bone healing in SCPC-grafted and control ungrafted sockets was analyzed through clinical, radiographic, histomorphometric, and immunohistochemical assessments 6 months postoperatively. Results: A radiographic assessment using cone-beam computed tomography showed minimal ridge dimension changes in SCPC-grafted sockets, with 0.39 mm and 1.79 mm decreases in height and width, respectively. Core bone biopsy samples were obtained 6 months post-extraction during implant placement and analyzed. The average percent areas occupied by mature bone, woven bone, and remnant particles in the SCPC-grafted sockets were 41.3%±12%, 20.1%±9.5%, and 5.3%±4.4%, respectively. The percent areas of mature bone and woven bone formed in the control ungrafted sockets at the same time point were 31%±14% and 24.1%±9.4%, respectively. Histochemical and immunohistochemical analyses showed dense mineralized bundles of type I collagen with high osteopontin expression intensity in the grafted sockets. The newly formed bone was well vascularized, with numerous active osteoblasts, Haversian systems, and osteocytes indicating maturation. In contrast, the new bone in the control ungrafted sockets was immature, rich in type III collagen, and had a low osteocyte density. Conclusions: The resorption of SCPC granules in 6 months was coordinated with better new bone formation than was observed in untreated sockets. SCPC is a resorbable bone graft material that enhances bone formation and maturation through its stimulatory effect on bone cell function.

• 한국가스학회지
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• 제25권2호
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• pp.28-33
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• 2021

최근 친환경 자동차에 대한 관심이 높아짐에 따라 공해물질을 배출하지 않는 수소연료전지 차량에 대한 관심과 구매가 증가하고 있다. 최근 정부의 수소에너지 보급정책에 따라 수소충전소의 확충과 핵심부품의 국산화를 지원하고 있다. 본 연구에서는 그중에서도 수소 유량제어 밸브의 차압에 따른 유동특성을 연구하였다. 차압이 높아 짐에 따라 질량유량과 유량계수는 체적유량과 다른 경향을 보임을 확인하였다. 그리고 병목 구간에서의 노즐효과로 인해 수소온도에 영향을 주게되고, 밀도의 변화를 일으켜 질량유량에 영향을 주게 되는 것을 확인 하였다.

• 한국가스학회지
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• 제25권5호
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• pp.19-29
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• 2021

기후변화에 대응하기 위해 세계적으로 이산화탄소 및 대기오염 물질 배출 규제가 점차 강화됨에 따라 내연 기관 자동차를 친환경 자동차로 전환하려는 추세이다. 이는 정부의 수소 에너지 활성화 정책에 따른 충전 인프라 구축, 충전 설비 핵심 부품의 국산화 지원으로 가속화되고 있다. 본 연구에서는 유한 요소 해석을 통해 수소 연료 전지 자동차 용기 밸브의 과류 방지 장치를 설계하고, 시험을 통해 성능을 평가하였다. 주요 시험 항목인 정수압, 연속 작동, 압력 임펄스, 누설, 작동 시험을 ISO 12619-2, ISO 12619-11의 시험 방법에 따라 수행한 결과 요구 조건을 만족함을 확인하였다.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.449-458
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• 2019

Hepatitis B treatments using immune therapy are gaining interest because of the improvements in dendritic cell performance for antigen presentation, which induces an appropriate immune response and raises patient survival rates. This research aims to produce a significant amount of the HBcAg antigen, which can induce an immune response and have a curative effect on HBV infection. In this study, the HBV subgenotype B3 of the HBcAg gene was used, which is dominant in Indonesia. Further, Lactococcus lactis bacteria was used as the host because of its safety and tightly regulated protein expression. The codon usage for the HBcAg gene was optimized to improve protein expression in L. lactis, which is important because a codon is not random between species. The HBcAg gene is attached to a pNZ8148 plasmid and transformed into the L. lactis NZ3900 expression host. The results confirm that a positive protein band (21 kDa) in two fractions of purified HBcAg was recognized by both western blotting and dot blot hybridization, even if the HBcAg optimized codon has higher GC contents than that suggested for L. lactis expression. Overall, this research strengthens the broad use of L. lactis bacteria for any protein expression, including higher protein expression of codon optimized HBcAg gene compared to non-optimized genes. Furthermore, the improvement in the codon optimization of the HBcAg gene significantly increases the total protein expression by 10-20%, and the expression level of the codon optimized HBcAg increases 1.5 to 3.2-times that of the native HBcAg.

• 동의생리병리학회지
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• 제34권4호
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• pp.177-183
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• 2020

The Hippo-YAP signaling pathway is critical for cell proliferation, survival, and self-renewal in both Drosophila and mammals. Disorder of Hippo-YAP pathway leads to tumor development, progression and poor prognosis in various cancers. YAP/TAZ are the key downstream effectors of the Hippo pathway and they can be inhibited through LATS1/2, core kinases in the Hippo pathway, mediated phosphorylation. In this study, we investigated the effect of Angelica gigas Nakai extract (AGNE) on Hippo-YAP/TAZ pathway. First, ANGE induced YAP/TAZ phosphorylation and dissociation of the YAP/TAZ-TEAD transcription complex. By qRT-PCR, we found that ANGE inhibits the expression of YAP/TAZ-TEAD target gene, CTGF and CYR61. In addition, the transcriptional activity of YAP/TAZ was not suppressed significantly in LATS1/2 double-knockout (DKO) cells by ANGE compared to LATS1/2 wild-type (WT) cells, which means AGNE inhibits YAP/TAZ signaling through direct action on LATS1/2. Further, it was confirmed that AGNE-induced activation of LATS1/2 inhibited the migration potential of the vector-expressing cells by suppressing YAP/TAZ activity. The reduced migration potential was restored in active YAP-TEAD expressing cells. Taken together, the results of this study indicate that ANGE downregulates YAP/TAZ signaling in cells through the activation of LATS1/2.

• 한국정보통신학회논문지
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• 제25권3호
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• pp.419-426
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• 2021

NIST FIPS 186-2에 정의된 GF(p) 상의 5 가지 체 크기 (192, 224, 256, 384, 521 비트)와 8 가지의 산술연산 동작모드 (ECPSM, ECPA, ECPD, MA, MS, MM, MI, MD)를 지원하는 고성능 타원곡선 암호 프로세서 HP-ECCP를 설계하였다. HP-ECCP가 부채널 공격에 내성을 갖도록 만들기 위해, 타원곡선 점 스칼라 곱셈에 사용되는 개인키의 해밍웨이트에 무관하게 점 덧셈과 점 두배 연산이 균일하게 수행되는 수정된 left-to-right 이진 알고리듬을 적용하여 설계했다. 또한, 타원곡선 점 연산에 핵심이 되는 모듈러 곱셈 연산의 고성능 하드웨어 구현을 위해 Karatsuba-Ofman 곱셈 알고리듬, Lazy 축약 알고리듬, Nikhilam 나눗셈 알고리듬을 적용하여 설계했다. HP-ECCP를 180 nm CMOS 표준 셀 라이브러리로 합성한 결과 67 MHz의 동작 주파수에서 620,846 등가 게이트로 구현되었으며, 체 크기 256 비트의 ECPSM이 초당 2,200회 계산될 수 있는 것으로 평가되었다.

• Journal of Animal Science and Technology
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• 제64권4호
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• pp.707-716
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• 2022

The core intention to undertake this experiment for a period of 21 days is to evaluate the effect of salicylic acid (SA) supplemented diet on the performance and blood metabolites of sows and their litters. Sows weighing 208.5 ± 18.34kg and their neonates were used. From day 114 of gestation to 21st day of lactation(weaning), ten multiparous sows (n = 5/treatment) (Landrace × Yorkshire) were assigned randomly into one of two treatments: CON (basal diet) and TRT (CON + 0.05% SA). There was no significant difference in the body weight, backfat thickness, backfat loss, and body condition score in SA treated sows compared to sows fed the CON diet. However, the bodyweight of sow was dramatically reduced by an average of 16kg from farrowing to weaning time. The dietary inclusion of SA in the sow diet slightly improved the survival rate (p = 0.065) and showed a higher body weight (p = 0.009) in piglets. However, there was no significant difference in red blood cell, Fe, Hematocrit, and Hb concentrations between CON and TRT sows' groups, but the total iron-binding capacity (TIBC) was significantly reduced in sows from the TRT group compared with the CON group from the beginning to weaning. The outcome of this trial shows that dietary addition of SA on sows diet from early lactation could increase the birth weight and TIBC of neonates at the end of the trial.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제12권1호
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• pp.1-8
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• 2023

SSD는 이미 할당된 페이지에 대한 제자리 덮어쓰기가 불가능하므로 쓰기 연산이 있을 때마다 새로운 클린 페이지로의 대체가 필요하다. 이 문제를 지원하기 위해 SSD는 운영체제의 파일시스템에서 관리하는 논리 페이지를 현재 할당된 물리 페이지로 매핑하는 플래시 변환 계층인 FTL을 내부에 둔다. 쓰기 연산으로 버려진 SSD 페이지는 초기화 작업을 거쳐 재활용되어야 하는데, 그 횟수에 제한이 있기 때문에 FTL은 기본인 페이지 매핑 기능 외에 쓰기 횟수를 줄일 수 있는 캐시 기능을 제공한다. 이 연구에서는 쓰기 횟수를 줄이기 위한 FTL의 캐시 방법론에 집중하여 관련된 알고리즘들을 분석하고, 쓰기 전용 캐시 전략을 제안한다. 시뮬레이터를 사용하여 쓰기 전용 캐시를 실험한 결과 최대 29%의 개선 효과를 보였다.

• 대한한의학방제학회지
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• 제32권1호
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• pp.11-28
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• 2024

Objectives : GunRyeong-Tang(GRT) is a traditional herbal prescription that combines Oryeongsan and Sagunja-tang. This study employed network analysis methods on the components of GRT and target genes related to diabetes complications to predict the improvement effects of GRT on diabetes complications. Methods : The collection of active compounds of GRT and related target genes involved the utilization of public databases and the PubChem database. We selected diabetes complication-related genes using GeneCards and confirmed their correlation through comparative analysis with the target genes of GRT. We constructed a network using Cytoscape 3.9.1 and conducted topological analysis. To predict the mechanism, we performed functional enrichment analysis based on Gene Ontology (GO) biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Results : Through network analysis, 234 active compounds and 1361 related genes were collected from GRT. A total of 9,136 genes related to diabetes complications were collected, and 1,039 target genes overlapping with the components of GRT were identified. The core genes of this network were TP53, INS, AKT1, ALB, and EGFR. In addition, GRT significantly reduced the H9c2 cell size and the expression of myocardial hypertrophy biomarkers (ANP, BNP), which were increased by high glucose (HG). Conclusions : Through this study, we were able to predict the activity and mechanism of action of GRT on diabetes and diabetic complications, and confirmed the potential of GRT as a treatment for diabetes complications through the effect of GRT on improving myocardial hypertrophy for diabetic cardiomyopathy.

• IMMUNE NETWORK
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• 제22권3호
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• pp.21.1-21.21
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• 2022

As far the current severe coronavirus disease 2019 (COVID-19), respiratory disease is still the biggest threat to human health. In addition, infectious respiratory diseases are particularly prominent. In addition to killing and clearing the infection pathogen directly, regulating the immune responses against the pathogens is also an important therapeutic modality. Sirtuins belong to NAD+-dependent class III histone deacetylases. Among 7 types of sirtuins, silent information regulator type-1 (SIRT1) played a multitasking role in modulating a wide range of physiological processes, including oxidative stress, inflammation, cell apoptosis, autophagy, antibacterial and antiviral functions. It showed a critical effect in regulating immune responses by deacetylation modification, especially through high-mobility group box 1 (HMGB1), a core molecule regulating the immune system. SIRT1 was associated with many respiratory diseases, including COVID-19 infection, bacterial pneumonia, tuberculosis, and so on. Here, we reviewed the latest research progress regarding the effects of SIRT1 on immune system in respiratory diseases. First, the structure and catalytic characteristics of SIRT1 were introduced. Next, the roles of SIRT1, and the mechanisms underlying the immune regulatory effect through HMGB1, as well as the specific activators/inhibitors of SIRT1, were elaborated. Finally, the multitasking roles of SIRT1 in several respiratory diseases were discussed separately. Taken together, this review implied that SIRT1 could serve as a promising specific therapeutic target for the treatment of respiratory diseases.