• Korean Journal of Remote Sensing
• /
• v.20 no.2
• /
• pp.125-137
• /
• 2004

In case of using image sequence taken from a moving camera along a road in an urban area, general video mosaicing technique based on a single baseline cannot create 2-D image mosaics. To solve the drawback, this paper proposed a new image mosaicing technique through 3-D multi-baselines that can create image mosaics in 3-D space. The core of the proposed method is that each image frame has a dependent baseline, an equation of first order, calculated by using ground control point (GCP) of optical flows. The proposed algorithm consists of 4 steps: calculation of optical flows using hierarchical strategy, calculation of camera exterior orientation, determination of multi-baselines, and seamless image mosaics. This paper realized and showed the proposed algorithm that can create efficient image mosaics in 3-D space from real image sequence.

• Microbiology and Biotechnology Letters
• /
• v.47 no.1
• /
• pp.25-33
• /
• 2019

Lactobacillus rhamnosus BFE5264, isolated from a Maasai fermented milk product ("kule naoto"), was previously shown to exhibit bile acid resistance, cholesterol assimilation, and adhesion to HT29-MTX cells in vitro. In this study, we re-annotated and analyzed the previously reported complete genome sequence of strain BFE5264. The genome consists of a circular chromosome of 3,086,152 bp and a putative plasmid, which is the largest one identified among L. rhamnosus strains. Among the 2,883 predicted protein-coding genes, those with carbohydrate-related functions were the most abundant. Genome analysis of strain BFE5264 revealed two consecutive CRISPR regions and no known virulence factors or antimicrobial resistance genes. In addition, previously known highly variable regions in the genomes of L. rhamnosus strains were also evident in strain BFE5264. Pairwise comparison with the most studied probiotic strain L. rhamnosus GG revealed strain BFE5264-specific deletions, probably due to insertion sequence-mediated recombination. The latter was associated with loss of the spaCBA pilin gene cluster and exopolysaccharide biosynthetic genes. Comparative genomic analysis of the sequences from all available L. rhamnosus strains revealed that they were clustered into two groups, being within the same species boundary based on the average nucleotide identities. Strain BFE5264 had a sister group relationship with the group that contained strain GG, but neither ANI-based hierarchical clustering nor core-gene-based phylogenetic tree construction showed a clear distinctive pattern associated with the isolation source, implying that the genotype alone cannot account for their ecological niches. These results provide insights into the probiotic mechanisms of strain BFE5264 at the genomic level.

• Journal of the Korea Convergence Society
• /
• v.10 no.5
• /
• pp.165-171
• /
• 2019

Engineering and Technology have been influencing a lot in the field of sports. Competitiveness, attributes of sports, have forced not only sports players but sports goods to enhance those performance. Particularly in the field of motorsports, the convergence of sports and technology has long been done to satisfy between performance and safety. In this study, strength analysis was carried with FEM to develop CFRP Laminate(Carbon Fiber Reinforced Plastic Laminate) bucket seat targeted to motorsports and car tuning industries and FIA($F\acute{e}d\acute{e}ration$ Internationale de l'Automobile) regulation was applied to design the racing seat and evaluate its strength. FEM modeling considered the attributes of composites was followed by strength evaluation based on Tsai-Wu failure index were done according to Lay-up sequence and layer numbers. The result showed that the lay-up sequence with stacking angle such as $[0^{\circ}/30^{\circ}/60^{\circ}/90^{\circ}/-30^{\circ}/-60^{\circ}]_4$ with 3mm form core was optimal selection in the field of weight and strength evaluation.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.7
• /
• pp.875-885
• /
• 2023

Volatile organic compounds such as benzene, toluene, ethylbenzene, and isomers of xylenes (BTEX) constitute a group of monoaromatic compounds that are found in petroleum and have been classified as priority pollutants. In this study, based on its newly sequenced genome, we reclassified the previously identified BTEX-degrading thermotolerant strain Ralstonia sp. PHS1 as Cupriavidus cauae PHS1. Also presented are the complete genome sequence of C. cauae PHS1, its annotation, species delineation, and a comparative analysis of the BTEX-degrading gene cluster. Moreover, we cloned and characterized the BTEX-degrading pathway genes in C. cauae PHS1, the BTEX-degrading gene cluster of which consists of two monooxygenases and meta-cleavage genes. A genome-wide investigation of the PHS1 coding sequence and the experimentally confirmed regioselectivity of the toluene monooxygenases and catechol 2,3-dioxygenase allowed us to reconstruct the BTEX degradation pathway. The degradation of BTEX begins with aromatic ring hydroxylation, followed by ring cleavage, and eventually enters the core carbon metabolism. The information provided here on the genome and BTEX-degrading pathway of the thermotolerant strain C. cauae PHS1 could be useful in constructing an efficient production host.

• Composites Research
• /
• v.19 no.2
• /
• pp.13-19
• /
• 2006

In this paper, parametric study was carried out to design sandwich structures for EDM machines controlling stacking sequence, stacking thickness of composites and rib configuration. Sandwich structures which are dealt with in this paper are composed of fibre reinforced composite for skin material and foam or resin concrete for core materials. The sandwich column has cruciform rib to enhance bending stiffness of the structure and the bed has several vertical ribs to resist the normal forces and vibration. The design parameters such as rib thickness and stacking sequence were controlled to enhance the system robustness. Finite element analysis was also carried out to verify the variation of static and dynamic stiffness of the structures according to the variation of the parameters. Vibration tests were performed to verify the natural frequencies and damping ratios of the manufactured composite structures. The appropriate shape and configuration conditions for micro-EDM machine structures are proposed.

• Journal of the Korean Magnetic Resonance Society
• /
• v.6 no.1
• /
• pp.54-68
• /
• 2002

PEBP2/CBF (Polyomavirus Enhancer-core Binding Protein 2/Core Binding Factor), represents a new family of heterodimeric transcription factor. Those members play important roles in hematopoiesis and osteogenesis in mouse and human. PEBP2/CBF is a sequence-specific DNA binding protein. Each member of the PEBP2/CBF family of transcription factors is composed of two subunits, ${\alpha}$ and ${\beta}$. The evolutionarily conserved 128 amino acid region in ${\alpha}$ subunit has been called the Runt domain, which harbors two different activities, the ability to bind DNA and interact with the ${\beta}$ subunit. Recently, cDNA clones encoding the C. elegans Runt domain were isolated by screening a cDNA library. This gene was referred to run (Runt homologous gene). In this study, the basic experiments for the structural characterization of RUN protein were performed using spectroscopic methods. We have identified the structural properties of RUN using bioinformatics, CD and NMR. The limit temperature of the structural stability was up to 60$^{\circ}C$ with irreversible thermal process, and the structure of RUN seems to adopt ${\alpha}$ helices and one or more ${\beta}$ sheet or turn. The degree of NMR peak dispersion and intensity was increased by addition of glycine. Therefore, glycine could be used to alleviate the aggregation property of RUN in NMR experiment.

• Journal of Korean Neurosurgical Society
• /
• v.41 no.5
• /
• pp.306-313
• /
• 2007

Objective : In permanent distal middle cerebral artery occlusion [pdMCAO] model of rats, the temporal order of subcellular translocation is not fully understood yet. We studied translocation sequence of cytochrome c and apoptosis inducing factor [AIF] after pdMCAO and patterns of expression. Methods : Twenty-one male rats - with ten minutes, 1, 4, 8, 24 and 48 hours of pdMCAO groups - were enrolled. At core and penumbra area of each cerebral cortex, Western blotting of cytochrome c and AIF were performed using cytosolic fractions and then compared with sham specimens. With 48 hours group, the expression of cytochrome c and AIF was examined with immunofluorescent staining. Results : Compared to sham, the cytosolic translocation of cytochrome c significantly increased at all time points [p<0.05]. As early as 10 min after onset of ischemia, it was increased significantly [p<0.01]. The cytosolic translocation of AIF showed gradual increase with the passage of time and significantly increased 8 hours after [p<0.05]. As late as 24 hours and 48 hours after onset of ischemia, there were increased most significantly [p<0.01]. At penumbra, both proteins failed to show significant increase at all time points. At 48 hours after ischemia, colocalization of cytochrome c and AIF were confirmed. Conclusion : Cytosolic translocation of cytochrome c peaks much earlier than that of AIF in pdMCAO model of rat. Caspase dependent apoptosis activates soon after ischemia and later, it can be reinforced by gradually increasing AIF in ischemic core.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.57 no.3
• /
• pp.239-252
• /
• 2024

Recombination activating genes (RAGs) play a crucial role in initiating V(D)J recombination, which is essential for developing adaptive immunity in vertebrates. In this study, we cloned and characterized RAG1/2 cDNA from the marine medaka Oryzias dancena (OdRAG1/2) and investigated their mRNA expression patterns during ontogenetic developmental stages. The OdRAG1 and OdRAG2 cDNA contained open reading frames (ORFs) encoding proteins containing 1,078 and 531 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that OdRAG1 and OdRAG2 are highly conserved with their corresponding orthologs, featuring distinct core and non-core regions. Notably, expression analysis showed that, in contrast to other fish RAGs studied, OdRAG1/2 expression peaked at 0 days post-hatching (DPH). Additionally, for the expression of T and B cell differentiation markers, CD3γ and CD20, also peaked at 0 DPH. Collectively, adaptive immunity in O. dancena potentially begins during embryonic development, which is critical for V(D)J recombination and essential immune component development, suggesting the early ontogenetic stage interactions between innate and adaptive immunity.

• Nuclear Engineering and Technology
• /
• v.56 no.9
• /
• pp.3571-3584
• /
• 2024

Reactor core transient calculation is very important for the reactor safety analysis, in which the kernel is neutron kinetics calculation by simulating the variation of neutron density or thermal power over time. Compared with the point kinetics method, the time-space neutron kinetics calculation can provide accurate variation of neutron density in both space and time domain. But it consumes a lot of resources. It is necessary to develop a surrogate model that can quickly obtain the temporal and spatial variation information of neutron density or power with acceptable calculation accuracy. This paper uses the time-varying characteristics of power to construct a time function, parameterizes the time-varying characteristics which contains the information about the spatial change of power. Thereby, the amount of targets to predict in the space domain is compressed. A surrogate method using the machine learning is proposed in this paper. In the construction of a neural network, the input is processed by a convolutional layer, followed by a fully connected layer or a deconvolution layer. For the problem of time sequence disturbance, a structure combining convolutional neural network and recurrent neural network is used. It is verified in the tests of a series of 1D, 2D and 3D reactor models. The predicted values obtained using the constructed neural network models in these tests are in good agreement with the reference values, showing the powerful potential of the surrogate models.

• Korean Journal of Ichthyology
• /
• v.19 no.4
• /
• pp.266-273
• /
• 2007

Heat shock proteins (HSPs) are a family of highly conserved proteins playing an important role in the functioning of unstressed and stressed cells. The HSP70 family, the most widely studied of the hsps, is constitutively expressed (hsc70) in unstressed cells and is also induced in response to stressors (hsp70), especially those affecting the protein machinery. The HSP/HSC70 proteins act as molecular chaperones and are crucial for protein functioning, including folding, intracellular localization, regulation, secretion, and protein degradation. Here, we report the identification and characterization of the putative amino acid sequence deduced from one cDNA clone identified as heat shock protein 70. The alignment showed that the putative sequence is 100% identical to the heat shock protein 70 cognate (HSC 70) of olive flounder. The 5'-flanking region sequence (approximately 1 kb) ahead of the hsc70 gene was cloned by genome walking and a putative core promoter region and transcription elements were identified. We characterized the promoter of the olive flounder hsc70 gene by examining the ability of 5'-upstream fragments to drive expression of green fluorescent protein (GFP) in live embryos.