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Expression of GFP Gene Driven by the Olive Flounder (Paralichthys olivaceus) hsc70 Promoter in Trangenic Medaka (Oryzias latipes)  

Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Jong-Hyun (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Noh, Jae Koo (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Hyun Chul (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Woo-Jin (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Kim, Young-Ok (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Kim, Kyung-Kil (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Publication Information
Korean Journal of Ichthyology / v.19, no.4, 2007 , pp. 266-273 More about this Journal
Abstract
Heat shock proteins (HSPs) are a family of highly conserved proteins playing an important role in the functioning of unstressed and stressed cells. The HSP70 family, the most widely studied of the hsps, is constitutively expressed (hsc70) in unstressed cells and is also induced in response to stressors (hsp70), especially those affecting the protein machinery. The HSP/HSC70 proteins act as molecular chaperones and are crucial for protein functioning, including folding, intracellular localization, regulation, secretion, and protein degradation. Here, we report the identification and characterization of the putative amino acid sequence deduced from one cDNA clone identified as heat shock protein 70. The alignment showed that the putative sequence is 100% identical to the heat shock protein 70 cognate (HSC 70) of olive flounder. The 5'-flanking region sequence (approximately 1 kb) ahead of the hsc70 gene was cloned by genome walking and a putative core promoter region and transcription elements were identified. We characterized the promoter of the olive flounder hsc70 gene by examining the ability of 5'-upstream fragments to drive expression of green fluorescent protein (GFP) in live embryos.
Keywords
Olive flounder (Paralichthys olivaceus); hsc70 promoter; green fluorescent protein (GFP);
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1 Dworniczak, B. and M.E. Mirault. 1987. Structure and expression of a human gene coding for a 71 kd heat shock 'cognate' protein. Nucleic Acids Res., 15 : 5181-5197   DOI   ScienceOn
2 Lindquist, S. and E.A. Craig. 1988. The heat-shock proteins. Annu. Rev. Genet., 22 : 631-677   DOI   ScienceOn
3 Ohsako, S., D. Bunick and Y. Hayashi. 1995. Immunocytochemical observation of the 90 KD heat shock protein (HSP90): high expression in primordial and pre-meiotic germ cells of male and female rat gonads. J. Histochem. Cytochem., 43 : 67-76   DOI   ScienceOn
4 Yang, T. and B.W. Poovaiah. 2002. A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants. J. Biol. Chem., 277 : 45049-45058   DOI   ScienceOn
5 Boorstein, W.R., T. Ziegelhoffer and E.A. Craig. 1994. Molecular evolution of the HSP70 multigene family. J. Mol. Evol., 38 : 1-17
6 Arai, A., K. Naruse, H. Mitani and A. Shima. 1995. Cloning and characterization of cDNAs for 70-kDa heat-shock proteins (Hsp70) from two fish species of the genus Oryzias. Jpn. J. Genet., 70 : 423-433   DOI
7 Lin, S., W. Long, J. Chen and N. Hopkins. 1992. Production of germ-line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos. Proc. Natl. Acad. Sci. USA, 89 : 4519-4523
8 Mayer, M.P. and B. Bukau. 1998. Hsp70 chaperone systems: diversity of cellular functions and mechanism of action. Biol. Chem., 379 : 261-268
9 White, C.N., L.E. Hightower and R.J. Schultz. 1994. Variation in heat-shock proteins among species of desert fishes (Poeciliidae, Poeciliopsis). Mol. Biol. Evol., 11 :106-119
10 Zafarullah, M., J. Wisniewski, N.W. Shworak, S. Schieman, S. Misra and L. Gedamu. 1992. Molecular cloning and characterization of a constitutively expressed heatshock-cognate hsc71 gene from rainbow trout. Eur. J. Biochem., 204 : 893-900   DOI   ScienceOn
11 Santacruz, H., S. Vriz and N. Angelier. 1997. Molecular characterization of a heat shock cognate cDNA of zebrafish, hsc70, and developmental expression of the corresponding transcripts. Dev. Genet., 21 : 223-233   DOI   ScienceOn
12 Kothary, R.K., D. Jones and E.P. Candido. 1984. 70-kDa heat shock polypeptides from rainbow trout: characterization of cDNA sequences. Mol. Cell Biol., 4 : 1785-1791   DOI
13 Bukau, B. and A.L. Horwich. 1998. The Hsp70 and Hsp60 chaperone machines. Cell, 92 : 351-366   DOI   ScienceOn
14 Place, S.P. and G.E. Hofmann. 2001. Temperature interactions of the molecular chaperone Hsc70 from the eurythermal marine goby Gillichthys mirabilis. J. Exp. Biol., 204 : 2675-2682
15 Paul, C. and A.P. Arrigo. 2000. Comparison of the protective activities generated by two survival proteins: Bcl-2 and Hsp27 in L929 murine fibroblasts exposed to menadione or staurosporine. Exp. Gerontol., 35 : 757-766   DOI   ScienceOn
16 Graser, R.T., D. Malnar-Dragojevic and V. Vincek. 1996. Cloning and characterization of a 70-kDa heat shock cognate (hsc70) gene from the zebrafish (Danio rerio). Genetica, 98 : 273-276   DOI
17 Craig, E.A., T.D. Ingolia and L.J. Manseau. 1983. Expression of Drosophila heat-shock cognate genes during heat shock and development. Dev. Biol., 99 : 418-426   DOI   ScienceOn
18 Hartl, U.F. 1996. Molecular chaperones in cellular protein folding. Nature, 381 : 571-580   DOI   ScienceOn
19 Molina, A., F. Biernar, F. Muller, A. Iyengar, P. Prunet, N. Maclean, J.A. Martial and M. Muller. 2000. Cloning and expression analysis of an inducible HSP70 gene from tilapia fish. FEBS Lett., 474 : 5-10   DOI   ScienceOn
20 Norris, C.E., P.J. di Iorio, R.J. Schultz and L.E. Hightower. Variation in heat shock proteins within tropical and desert species of poeciliid fishes. Mol. Biol. Evol., 12 :1048-1062
21 Bensaude, O., C. Babinet, M. Morange and F. Jacob. 1983. Heat shock proteins, first major products of zygotic gene activity in mouse embryo. Nature, 305 : 331-333   DOI   ScienceOn
22 Niwa, K., T. Ladygina, M. Kinoshita, K. Ozato and Y. Wakamatsu. 1999. Transplantation of blastula nuclei to non-enucleated eggs in the medaka, Oryzias latipes. Develop. Growth Differ., 41 : 163-172   DOI   ScienceOn