• Journal of Microbiology and Biotechnology
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• 제33권5호
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• pp.591-599
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• 2023

Fisetin is a bioactive flavonol molecule and has been shown to have antioxidant potential, but its efficacy has not been fully validated. The aim of the present study was to investigate the protective efficacy of fisetin on C2C12 murine myoblastjdusts under hydrogen peroxide (H₂O₂)-induced oxidative damage. The results revealed that fisetin significantly weakened H₂O₂-induced cell viability inhibition and DNA damage while blocking reactive oxygen species (ROS) generation. Fisetin also significantly alleviated cell cycle arrest by H₂O₂ treatment through by reversing the upregulation of p21WAF1/CIP1 expression and the downregulation of cyclin A and B levels. In addition, fisetin significantly blocked apoptosis induced by H₂O₂ through increasing the Bcl-2/Bax ratio and attenuating mitochondrial damage, which was accompanied by inactivation of caspase-3 and suppression of poly(ADP-ribose) polymerase cleavage. Furthermore, fisetin-induced nuclear translocation and phosphorylation of Nrf2 were related to the increased expression and activation of heme oxygenase-1 (HO-1) in H₂O₂-stimulated C2C12 myoblasts. However, the protective efficacy of fisetin on H₂O₂-mediated cytotoxicity, including cell cycle arrest, apoptosis and mitochondrial dysfunction, were greatly offset when HO-1 activity was artificially inhibited. Therefore, our results indicate that fisetin as an Nrf2 activator effectively abrogated oxidative stress-mediated damage in C2C12 myoblasts.

• BMB Reports
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• 제37권2호
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• pp.192-198
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• 2004

The hepatitis C virus (HCV) core protein is believed to be one of viral proteins that are capable of preventing virus-infected cell death upon various stimuli. But, the effect of the HCV core protein on apoptosis that is induced by various stimuli is contradictory. We examined the possibility that the HCV core protein affects the ceramide-induced cell death in cells expressing the HCV core protein through the sphingomyelin pathway. Cell death that is induced by $C^2$-ceramide and bacterial sphingomyelinase was analyzed in 293 cells that constitutively expressed the HCV core protein and compared with 293 cells that were stably transfected only with the expression vector. The HCV core protein inhibited the cell death that was induced by these reagents. The protective effects of the HCV core protein on ceramide-induced cell death were reflected by the reduced expression of $p21^{WAF1/Cip1/Sid1}$ and the sustained expression of the Bcl-2 protein in the HCV core-expressing cells with respect to the vector-transfected cells. These results suggest that the HCV core protein in 293 cells plays a role in the modulation of the apoptotic response that is induced by ceramide. Also, the ability of the HCV core protein to suppress apoptosis might have important implications in understanding the pathogenesis of the HCV infection.

• 한국기계가공학회지
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• 제13권5호
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• pp.70-75
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• 2014

Permanent magnets have recently been considered as device that can be used to control the behavior of mechanical systems. Neodymium magnets, a type of permanent magnet, have been used in numerous mechanical devices. These are permanent magnets made from an alloy of neodymium, iron, and boron to form the Nd2Fe14B tetragonal crystalline structure. The magnetic selection, magnet core design and mechanical errors of the magnetic component can affect the performance of the magnetic force. In this study, the coercive force, residual induction, and the dimensions of the design parameters of the magnet core are optimized. The design parameters of magnet core are defined as the gap between the magnet and the core, the upper contact radius, and the lower thickness of the core. The force exercised on a permanent magnet in a non-uniform field is dependent on the magnetization orientation of the magnet. Non-uniformity of the polarization direction of the magnetic has been assumed to be caused by the angular error in the polarization direction. The variation in the magnetic performance is considered according to the center distance, the tilt of the magnetic components, and the polarization direction. The finite element method is used to analyze the magnetic force of an optimized cylindrical magnet.

• 마이크로전자및패키징학회지
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• 제20권4호
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• pp.69-74
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• 2013

In this paper, the impact of integrating large number of I/O (Input-Output) and Core power Delivery Network (PDN) on a 6 layers Flip-Chip Ball Grid Array (FCBGA) package is investigated. The impact of core induced supply noise on high-speed I/O interfaces, and high-speed I/O interface's supply noise coupling to adjacent high-speed I/O interfaces' jitter impact are studied. Concurrent stress validation software is used to induce SSO noise on each individual I/O interfaces; and at the same time; periodic noise is introduced from Core PDN into the I/O PDN domain. In order to have the maximum coupling impact, a prototype package is designed to merge the I/O and Core PDN as one while impact on jitter on each I/O interfaces are investigated. In order to understand the impact of the Core to I/O and I/O to I/O noise, the on-die noise measurements were measured and results were compared with the original PDN where each I/O and Core PDN are standalone and isolated are used as a benchmark.

• 한국압력기기공학회 논문집
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• 제15권2호
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• pp.58-63
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• 2019

This paper presents preliminary analysis and results on IASCC sensitivity of a core shroud in the reactor pressure vessel. First, neutron irradiation flux distribution of the reactor internals was calculated by using the Monte Carlo simulation code, MCNP6.1 and the nuclear data library, ENDF/B-VII.1. Second, based on the neutron irradiation flux distribution, temperature and stress distributions of the core shroud during normal operation were determined by performing finite element analysis using the commercial finite element analysis program, ABAQUS, considering irradiation aging-related degradation mechanisms. Last, IASCC sensitivity of the core shroud was assessed by using the IASCC sensitivity definition of EPRI MRP-211 and the finite element analysis results. As a result of the preliminary analysis, it was found that the point at which the maximum IASCC sensitivity is derived varies over operating time, initially moving from the shroud plate located in the center of the core to the top shroud plate-ring connection brace over operating time. In addition, it was concluded that IASCC will not occur on the core shroud even after 60 years of operation (40EFPYs) because the maximum IASCC sensitivity is less than 0.5.

• 한국전기전자재료학회논문지
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• 제34권6호
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• pp.454-460
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• 2021

In this work, we synthesized alloy-core InZnP quantum dots, which are more efficient than single-core InP quantum dots, using a solution process method. The effect of synthesis conditions of alloy core on optical properties was investigated. We also investigated the conditions that make up the gradient shell to minimize defects caused by lattice mismatch between the InZnP core and ZnS is 7.7%. The stable synthesis temperature of the InZnP alloy core was 200℃. Quantum dots consisting of three layered ZnSe gradient shell and single layered ZnS exhibited the best optical property. The properties of quantum dots synthesized in 100 ml and in 2,000 ml flasks were almost equal.

• Korean Journal of Radiology
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• 제24권11호
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• pp.1164-1166
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• 2023

• 천문학회지
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• 제43권1호
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• pp.9-23
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• 2010

In this paper we examined the association of Infrared Dark Cloud (IRDC) cores with YSOs and the geometric properties of the IRDC cores. For this study a total of 13,650 IRDC cores were collected mainly from the catalogs of the IRDC cores published from other studies and partially from our catalog of IRDC cores containing new 789 IRDC core candidates. The YSO candidates were searched for using the GLIMPSE, MSX, and IRAS point sources by the shape of their SED or using activity of water or methanol maser. The association of the IRDC cores with these YSOs was checked by their line-of-sight coincidence within the dimension of the IRDC core. This work found that a total of 4,110 IRDC cores have YSO candidates while 9,540 IRDC cores have no indication of the existence of YSOs. Considering the 12,200 IRDC cores within the GLIMPSE survey region for which the YSO candidates were determined with better sensitivity, we found that 4,098 IRDC cores (34%) have at least one YSO candidate and 1,072 cores among them seem to have embedded YSOs, while the rest 8,102 (66%) have no YSO candidate. Therefore, the ratio of [N(IRDC core with protostars)]/[N(IRDC core without YSO)] for 12,200 IRDC cores is about 0.13. Taking into account this ratio and typical lifetime of high-mass embedded YSOs, we suggest that the IRDC cores would spend about $10^4\sim10^5$ years to form high-mass stars. However, we should note that the GLIMPSE point sources have a minimum detectable luminosity of about $1.2 L_{\odot}$ at a typical IRDC core's distance of ~4 kpc. Therefore, the ratio given here should be a 100ver limit and the estimated lifetime of starless IRDC cores can be an upper limit. The physical parameters of the IRDC cores somewhat vary depending on how many YSO candidates the IRDC cores contain. The IRDC cores with more YSOs tend to be larger, more elongated, and have better darkness contrast than the IRDC cores with fewer or no YSOs.

• 생명과학회지
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• 제17권4호
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• pp.476-481
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• 2007

꾸지뽕나무(Cudrania tricuspidata(Carr.) Bureau) 뿌리껍질에서 3종의 xanthones 화합물을 분리하였으며, 분광학적인 자료를 바탕으로 cudraxanthone L (1), cudraxanthone D (2), 그리고 cudraxanthone M (3)으로 구조동정되었다. 분리된 화합물을 L-tyrosine이 기질로 작용할 때 tyrosinase 저해 활성을 측정한 결과 cudraxanthone M (3)의 $IC_{50}$ 값이 $16.5{\mu}M$로 가장 높은 저해 효능을 보였으며, kinetic type은 uncompetitive inhibition로 저해 상수($K_i$)는 $1.6{\mu}M$로 측정되었다. 또한 이 화합물은 $20{\mu}M$의 농도에서 lag time이 310초로 측정 되어 대조화합물인 kojic acid와 유사한 저해능을 나타내었다.

• Journal of Magnetics
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• 제21권3호
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• pp.450-459
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• 2016

Experimental results and data of numerical simulations are presented, concerning generation of wideband radio frequency (RF) oscillations in a nonlinear transmission line (NLTL) which contains a pre-magnetized core of ferrite material. Emphasis is made on the means for extracting the RF signal from the line, in order to radiate it into free space. Antennas of two types that can be used for the purpose are considered, both featuring a coaxial design. This permits availing of the principal advantages of coaxial systems, such as operation in the mode of a traveling TEM wave; wide range of the transmitted frequencies, and a reasonably simple design. The antennas studied, specifically a disc-cone dipole (DCD) and an impulse reflector antenna ('Half-IRA', or HIRA type) differ significantly in effective width of the radiated spectrum and in spatial characteristics of the radiated field in far region.