• Korean Journal of Microbiology
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• v.29 no.5
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• pp.267-269
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• 1991

The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.25-27
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• 1999

Degenerate primers corresponding to the sequences of the copper-binding regions in the fungal laccases were used to isolatc laccase gene specific fragment by PCR in Coprinus congregahts. A 144 bp DNA hagrnent was cloned and was identified to have 60-69 % homology with other fungal laccase genes. The predicted amino acid sequcnces showed 68-75% homology with other fungal laccase proteins.

• Korean Journal of Microbiology
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• v.33 no.1
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• pp.27-30
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• 1997

Coprinus congregatus를 pH 4.2로 낮춘 YpSs 액체배지에 접종할 경우 세포막연관효소인 laccase가 배양 1일만에 상등액으로 대량 생성분비된다. 분비된 효소는 매우 빠르게 효소력의 감소를 보이며 전기영동상의 이동변화가 있다. 이와 같이 변화된 전기영동상을 보이는 효소단백질을 분광광도계로 분석할 경우 정제된 효소단백질과는 다른 분광 스펙트럼을 보인다.

• Korean Journal of Microbiology
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• v.28 no.3
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• pp.274-277
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• 1990

The hyphal tip phenoloxidases of Coprinus congregatus were localized by the protoplast-concanavalin A method. Protoplast were generated from cultures grown on a solid medium which was solidified with a new gelling agent, Pluronid Polyol F127, instead of agar. the enzymes were associated with the cell membrane which might work as a transducer in the light recepter complex.

• Korean Journal of Microbiology
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• v.33 no.4
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• pp.274-276
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• 1997

Transformation of Coprinus congregatus with a linearized plasmid has been successfully carried out using phosphinothricin resistance gene as a dominant selectable marker. The transforming frequency was about 500 transformants per microgram of DNA using the protoplast-$CaCl_2$ method. The transforming vector pBARGEM 7-1 which had the phosphinothricin resistance gene was detected in the restriction enzyme fragments of chromosomal DNA from a transformant by Southern hybridization.

• The Korean Journal of Mycology
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• v.22 no.1
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• pp.46-49
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• 1994

When Coprinus congregatus was cultivated in low pH YpSs medium(pH 4.2), the culture supernatant turned brown earlier than that of normal(pH 7.1) medium resulted from the melanization synthesized by the secreted laccase reaction. The pH of medium became 5.2 after 24 h incubation. Laccase which was deeply related to the fungal development might also be implicated in the neutralization of excess hydrogen ions to protect from acidification of cytoplasm.

• Journal of Microbiology
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• v.33 no.2
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• pp.146-148
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• 1995

Coprinus congregatus has a membrane-associated laccase which is not secreted into culture media. A mutant monokaryon obtained, by U. V. irradiation followed by protoplast generation and regeneration method, was successfully isolated. When the mutant was grown on a agar plate or in a liquid medium, it secreted laccase while the wild type did not under the same growth conditions. The laccase of the mutant was compared with that of wild type did not under the same growth conditions. The laccase of the mutant was compared with that of wild type of native PAGE analysis, and showed identical mobility.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.206.2-206
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.207.1-207
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.273.1-273
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• 1997

No Abstract, See Full Text