• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1781-1789
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• 2016

Glargine insulin is a long-acting insulin analog that helps blood glucose maintenance in patients with diabetes. We constructed the pPT-GI vector to express prepeptide glargine insulin when transformed into Escherichia coli JM109. The transformed E. coli cells were cultured by fed-batch fermentation. The final dry cell mass was 18 g/l. The prepeptide glargine insulin was 38.52% of the total protein. It was expressed as an inclusion body and then refolded to recover the biological activity. To convert the prepeptide into glargine insulin, citraconylation and trypsin cleavage were performed. Using citraconylation, the yield of enzymatic conversion for glargine insulin increased by 3.2-fold compared with that without citraconylation. After the enzyme reaction, active glargine insulin was purified by two types of chromatography (ion-exchange chromatography and reverse-phase chromatography). We obtained recombinant human glargine insulin at 98.11% purity and verified that it is equal to the standard of human glargine insulin, based on High-performance liquid chromatography analysis and Matrix-assisted laser desorption/ionization Time-of-Flight Mass Spectrometry. We thus established a production process for high-purity recombinant human glargine insulin and a method to block Arg (B31)-insulin formation. This established process for recombinant human glargine insulin may be a model process for the production of other human insulin analogs.

• The Journal of the Acoustical Society of Korea
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• v.15 no.5
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• pp.44-52
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• 1996

Acoustical characteristics of internal pipe structures and a loudspeaker of the thermoacoustic refrigerator are analyzed by using the transfer matrix method. The resonator system is dismantled into verious basic acoustic elements, and then linearized transfer matrices are serially combined with the dynamical system of linearized loudspeaker model, that the total system of thermoacoustic refrigerator can be analyzed in terms of frequency characteristics and acoustic field shape. Additionally, by using equations for energy flow through the capillary stack, the temperature distribution over the stack is numerically estimated. After expressing the acoustic work flow, thermoacoustic flow, and energy loss per unit length in a single capillary duct by using the transverse functional variations, overall energy flow rate and energy balance are obtained for the whole capillary stack. The final expression for energy flow through the stack is numerically evaluated by varying physical parameters obtained from the sound field analysis. After confirming good agreements between predicted and experimental results for the interior sound field of a refrigerator model, the thermoacoustic characteristics of Hofler's apparatus is analyzed by the proposed method and it is observed that the results agree well with Hofler's experimental results.

• JSTS:Journal of Semiconductor Technology and Science
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• v.16 no.6
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• pp.800-807
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• 2016

We propose a fast algorithm using Extreme Contour Point (ECP) to detect the angle of rotated images, is implemented by rotation feature of one covered frame image that can be applied to correct the rotated images like in image processing for real time applications, while CORDIC is inefficient to calculate various points like high definition image since it is only possible to detect rotated angle between one point and the other point. The two advantages of this algorithm, namely compatibility to images in preprocessing by using Sobel edge process for pattern recognition. While the other one is its simplicity for rotated angle detection with cyclic shift of two $1{\times}n$ matrix set without complexity in calculation compared with CORDIC algorithm. In ECP, the edge features of the sample image of gray scale were determined using the Sobel Edge Process. Then, it was subjected to binary code conversion of 0 or 1 with circular boundary to constitute the rotation in invariant conditions. The results were extracted to extreme points of the binary image. Its components expressed not just only the features of angle ${\theta}$ but also the square of radius $r^2$ from the origin of the image. The detected angle of this algorithm is limited only to an angle below 10 degrees but it is appropriate for real time application because it can process a 200 degree with an assumption 20 frames per second. ECP algorithm has an O ($n^2$) in Big O notation that improves the execution time about 7 times the performance if CORDIC algorithm is used.

• Journal of Broadcast Engineering
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• v.25 no.4
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• pp.598-608
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• 2020

In this paper, we propose a color correction technique for images acquired through a multi-view camera system for acquiring a 3D model. It is assumed that the 3D volume is captured indoors, and the position and intensity of the light is constant over time. 8 multi-view cameras are used, and converging toward the center of the space, so even if the lighting is constant, the intensity and angle of light entering each camera may be different. Therefore, a color optimization function is applied to a color correction chart taken from all cameras, and a color conversion matrix defining a relationship between the obtained 8 images is calculated. Using this, the images of all cameras are corrected based on the standard color correction chart. This paper proposed a color correction method to minimize the color difference between cameras when acquiring an image using 8 cameras of 3D objects, and experimentally proved that the color difference between images is reduced when it is restored to a 3D image.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2689-2698
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• 2013

Epithelial-to-mesenchymal transition (EMT) is a collection of events that allows the conversion of adherent epithelial cells, tightly bound to each other within an organized tissue, into independent fibroblastic cells possessing migratory properties and the ability to invade the extracellular matrix. EMT contributes to the complex architecture of the embryo by permitting the progression of embryogenesis from a simple single-cell layer epithelium to a complex three-dimensional organism composed of both epithelial and mesenchymal cells. However, in most tissues EMT is a developmentally restricted process and fully differentiated epithelia typically maintain their epithelial phenotype. Recently, elements of EMT, specially the loss of epithelial markers and the gain of mesenchymal markers, have been observed in pathological states, including epithelial cancers. Increasing evidence has confirmed its presence in human colon during colorectal carcinogenesis. In general, chronic inflammation is considered to be one of the causes of many human cancers including colorectal cancer(CRC). Accordingly, epidemiologic and clinical studies indicate that patients affected by ulcerative colitis and Crohn's disease, the two major forms of inflammatory bowel disease, have an increased risk of developing CRC. A large body of evidence supports roles for the SMAD/STAT3 signaling pathway, the NF-kB pathway, the Ras-mitogenactivated protein kinase/Snail/Slug and microRNAs in the development of colorectal cancers via epithelial-tomesenchymal transition. Thus, EMT appears to be closely involved in the pathogenesis of colorectal cancer, and analysis refered to it can yield novel targets for therapy.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.7
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• pp.881-886
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• 2005

This paper presents intelligent digital redesign method of global approach for hybrid state space fuzzy-model-based controllers. For effectiveness and stabilization of continuous-time uncertain nonlinear systems under discrete-time controller, Takagi-Sugeno(TS) fuzzy model is used to represent tile complex system. And global approach design problems viewed as a convex optimization problem that we minimize the error of the norm bounds between nonlinearly interpolated linear operators to be matched. Also, by using the power series, we analyzed nonlinear system's uncertain parts more precisely. When a sampling period is sufficiently small, the conversion of a continuous-time structured uncertain nonlinear system to an equivalent discrete-time system have proper reason. Sufficiently conditions for the global state-matching of tile digitally controlled system are formulated in terms of linear matrix inequalities (LMIs). Finally, a TS fuzzy model for the chaotic Lorentz system is used as an example to guarantee the stability and effectiveness of the proposed method.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.114.1-114.1
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• 2011

Bioenergy production from lignocellulosic biomass and agriculture wastes have been attracted because of its sustainable and non-edible source. Especially, canola is considered as one of the best feedstock for renewable fuel production. Oil extracted canola and its agriculture residues are reuseable for bioethanol production. However, a pretreatment step is required before enzymatic hydrolysis to disrupt recalcitrant lignocellulosic matrix. To increase the sugar conversion, more efficient pretreatment process was necessary for removal of saccharification barriers such as lignin. Alkaline pretreatment makes the lignocellulose swollen through solvation and induces more porous structure for enzyme access. In our previous work, aqueous ammonia (1~20%) was utilized for alkaline reagent to increase the crystallinity of canola residues pretreatment. In this study, significant factors for efficient soaking in aqueous ammonia pretreatment on canola residues was optimized by using the response surface method (RSM). Based on the fundamental experiments, the real values of factors at the center (0) were determined as follows; $70^{\circ}C$ of temperature, 17.5% of ammonia concentration and 18 h of reaction time in the experiment design using central composition design (CCD). A statistical model predicted that the highest removal yield of lignin was 54% at the following optimized reaction conditions: $72.68^{\circ}C$ of temperature, 18.30% of ammonia concentration and 18.30 h of reaction time. Finally, maximum theoretical yields of soaking in aqueous ammonia pretreatment were 42.23% of glucose and 22.68% of xylose.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.73-73
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• 2011

We will report a few methods to improve the efficiency and stability in small molecule based organic solar cells, including the formation of bulk heterojunctions (BHJs) through alternative thermal deposition (ATD), the use of a micro-cavity structure and interface modifications. By ATD which is a simple modification of conventional thermal evaporation, the thicknesses of alternative donor and acceptor layers were precisely controlled down to 0.1 nm, which is critical to form BHJs. The formation of a BHJ in copper(II) phthalocyanine (CuPc) and fullerene (C60) systems was confirmed by AFM, GISAXS and absorption measurements. From analysis of the data, we found that the CuPc|C60 films fabricated by ATD were composed of the nanometer sized disk shaped CuPc nano grains and aggregated C60, which explains the phase separation of CuPc and C60. On the other hand, the co-deposited CuPc:C60 films did not show the existence of separated CuPc nano grains in the CuPc:C60 matrix. The OPV cells fabricated using the ATD method showed significantly enhanced power conversion efficiency compared to the co-deposited OPV cells under a same composition [1]. We will also present by numerical simulation that adoption of microcavity structure in the planar heterojunction can improve the short circuit current in single and tandem OSCs [2]. Interface modifications also allowed us to achieve high efficiency and high stability OSCs.

• Journal of the Korean Institute of Intelligent Systems
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• v.17 no.3
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• pp.285-290
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• 2007

In this paper, we presents robust digital redesign (DR) method for observer-based linear time-invariant (LTI) system. The term of DR involves converting an analog controller into an equivalent digital one by considering two condition: state-matching and stability. The design problems viewed as a convex optimization problem that we minimize the error of the norm bounds between interpolated linear operators to be matched. Also, by using the bilinear and inverse bilinear approximation method, we analyzed the uncertain parts of given observer-based system more precisely, When a sampling period is sufficiently small, the conversion of a analog structured uncertain system to an equivalent discrete-time system have proper reason. Sufficiently conditions for the state-matching of the digitally controlled system are formulated in terms of linear matrix inequalities (LMIs).

• The Transactions of the Korea Information Processing Society
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• v.1 no.4
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• pp.469-478
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• 1994

In this paper, a high speed, high resolution information processing digital- analog converter was designed for high definition color graphic, digital image signal processing, HDTV. For high speed operation, matrix type current cell array, latch which is not use pipelined, and two dimensional structure decoder using transmission gate were designed. It is adopted to fast-conversion, low-power implementation and exhibited high performance at linearity and accuracy. To reduce silicon area and to maintain resolution, current cell array composed of weighted and non-weighted current cells. In this paper, deglitching current cell design for high accuracy, new switching algorithm assert to reduce switching error. It's This circuit dissipates 130W with a 5-V power supply, and operate above 100MHz with 10 bit resolution.