• Journal of Acupuncture Research
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• 제21권3호
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• pp.107-119
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• 2004

Objective : The purpose of this study was to investigate the effect of Bee Venom and melittin on the lipopolysaccharide(LPS) and sodium nitroprusside(SNP)-induced expressions of Cell viability, nitric oxide(NO), inducible nitric oxide synthase(iNOS), extra-signal response kinase(ERK), jun N-terminal Kinase(JNK) and p38 kinase(p38)- mitogen activated protein kinase(MAPK) Family- in RAW 264.7 cells, a murine macrophage cell line. Methods : The expressions of cell viability by MTT assay, NO by Nitrite assay and iNOS, ERK, JNK and p38 were determined by Western blotting. Results : 1. Compared with the control group, 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin increased cell viability of RAW 264.7 induced by LPS and SNP significantly respectively. 2. Compared with the control group, 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of NO induced by LPS and SNP significantly respectively. 3. Compared with the control group, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of iNOS induced by LPS significantly and 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of iNOS induced by SNP significantly. 4. Compared with the control group, the expression of ERK induced by LPS and SNP decreased significantly in the treatment groups of $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin, which of p-ERK by LPS also did in 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin, but which of p-ERK by SNP did not decrease. 5. Compared with the control group, the. expression of JNK induced by LPS and SNP decreased significantly in the treatment groups of 5, $10{\mu}g/m{\ell}$ melittin, which of p-JNK by LPS in 5, $10{\mu}g/m{\ell}$ melittin and by SNP in $1{\mu}g/m{\ell}$ bee venom and $10{\mu}g/m{\ell}$ melittin decreased significantly. 6. Compared with the control group, the expression of p38 induced by LPS did not have significant difference, which induced by SNP decreased significantly in the treatment groups of 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin. p-p38 induced by LPS decreased significantly in the treatment group of $10{\mu}g/m{\ell}$ of melittin, which induced by SNP also decreased significantly in 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin.

• 한국산학기술학회논문지
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• 제14권12호
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• pp.6427-6433
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• 2013

열차를 안전하면서도 신속하게 운행하기 위해서는 항상 선행열차와 후속열차가 일정한 간격을 유지하면서 운행하여야 한다. 이를 위해 일정한 간격의 폐색구간을 설정하고, 열차의 안전운전을 위해 단일 폐색구간에는 1대의 열차만이 운행될 수 있도록 제어하는 폐색시스템이 필수적으로 요구된다. 기존의 폐색시스템은 신호기에 신호를 현시하는 계전기 방식의 자동폐색장치(ABS: Automatic Block System)로 구성되며, 이것은 연동장치를 통해 궤도회로 등선로변 장치들과 연동된다. 연동장치의 경우 국산 전자연동장치로 교체되고 있지만, 폐색장치는 여전히 아날로그 방식의 계전기 형태를 사용하고 있으며, 다른 선로변 장치들과 독립적으로 설치된다. 따라서 기존의 폐색장치는 건설과 유지보수 측면에서 많은 문제점을 가지고 있다. 또한 현재 국내 기존선에 사용되고 있는 ABS와 선로변 전자장치(LEU: Lineside Electronic Unit)는 동일한 장소에서 동일한 신호 정보에 의해 열차를 제어하고 있음에도 불구하고 별개 시스템으로 분리되어 설치되기 때문에, 각 제품의 단가 및 유지보수 비용 측면에서 효율적이지 않다. 따라서 본 논문에서는 기존 폐색장치인 ABS에 통신 및 디지털 전자 기술을 접목시킨 통합형 전자폐색제어장치(integrated electronic block system)와 그것의 현장시험 결과를 소개한다. 현장시험은 1월초부터 8월말까지 8개월간 총 8번의 주기점검을 통해 시행하였으며, 이를 통해 개발된 전자폐색장치의 유효성과 적합성을 확인하였다.

• 한국전자통신학회논문지
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• 제14권2호
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• pp.309-316
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• 2019

본 논문은 로컬 클록 왜곡을 보상하는 낮은 지터 성능의 지연 고정 루프를 제시한다. 제안된 DLL은 위상 스플리터, 위상 검출기(PD), 차지 펌프, 바이어스 생성기, 전압 제어 지연 라인(Voltage Controlled Delay Line) 및 레벨 변환기로 구성된다. VCDL(: Voltage Controlled Delay Line)은 CML(: Current Mode Logic)을 사용하는 자체 바이어스 지연 셀을 사용하여 온도에 민감하지 않고 잡음을 공급한다. 위상 스플리터는 VCDL의 차동 입력으로 사용되는 두 개의 기준 클록을 생성한다. 제안된 회로의 PD는 CML에 비해 적은 전력을 소비하는 CMOS 로직을 사용하기 때문에 PD는 위상 스플리터의 유일한 단일 클록을 사용한다. 따라서 VCDL의 출력은 로컬 클록 분배 회로뿐만 아니라 PD에 사용되므로 레벨 변환기에 의해 레일-투-레일 신호로 변환된다. 제안된 회로는 $0.13{\mu}m\;CMOS$ 공정으로 설계되었으며, 주파수가 1GHz인 클록이 외부에서 인가된다. 약 19 사이클 후에 제안된 DLL은 잠금이 되며, 클록의 지터는 1.05ps이다.

• 소음진동
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• 제10권6호
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• pp.1059-1066
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• 2000

본 논문에서는 고조파 소음을 내는 시스템의 기본 주파수 추정을 통한 새로운 피드백 능동소음제어 방법을 제안한다. 기준신호를 측정하기 어려운 상황에서 종래의 다중 정현파 소음의 능동제어 방법은 기준신호를 측정하는 대신 추정된 주파수를 이용하여 기준신호를 만들어낸다.$^{(4)}$ 그러나 맥놀이현상(beating phenomenon)이 존재할 때는 순간적으로 특정 주파수 성분이 사라지게 되므로 적응 주파수 추정이 어렵게 되어 맥놀이현상에 의한 주파수 추정에서의 혼돈은 만들어진 기준신호를 쓸모없게 만든다. 제안된 알고리즘은 두 가지 부분으로 구성된다. 첫째는 기본주파수 추정을 이용한 기준신호 발생기이고 둘째는 기존의 피드포워드 제어 부분이다. 제안된 결정 규칙을 이용한 기본주파수 추정알고리즘은 맥놀이현상에 둔감할 뿐 아니라 기존의 직렬형 적응노치필터 방법에 비해 좋은 추종성능과 적은 주파수 추정 오차의 분산을 갖는다.$^{(4)}$ 더욱이 신호대잡음비가 좋지 않아 기존의 주파수 추정방법으로는 추정될 수 없는 주파수 성분가지 제어가 가능하다. 제안된 능동소음제어 방법의 성능을 검증하기 위해서 실측한 선박의 객실 소음과 자동차의 시변하는 엔진부밍소음에 대해서 모의 실험을 수행한다.

• 한국정보통신학회논문지
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• 제4권1호
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• pp.77-88
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• 2000

본 논문에서는 INMARSAT-B형 송신기에 사용되는 L-BAND(1626.5-1646.5 MHz)용 3단 가변이득 전력증폭기를 연구 개발하였다. 3단 가변이득 전력증폭기는 구동증폭단과 전력증폭단에 의해 고출력 모드일 때 +42 dBm, 중간출력 모드일 때는 +38 dBm, 저출력 모드일 때는 +34 dBm의 전력으로 증폭되며, 각각에 대해 상한 +1 dBm과 하한 2 dBm의 오차를 허용한다. 제작의 간편성 때문에 전체 3단 가변이득 전력증폭기를 크게 구동증폭단과 전력증폭단 두 부분으로 나누어 구현하였으며, 전력증폭부를 구동하기 위한 구동단은 HP사의 MGA-64135와 Motorola사의 MRF-6401을 사용하였으며, 전력증폭단은 ERICSSON사의 PTE-10114와 PTF-10021을 사용하여 RP부, 온도보상회로, 출력 조절회로 및 출력 검출회로를 함께 집적화 하였다. 이득조절은 디지털 감쇠기를 사용하였으며, 출력신호의 세기를 검출하기 위하여 20 dB 방향성 결합기를 이용하였다. 제작된 3단 가변이득 전력증폭기는 20 MHz대역폭 내에서 소신호 이득이 41.6 dB, 37.6 dB, 33.2 dB 를 얻었으며, 입출력 정재파비는 1.3:1 이하, 12 dBm의 PldB, PldB 출력레벨에서 3 dB Back off 시켰을 때 36.5 dBc의 IM3를 얻었다. 1636.5 MHz 주파수에 대해 출력전력은 43 dBm으로서 설계시 목표로 했던 최대 출력전력 20 Watt를 얻었다.

• BMB Reports
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• 제33권2호
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• Tribology and Lubricants
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• 제30권3호
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• pp.139-145
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• 2014

Nowadays, most micro-patterns are manufactured during flow line production. However, a conventional rotary chemical mechanical polishing (CMP) system has a limited throughput for the fabrication of large and flexible electronics. To overcome this problem, we propose a novel linear roll-CMP system for the planarization of large-area electronics. In this paper, we present a statistical analysis on the linear roll-CMP process of copper-clad laminate (CCL) to determine the impacts of process parameters on the material removal rate (MRR) and its non-uniformity (NU). In the linear roll-CMP process, process parameters such as the slurry flow rate, roll speed, table feed rate, and down force affect the MRR and NU. To determine the polishing characteristics of roll-CMP, we use Taguchi's orthogonal array L16 (44) for the experimental design and F-values obtained by the analysis of variance (ANOVA). We investigate the signal-to-noise (S/N) ratio to identify the prominent control parameters. The "higher is better" for the MRR and "lower is better" for the NU were selected for obtaining optimum CMP performance characteristics. The experimental and statistical results indicate that the down force and roll speed mainly affect the MRR and the down force and table feed rate determine the NU in the linear roll-CMP process. However, over 186.3 N of down force deteriorates the NU because of the bending of substrate. Roll speed has little relationship to the NU and the table feed rate does not impact on the MRR. This study provides information on the design parameter of roll-CMP machine and process optimization.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1078-1083
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• 2006

Capsaicin is the active ingredient in chili pepper and has an inhibitory effect on Helicobacter pylori growth and $NF-{\kappa}B$ activation. The present study examined the effect of capsaicin on interleukin (IL)-8 production by H. pylori ATCC 43504-infected MKN-45 cells, a gastric epithelial cell line. The viability of the MKN-45 cells treated with capsaicin at 0, 50, 100, 250, and $500\;{\mu}M$ was 99, 98, 99, 99, and 85%, respectively. A capsaicin concentration as low as $50\;{\mu}M$ significantly inhibited the IL-8 production induced by H. pylori ATCC 43504 infection (43.2% of control) during 24 h of incubation. However, low concentrations of capsaicin $(50\;and\;100{\mu}M)$ did not significantly inhibit the IL-8 production by $TNF-{\alpha}-$ or PMA-treated MKN-45 cells. Therefore, the overall inhibitory effect of capsaicin on H. pylori ATCC 43504 was the sum of H. pylori ATCC 43504 growth inhibition, host cell survival, and $NF-{\kappa}B$ signal cascade inhibition.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.1077-1082
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• 2015

Recent studies have suggested that the RAS protein activator like-1 (RASAL1) functions as a tumor suppressor in vitro and may play an important role in the development of gastric cancer. However, whether or not RASAL1 suppresses tumor growth in vivo remains to be determined. In the present study, we investigated the role of RASAL1 in gastric carcinogenesis using an in vivo xenograft model. A lentiviral RASAL1 expression vector was constructed and utilized to transfect the human poorly differentiated gastric adenocarcinoma cell line, BGC-823. RASAL1 expression levels were verified by quantitative real-time RT-PCR and Western blotting analysis. Then, we established the nude mice xenograft model using BGC-823 cells either over-expressing RASAL1 or normal. After three weeks, the results showed that the over-expression of RASAL1 led to a significant reduction in both tumor volume and weight compared with the other two control groups. Furthermore, in xenograft tissues the increased expression of RASAL1 in BGC-823 cells caused decreased expression of p-ERK1/2, a downstream moleculein the RAS/RAF/MEK/ERK signal pathway. These findings demonstrated that the over-expression of RASAL1 could inhibit the growth of gastric cancer by inactivation of the RAS/RAF/MEK/ERK pathway in vivo. This study indicates that RASAL1 may attenuate gastric carcinogenesis.

• 대한의생명과학회지
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• 제7권4호
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• pp.167-172
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• 2001

Nebulin is an approximately 700 kDa filamentous protein in vertebrate skeletal muscle. It binds to the Z line and also binds side-by-side to the entire thin actin filament in a sarcomere. The correlation of nebulin size with thin filament length have led to the suggestion that nebulin acts as a molecular ruler for the length of thin filaments. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain. SH3 domains have been identified in an ever-increasing number of proteins important for a wide range of cellular processes, from signal transduction to cytoskeleton assembly and membrane localization. However, the exact physiological role of SH3 domains remains, in many cases, unclear. To explore the role of nebulin SH3 in the cytoskeletal rearrangement that accompanies myoblast differentiation, we transfected sense and antisense nebulin SH3 domain fused to enhanced green fluorescent protein in myoblast. Cells expressing nebulin SH3 fragment showed decrease of cell-cell adhesion, and cells transfected with antisense nebulin SH3 gene showed a rounded cell morphology and loss of cell-matrix adhesion. No alteration in cell shape and differentiation were observed in control cells expressing enhanced green fluorescent protein. Perturbation of nebulin altered the cell shape and disrupted cell adhesion in myoblast, demonstrating that nebulin can affect cytoskeleton rearrangement.