• Journal of Microbiology
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• v.44 no.4
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• pp.375-382
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• 2006

From its initial colonization to causation of disease, Streptococcus pneumoniae has evolved strategies to cope with a number of stressful in vivo environmental conditions. In order to analyze a global view of this organism's response to heat shock, we established a 2-D electrophoresis proteome map of the S. pneumoniae D39 soluble proteins under in vitro culture conditions and performed the comparative proteome analysis to a 37 to $42^{\circ}C$ temperature up-shift in S. pneumoniae. When the temperature of an exponentially growing S. pneumoniae D39 culture was raised to $42^{\circ}C$, the expression level of 25 proteins showed changes when compared to the control. Among these 25 proteins, 12 were identified by MALDI-TOF and LC-coupled ESI MS/MS. The identified proteins were shown to be involved in the general stress response, energy metabolism, nucleotide biosynthesis pathways, and purine metabolism. These results provide clues for understanding the mechanism of adaptation to heat shock by S. pneumoniae and may facilitate the assessment of a possible role for these proteins in the physiology and pathogenesis of this pathogen.

• Food Science of Animal Resources
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• v.25 no.3
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• pp.350-356
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• 2005

We examined the enhancement of thermotolerance for storage conferred on Lactobacillus acidophilus 30SC by adaptation to different stresses. The viable cells of Lactobacillus acidophilus 30SC were compared with their viability prior to heating at $45,\;55^{\circ}C\;and\;60^{\circ}C$. Heat-adapted ($45^{\circ}C$ for 15 min) L. acidophilus 30SC in MRS broth exhibited higher survivability at lethal temperature of $55^{\circ}C$ than control. Cellular protein profiles of L. acidophilus 30SC during heat adaptation were examined with SDS-PAGE, and scanning electron microscopy. When L. acidophilus 30SC was heat-adapted at $55^{\circ}C$ for 15min, 5 new protein spots of ca $8\~45\;kDa$ size were observed on 2D SDS-PAGE. It was presumed that new proteins of L. acidophilus 30SC were produced to adapt to the environment of higher growth temperature.

• Journal of the Korea Convergence Society
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• v.12 no.11
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• pp.135-142
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• 2021

The purpose of this study was to determine the effect of extracorporeal shock wave therapy for the neck-shouler muscles of complex pain scales of the 20s adults with VDT Syndrome to prolonged smartphone use. Twenty subjects with neck pain due to VDT syndrome participated in the experiment. They were randomly divided into 10 experimental groups and 10 control groups. Experimental subjects were conducted for 4 weeks, 5 days a week. The experimental group was subjected to extracorporeal shock wave therapy 2000 times at an intensity of 5 Hz and 2.0 bar once, and the control group performed self-stretching exercise for 30 minutes once. Subjects measured and compared neck-shoulder pain with VAS and PPT before and after the experiment. Data analysis was performed with SPSS win 18.0, and correspondence comparison was performed to analyze the results of the experimental group and the control group before and after the experiment. After the experiment, the experimental group decreased more in VAS and PPT of the neck-shoulder muscle. but the control group was no difference. Though these results, extracorporeal shock wave therapy was showed a reduction in pain after treatment than before treatment but there are no difference in the pain reduction after self-stretching exercise. Thus, extracorporeal shock wave therapy is expected to be used as a variety of treatments to reduce neck-shoulder pain as a cause of VDT syndrome.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.9
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• pp.1095-1104
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• 1997

When a high-speed railway train enters a tunnel, a compression wave is generated ahead of the train and propagates along the tunnel, compressing and accelerating the rest air in front of the wave. At the exit of the tunnel, an impulsive wave is emitted outward toward the surrounding, which causes a positive impulsive noise like a kind of sonic boom produced by a supersonic aircraft. With the advent of high-speed train, such an impulsive noise can be large enough to cause the noise problem, unless some attempts are made to alleviate its pressure levels. For the purpose of the impulsive noise reduction, the present study investigated the effect of a vertical bleed duct on the compression wave propagating into a model tunnel. Numerical results were obtained using a Piecewise Linear Method and testified by experiment of shock tube with an open end. The results showed that the vertical bleed duct reduces the maximum pressure gradient of compression wave front by about 30 percent, compared with the straight tunnel without the bleed duct. As the width of the vertical bleed duct becomes larger, reduction of the impulsive noise is expected to be greater. However the impulsive noise is independent of the height of the vertical bleed duct.

• Animal Bioscience
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• v.34 no.4
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• pp.724-733
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• 2021

Objective: The objectives of this study were to investigate the direct antioxidative effect of 90 Kda heat shock protein (Hsp90) obtained from duck muscle. Methods: The interaction of Hsp90 with phospholipids and oxidized phospholipids was studied with surface plasmon resonance (SPR), and their further oxidation in the presence of Hsp90 was evaluated with thiobarbituric acid reactive substances (TBARS) assay. The scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) was measured, and the electron paramagnetic resonance (EPR) spectroscopy in combination with 5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide and 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) was utilized to determine the abilities of Hsp90 in scavenging hydroxyl and PTIO radicals. Results: SPR showed Hsp90 could bind with both phospholipids and oxidized phospholipids, and prevent their further oxidation by the TBARS assay. The DPPH and ABTS scavenging activity increased with Hsp90 concentration, and could reach 27% and 20% respectively at the protein concentration of 50 μM. The EPR spectra demonstrated Hsp90 could directly scavenge ·OH and PTIO· radicals. Conclusion: This suggests that Hsp90, a natural antioxidant in meat, may play an important role in cellular defense against oxidative stress, and may have potential use in meat products.

• The Journal of the Korea institute of electronic communication sciences
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• v.19 no.4
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• pp.647-654
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• 2024

In military weapon systems, control sticks for weapon operation are essential. This paper presents the design and performance evaluation of a control stick for weapon systems using RS-422 communication and an embedded processor. This control stick is ergonomically designed, considering the hand dimensions of Korean pilots and mission convenience. The circuitry and software based on RS-422 communication were designed using an embedded processor. A prototype of the control stick was manufactured and subjected to a series of tests, including basic performance tests and various environmental tests such as altitude, high temperature, low temperature, temperature shock, humidity, rain, vibration shock, and acceleration, all of which met the required standards.

• 연구논문집
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• s.28
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• pp.73-87
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• 1998

A pneumatic control system of compressed air as a working fluid has a variety of advantages such as low price, high respondence, non-explosion and good control performance and thus has many applications in the field of automobile, electronic and semiconductor industry. However, it has a difficulty in contolling a precise position due to quick response of system and compressibility of working fluid and. in particular, shock stress may occur due to an external load, resulting in fracture of a cylinder cap unless cushion device is equipped in the linear actuator. To avoid this, a cushion device should be installed for damping effect of the external load and the supply pressure as well as for decreasing shock stress and vibration caused by high speed rotation. Previous studies include dimensionless analyses and computer simulations of cushion capability and experiments of horizontally-mounted cylinder performances. A new attempt is experimentally made in this study using a vertically-mounted cylinder under an operation condition of 4, 5 and 6 (bar) as supply pressure and 40, 70 and 100 (kgf) as external load. It turns out that the cushion pressure is mainly a function of the external load rather than the supply pressure. The cushion characteristics was also revealed in the meter-in circuit.

• Journal of Microbiology
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• v.34 no.2
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• pp.158-165
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• 1996

The signal transduction pathways through the RAS gene product and adenyl cyclease play a critical role in regulation of the cell cycle in yeast, Saccharomyces cerevisiae. We examined the genetic relationship between the spt3 gene and ras/cAMP pathway. A mutation in the SPT3 gene suppressed cell cycle arrest at the G1 phase caused by either an inactivation of the RAS or CYR1 gene which encodes a yeast homologue of human ras proto-oncogene or adenyl cyclase, respectively. The phenotypes such as sporulation and heat shock resistancy, that resulted from a partial inactivation of the RAS or CYR1 genes, were also suppressed by the spt3 mutation. Expression of the SSA1 gene encoding one of th heat shock proteins (Hsp70) can be induced by heat shock or nitrogen starvation. Expression of this gene is derepressed in cry1-2 and spt3 mutants. The bcy 1 mutation repressed by the bcy1 mutation, but not in spt3 mutants. These results suggest that the SPT gene is involved in expression of genes that are affected by the RAS/cAMP pathway.

• Korean Journal of Environmental Biology
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• v.21 no.3
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• pp.313-318
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• 2003

Environmental stresses, such as heat shock, alcohol and physiological salt have been shown to induce a group of protein called heat shock protein (HSPs) in various tissues. In this investigation, we studied that arsenic stress would alter contraction of isolated rat aorta and expression of heat shock protein 70 and investigated the relation between expression of HSP 70 and vascular contractility of isolated rat aorta. Rat aorta strips, mounted in organ baths were exposed to 0, 0.5, 1,2 and 4 mM arsonic for 60 min. and 1,3 and 8 hours later tested for contractile response and expression of heat shock protein 70. Contractility of rat aorta were determined by isometric transducer connected to computerized physiograph and expression of HSP 70 was characterized by western blotting, respectively. Potassium chloride (55 mM) significantly augmented vascular contractility of yat aorta by 39％ compared with the control at 8 hours but not one or three hours after treatment of 4 mM arsenic. Arsonic stress (4 mM) also increased the expression of HSP 70 in rat aorta at 8 hours but one or three hours compared with the control and HSP expressed in vascular smooth muscle cells and some expressed in endothelium cells. These results suggest that arsenic stress not only did alter the magnitude of the contractile response to high potassium chloride but also increased the expression of HSP 70 in the rat aorta.

• Animal cells and systems
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• v.14 no.4
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• pp.267-274
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• 2010

The decrease in sperm quality under heat stress causes a great loss in animal husbandry production. In order to reveal the mechanism underlying the sperm quality decrease caused by heat stress, we first established a mild heat-treated mouse model. Then, the sperm quality was identified. Further, the testicular proteome profile was mapped and compared with the control using 2D electrophoresis and mass spectrometry. Finally, the differential expressed proteins involved in the heat stress response were identified by real-time PCR and Western blotting. The results showed that heat stress caused a significant reduction in mouse sperm quality (P<0.05). Further, 52 protein spots on the 2D gel were found to differ between the heat-shocked tissues and the control. Of these spots, some repair proteins which might provide some explanation for the influence on sperm quality were found. We then focused on Bag-1, Hsp40, Hsp60 and Hsp70, which were found to be differently expressed after heat shock (P<0.05). Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorders.