Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Effects of a mild heat treatment on mouse testicular gene expression and sperm quality

  • Zhao, Jun (School of Life Sciences, Northeast Normal University, Jilin Key Laboratory of Animal Resource Conservation and Utilization) ;
  • Zhang, Ying (Veterinary College, Jilin University) ;
  • Hao, Linlin (Veterinary College, Jilin University) ;
  • Wang, Jia (Veterinary College, Jilin University) ;
  • Zhang, Jiabao (Experiment Animal Centre, Jilin University) ;
  • Liu, Songcai (Veterinary College, Jilin University) ;
  • Ren, Bingzhong (School of Life Sciences, Northeast Normal University, Jilin Key Laboratory of Animal Resource Conservation and Utilization)
  • Received : 2010.06.18
  • Accepted : 2010.08.09
  • Published : 2010.12.31
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Abstract

The decrease in sperm quality under heat stress causes a great loss in animal husbandry production. In order to reveal the mechanism underlying the sperm quality decrease caused by heat stress, we first established a mild heat-treated mouse model. Then, the sperm quality was identified. Further, the testicular proteome profile was mapped and compared with the control using 2D electrophoresis and mass spectrometry. Finally, the differential expressed proteins involved in the heat stress response were identified by real-time PCR and Western blotting. The results showed that heat stress caused a significant reduction in mouse sperm quality (P<0.05). Further, 52 protein spots on the 2D gel were found to differ between the heat-shocked tissues and the control. Of these spots, some repair proteins which might provide some explanation for the influence on sperm quality were found. We then focused on Bag-1, Hsp40, Hsp60 and Hsp70, which were found to be differently expressed after heat shock (P<0.05). Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorders.

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References

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