• Biotechnology and Bioprocess Engineering:BBE
• /
• 제8권2호
• /
• pp.59-63
• /
• 2003

The protein disulfide isomerase (PDI) reaction kinetics has been studied to evaluate its effect on the monoclonal antibody (Mab) refolding and assembly which accompanies disulfide bend formation. The MAb in vitro assembly experiments showed that the assembly rate of heavy and light chains can be greatly enhanced in the presence of PDI as compared to the rate of assembly obtained by the air-oxidation. The reassembly patterns of MAb in-termediates were identical for both with and without PDI, suggesting that the PDI does not determine the MAb assembly pathway, but rather facilitates the rate of MAb assembly by promoting PDI catalyzed disulfide bond formation. The effect of growth rate on PDI activities for MAb production has also been examined by using continuous culture system. The specific MAb productivity of hybridoma cells decreased as the growth rate increased. However, PDI activities were nearly constant fur a wide range of growth rates except very high growth rate, indicating that no direct correlation between PDI activity and specific MAb productivity exists.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제1권1호
• /
• pp.13-17
• /
• 1996

The protein disulfide isomerase(PDI) reaction kinetics has been studied to evaluate its effect on the monoclonal antibody(MAb) refolding and assembly which accompanies disulfide bond formation The MAb in vitro assembly experiments showed that the assembly rate of heavy and light chains can be greatly enhanced in the presence of PDI as compared to the rate of assembly obtained by the air-oxidation. The reassembly patterns of MAb intermediates were identical for both with and without PDI, suggesting that the PDI does not determine the MAb assembly pathway, but rather facilitates the rate of MAb assembly by promoting PDI catalyzed disulfide bond formation. The effect of growth rate on PDI activities for MAb production has also been examined by using continuous culture system. The specific MAb productivity of hybridoma cells decreased as the growth rate increased. However, PDI activities were nearly constant for a wide range of growth rates except very high growth rate, indicating that no direct correlation between PDI activity and specific MAb productivity exists.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 1996년도 추계학술대회 논문집
• /
• pp.560-564
• /
• 1996

In recently, annealing process of cold rolled sheet tend to change to continuous annealing process for improving qualify, saving yield. In the meantime as demand for various kind and small lot of products has been increasing, batch annealing has been appreciated for its small restriction for the operation. So, we tested on the effect for a hydrogen contents of atmospheric gas at annealing furnace. As a result of several investigation. We confirmed for the following characteristics ; improved productivity, uniform heating, improved surface quality, saving energy. Therefore, the use of hydrogen instead of nitrogen as the protective gas, combined with high convection in batch annealing furnaces, has shown that considerable increases in furnace output and material quality are attainable. Owing to the low density, high diffusion and reducing character of hydrogen, a better transfer resulting in uniform material temperatures and improved coil surfaces can be achieved.

• 디지털융복합연구
• /
• 제3권2호
• /
• pp.39-57
• /
• 2005

This research is about continuous improvement in productivity for the central and local government administration. It is a comprehensive execution manual that summarizes from preparation to completion of this task. If you follow the process described in this research, you will achieve the following. l. You will motivate the innovators. 2. You will spare yourself of many trial and errors and risks thus increasing the effectiveness, 3. You will eliminate the obstacles to innovation. 4. You will continue to innovate and will be able to assess the results through visible index.

• 한국디지털정책학회:학술대회논문집
• /
• 한국디지털정책학회 2005년도 추계학술대회
• /
• pp.63-81
• /
• 2005

This research is about continuous improvement in productivity for the central and local government administration. It is a comprehensive execution manual that summarizes from preparation to completion of this task. If you follow the process described in this research, you will achieve the following. 1. You will motivate the innovators. 2. You will spare yourself of many trial and errors and risks thus increasing the effectiveness. 3. You will eliminate the obstacles to innovation. 4. You will continue to innovate and will be able to assess the results through visible index.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.245-248
• /
• 2003

As a result of broth substitutions when each culture-mediums were difference, whole culture-medium was found to be best feeding solution for production of PS-7 by B. indica. Maximal production of PS-7 was 1$10.0\;g/{\ell}$ and its conversion rate from 2% (w/v) glucose to PS-7 was 50%. After 48 hr, 50%(v/v) medium of working volume began to substitute in 7L jar fermenter. Production of PS-7 increased after 48hr, recovered productivity of PS-7. Following this preliminary culture, the resultant culture was subjected to continuous flow conditions controlled that the dilution rate were $0.01\;{\sim}\;0.04\;h^{-1}$. Production of PS-7 increased at dilution rate $0.0100\;h^{-1}$ whereas productivity of PS-7 decreased gradually in dilution rate $0.0200\;{\sim}\;0.0400\;h^{-1}$. Maximal production of PS-7 was $10.0\;g/{\ell}$ in continuous culture.

• KSBB Journal
• /
• 제9권1호
• /
• pp.85-90
• /
• 1994

Gellan형 미생물 다당류를 생산하는데 관련된 기초 자료를 얻기 위하여 배지조성, 배양온도 및 초기 pH등에 대한 플라스크 실험을 수행하였으며 이를 근거로 회분식, 유가식 및 연속배양을 실시하였다. 또한 배양액의 비뉴우튼성 유체 특성을 조사하였고 이때의 교반동력을 측정하였다. 플라스크 배양을 통하여 얻은 결과를 이용하여 회분식 배양의 최적조건을 확립하였으며 각종 metabolic parameter들을 추정하였다. 유가식과 연속식 배양을 통하여 생산성 향상을 시도한 결과 유가식과 연속식 배양에서 생산성 향상을 꾀할 수 있었다. 고점성 배양액에서의 물질전달 장해현상을 극복하기 위한 일련의 연구가 필요하다고 사료되며 이로부터 생산성 향상을 크게 기대할 수 있을 것으로 보인다.

• 한국미생물·생명공학회지
• /
• 제23권3호
• /
• pp.346-351
• /
• 1995

To produce ethanol from Jerusalem artichoke powder efficiently, Kluyveromyces marxianus F043 cells were encapsulated in 2% sodium alginate and were cultured in a countinuous reactor to investigate the fermentation properties. Immobilized K. marxianus F043 cells were activated for 48 hours in a fermentor for continuous ethanol production. The culture in a CSTR using a Jerusalem artichoke substrate treated with 2% cellulase showed a decrease in ethanol concentration and an increase in residual saccharide concentration with a increasing dilution rate. Optimum conditions for high ethanol productivity and low residual saccharide output were clarified to be given at a dilution rate of 0.2 h$^{-1}$ and a Jerusalem artichoke medium concentration of 75 g/l. Ethanol productivity of 3.1 g/l-h and saccharide utilization of 62.6% were obtained under the optimum condition. When the fermentation was performed for 3 weeks under these conditions, the effluent medium showed stable ethanol concentrations of 16.3 - 17.9 g/l and viable cells of 6.60-7.16 log cells/ml without contamination. Trace amounts of methyl, n-propyl, iso-butyl, isoamyl alcohols besides ethanol were detected.

• 산업경영시스템학회지
• /
• 제36권4호
• /
• pp.9-17
• /
• 2013

The purpose of this study is to figure out the impacts of technological innovation and cost management on productivity in small shipping industries to come up with developmental implications. To achieve this, a survey was carried out to 150 workers in small shipbuilding industries through April 2 to April 20, 2012. As for findings stated above, technological innovation and cost management in the Korean small shipbuilding industries were key factors that elevate financial and non-financial productivity. In the light of low technological prowess and cost structure of small shipbuilding industries, their productivity can be improved when intensive cost management with production factor technology as know-how is realized through quality management, which product development technology is the top priority as an independent niche strategy. Consequently, the combination of infrastructures in small shipbuilding industries with continuous efforts for cost reduction by the link to the systematized structure can't only secure their independent competitiveness, but raise their productivity.

• Korean Chemical Engineering Research
• /
• 제56권1호
• /
• pp.73-78
• /
• 2018

본 연구는 혼합영양 배양 조건에서 acetate의 주입이 Anabena azollae의 성장에 미치는 영향을 파악하기 위해 수행되었다. 4가지 종류의 acetate 중 ethyle acetate가 Anabena azollae의 성장에 가장 효과적이라고 밝혀졌으며, 주입한 ethyle acetate의 농도가 증가할수록 성장속도는 증가하는 것을 확인하였다. 40 mM의 ethyl acetate의 경우 비성장속도는 $0.979day^{-1}$, 최대바이오매스 생산성은 $0.293g\;L^{-1}\;d^{-1}$로 본 연구에서 배양속도가 가장 빠른 것으로 판명되었다. Acetic acid와 butyl acetate의 경우 Anabena azollae 성장을 방해하는 것으로 나타났다. Aetration의 경우 0.54 vvm에서 성장속도가 가장 빨랐다. 반연속배양에서는 aeration 실험이 끝난 후 연속하여 ethyle acetate 주입을 하여 배양을 하였다. 회분식실험에 비해 반연속배양에서의 비성장속도와 최대바이오매스 생산성은 모두 감소하였지만 최대 농도는 5.91 g/L로 가장 큰 값을 나타내었다.