[KSCI] Korea Science Citation Index Service

Monoclonal Antibody Refolding and Assembly: Protein Disulfide Isomerase Reaction Kinetics

Park, Sun-Ho (Department of Chemical Engineering, Keimyung University)
Ryu, Dewey D.Y. (Department of Chemical Engineering, University of California)

Publication Information

Biotechnology and Bioprocess Engineering:BBE / v.8, no.2, 2003 , pp. 59-63 More about this Journal

Abstract

The protein disulfide isomerase (PDI) reaction kinetics has been studied to evaluate its effect on the monoclonal antibody (Mab) refolding and assembly which accompanies disulfide bend formation. The MAb in vitro assembly experiments showed that the assembly rate of heavy and light chains can be greatly enhanced in the presence of PDI as compared to the rate of assembly obtained by the air-oxidation. The reassembly patterns of MAb in-termediates were identical for both with and without PDI, suggesting that the PDI does not determine the MAb assembly pathway, but rather facilitates the rate of MAb assembly by promoting PDI catalyzed disulfide bond formation. The effect of growth rate on PDI activities for MAb production has also been examined by using continuous culture system. The specific MAb productivity of hybridoma cells decreased as the growth rate increased. However, PDI activities were nearly constant fur a wide range of growth rates except very high growth rate, indicating that no direct correlation between PDI activity and specific MAb productivity exists.

Keywords

monoclonal antibody; protein folding and assembly; protein disulfide isomerase; continuous culture; hybridoma;

Citations & Related Records

Times Cited By Web Of Science : 0 (Related Records In Web of Science)

Reference

1	Formation and isomerization of disulfide bonds in proteins: Protein disulfide-isomerase / [ Hillson,D.A.;Lambert,N.;Freedman,R.B. ] / Methods in Enzymology DOI
2	Effect of protein and peptide inhibitors on the activity of protein disulphide isomerse / [ Morjana,N.A.;Gilbert,H.F. ] / Biochemistry DOI ScienceOn
3	A kinetic analyis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate, and pH / [ Miller,W.M.;Blanch,H.W.;Wilke,C.R. ] / Biotechnol. Bioeng.
4	The thiol-protein disulfide oxidoreductase in human mononuclear cells of blood and bone barrow / [ Ansorge,S.;Mansfeld,H.W.;Held,C.;Broodtaerts,L.;Van Camp,B. ] / Acta Histochem. DOI ScienceOn
5	Role of disulfide interchange enzyme in immunoglobulin synthesis / [ Roth,R.A.;Koshland,M.E. ] / Biochemistry DOI ScienceOn
6	Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein / [ Machamer,C.E.;Doms,R.;Bole,D.G.;Helenius,A.;Rose,J.K. ] / J. Biol. Chem.
7	Catalysis of the oxidative folding of ribonuclease A by protein disulphide isomerase: Dependence of the rate on the composition of the redox buffer / [ Lyles,M.M.;Gilbert,H.F. ] / Biochemistry DOI ScienceOn
8	Monoclonal antibody productivity and the metabolic pattern of perfusion cultures under varying oxygen tensions / [ Shi,Y.;Ryu,D.D.Y.;Park,S.H. ] / Biotechnol. Bioeng. DOI ScienceOn
9	Protein disulphide-isomerase and the formation of native disulphide bonds / [ Freedman,R.B.;Brockway,B.E.;Lambert,N. ] / Biochem. Soc. Trans. DOI
10	Kinetics of recombinant immunoglobulin production by mammalian cells in continuous culture / [ Robinson,D.K.;Memmert,K.W. ] / Biotechnol. Bioeng. DOI
11	Redox properties and cross-linking of the dithiol/disulphide active sites of mammalian protein Disulphide Isomerase / [ Hawkins,H.C.;Nardi,M.;Freedman,R.B. ] / Biochem. J. DOI
12	Kinetics and specificity of homogeneous protein disulphideisomerase in protein disulphide isomerization and in thiol-protein-disulphide oxidoreduction / [ Lambert,N.;Freedman,R.B. ] / Biochem. J. DOI
13	Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the golgi / [ Lodish,H.F. ] / J. Biol. Chem.
14	Role of protein disulphide isomerase in the expression of native proteins / [ Freedman,R.B.;Bulleid,N.J.;Hawkins,H.C.;Paver,J.A. ] / Biochem. Soc. Symp.
15	An in vitro system for studying the kinetics of interchain disulphide bond formation in immunoglobulin G / [ Peterson,J.G.;Dorrington,K.J. ] / J. Biol. Chem.
16	Protein disulfide isomerase: A multifunctional protein resident in the lumen of the endoplasmic reticulum / [ Noiva,R.;Lennarz,W.J. ] / J. Biol. Chem.
17	Protein oligomerization in the ER / [ Hurtley,S.M.;Helenius,A. ] / Ann. Res. Cell Biol. DOI ScienceOn
18	Structural properties of homogeneous protein disulphide-isomerase from bovine liver purified by a rapid high-yielding procedure / [ Lambert,N.;Freedman,R.B. ] / Biochem. J. DOI
19	Preparation and characterization of monoclonal antibody to cephalexin-synthesizing enzyme from Acetobacter turbidans / [ Yang,C.Y.;Ryu,Y.W.;Ryu,D.D.Y. ] / Hybridoma DOI
20	Protein disulfide isomerase: Multiple roles in the modification of nascent secretory proteins / [ Freedman,R.B. ] / Cell DOI ScienceOn
21	Misfolding and aggregation of newly synthesized proteins in the endoplasmic reticulum / [ Marquardt,T.;Helenius,A. ] / J. Cell Biol. DOI ScienceOn
22	Performance of mammalian cell culture bioreactor with a new impeller design / [ Shi,Y.;Ryu,D.D.Y.;Park,S.H. ] / Biotechnol. Bioeng. DOI
23	Endoplasmic reticulum degradation of a subunit of the asialoglycoprotein receptor in vitro / [ Wikstrm,L.;Lodish,H.F. ] / J. Biol. Chem.
24	Catalysis of thiol/disulfide exchange: Single-turnover reduction of protein disulfide isomerase by glutathione and catalysis of peptide disulfide reduction / [ Gilbert,H.F. ] / Biochemistry DOI ScienceOn
25	The reactivities and ionization properties of the active-site dithiol groups of mammalian protein disulphide isomerase / [ Hawkins,H.C.;Freedman,R.B. ] / Biochem. J. DOI
26	Protein folding in the cell / [ Gething,M.;Sambrook,J. ] / Nature DOI ScienceOn
27	Catalysis of the oxidative folding of ribonuclease A by protein disulphide isomerase: Pre-steady-state kinetics and the utilization of the oxidizing equivalents of the isomerase / [ Lyles,M.M.;Gilbert,H.F. ] / Biochemistry DOI ScienceOn
28	Comparison of the activities of protein disulphide isomerase and thioredoxin in catalysing disulphide isomerization in a protein substrate / [ Hawkins,H.C.;Backburn,E.C.;Freedman,R.B. ] / Biochem. J. DOI
29	Kinetic study of hybridoma cell growth in continuous culture:Ⅱ.Behavior of producers and comparison to nonproducers / [ Frame,K.K.;Hu,W. ] / Biotechnol. Bioeng. DOI
30	Hepatoma secretory proteins migrate from rough endoplasmic reticulum to golgi at characteristic rates / [ Lodish,H.F.;Kong,N.;Snider,M.;Strous,G. ] / Nature DOI ScienceOn
31	/ [ Seinfeld,J.H.;Lapidus,L. ] / Mathematical methods in chemical engineering: Vol.3. Process modeling, estimation, and identification