• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.10
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• pp.831-835
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• 2008

We present a continuous electrical cell lysis chip, using a DC bias voltage to generate the focused high electric field for cell lysis as well as the electroosmotic flow for cell transport. The previous cell lysis chips apply an AC voltage between micro-gap electrodes for cell lysis and use pumps or valves for cell transport. The present DC chip generates high electrical field by reducing the width of the channel between a DC electrode pair, while the previous AC chips reducing the gap between an AC electrode pair. The present chip performs continuous cell pumping without using additional flow source, while the previous chips need additional pumps or valves for the discontinuous cell loading and unloading in the lysis chambers. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present chip generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100% lysis rate of Red Blood Cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of $30{\mu}m/s{\pm}9{\mu}m/s$. In conclusion, the present chip is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.04a
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• pp.993-997
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• 1997

The continuous chip depresses the accuracy of workpieces and promotes the wear of machine tools and hunts operators. So chip control os a major problem in turning process. In this paper, a method of chip identification is develope by pyrometer. The identifier is applied in real-time control of chip pattern with adjusting feedrate.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.16 no.3
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• pp.64-74
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• 2007

The continuous chip in turning operation deteriorates precision of workpiece and causes a hazardous condition to operator. Thus the chip form control becomes a very important task for reliable machining process. So, grooved chip breaker is widely used to obtain reliable discontinuous chip. However, developing new cutting insert having chip breaker takes long time and needs lots of research expense due to a couple of processes such as forming, sintering, grinding and coating of product and many different evaluation tests. In this paper, performance of commercial chip breaker is evaluated with neural network which is learned with a back propagation algorithm. For the evaluation, several important elements(depth of cut, land, breadth, radius) which directly influence the chip formation were chosen among commercial chip breakers and were used as input values of neural network. With the results of these input values, the performance evaluation method was developed and applied that method to the commercial tools.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.9
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• pp.191-199
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• 1999

In the continuous cutting process such as turning operation, chip control is thought very important to achieve the unmanned manufacturing system. The prediction of chip breakage under the given conditions is a substantial element for chip control. In this paper, a systematic approach to know the chip breaking region is represented under the concept of equivalent parameters. to Verify the suggested model, cutting experiments are executed with a commercial type and two other type chip breakers which have modified chip breaker parameters such as land width, groove width and nose radius. predicted chip breaking regions using the 3D cutting model agrees with those obtained from the experiments.

• Journal of the Korean Society for Precision Engineering
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• v.11 no.6
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• pp.42-49
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• 1994

One of the parameters that influence the productivity of every industry, involved in metal cutting, is the chip from ; continuous or broken chip. Chip form varies according to machining conditions, material used, tool geometry and chip breaker geometry. Therefore, in this study we carried out the experiment on the chip control in machining STS304 using an attached obstruction type chip breaker. Namely, with the change of a chip breaker distance, chip breaker angle, cutting characteristics in machining STS304 which is well-known as a machining difficult material and produces a saw-toothed chip. The results of the experiment are as follows : 1. The chip breaker distance and angle under which the preferred chip is produced, show 1.5mm and 60 .deg. , while chip breaker angle in machining an ordinary steel was well-known 45 .deg. . 2. During the cutting process, the change of feed than the change of velocity was applied as cutting conditions, effects more clearly on the chip breaking. 3. Considering a whole surface roughness, it is not advisable to apply chip breaker mentioned above for precision cutting.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2002.07a
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• pp.73-78
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• 2002

Microfluidic lab-on-a-chip (LOC) systems have enabled a new generation ofassay technologies in chemical and biomedical sciences. Caliper's microfluidic LOC systems contain a network of microscopic channels through which fluids and chemical are moved in order to perform experiments. The main advantages of these continuous-flow devices are integration and automation of multiple steps in complex analytical procedures to improve the reproducibility of the results, and eliminated the manual labor, time and pipetting errors involved in analyses. The present talk is devoted to give a brief introduction of microfluidic basics and to present in applying continuous-flow microchips to drug screening with model enzyme assays.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2692-2695
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• 2008

Polydiacetylene (PDA) is chemosensor materials that exhibit non-fluorescent-to-fluorescent transition as well as blue-to-red visible color change upon chemical or thermal stress. They have been studied in forms of film or microarray chip, so far. In this paper, we provide a novel technique to fabricate continuous micro-fiber PDA sensor using in-situ laser-polymerization technique and 3-D hydrodynamic focusing on a microfluidic chip. The flow of a monomer solution with diacetylene (DA) monomer is focused by a sheath flow on a 3-D microfluidic chip. The focused flow is exposed to 365 nm UV laser beam for in-situ polymerization which generates a continuous fiber containing DA monomers. Then, the fiber is exposed to 254 nm UV light to polymerize DA monomers to PDA. Preliminary results indicate that the fiber size can be controlled by the flow rates of the monomer solution and sheath flows and that a PDA sensor fiber successively responds to chemical and thermal stress.

• 연구논문집
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• s.33
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• pp.17-25
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• 2003

In this work, thermal design of a PCR chip for LOC is systematically conducted. From the numerical simulation of a PCR chip based on the finite volume method, how to control the average temperature of a PCR chip and the temperature difference between the denaturation zone and the annealing zone is presented. The average temperature is shown to be controlled by adjusting heat input and a cooler as well as a heater is shown to be necessary to obtain three individual temperature zones for polymerase chain reaction. To reduce the time required, a heat sink for the cooler is not included in the calculation domain for the PCR chip and heat sink design is conducted separately by using a compact modeling method, the porous medium approach.

• Proceedings of the KIEE Conference
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• 2006.07c
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• pp.1386-1387
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• 2006

PDMS와 ITO 유리를 이용하여 continuous-flow PCR chip을 제작하였다. PDMS를 이용하여 microchannel을 형성하여 주었고, ITO electrode를 heater와 sensor로 사용하기 위하여 반도체 공정을 통해 패턴을 형성하였다. microchannel내에 흐르는 시료의 온도를 제어하기 위하여 heater와 sensor를 calibration을 하였다. ITO heater는 인가된 전압에 대해 매우 선형적인 발열을 하였으며, ITO sensor는 온도에 대해 선형적인 저항 변화를 나타낸 바, 그 결과 continuous-flow PCR chip의 정확한 온도 제어가 가능하였다.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.8
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• pp.53-62
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• 1995

In recent years, the rapid development of the machine tool and tough insert has made metal removal rates increase, and automatic system without human supervision requires a higher degree reliability of machining process. Therefore the control of chips is one of the important topics which deserves much attention. The chip classification was made based upon standard deviation of the mean cutting force measured by a tool dynamometer. STS304was chosen as the workpiece which is known as the difficult-to-cut material and mainly saw-toothed chip produced, and the chip type according to the standard deviation of mean cutting force was classified into five categories in this experiment. Long continuous type chip which interrupts the normal cutting process, and damages the operator, tool and workpiece has low standard deviation value, while short broken type chip, which is favourable chip for disposal, has relatively large standard deviation value. In addition, we investigated the possibility that the chip type can be predicted analyzing the relationship between chip type and cutting condition by the trained neural network, and obtained favourable results by which the chip type can be predicted with cutting conditon before cutting process.