• 한국식품저장유통학회지
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• 제22권4호
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• pp.553-558
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• 2015

돌산갓에서의 glucosinolates인 sinigrin의 분석법을 확립하기 위해 다양한 용매를 이용하여 추출 후 정성 정량하였다. 돌산갓을 뿌리, 줄기, 잎으로 구분하여 50% $CH_3CN$, 10% $NH_4Cl$, 60% $CH_2OH$, 70% $CH_3OH$ 을 이용해 추출시 50% $CH_3CN$이 가장 sinigrin 함량이 높게 나타났으며 나머지 용매들은 큰 차이가 없었다. 또한 갓 부위별에서 sinigrin함량은 큰 차이가 없었으며 줄기 부위의 50% $CH_3CN$추출물의 sinigrin함량이 728 ppm으로 다소 높게 나타났다. Sinigrin은 농도별로 standard curve를 작성하여 HPLC에 의한 돌산갓 부위별 sinigrin 함량을 정량화 하였고 HPLC로 분석시 UV detector 감도는 242 nm보다 228 nm에서 높게 나타났으며 228 nm에서는 줄기, 뿌리, 잎 순서로 sinigrin함량이 나타났다. 50% $CH_3CN$로 추출한 돌산갓의 chromatogram은 표준물질인 sinigrin의 retention time과 일치하였으며, 잎에서 나타난 peak는 glucosinolates인 glucoraphanin으로 확인되었다. 본 연구의 결과로부터 돌산갓에 함유된 sinigrin 분석을 위한 추출용매는 50% $CH_3CN$, UV detector 파장은 228 nm가 가장 적합하다는 것을 알 수 있었다. 또한, sinigrin의 추출 및 분석이 체계화됨으로써 돌산갓을 이용한 다양한 식품의 저장기간에 따른 sinigrin 함량변화 및 생리기능에 관한 연구에 활용 될 수 있을 것으로 판단된다.

• 대한화장품학회지
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• 제48권3호
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• pp.255-264
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• 2022

자생 희귀콩의 한 종류인 납떼기콩(Glycine max landrace)의 활성 성분을 확인하고 이의 친환경적인 추출 방법으로 초음파 추출 방법을 도입하였다. 납떼기콩 추출물에 대한 성분 분석을 통해 기존 일반콩에서는 발견되지 않은 에피카테킨 성분을 확인하였다. 초음파를 활용한 효과적인 추출을 위해 통계학적 분석 방법인 반응 표면 분석법을 이용하여 주요 추출 조건을 최적화를 수행하였다. Box-Behnken design 프로세스를 통해 추출온도, 추출용매/용질의 비율, 추출 시간을 핵심 독립 변수로 하는 15가지 실험이 수행되었다. 두 가지 종속변수인 에피카테킨 함량과 총 이소플라본 함량에 대한 2차 회귀식과 이의 결정 계수는 각각 R² = 0.9939 와 R² = 0.9844로 높게 확인되어 상호 관계가 높은 유의성을 보이는 것으로 확인되었다. 이 두 가지 종속 변수의 최대 기대치를 만족하는 추출 조건은 추출온도 40.4 ℃, 추출용매/용질의 비율 19.3 times 및 추출 시간 91 sec 로 예측되었다. 이에 대한 에피카테킨 함량과 총 이소플라본 함량의 기댓값과 실제 실험값이 유사한 결과로 확인되어 높은 신뢰성이 있는 최적화 모델임을 확인하였다.

• KSBB Journal
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• 제18권3호
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• pp.202-206
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• 2003

역미셀과 가압이산화탄소를 이용한 난백 lysozyme의 추출실험에서 일정한 압력의 이산화탄소를 유기용액에 가하였을 때 lysozyme의 추출율과 그 때 형성되는 역미셀의 크기, 즉 역미 셀 내부의 수분함량 ($W_{0}$)를 알아보았다. 이산화탄소로 가압된 상태에서도 수용액에서 유기용액으로의 lysozyme의 추출 경향은 실험조건인 수용액의 이온강도, pH, 유기용액의 계면활성제의 농도를 달리 하였을 때 기존의 다른 연구와 유사한 경향을 나타내었으며, 이산화탄소의 압력을 102 bar까지 증가시켰을 때 가장 높은 추출율을 나타내었다. 또한 이때 역미셀 내부의 수분함량 ($W_{0}$)을 측정한 결과, lysozyme의 추출율과 역미셀 내부의 수분함량은 비례함을 확인할 수 있었다.

• 한국약용작물학회지
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• 제21권4호
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• pp.276-281
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• 2013

This study was conducted to compare the contents of ginsenoside according the water extract conditions of red ginseng. In method A, red ginseng extract was prepared at $75^{\circ}C$ for 18 hours by 1 time extraction, and method B, the preparation was done at $85^{\circ}C$ for 18 hours by 1 time extraction. In method C, the primary extract prepared at $75^{\circ}C$ for 9 hours was blended with the secondary extract prepared by re-extracting the red ginseng residue obtained after the primary extraction, at $85^{\circ}C$ for 9 hours. Method D was the same procedure as method C but the extraction temperature for the primary extraction was $85^{\circ}C$ and that for the secondary extraction was $95^{\circ}C$. The contents of total and $Rb_1$, $Rg_1$ and $Rg_3$ ginsenoside were highest in Method C. The content of prosapogenin (ginsenoside $Rg_2$, $Rg_3$, $Rb_1$ and $Rb_2$) was highest in Method B. There was no consistent tendency in Brix, pH, Hue value and absorbance among extraction methods.

• 한국식품조리과학회지
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• 제7권1호
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• pp.27-34
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• 1991

This study attempted to set up reasonable extraction time of Omija that was put in water for the various components to soak out. Changes of free sugars, organic acids, reducing sugar, total acid and tannin in Omija with various extraction times were investigated (together with the analysis of each components in Omija fruit). 1. High Performance Liquid Chromatography showed fructose, glucose, and sucrose to be the major free sugars of the Omija fruit. Free sugars and reducing sugar value in Omija beverage increased gradually in according with the extraction time, and marked 75.6% per total free sugars and 82.1% per total reducing sugar at 12 hours. 2. Gas Chromatography showed lactic acid, oxalic acid, fumaric acid, levulinic acid, succinic acid, malic acid, citric acid and pyroglutamic acid to be the major organic acids of the Omija fruit. Organic acids and total acids value in Omija beverage increased gradually on proportion to extraction time, and marked 97.0% per total organic acids at 9 hours and 79.0% per total acids at 12 hours. 3. Tannin content in Omija beverage was increased when extraction time was longer but it showed a low percentage as compared with the reducing sugar and total acid. Tannin content marked 48.8% per total tannin at 12 hours. 4. Sensory evaluation revealed that !1 hours of extraction produced the best quality products based in taste, flavor, color and over-all acceptability, considering the data, it seems possible to conclude that the optimum of time for extraction of Omija to water is 9 hours.

• 정보관리학회지
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• 제26권1호
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• pp.305-320
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• 2009

웹을 통해 제공되는 뉴스 페이지의 경우 필요한 정보 뿐 아니라 많은 불필요한 정보를 담고 있다. 이러한 불필요한 정보는 뉴스를 처리하는 시스템의 성능 저하와 비효율성을 가져온다. 이 연구에서는 웹 페이지로부터 뉴스 콘텐츠를 추출하기 위해 문장과 블록에 기반한 뉴스 기사 추출 방법을 제시하였다. 또한 이들을 결합하여 최적의 성능을 가져올 수 있는 방안을 모색하였다. 실험 결과, 웹 페이지에 대해 하이퍼링크 텍스트를 제거한 후 문장을 이용한 추출 방법을 적용하였을 때 효과적이었으며, 여기에 블록을 이용한 추출 방법과 결합하였을 때 더 좋은 결과를 가져왔다. 문장을 이용한 추출 방법은 추출 재현율을 높여주는 효과가 있는 것으로 나타났다.

• Journal of the Korean Wood Science and Technology
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• 제46권4호
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• pp.380-392
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• 2018

The importance of polysaccharides is increasing globally due to their role as a significant source of dietary prebiotics in the human diet. In the present study, in order to maximize the yield of crude polysaccharides from Pinus densiflora, response surface methodology (RSM) was used to optimize a two-stage extraction process consisting of steam explosion and water extraction. Three independent main variables, namely, the severity factor (Ro) for the steam explosion process, the water extraction temperature ($^{\circ}C$), and the ratio of water to raw material (v/w), were studied with respect to prebiotic sugar content. A Box-Behnken design was created on the basis of the results of these single-factor tests. The experimental data were fitted to a second-order polynomial equation for multiple regression analysis and examined using the appropriate statistical methods. The data showed that both the severity factor (Ro) and the ratio of water to material (v/w) had significant effects on the prebiotic sugar content. The optimal conditions for the two-stage process were as follows: a severity factor (Ro) of 3.86, a water extraction temperature of $89.66^{\circ}C$, and a ratio of water to material (v/w) of 39.20. Under these conditions, the prebiotic sugar content in the extract was 332.45 mg/g.

• 한국수산과학회지
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• 제54권1호
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• pp.9-15
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• 2021

Sargassum serratifolium is a rich source of antioxidant meroterpenoids. The optimal extraction temperature and time for obtaining maximal antioxidant yield, antioxidant activity, and phenolic content from Sargassum serratifolium were determined using response surface methodology (RSM). The ranges of the independent variables for extraction temperature and time were 30-70℃ and 12-36 h, respectively. With increasing temperature and time, the yield increased significantly, while DPPH (2,2-dipheny-1-picrylhydrazyl) radical-scavenging activity and total phenolic content decreased significantly. The optimal extraction temperature and time obtained by RSM were 54℃ and 7 h, respectively, providing a yield of 8.2%, DPPH radical-scavenging activity of 60%, and total phenolic content of 163 mg GAE/g. The findings of this study provide useful information for the development of Sargassum serratifolium extraction processes for the food and cosmetic industries.

• 동의생리병리학회지
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• 제36권2호
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• pp.54-58
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• 2022

This study aimed to investigate the anti-stress function of Aucklandiae Radix (AR) and Cyperi Rhizoma (CR). The essential oils used in the experiment were extracted from AR and CR using Steam Distillation Extraction and Super critical CO₂ extraction. To observe the effects of sample administration, we measured feed intake, leukocytes, red blood cells, hemoglobin, platelets, serum serotonin content, immobility time, climbing time, and swimming time in mice subjected to chronic restraint stress as behavioral changes. The average body weight of all experimental groups increased than the average body weight of the control group. The immobility and climbing times of experimental groups A and B administered with supercritical extraction samples were shorter than those of the other experimental groups and the control group, and the swimming time was longer. The serotonin content in the blood of all experimental groups decreased compared to the normal group, and the serotonin content of the control group was increased. The authors suggest that Korean herbal medicines AR and CR may be utilized as anti-stress flavoring agents based on the above results.

• 한국멀티미디어학회논문지
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• 제15권6호
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• pp.693-700
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• 2012

The effective extraction of key frames from a video stream is an essential task for summarizing and representing the content of a video. Accordingly, this paper proposes a new and fast method for extracting key frames from a compressed video. In the proposed approach, after the entire video sequence has been segmented into elementary content units, called shots, key frame extraction is performed by first assigning the number of key frames to each shot, and then distributing the key frames over the shot using a probabilistic approach to locate the optimal position of the key frames. The main advantage of the proposed method is that no time-consuming computations are needed for distributing the key frames within the shots and the procedure for key frame extraction is completely automatic. Furthermore, the set of key frames is independent of any subjective thresholds or manually set parameters.