Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Optimization of Polyphenol Extraction Process from Native Soybean using Ultrasound

자생 희귀콩인 납떼기콩으로부터 초음파를 이용한 폴리페놀 성분의 추출 공정 최적화

  • Kang, Hye Jung (Department of Engineering Chemistry, Chungbuk National University, College of Engineering) ;
  • Park, Junseong (Department of Engineering Chemistry, Chungbuk National University, College of Engineering)
  • 강혜정 (충북대학교 공업화학과) ;
  • 박준성 (충북대학교 공업화학과)
  • Received : 2022.08.23
  • Accepted : 2022.09.26
  • Published : 2022.09.30
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Abstract

The active ingredients of Napttegi Kong(GML, Glycine max landrace), a type of native rare soybeans, were identified, and an ultrasonic extraction method was introduced as an eco-friendly extraction method. Through the component analysis of the Napttegi Kong extract, the epicatechin, which was not found in conventional soybeans, was identified. For effective extraction using ultrasonic, the main extraction conditions were optimized using the response surface analysis method. Through the Box-Behnken design process, 15 experiments were conducted with the extraction temperature, the ratio of extraction solvent/solution, and extraction time as key independent variables. A quadratic regression equation for the two dependent variables, epicatechin content and total isoflavone content, was derived, and the coefficients of determination were found to be high as R2 = 0.9939 and R2 = 0.9844, respectively, confirming that the correlation showed high significance. The extraction conditions satisfying the maximum expectations of these two dependent variables were predicted. to be 40.4℃ of extraction temperature, 19.3 times of extraction solvent/solution, and 91 sec of extraction time. The expected value and the actual experimental value of the epikatechin content and the total isoflavone content were similar, so it was confirmed that this experimental method is a highly reliable optimization model.

자생 희귀콩의 한 종류인 납떼기콩(Glycine max landrace)의 활성 성분을 확인하고 이의 친환경적인 추출 방법으로 초음파 추출 방법을 도입하였다. 납떼기콩 추출물에 대한 성분 분석을 통해 기존 일반콩에서는 발견되지 않은 에피카테킨 성분을 확인하였다. 초음파를 활용한 효과적인 추출을 위해 통계학적 분석 방법인 반응 표면 분석법을 이용하여 주요 추출 조건을 최적화를 수행하였다. Box-Behnken design 프로세스를 통해 추출온도, 추출용매/용질의 비율, 추출 시간을 핵심 독립 변수로 하는 15가지 실험이 수행되었다. 두 가지 종속변수인 에피카테킨 함량과 총 이소플라본 함량에 대한 2차 회귀식과 이의 결정 계수는 각각 R2 = 0.9939 와 R2 = 0.9844로 높게 확인되어 상호 관계가 높은 유의성을 보이는 것으로 확인되었다. 이 두 가지 종속 변수의 최대 기대치를 만족하는 추출 조건은 추출온도 40.4 ℃, 추출용매/용질의 비율 19.3 times 및 추출 시간 91 sec 로 예측되었다. 이에 대한 에피카테킨 함량과 총 이소플라본 함량의 기댓값과 실제 실험값이 유사한 결과로 확인되어 높은 신뢰성이 있는 최적화 모델임을 확인하였다.

Keywords

Acknowledgement

이 논문은 환경부의 재원으로 한국환경산업기술원의 야생생물 유래 친환경 신소재 및 공정 기술개발사업사업의 지원을 받아 연구되었습니다.(2021003280001)

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