• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.297-305
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• 2008

A series of experiments were conducted to suggest efficient in vitro germination conditions to improve germination rate and aseptic seedling production in chicory and garland chrysanthemum. For this purpose, various levels of NaOCl concentration and soaking treatment time combinations were tasted. Fifteen minutes of soaking treatment with 5% NaOCl solution significantly improved germination and seedling formation percentages and prevented contamination in both chicory 'Precole' and 'Chiavari' and garland chrysanthemum 'Okiku 3'. There was no significant difference in germination response between MS and 1/2 MS medium compositions, but germination and seedling formation were delayed as sucrose concentration increased. Although petri-dish among various culture containers gave rise to improved germination responses in chicory 'Precole', there was no significant difference in germination responses among culture container types in chicory 'Chiavari' and garland chrysanthemum 'Okiku 3'. The ultrasonic treatment stimulated germination and seedling formation in both chicory cultivars but there was no effect in garland chrysanthemum 'Okiku 3'. The results suggested 15 minutes with 5% NaOCl for seed sterilization, 1/2 MS medium with no sucrose and petri-dish as proper medium composition and culture container, and 120 minutes with 5% NaOCl solution of ultrasonic treatment as promoting method for in vitro germination in chicory and garland chrysanthemum.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.243-252
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• 2015

This study was performed to establish a production system for in situ and ex situ conservation of Elsholtzia minima Nakai, an endemic plant grown in Jeju Island. Moreover, this study aimed to identify root-growth characteristics according to the use of pre-treatment agents and seedling growth effect according to fertilizer application. The mean temperature was similar in greenhouse and vinyl-moist chamber, but air humidity was higher in vinyl-moist chamber than in greenhouse. After stem planting of Elsholtzia minima Nakai, initial root growth was observed after 10 days in greenhouse and after 7 days in vinyl-moist chamber. Root growth rate was more rapid in vinyl-moist chamber. Moreover, survival rate, root growth rate and root number was slightly higher in vinyl-moist chamber than in greenhouse, indicating that vinyl-moist chamber is more effective in plant growth. When pre-treatment agents were used to remove root growth-inhibiting substances, a higher root growth rate of more than 95% was found in pre-treatment groups, excluding the group treated with AgNO₃ at 77.5%. Thus, Elsholtzia minima Nakai is thought to have less root growth inhibitors. In the analysis of nitrogen application rate and Osmocote application by seedling container, a difference was found in survival rate and growth according to application rate and container conditions. When Osmocote, a slow release fertilizer, was applied to the soil surface around each culture container, survival rate and the growth of aerial and root parts were most favorable. Thus, Osmocote fertilizer is thought to be desirable for seedling propagation of Elsholtzia minima Nakai.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.394-404
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• 2022

This study was conducted to optimize the amount of drip irrigation for Prunus×yedoensis Matsum., one of the major medium-sized landscaping trees used mainly for streetscapes and as ornamentals. The experiment was conducted in a container nursery, where we assessed the growth and physiological characteristics of 4- and 8-year-old seedlings watered at different rates (x) by a drip irrigation system. The relative growth rates (based on height and root collar diameter (RCD) measurements) were highest at 288 and 416 L/year/tree for the 4- and 8-year-old containerized seedlings, respectively. These age and treatment combinations also produced significantly different dry weight and seedling quality index values, indicating good growth. The two age groups had significantly different total root lengths, root diameters, and root volumes under these respective irrigation treatments. In addition, the 4-year-old containerized seedlings irrigated at 288 L/year/tree and the 8-year-old containerized seedlings irrigated at 416 L/year/tree had the highest activations in their chlorophyll contents. Overall, the results (differences in irrigation amounts affect the seedlings morphological relative growth, biomass growth, seedling quality, and physiological reaction) indicate that the optimal irrigation amounts for container-grown Prunus×yedoensis are 288 L/year/tree for 4-year-old (RCD class, 3cm) and 416 L/year/tree for 8-year-old (RCD class, 7 cm) containerized seedlings.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.93-102
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• 2019

The materials of artificial soils in the production process of container seedlings have a great influence on plant growth. Peat moss, vermiculite, and perlite have been used as major components of artificial soils for many years; however, they could decrease carbon fixation carried out by the soil and cause environmental problems such as a change in the water quality. Thus, environmental friendly materials to replace them must be developed. The purpose of this study was to verify the optimum additive materials of artificial soils and their mixed ratios for the growth and seedling quality index (SQI) of Quercus serrata. Rice husk, mushroom sawdust, and pine bark were each used as an additive material and mixed into the growth medium at 10% and 20% of the total volume. There was no significant difference in the height growth of Q. serrata. The 20% mushroom sawdust decreased the root collar diameter by 23.4% compared to the control. The total dry weight was highest with the 10% rice husk and was significantly lower by 10.3% for the 20% mushroom sawdust compared to the control. Additionally, the SQI for all the treatments showed no tendency to increase compared with the control. Thus, this study showed the possibility of recycling biomaterials from agriculture and forest for seedling production. This method could reduce environmental problems and help eco-friendly nurseries to achieve a carbon negative impact by the recycling of by-products.

• Korean Journal of Agricultural Science
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• v.45 no.2
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• pp.143-153
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• 2018

Soil characteristics along with various container lengths have an important role in the early survival rate and growth of seedlings by influencing the seedling quality. This experiment was conducted to investigate the effect of container length and different soil mixtures on the growth of poplar in a greenhouse. Two types of soil, homogeneous vs. heterogeneous, were used along with two container lengths (30 vs. 60 cm). The heterogeneous soil was made by dividing 50% vermiculite from a mixture of 25% vermicompost and 25% nursery soil in volume. For the homogeneous soil, the above three soil types were mixed together. Populus euramericana clone cuttings were planted in late April, and then, the growth height, root collar diameter (RCD) and biomass were measured in August. The height of the poplar was not significantly affected by container length and soil type, but the RCD was significantly affected by soil type. Leaf and root biomass was higher at the long container than at the short container for both soil treatments, but stem biomass was lower at the heterogeneous soil than at the homogeneous soil treatment. Root to shoot biomass ratio was higher at the heterogeneous soil treatment than at the homogeneous soil treatment by 12%. In conclusion, heterogeneous soil along with a long container is suitable to increase the carbon allocation into the root.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.26 no.1
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• pp.61-68
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• 2023

In North Korea, the production of courage tombs continues, and it is known that the activity rate is higher than that of old tombs. However, pot seedling are planted using hoes and shovels used for planting old tombs with exposed roots and low activity rates. This is believed to result in excessive force when stepping on the container grave with a hoe in the planting process, resulting in the collapse of the container grave or the waste of labor due to the creation of unnecessary planting holes. Therefore, when planting courage graves at North Korean afforestation sites, it is necessary to improve the work of making planting holes using general hoes in a way that improves labor productivity in a more efficient manner. As part of inter-Korean technical cooperation to improve the North Korean afforestation method, this study was conducted with the aim of developing efficient container seedlings and using them for North Korean forest restoration projects. It is believed that developing planting equipment exclusively for container graves for forest restoration in North Korea in South Korea and providing equipment and production technology to North Korea can contribute to the development of forest restoration technology in North Korea. If the Yonggeomyo Development Planting Equipment is provided to North Korea, it will be a realistic inter-Korean forest cooperation project to avoid international sanctions by recognizing the excellence of the development products by directly using its own materials through technical cooperation.

• Plant Resources
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• v.8 no.3
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• pp.286-292
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• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.390-396
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• 2015

This study was carried out in order to closely examine quality index by shading level of 2-year-old (1-1 seedling) container seedling of Daphniphyllum macropodum which is known as the species of having shade tolerance that is evergreen broad leaved tree in the warm temperate region. The shading treatment was regulated with the shading level of full sunlight, and 35%, 55%, 75%, 95% of full sunlight. As a result of surveying growth according to the shading level, both height and root collar diameter were surveyed to be the highest with 45.1 cm and 8.22 mm, respectively, under 75% of shading. The next was surveyed to be 43.2 cm & 8.05 mm and 42.5 cm & 7.98 mm, respectively, in order of 35% and 55% in shading. Leaf, shoot, root, and whole dry mass production were the highest under 75% of shading. The next was higher in leaf, stem, and whole dry mass production under 55% of shading. A root was higher under 35% of shading in the next. H/D ratio was the range of 5.29~5.35 under the 35~75% shading that showed the relatively high height and root collar diameter. T/R ratio was the lowest with 1.17 under 35% of shading. It was 0.41 under 95% of shading as for LWR, 0.24 under 75%-95% of shading as for SWR, and 0.46 under full sun and 35% shading as for RWR. QI was the highest with 3.74 under 75% of shading. As a result of surveying the whole experiment, it is concluded that the production of D. macropodum seedling is more effective under 75% shading

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.470-477
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• 2021

Amsonia ellipotica (Thumb.) Roem. & Schult. has less than 10 native places nationwide and is evaluated as an endangered species (EN) on the Korean Red List, so systematic breeding and cultivation methods are required. This study was carried out to establish an effective method for seedling production in A. elliopotica that can be developed as a genetic resource conservation and new ornamental material. This experiment was performed by varying the seedling production container (72, 105 and 128 cell trays), number of seeds sown in a cell (1, 2, and 4 per cell), soil type [horticultural soil and neutralized peat-moss: perlite mixed in 3:1, 4:1 (v:v)], additional fertilizer concentrations (0, 250, 500 and 1000 mg/L) and shading degrees (0, 55 and 75%). As a result of the study, growth increased with the increased in measuring capacity per cell of sowing container. According to number of seeding growth, sowing one seed per cell was the best. Seedling growth increased as the treatment concentrations increased, higher growth was in 1000 mg/L. On the other hand, during the shading treatment, growth significantly decreased regardless of shading degrees.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.27 no.3
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• pp.181-186
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• 2021

The research was aimed to improve pre-harvest methods to maintain marketability in postharvest coniferous lettuce. Non-woven pots or plug plates were used to generate seedlings. No difference was found in growth characteristics of lettuce grown in non-woven pots vs plug plates. Since, seedling method with non-woven pots was convenient, lettuce harvested from non-woven pots used for water dipping treatment. The quality parameters, such as fresh weight loss, SPAD value, and appearance of lettuce were investigated after harvest. The lettuce with dipping treatment inside the plastic box container showed lower weight loss, higher SPAD value and better appearance compared to those exposed to the control (non-packaging) during the storage at 2℃. The treated plant showed higher SPAD and hue angle values 21.9 and 113.8°, respectively, compared to that of 18.8 and 108.3°, in non-treated plants. Therefore, it seems that the water dipping treatment is effective for storage method to maintain freshness of the lettuce. We showed the non-woven pot growing as a convenient seedling method for packaging treatment. Further studies will be continued to improve freshness postharvest of other horticultural crops.