• 해부∙생물인류학
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• 제31권4호
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• pp.143-149
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• 2018

접촉피부염은 피부가 이물질과 접촉함으로써 일어나는 염증반응으로 백혈구 혈관외유출이 매우 중요한 역할을 한다는 것은 잘 알려진 사실이다. 선행연구 결과 CD99가 ${\beta}1$ 인테그린 의존적 메커니즘을 통하여 단핵구의 혈관외유출을 조절하며, 따라서 염증 질환에 대한 치료제 개발의 신규 표적 분자일 가능성이 제시되었다. 본 연구에서는 CD99 유래 펩타이드인 CD99CRIII3가 단핵구의 혈관외유출과 접촉피부염 생쥐 모델에서 염증 반응을 억제하는지를 조사하였다. 인간 단핵구 세포주인 U937를 CD99CRIII로 처리할 경우 농도의존적으로 ${\beta}1$ integrin의 활성도가 감소되었으며, 이 세포주의 사람제대정맥내피세포에의 부착과 혈관외유출도 억제되었다. 나아가 CD99CRIII3는 포르볼 미리스테이트 아세테이트 처리에 의해 유발된 생쥐 접촉피부염 모델에서 Evans Blue의 혈관투과와 귀조직 무게를 농도 의존적으로 억제하였다. 이러한 결과들은 CD99CRIII3가 단핵구의 혈관외유출과 접촉피부염 동물 모델에서 일어나는 염증반응을 억제함을 보여준다. 이와 같이, 본 연구는 CD99 유래 펩타이드가 피부 염증질환에 대한 치료제로 개발될 가능성이 있음을 제시한다.

• 혜화의학회지
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• 제16권2호
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• pp.131-145
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• 2007

To evaluate the effects of GP on contact dermatitis, we examined the composition of immune cells from drain lymph node in DNCB-induced contact dermatitis murine model NC/Nga mice. And the amount of pathologic cytokines of spleen and antioxidant activity were investigated. The results were summarized as followers; 1. GP did not show cytotoxic effect on mLFC in vitro. 2. GP did not have hepatotoxicity in vivo in the level of ALT, AST. 3. GP decreased the production of DPPH and in a dose-dependent. 4. GP significantly decreased total cell number of DLN in DNCB-induced NC/Nga mice compared to the untreated control group. 5. GP significantly decreased the number of CD3+, CD19+, CD4+, CD8+, CD3+/CD69+ and CD4+/CD45+ in DLN of DNCB-induced NC/Nga mice compared to the untreated control group. 6. GP significantly reduced the level of IL-4 and IFN-$\gamma$ in splenocytes of DNCB-induced NC/Nga mice compared to the untreated control group. Taken together above results, GP have therapeutic effects on contact dermatitis by regulating T cell activation. This study warranted further investigations of molecular mechanisms of GP on contact dermatitis.

• 대한한방내과학회지
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• 제19권2호
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• pp.333-346
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• 1998

In order In study the effects of an immunosuppression and a mitigation of inflammation of Gamiseungmagalgeuntang(GMSG) on the allergic contact dermatitis, contact hypersensitivity assay, mast cell, VCAM-1, and T cell of the lymph node in mice induced allergic contact dermatitis by the contacts-sensitizing DNCB were obsereved after oral administration of GMSG extract. The results of this study were as follows ; 1. Ear swelling in contact hypersensitivity assay was decreased in the GMSG group as compared with DNCB group. 2. It was investigated that mast cells were appeared degranulated type in DNCB group and a number of granulated type was more GMSG group than DNCB group. 3. VCAM-1 expression in epidermis and CD11b positive cell in dermis were decreased In the GMSG group as compared with DNCB group. 4. In the lymph node ,the distribution of CD4, CD8, IL-2R positive cell were decreased at the cortex and medula in the GMSG group as compared with DNCB group.

• 동의생리병리학회지
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• 제31권1호
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• pp.59-64
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• 2017

Scutellaria baicalensis Georgi is one of major herbs used to treat patients with skin diseases diagnosed by wind-heat syndrome. This study was designed to investigate the effects of Scutellaria baicalensis Extract (SBE) on skin lesion of Contact dermatitis (CD) in mice. We investigated the effects of SBE on skin thickness, skin lesions, erythema index and melanin index in vivo. The effects on body weights and spleen/body weight ratio were also investigated in mice with CD induced by topical application of 1-Fluoro-2,4-dinitrofluorobenzene (DNFB). In our results, topical application of SBE lowered skin thickness significantly. In addition, SBE ameliorated skin lesions such as erythema, exudate and petechia in mice with CD. SBE also inhibited erythema index elevated by DNFB significantly. finally, SBE did not affect body weight and spleen/body weight ratio. In conclusion, these data imply that SBE can ameliorate skin lesion of CD such as erythema, exudate and petechia, and be used to treat CD patients with relative safety.

• 한방안이비인후피부과학회지
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• 제30권4호
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• pp.25-36
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• 2017

Objectives : The aim of this study is to report the effect of Capsosiphon fulvescens(Maesangi) extract(MSE) on contact dermatitis(CD). Methods : CD induced by Dermatophagoides farinae body(Dfb) in NC/Nga mice. 4% sodium dodecyl sulfate(SDS) $150{\mu}l$ and Dfb(10mg/ml) ointment 100mg were topically treated twice a week for four weeks. MSE 200 mg/kg was topically treated every 4weeks. Every week, we examined the skin lesions, weight of spleen and lymph node, epidermal thickness, production of Tumor necrosis factor-alpha($TNF-{\alpha}$) and interleukin-6(IL-6). Results : MSE reduced features of Dfb-induced skin lesions, weight of spleen and production of $TNF-{\alpha}$. MSE significantly reduced weight of lymph node, epidermal thickness, number of mast cells and production of IL-6. Conclusions : MSE may have a potential therapeutic effects for CD by suppressing allergic inflammation.

• 한방안이비인후피부과학회지
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• 제29권2호
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• pp.65-81
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• 2016

Objectives : Haedoksamul-tang (HSTE), a water extract from a mixture of Phellodendri Cortex, Coptidis, Scutellariae Radix, Gardeniae Fructus, Angelica acutiloba Radix, Cnidii Rhizoma, Paeoniae Radix, Rehmanniae Radix, has been traditionally used for allergic skin diseases such as atopic dermatitis and contact dermatitis in oriental countries. However, little is known about the effects of aqueous extract of HSTE on trimellitic anhydride (TMA)-induced contact hypersensitivity (CHS) in a mouse model. Methods : In this study, we investigate the pharmacological effects of HSTE on TMA-induced CHS in Balb/c mice. Contact hypersensitivity was induced in mice by topically sensitizing and challenging with TMA in flank skin and ears during oral administration (for 17 days) and topical treatment (30 min before challenge) with HSTE. We examined the effects of HSTE on IgE and IgG1 levels, inflammatory parameters in ear tissues, CD4⁺/CD8⁺ ratio, cytokine and chemokine production in sera, tissues, and immune cells from TMA-sensitized mice.Results : Oral and topical administration with HSTE reduced, in a dose dependent manner, thickness and leukocyte infiltration of ear tissues and IgE levels in serum from mice sensitized with TMA. In addition, auricula lymph node cells isolated from TMA-sensitized mice significantly elevated the expression ratio of CD4⁺/CD8⁺ as well as increased the production of IL-4, IL-5, IL-13 and IFN-γ by ex vivo stimulation with antibodies against CD3 and CD28, and these inflammatory indexes, except for IFN-γ, were significantly suppressed by orally and topically administration of HSTE. Furthermore, stimulation of auricula lymph node cells from TMA-sensitized mice with antibodies against CD3 and CD28 increased the production of MCP-1/CCL2 and MIP-1α/CCL3, and these effects were inhibited in a dose-dependent manner in cells from mice treated with HSTE. Conclusions : These results suggest that HSTE can be used for treating contact hypersensitivity by inhibiting leukocyte infiltration as well as production of serum IgE and chemokine/Th2 cytokine in an animal model.

• 대한상한금궤의학회지
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• 제8권1호
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• pp.53-65
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• 2016

Objective : The purpose of this paper is to report the improved contact dermatitis(CD) treated with the herbal medication based on Shanghanlun disease pattern identification diagnostic system. The level V steroid ointments were no use to the patient 3weeks before the treatment. Methods : In this case, the patient was diagnosed according to 'Disease Pattern Identification Diagnostic System based on Shanghanlun Provisions' and took Mahwang-tang for 36days since the final diagnosis was Taeyang-byung, number 46 provision. The photographs of patient's hands were taken to observe the process of the skin status and the discomfort from CD was measured with DLQI(Dermatologic Life Quality Index) and NRS(Numeric Rating Scale). Results : The DLQI score changed from 24 to 7 and NRS of itching changed form 8 to under 4. Conclusions : This case report shows that the herbal medication through the diagnostic system could be a remedy on CD, dealing with the skin status with no use of the level V steroid ointments. It is needed proving an alternative possibility of Korean Medicine, on other skin diseases, with later studies since there are many reports on the side-effects after some long-term steroid treatments.

• 동의생리병리학회지
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• 제26권5호
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• pp.693-698
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• 2012

This study was designed to investigate the effects of 1-fluoro-2,4 dinitrobenzene (DNFB) on Induction of dermatitis in mice. We investigated the effects of DNFB on induction of dermatitis in terms of changes in body weights, ear thickness, ear weight, spleen/body ratio, histopathological observation and cytokine productions in inflammed tissue of contact dermatitis (CD) mice. In our experiments, we induced CD by using two different methods. First, mice were sensitized and challenged on the back of each ear (topical induction). Second, mice were sensitized on shaved back and challenged back of each ear (systemic induction). In our results, average weights were lowered in both topical and systemic group. But, there was no statistical significance between topical and systemic group. Treatment with DNFB enlarged ear weights and thicknesses in both topical and systemic groups. In addition, both groups were showed almost same features such as immune cell infiltration, spongiosis and hyperplasia in histopathological observations. Finally production levels of TNF-a, IFN-g and IL-6 were markedly elevated in both topical and systemic groups. On the other hand, systemic induction was more effective in spleen/body ratio and IL-6 production compared to topical induction respectively. These results suggest that dermatitis model using DNFB was used as model of CD, not for atopic dermatitis model.

• 대한약침학회지
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• 제16권2호
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• pp.41-45
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• 2013

Objective: The root bark of Ulmus davidiana var. Japonica (Ulmi Radicis cortex, URC) is a medicinal herb used for promoting diuresis and treating dampness. In Korea, URC has long been used as an efficacious therapy for inflammation, burns, frostbite and skin diseases such as eczema and psoriasis. Methods: In the present study, we used 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) mouse model to investigate the anti-allergic and the anti-inflammatory effects of URC on skin lesion, histopathological changes and specific antibody production. Results: URC treatment, 10 mg/mL, effectively inhibited skin lesions induced by repeated paintings with DNFB. In the histopathological observation, topical application of URC inhibited spongiosis. In addition, URC lowered the production levels of total immunoglobulin and IgG2a in serum. Conclusion: These data indicate that URC has an anti-inflammatory effect that produces an improvement of skin lesions in CD mice.

• 동국한의학연구소논문집
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• 제7권1호
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• pp.119-133
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• 1998

본 실험은 황금이 Allergy성(性) 접촉피부염의 면역과민반응 및 염증반응에 미치는 영향을 조사하기 위해서 시행되었다. 실험을 위해 BALB/C계 생쥐를 DNCB 처리군과 DNCB 처리 후 황금추출액을 경구 투여한 황금추출물투여군(HGET군)으로 나누어 2,4-dinitrochlorobenzene(DNCB)를 도포하여 Allergy성(性) 접촉피부염을 유발한 뒤, 시간의 경과에 따라 contact hypersensitivity assay, 피부의 일반적인 구조, 혈관생성, 림프구를 비롯한 염증세포, sulfated acid mucosubstance, 비만세포, IL-2 R, ICAM-1 그리고 CD11b의 변화를 관찰하였다. Contact hypersensitivity assay에서 HEGT군은 DNCB처리군에 비해 유의성(有意性)있는 ear swellig의 감소를 보였다. 피부표피의 변화에서 HEGT군에서 DNCB처리군에 비해 감소된 것으로 나타났다. 또한 혈관생성, 림프구의 침투 및 표피 기저층과 가시층 세포의 손상은 HEGT군에서 DNCB처리군에 비해 감소된 것으로 나타났다. 또한 혈관생성, 림프구를 비롯한 염증세포, sulfated acid mucosubstance와 비만세포, IL-2 R 양성반응세포, ICAM-1 양성반응세포 그리고 CD11b 양성반응은 Allergy성(性) 접촉피부염의 면역과민반응억제와 항염증작용에 효과가 있는 것으로 사료된다.