Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of 1-fluoro-2,4 dinitrobenzene on Induction of Dermatitis in Mice

1-fluoro-2,4 dinitrobenzene처리 방법이 피부염 유발에 미치는 영향

  • Kim, Mi Young (School of Korean Medicine, Pusan National University) ;
  • Kim, Han Na (School of Korean Medicine, Pusan National University) ;
  • Lee, Mi Ran (School of Korean Medicine, Pusan National University) ;
  • Kim, Byung Joo (School of Korean Medicine, Pusan National University) ;
  • Choi, Chan Hun (College of Korean Medicine, Dongshin University) ;
  • Jeoung, Hyun Woo (College of Korean Medicine, Dongshin University) ;
  • Kim, Hyung Woo (School of Korean Medicine, Pusan National University)
  • 김미영 (부산대학교 한의학전문대학원) ;
  • 김한나 (부산대학교 한의학전문대학원) ;
  • 이미란 (부산대학교 한의학전문대학원) ;
  • 김병주 (부산대학교 한의학전문대학원) ;
  • 최찬헌 (동신대학교 한의과대학) ;
  • 정현우 (동신대학교 한의과대학) ;
  • 김형우 (부산대학교 한의학전문대학원)
  • Received : 2012.08.20
  • Accepted : 2012.10.16
  • Published : 2012.10.25
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Abstract

This study was designed to investigate the effects of 1-fluoro-2,4 dinitrobenzene (DNFB) on Induction of dermatitis in mice. We investigated the effects of DNFB on induction of dermatitis in terms of changes in body weights, ear thickness, ear weight, spleen/body ratio, histopathological observation and cytokine productions in inflammed tissue of contact dermatitis (CD) mice. In our experiments, we induced CD by using two different methods. First, mice were sensitized and challenged on the back of each ear (topical induction). Second, mice were sensitized on shaved back and challenged back of each ear (systemic induction). In our results, average weights were lowered in both topical and systemic group. But, there was no statistical significance between topical and systemic group. Treatment with DNFB enlarged ear weights and thicknesses in both topical and systemic groups. In addition, both groups were showed almost same features such as immune cell infiltration, spongiosis and hyperplasia in histopathological observations. Finally production levels of TNF-a, IFN-g and IL-6 were markedly elevated in both topical and systemic groups. On the other hand, systemic induction was more effective in spleen/body ratio and IL-6 production compared to topical induction respectively. These results suggest that dermatitis model using DNFB was used as model of CD, not for atopic dermatitis model.

Keywords

Acknowledgement

Supported by : 부산대학교

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