• 한국작물학회지
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• 제56권4호
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• pp.349-360
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• 2011

The objectives of present study were to characterize the peptides which were isolated from Korean fermented soybean paste, chungkukjang, and to determine their antioxidant activities. Four fractions were collected from the methanol extract of chungkukjang by using a recycling preparative HPLC. Among fractions, Fr-2 was identified to be highly potent free radical scavenging activity in the assay of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and nitroblue tetrazolium(NBT)-reduction inhibition. Base on antioxidant effects, fraction Fr-2 was employed for the refraction with a prep-column and separated into five fractions of which two fractions were identified to have higher antioxidant activity. To confirm the amino acid constituents of antioxidant fractions Fr-2-2 and Fr-2-3 were analyzed, and eight kinds of amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, lysine, histidine, and arginine were identified as the constituent amino acids. Antioxidant activities of the separated peptides were further assessed cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT), and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H2O2). Chungkukjang peptides have shown their ability to protect H4IIE rat hepatoma cells against H2O2- induced oxidative stress by concentration and time-dependent manner. Therefore, These results indicated that fermented soybean paste chungkukjang will be promoted the antioxidant and radical scavenging activities, and beneficial for health. The antioxidant peptide fractions Fr-2-2 and Fr-2-3 were denominated as P-NICS-1 and P-NICS-2, respectively. However, further studies were required to clarify their amino acid sequences and molecular properties, and physiological significances.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1402-1407
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• 2005

The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent $LC_{50}$ value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an $LC_{50}$ value of 210,20, 180, and 290 mg/l against B. cinerea, E. graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an $LC_{50}$ value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.

• Journal of Microbiology and Biotechnology
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• 제30권6호
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• pp.893-902
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• 2020

Propolis is a resinous substance that is collected by Apis mellifera from plant sources and is used in traditional medicine. To study the phytochemical constituents and apoptotic potential of Jordanian propolis extract against different cancer cell lines, propolis was extracted using methanol, hexane, and ethyl acetate and was fractionated using chromatographic methods. Cytotoxicity was assessed using MTT and LDH assays. The apoptotic potential was investigated using florescence microscopy, multicaspase assay, Annexin-V and dead cell assay, and cell cycle assay. The phytochemical constituents were analyzed using GC-MS. The methanol extract of propolis exhibited cytotoxic potential against all cell lines tested. The IC₅₀ values of the methanol extract were 47.4, 77.8, 91.2, and 145.0 ㎍/ml for HepG2, LoVo, MDAMB231, and MCF7 cell lines, respectively. The IC₅₀ values of the F1 fraction were 31.6 (MDAMB231), 38.9 (HepG2), 36.7 (LoVo) and 75.5 (MCF7) ㎍/ml. On further purification using thin-layer chromatography, the IC₅₀ values of the F1-3 fraction were found to be 84.31(HepG2), 79.2 (MCF7), 70.4 (LoVo), and 68.9 (MDAMB231) ㎍/ml, respectively. The anticancer potential of the F1 fraction was confirmed through the induction of apoptosis and cell cycle arrest at the G0/G1 phase. The GC-MS analysis of the F1 fraction revealed the presence of 3-methyl-4-isopropylphenol (29.44%) as a major constituent. These findings indicate the potential of propolis extract as a cancer therapy. However, further investigation is required to assess the acute and subacute toxicity of the most active fraction.

• 한국콘텐츠학회논문지
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• 제17권7호
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• pp.455-477
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• 2017

본 연구는 지속가능한 축제품질 척도 개발 가운데 축제 서비스품질 척도를 개발하여 결과변수인 축제만족, 행동의도 간의 영향관계를 실증적으로 검증하였다. 축제 서비스품질의 구성요인은 편의성, 정보성, 친절성, 운영성, 외형성이다. 척도의 개발의 목적은 축제 서비스는 대규모 집객환경에서 제공되는 서비스로 다양한 축제콘텐츠가 연행되는 가운데 대인서비스가 제공되고 있으므로 이와 같은 상황을 반영한 새로운 측정도구를 개발하기 위함이다. 본 연구에서는 척도를 개발하기 위한 분석의 틀로써 축제 서비스품질과 관련된 183편의 선행연구를 탐색하여 5개의 구성요인을 도출하였고 후속적 문헌연구를 통해 축제 서비스품질 척도를 개발하였다. 개발된 척도를 실무적으로 검증하기 위해 "화천산천어축제"에 참여한 방문객을 표본 집단으로 이용하였다. 분석결과, 축제 서비스품질 구성요인 가운데 정보성, 친절성, 운영성이 축제만족에 정(+)의 영향을 미치는 것으로 나타났다. 그리고 축제만족은 행동의도에 정(+)의 영향을 미치는 것으로 나타났다. 본 연구는 기존의 선행연구들과 달리 축제의 서비스적인 측면만을 고려하여 우리나라 지역축제의 서비스를 평가하기 위한 척도를 개발하여 실무적으로 검증하였다. 이로써 지역축제라는 특수한 환경에서 제공되는 대인 서비스를 객관적으로 평가하기 위한 세분화된 축제품질 측정의 토대를 마련하였다.

• 한국콘텐츠학회논문지
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• 제11권12호
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• pp.475-485
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• 2011

본 연구는 사이버대생과 일반대생의 사회복지 현장실습성과와 전문직 정체성에 영향을 미치는 요인을 비교하여 현장실습의 질을 제고하기 위한 각 학교의 개입지점을 찾고자 한다. 연구결과, 사이버대생의 현장 실습성과가 일반대생보다 유의미하게 더 높게 나타났으며, 사이버대생의 현장실습성과에는 수퍼비전, 학교 요인이, 일반대생은 전공만족도, 수퍼비전과 학교요인이 영향을 미치는 요인으로 나타났다. 전문직 정체성에는 사이버대생의 경우 전공만족도, 수퍼비전, 학교요인이, 일반대생은 전공만족도, 수퍼비전이 영향을 미쳤다. 이에 실습 수퍼바이저는 분명한 역할과 체계적인 지도를 제공하고, 학교는 학생들의 전공만족도를 높이기 위한 노력과 함께 예비사회복지사로서의 자질을 향상시킬 수 있는 사전 사후실습지도가 필요하다.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.679-687
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• 2018

Korean red pine (Pinus densiflora) is one of the major Pinus species in Korea. Red pine bark is removed prior to the chipping process in the wood industry and discarded as waste. However, red pine bark contains a considerable amount of naturally occurring phenolics, including flavonoids, and therefore may have a variety of biological effects. In this study, we investigated if Korean red pine bark extract (KRPBE) could protect neuronal PC-12 cells from oxidative stress and inhibit cholinesterase activity. Analysis of reversed-phase high-performance liquid chromatography results revealed four phenolics in KRPBE: vanillin, protocatechuic acid, catechin, and taxifolin. The total phenolic and flavonoid contents of KRPBE were 397.9 mg gallic acid equivalents/g dry weight (DW) and 248.7 mg catechin equivalents/g DW, respectively. The antioxidant capacities of KRPBE measured using ABTS, DPPH, and ORAC assays were 697.3, 521.8, and 2,627.7 mg vitamin C equivalents/g DW, respectively. KRPBE and its identified phenolics protected against $H_2O_2$-induced oxidative cell death in a dose-dependent manner. Acetylcholinesterase and butyrylcholinesterase, which degrade the neurotransmitter acetylcholine to terminate neurotransmission in synaptic clefts, were inhibited by treatment with KRPBE and its identified phenolics. Taken together, these results suggest that KRPBE and its constituent antioxidative phenolics are potent neuroprotective agents that can maintain cell viability under oxidative stress and inhibit cholinesterase activity.

• 방사성폐기물학회지
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• 제5권2호
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• pp.145-154
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• 2007

벤토나이트 완충재의 열수거동 실험에서는 고준위폐기물처분장 완충재로 유력하게 고려되고 있는 국산 벤토나이트를 대상으로 열수특성을 규명하고, 또 그 결과를 바탕으로 KRS 처분환경에서 벤토나이트 완충재의 장기건전성을 평가하였다. 실험결과, 벤토나이트 완충재의 열수반응은 주 구성광물인 스멕타이트의 일라이트화를 통해 진행되었으며, 온도, $K^+$농도, pH는 이러한 일라이트화에 중요한 열수반응인자 역할을 하였다. KRS 처분환경에 대한 국산벤토나이트 완충재의 장기건전성을 분석한 결과, 정상상태에서는 벤토나이트 완충재가 오랜 기간 동안 방벽재기능을 유지하였지만, 보수적인 조건에서는 약 $5{\times}10^4$년이 경과했을 때 벤토나이트 완충재를 구성하는 스멕타이트의 50%이상이 일라이트로 전환되어 방벽재로서의 팽윤능력을 상실할 수 있음을 예상할 수 있었다.

• 한국농공학회지
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• 제44권5호
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• pp.116-126
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• 2002

Discharge pattern and water quality were investigated in the drainage water from about 10 ha of groundwater-irrigated paddy field in the growing season of 2001. Total discharge quantity was about 1,117.2 mm in which about 75% was caused by management drainage due to cultural practice of paddy rice farming and the rest by rainfall runoff where total rainfall was about 515 mm. Dry-day sampling data showed wide variations in constituent concentrations with average of 26.14 mg/L, 0.37 mg/L, 3.54 mg/L at the inlet, and 43.60 mg/L, 0.34 mg/L, 3.58 mg/L at the outlet for CO $D_{cr}$ , T-P, and T-N, respectively. Wet-day sampling data demonstrated that generally CO $D_{cr}$ followed the discharge pattern and T-P was in opposite to the discharge pattern, but T-N did not show apparent pattern to the discharge. Discharge and load are in strong relationship. And based on regression equation, pollutant loads from groundwater irrigation area are estimated to be 288.34, 1.17, and 5.45 kg/ha for CO $D_{cr}$ , T-P, and T-N, respectively, which was relatively lower than the literature value from surface water irrigation area which implies that groundwater irrigation area might use less irrigation water and result in less drainage water, Therefore, total pollutant load from paddies irrigation with groundwater could be significantly lower than that with surface water. This study shows that agricultural drainage water management needs a good care of drainage outlet as well as rainfall runoff. This study was based on limited monitoring data of one year, and further monitoring and successive analysis are recommended for more generalized conclusion.

• Steel and Composite Structures
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• 제18권6호
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• pp.1493-1515
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• 2015

A new refined hyperbolic shear deformation theory (RHSDT), which involves only four unknown functions as against five in case of other shear deformation theories, is presented for the thermoelastic bending analysis of functionally graded sandwich plates. Unlike any other theory, the number of unknown functions involved is only four, as against five in case of other shear deformation theories. The theory presented is variationally consistent, does not require shear correction factor, and gives rise to transverse shear stress variation such that the transverse shear stresses vary parabolically across the thickness satisfying shear stress free surface conditions. The sandwich plate faces are assumed to have isotropic, two-constituent material distribution through the thickness, and the modulus of elasticity, Poisson's ratio of the faces, and thermal expansion coefficients are assumed to vary according to a power law distribution in terms of the volume fractions of the constituents. The core layer is still homogeneous and made of an isotropic ceramic material. Several kinds of sandwich plates are used taking into account the symmetry of the plate and the thickness of each layer. The influences played by the transverse shear deformation, thermal load, plate aspect ratio and volume fraction distribution are studied. Numerical results for deflections and stresses of functionally graded metal-ceramic plates are investigated. It can be concluded that the proposed theory is accurate and simple in solving the thermoelastic bending behavior of functionally graded plates.

• 한국분말재료학회지
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• 제18권6호
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• pp.532-537
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• 2011

Thermally stable $TiO_2$/Pt/$SiO_2$ core-shell nanocatalyst has been synthesized by chemical processes. Citrated capped Pt nanoparticles were deposited on amine functionalized silica produced by Stober process. Ultrathin layer of titania was coated on Pt/$SiO_2$ for preventing sintering of the metal nanoparticles at high temperatures. Thermal stability of the metal-oxide hybrid catalyst was demonstrated heating the sample up to $600^{\circ}C$ in air and by investigating the morphology and integrity of the structure by transmission electron spectroscopy. The surface analysis of the constituent elements was performed by X-ray photoemission spectroscopy. The catalytic activity of the hybrid catalysts was investigated by CO oxidation reaction with oxygen as a model reaction.